Reviewed, UniProtKB/Swiss-Prot P41258 (SYK_CAMJE)

Names and origin

Protein names

Recommended name:
    Lysyl-tRNA synthetase
    EC=6.1.1.6
Alternative name(s):
    Lysine--tRNA ligase
      Short name=LysRS

Gene names

Name:	lysS
Ordered Locus Names:	Cj0401

Organism

Campylobacter jejuni [Complete proteome] [HAMAP]

Taxonomic identifier

197 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	501 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys). HAMAP MF_00252
Cofactor	Binds 3 magnesium ions per subunit By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00252
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lysyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP lysine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
fliM	Q0PC73	1	EBI-1327093,EBI-1191095
fliY	Q0PC74	1	EBI-1327093,EBI-1191111

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 501	501	Lysyl-tRNA synthetase HAMAP MF_00252		PRO_0000152608
Sites
	Metal binding	404	1	Magnesium 1 By similarity
	Metal binding	411	1	Magnesium 1 By similarity
	Metal binding	411	1	Magnesium 2 By similarity
Experimental info
	Sequence conflict	19	1	N → K in AAA23029. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P41258-1 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: C8AA3D664A321728	FASTA	501	57,846
	10 20 30 40 50 60 MFDNILEQQR IEKAKELKNL GINPYPHFLE KEMSLKTFKD KFSYILEQVE KRDESVNAVV 70 80 90 100 110 120 AGRLKLLRIA GKSIFANIED EDTNLQIYFS KDSVGEELYT ILKKNLEVGD IVLVKGFPFV 130 140 150 160 170 180 TKTGEFSLHA SEVKLATKAI VPLPEKYHGL TDIEQRYRKR YVDMIMNVEV RKDFLVRSKV 190 200 210 220 230 240 VSLIRHFFEN KGFLEVETPM MHPIAGGANA KPFVTFHNSL GVERFLRIAP ELYLKRLIVG 250 260 270 280 290 300 GFEAVFEINR CFRNEGMDLT HNPEFTTIEF YWAYHNYKDL MDLTEELFAL LLDKLNLGKT 310 320 330 340 350 360 IEFDGKMINF SKPFERITYK DALCKYGGLD RDLIEDKEKI LTKLKADGFE ANEKLELGHL 370 380 390 400 410 420 QAELFDNYVE EKLINPTFVI DFPISISPLS RRSDEDSQIA ERFELFICGR ELANGFNELN 430 440 450 460 470 480 DPLDQYERFL KQIEAKNAGD EEACEMDEDF VNALGYGMPP TAGQGIGIDR LVMLLTNKKS 490 500 IRDVILFPAM RPLKSELKEK E

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Lysyl-tRNA synthetase gene of Campylobacter jejuni." Chan V.L., Bingham H.L. J. Bacteriol. 174:695-701(1992) [PubMed: 1732205] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 43431 / TGH 9011 / Serotype O:3.
[2]	"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences." Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M. , van Vliet A.H.M., Whitehead S., Barrell B.G. Nature 403:665-668(2000) [PubMed: 10688204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 11168 / Serotype O:2.
[3]	"Characterisation of a Campylobacter jejuni fur mutant." van Vliet A.H.M., Wooldridge K.G., Ketley J.M. Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-355. Strain: NCTC 11168 / Serotype O:2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M63448 Genomic DNA. Translation: AAA23029.1. AL111168 Genomic DNA. Translation: CAL34551.1. AF052056 Genomic DNA. Translation: AAC64260.1.
PIR	A42609. G81383.
RefSeq	YP_002343838.1.
3D structure databases
HSSP	HSSP built from PDB template 1BBW based on UniProtKB P13030.
ModBase	Search...
Protein-protein interaction databases
IntAct	P41258. 55 interactions.
Genome annotation databases
GeneID	904725.
GenomeReviews	Gene locus Cj0401 in contig AL111168_GR.
KEGG	cje:Cj0401.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P41258.
OMA	P41258. KIQLYVA.
Enzyme and pathway databases
BioCyc	CJEJ192222:CJ0401-MON.
BRENDA	6.1.1.6. 255835.
Family and domain databases
HAMAP	MF_00252. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-reg. IPR002313. Lys-tRNA-synth_II. IPR018149. Lys-tRNA-synth_II_C. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF4. tRNA-synt_lys_2. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR00982. TRNASYNTHLYS.
TIGRFAMs	TIGR00499. lysS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYK_CAMJE

Accession

Primary (citable) accession number: P41258
Secondary accession number(s): O68607, Q0PBA9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	December 1, 2000
Last modified:	June 16, 2009
This is version 74 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)