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P41257

- SYI_CAMJE

UniProt

P41257 - SYI_CAMJE

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Protein
Isoleucine--tRNA ligase
Gene
ileS, Cj1061c
Organism
Campylobacter jejuni subsp. jejuni serotype O:2 (strain NCTC 11168)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei569 – 5691Aminoacyl-adenylate By similarity
Binding sitei613 – 6131ATP By similarity
Metal bindingi890 – 8901Zinc By similarity
Metal bindingi893 – 8931Zinc By similarity
Metal bindingi905 – 9051Zinc By similarity
Metal bindingi908 – 9081Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCJEJ192222:GJTS-1035-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Cj1061c
OrganismiCampylobacter jejuni subsp. jejuni serotype O:2 (strain NCTC 11168)
Taxonomic identifieri192222 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
ProteomesiUP000000799: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 917917Isoleucine--tRNA ligaseUniRule annotation
PRO_0000098372Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

IntActiP41257. 13 interactions.
STRINGi192222.Cj1061c.

Structurei

3D structure databases

ProteinModelPortaliP41257.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi59 – 6911"HIGH" regionUniRule annotation
Add
BLAST
Motifi610 – 6145"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P41257-1 [UniParc]FASTAAdd to Basket

« Hide

MDYKETLLLP STTFAMRANL AELEPQRFKK WFEQNYAYEK MKENRKNAKK    50
SFTLHDGPPY ANGHIHIGHA LNKILKETII KTHYFKGESV RFTPGWDCHG 100
LPIEQQVEVK LGEKKKSLSK KEIREFCRQH ASEFVDIQRE EFKDLGIIAD 150
WDKPYLTMKF EFEAAIYRTL CEIAKKGLLC ERSKPVFWSW AAKSALAEAE 200
VEYQDKEDYS IFVAFDLDVK ACEKLGVSKA SAVIWTTTPW TLVANQAIAL 250
NPNENYVITK EGLIFASALL KSMVEKGLTS GEIQKELNAK EFEKLEAINP 300
LNGRKSVLIM GEHVLMDGGS GLVHTAPGHG EDDYYACLKY GIEVLMPVDD 350
SGCYDETLRA KGLLPSHLLE EFIGLHIFKA NEKILELLGE KLLHSSKFIH 400
SYPFCWRTHK PVIYRATKQW FILMDEPKLQ GKTLRECAKE QLLKTTFYPQ 450
SGVKRIGSMV ENRPDWCISR QRDWGTPIAF FRDKNTKEVI FDDELFDFVA 500
AIFEKHGADA WWEFEIKDLI PTNSKYKAEN LEKVYDILDV WFDSGSTFNA 550
VLNSGLYDAG EKRASMYLEG SDQHRGWFQS SLLVGTAINE SAPYESILTH 600
GFTTDEKGQK MSKSKGNVIA SEYVAKTYGV EILRLWILLS DYSSDLKISD 650
NILKQVGEQY RKIRNTIRFL LANTNDLKDL EVKEFSFIDK WILSRATKVF 700
KASKEAFFAY EFAKGFSLLL NFLSADLSGI YLDISKDRLY CDSENAQRRK 750
SAQVAMALMA KELLNLLAPN LSYSVDEALE HANVLIKGDA KDVFDLSLTQ 800
DFDYDFGIDD TFLMSAREKF FEQIDILKKD KIIKSTLELN LNISFNKFPN 850
EELADWFMVS QISNENEEIL AEFEVENEKF KITKASLCKC PRCWKLQSKN 900
EETPCLRCEE VLKGVQC 917
Length:917
Mass (Da):105,262
Last modified:December 1, 2000 - v2
Checksum:i73E379C8F8F3D6EA
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti144 – 1441D → N in AAA91296. 1 Publication
Sequence conflicti223 – 2231E → K in AAA91296. 1 Publication
Sequence conflicti362 – 3621G → R in AAA91296. 1 Publication
Sequence conflicti527 – 5271K → N in AAA91296. 1 Publication
Sequence conflicti592 – 5921A → T in AAA91296. 1 Publication
Sequence conflicti621 – 6211S → P in AAA91296. 1 Publication
Sequence conflicti697 – 6971T → S in AAA91296. 1 Publication
Sequence conflicti759 – 7591M → I in AAA91296. 1 Publication
Sequence conflicti769 – 7691P → S in AAA91296. 1 Publication
Sequence conflicti816 – 8161A → V in AAA91296. 1 Publication
Sequence conflicti900 – 9001N → D in AAA91296. 1 Publication
Sequence conflicti915 – 9151V → I in AAA91296. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U15295 Genomic DNA. Translation: AAA91296.1.
AL111168 Genomic DNA. Translation: CAL35179.1.
PIRiB81309.
RefSeqiYP_002344456.1. NC_002163.1.

Genome annotation databases

EnsemblBacteriaiCAL35179; CAL35179; Cj1061c.
GeneIDi905353.
KEGGicje:Cj1061c.
PATRICi20059080. VBICamJej33762_1043.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U15295 Genomic DNA. Translation: AAA91296.1 .
AL111168 Genomic DNA. Translation: CAL35179.1 .
PIRi B81309.
RefSeqi YP_002344456.1. NC_002163.1.

3D structure databases

ProteinModelPortali P41257.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P41257. 13 interactions.
STRINGi 192222.Cj1061c.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAL35179 ; CAL35179 ; Cj1061c .
GeneIDi 905353.
KEGGi cje:Cj1061c.
PATRICi 20059080. VBICamJej33762_1043.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci CJEJ192222:GJTS-1035-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "An isoleucyl-tRNA synthetase gene from Campylobacter jejuni."
    Hong Y., Wong T., Bourke B., Chan V.L.
    Microbiology 141:2561-2567(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 43431 / TGH 9011 / Serotype O:3.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NCTC 11168.

Entry informationi

Entry nameiSYI_CAMJE
AccessioniPrimary (citable) accession number: P41257
Secondary accession number(s): Q0P9J1, Q9PNN0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: December 1, 2000
Last modified: May 14, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi