Isoleucine--tRNA ligase - Campylobacter jejuni subsp. jejuni serotype O:2 (strain NCTC 11168)

Contribute

Send feedback

Read comments (?) or add your own

P41257 (SYI_CAMJE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 103. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Isoleucine--tRNA ligase
EC=6.1.1.5

Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS

Gene names

Name:	ileS
Ordered Locus Names:	Cj1061c

Organism

Campylobacter jejuni subsp. jejuni serotype O:2 (strain NCTC 11168) [Reference proteome] [HAMAP]

Taxonomic identifier

192222 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter ›

Protein attributes

Sequence length	917 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP-Rule MF_02002
Catalytic activity	ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02002
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_02002
Subunit structure	Monomer By similarity. HAMAP-Rule MF_02002
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_02002.
Domain	IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP-Rule MF_02002
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	isoleucyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP regulation of translational fidelity Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA editing activity Inferred from electronic annotation. Source: InterPro isoleucine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 917

917

Isoleucine--tRNA ligase HAMAP-Rule MF_02002

PRO_0000098372

Regions

Motif

59 – 69

"HIGH" region HAMAP-Rule MF_02002

Motif

610 – 614

"KMSKS" region HAMAP-Rule MF_02002

Sites

Metal binding

890

Zinc By similarity

Metal binding

893

Zinc By similarity

Metal binding

905

Zinc By similarity

Metal binding

908

Zinc By similarity

Binding site

569

Aminoacyl-adenylate By similarity

Binding site

613

ATP By similarity

Experimental info

Sequence conflict

144

D → N in AAA91296. Ref.1

Sequence conflict

223

E → K in AAA91296. Ref.1

Sequence conflict

362

G → R in AAA91296. Ref.1

Sequence conflict

527

K → N in AAA91296. Ref.1

Sequence conflict

592

A → T in AAA91296. Ref.1

Sequence conflict

621

S → P in AAA91296. Ref.1

Sequence conflict

697

T → S in AAA91296. Ref.1

Sequence conflict

759

M → I in AAA91296. Ref.1

Sequence conflict

769

P → S in AAA91296. Ref.1

Sequence conflict

816

A → V in AAA91296. Ref.1

Sequence conflict

900

N → D in AAA91296. Ref.1

Sequence conflict

915

V → I in AAA91296. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P41257 [UniParc].

Last modified December 1, 2000. Version 2.
Checksum: 73E379C8F8F3D6EA
Show »

FASTA

917

105,262

        10         20         30         40         50         60 
MDYKETLLLP STTFAMRANL AELEPQRFKK WFEQNYAYEK MKENRKNAKK SFTLHDGPPY 

        70         80         90        100        110        120 
ANGHIHIGHA LNKILKETII KTHYFKGESV RFTPGWDCHG LPIEQQVEVK LGEKKKSLSK 

       130        140        150        160        170        180 
KEIREFCRQH ASEFVDIQRE EFKDLGIIAD WDKPYLTMKF EFEAAIYRTL CEIAKKGLLC 

       190        200        210        220        230        240 
ERSKPVFWSW AAKSALAEAE VEYQDKEDYS IFVAFDLDVK ACEKLGVSKA SAVIWTTTPW 

       250        260        270        280        290        300 
TLVANQAIAL NPNENYVITK EGLIFASALL KSMVEKGLTS GEIQKELNAK EFEKLEAINP 

       310        320        330        340        350        360 
LNGRKSVLIM GEHVLMDGGS GLVHTAPGHG EDDYYACLKY GIEVLMPVDD SGCYDETLRA 

       370        380        390        400        410        420 
KGLLPSHLLE EFIGLHIFKA NEKILELLGE KLLHSSKFIH SYPFCWRTHK PVIYRATKQW 

       430        440        450        460        470        480 
FILMDEPKLQ GKTLRECAKE QLLKTTFYPQ SGVKRIGSMV ENRPDWCISR QRDWGTPIAF 

       490        500        510        520        530        540 
FRDKNTKEVI FDDELFDFVA AIFEKHGADA WWEFEIKDLI PTNSKYKAEN LEKVYDILDV 

       550        560        570        580        590        600 
WFDSGSTFNA VLNSGLYDAG EKRASMYLEG SDQHRGWFQS SLLVGTAINE SAPYESILTH 

       610        620        630        640        650        660 
GFTTDEKGQK MSKSKGNVIA SEYVAKTYGV EILRLWILLS DYSSDLKISD NILKQVGEQY 

       670        680        690        700        710        720 
RKIRNTIRFL LANTNDLKDL EVKEFSFIDK WILSRATKVF KASKEAFFAY EFAKGFSLLL 

       730        740        750        760        770        780 
NFLSADLSGI YLDISKDRLY CDSENAQRRK SAQVAMALMA KELLNLLAPN LSYSVDEALE 

       790        800        810        820        830        840 
HANVLIKGDA KDVFDLSLTQ DFDYDFGIDD TFLMSAREKF FEQIDILKKD KIIKSTLELN 

       850        860        870        880        890        900 
LNISFNKFPN EELADWFMVS QISNENEEIL AEFEVENEKF KITKASLCKC PRCWKLQSKN 

       910 
EETPCLRCEE VLKGVQC

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An isoleucyl-tRNA synthetase gene from Campylobacter jejuni." Hong Y., Wong T., Bourke B., Chan V.L. Microbiology 141:2561-2567(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 43431 / TGH 9011 / Serotype O:3.
[2]	"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences." Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M. , van Vliet A.H.M., Whitehead S., Barrell B.G. Nature 403:665-668(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 11168.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U15295 Genomic DNA. Translation: AAA91296.1. AL111168 Genomic DNA. Translation: CAL35179.1.
PIR	B81309.
RefSeq	YP_002344456.1. NC_002163.1.
3D structure databases
ProteinModelPortal	P41257.
ModBase	Search...
Protein-protein interaction databases
IntAct	P41257. 13 interactions.
STRING	192222.Cj1061c.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAL35179; CAL35179; Cj1061c.
GeneID	905353.
KEGG	cje:Cj1061c.
PATRIC	20059080. VBICamJej33762_1043.
Phylogenomic databases
eggNOG	COG0060.
HOGENOM	HOG000246402.
KO	K01870.
OMA	KQVLTHG.
ProtClustDB	PRK05743.
Enzyme and pathway databases
BioCyc	CJEJ192222:GJTS-1035-MONOMER.
Family and domain databases
Gene3D	3.40.50.620. 2 hits. 3.90.740.10. 1 hit.
HAMAP	MF_02002. Ile_tRNA_synth_type1.
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR002300. aa-tRNA-synth_Ia. IPR002301. Ile-tRNA-ligase. IPR023585. Ile-tRNA-ligase_type1. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. IPR013155. V/L/I-tRNA-synth_anticodon-bd. IPR009008. Val/Leu/Ile-tRNA-synth_edit. [Graphical view]
PANTHER	PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfam	PF08264. Anticodon_1. 1 hit. PF00133. tRNA-synt_1. 1 hit. [Graphical view]
PRINTS	PR00984. TRNASYNTHILE.
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit. SSF50677. ValRS_IleRS_edit. 1 hit.
TIGRFAMs	TIGR00392. ileS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYI_CAMJE

Accession

Primary (citable) accession number: P41257
Secondary accession number(s): Q0P9J1, Q9PNN0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	December 1, 2000
Last modified:	May 1, 2013
This is version 103 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents