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Protein

Envelopment polyprotein

Gene

GP

Organism
Puumala virus (strain K27)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Glycoprotein N and glycoprotein C interact with each other and are present at the surface of the virion. They are able to attach the virion to host cell receptors. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Also promote fusion of viral membrane with host endosomal membrane after endocytosis of the virion. Glycoprotein N contains an ITAM motif which is likely to dysregulate normal immune and endothelial cell responses and contribute to virus pathogenesis (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Fusion of virus membrane with host endosomal membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host RLR pathway by virus, Inhibition of host TRAFs by virus, Viral attachment to host cell, Viral immunoevasion, Viral penetration into host cytoplasm, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Envelopment polyprotein
Alternative name(s):
M polyprotein
Cleaved into the following 2 chains:
Glycoprotein NBy similarity
Short name:
Gn
Alternative name(s):
Glycoprotein G1
Glycoprotein CBy similarity
Short name:
Gc
Alternative name(s):
Glycoprotein G2
Gene namesi
Name:GP
OrganismiPuumala virus (strain K27)
Taxonomic identifieri39000 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesBunyaviridaeHantavirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Myodes glareolus (Bank vole) (Clethrionomys glareolus) [TaxID: 447135]

Subcellular locationi

Glycoprotein N :
  • Virion membrane By similarity
  • Host Golgi apparatus membrane By similarity; Single-pass type I membrane protein By similarity
  • Host endoplasmic reticulum membrane By similarity; Single-pass type I membrane protein By similarity

  • Note: Interaction between Glycoprotein N and Glycoprotein C is essential for proper targeting of Glycoprotein N to the Golgi complex, where virion budding occurs.By similarity
Glycoprotein C :
  • Virion membrane By similarity
  • Host Golgi apparatus membrane By similarity; Single-pass type I membrane protein By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini24 – 495LumenalSequence analysisAdd BLAST472
Transmembranei496 – 516HelicalSequence analysisAdd BLAST21
Topological domaini517 – 658CytoplasmicSequence analysisAdd BLAST142
Topological domaini659 – 1114LumenalSequence analysisAdd BLAST456
Transmembranei1115 – 1135HelicalSequence analysisAdd BLAST21
Topological domaini1136 – 1148CytoplasmicSequence analysisAdd BLAST13

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host endoplasmic reticulum, Host Golgi apparatus, Host membrane, Membrane, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000003682524 – 1148Envelopment polyproteinAdd BLAST1125
ChainiPRO_000003682624 – 658Glycoprotein NBy similarityAdd BLAST635
ChainiPRO_0000036827659 – 1148Glycoprotein CBy similarityAdd BLAST490

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi142N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi357N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi409N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi898N-linked (GlcNAc...); by hostSequence analysis1
Glycosylationi937N-linked (GlcNAc...); by hostSequence analysis1

Post-translational modificationi

Specific enzymatic cleavages in vivo yield mature proteins including Glycoprotein N and Glycoprotein C.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei658 – 659Cleavage; by host signal peptidaseBy similarity2

Keywords - PTMi

Glycoprotein

Interactioni

Subunit structurei

Glycoprotein N and Glycoprotein C interacts with each other.By similarity

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini621 – 644ITAMPROSITE-ProRule annotationAdd BLAST24

Sequence similaritiesi

Contains 1 ITAM domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR016402. Envelope_glycoprot_Hantavirus.
IPR002534. Hanta_G1.
IPR002532. Hanta_G2.
IPR012316. ITAM_motif_hantavir-typ.
[Graphical view]
PfamiPF01567. Hanta_G1. 1 hit.
PF01561. Hanta_G2. 1 hit.
PF10538. ITAM_Cys-rich. 1 hit.
[Graphical view]
PIRSFiPIRSF003945. M_poly_HantaV. 1 hit.
ProDomiPD001813. Hanta_G2. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEiPS51056. ITAM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P41265-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGELSPVCLY LLLQGLLLCN TGAARNLNEL KMECPHTIRL GQGLVVGSVE
60 70 80 90 100
LPSLPIQQVE TLKLESSCNF DLHTSTAGQQ SFTKWTWEIK GDLAENTQAS
110 120 130 140 150
STSFQTKSSE VNLRGLCLIP TLVVETAARM RKTIACYDLS CNQTVCQPTV
160 170 180 190 200
YLMGPIQTCI TTKSCLLSLG DQRIQVNYEK TYCVSGQLVE GICFNPIHTM
210 220 230 240 250
ALSQPSHTYD IMTMMVRCFL VIKKVTSGDS MKIEKNFETL VQKNGCTANN
260 270 280 290 300
FQGYYICLIG SSSEPLYVPA LDDYRSAEVL SRMAFAPHGE DHDIEKNAVS
310 320 330 340 350
AMRIAGKVTG KAPSTESSDT VQGIAFSGSP LYTSTGVLTS KDDPVYIWAP
360 370 380 390 400
GIIMEGNHSI CEKKTLPLTW TGFISLPGEI EKTTQCTVFC TLAGPGADCE
410 420 430 440 450
AYSETGIFNI SSPTCSINRV QRFRGSEQQI KFVCQRVDMD ITVYCNGMKK
460 470 480 490 500
VILTKTLVIG QCIYTFTSIF SLIPGVAHSL AVELCVPGLH GWATMLLLLT
510 520 530 540 550
LCFGWVLIPT ITMILLKILI AFAYLCSKYN TDSKFRILIE KVKREYQKTM
560 570 580 590 600
GSMVCEVCQY ECETAKELES HRKSCSIGSC PYCLNPSEAT TSALQAHFKV
610 620 630 640 650
CKLTSRFQEN LRKSLTVYEP MQGCYRTLSL FRYRSRFFVG LVWCVLLVLE
660 670 680 690 700
LIVWAASAET QNLNAGWTDT AHGSGIIPMK TDLELDFSLP SSASYTYRRQ
710 720 730 740 750
LQNPANEQEK IPFHLQLSKQ VIHAEIQHLG HWMDATFNLK TAFHCYGSCE
760 770 780 790 800
KYAYPWQTAG CFIEKDYEYE TGWGCNPPDC PGVGTGCTAC GVYLDKLKSV
810 820 830 840 850
GKVFKIVSLR YTRKVCIQLG TEQTCKTVDS NDCLITTSVK VCLIGTISKF
860 870 880 890 900
QPSDTLLFLG PLQQGGLIFK QWCTTTCQFG DPGDIMSTPT GMKCPELNGS
910 920 930 940 950
FRKKCAFATT PVCQFDGNTI SGYKRMIATK DSFQSFNVTE PHISTSALEW
960 970 980 990 1000
IDPDSSLRDH INVIVSRDLS FQDLSETPCQ IDLATASIDG AWGSGVGFNL
1010 1020 1030 1040 1050
VCTVSLTECS AFLTSIKACD AAMCYGSTTA NLVRGQNTIH IVGKGGHSGS
1060 1070 1080 1090 1100
KFMCCHDTKC SSTGLVAAAP HLDRVTGYNQ ADSDKIFDDG APECGMSCWF
1110 1120 1130 1140
KKSGEWILGV LNGNWMVVAV LVVLLILSIL LFTLCCPRRP SYRKEHKP
Length:1,148
Mass (Da):126,574
Last modified:February 1, 1995 - v1
Checksum:i0AB1A245F502D265
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08754 Unassigned DNA. Translation: AAC37847.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08754 Unassigned DNA. Translation: AAC37847.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR016402. Envelope_glycoprot_Hantavirus.
IPR002534. Hanta_G1.
IPR002532. Hanta_G2.
IPR012316. ITAM_motif_hantavir-typ.
[Graphical view]
PfamiPF01567. Hanta_G1. 1 hit.
PF01561. Hanta_G2. 1 hit.
PF10538. ITAM_Cys-rich. 1 hit.
[Graphical view]
PIRSFiPIRSF003945. M_poly_HantaV. 1 hit.
ProDomiPD001813. Hanta_G2. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEiPS51056. ITAM_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGP_PUUMK
AccessioniPrimary (citable) accession number: P41265
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: January 20, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.