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P41257 (SYI_CAMJE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase

EC=6.1.1.5
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS
Gene names
Name:ileS
Ordered Locus Names:Cj1061c
OrganismCampylobacter jejuni subsp. jejuni serotype O:2 (strain NCTC 11168) [Reference proteome] [HAMAP]
Taxonomic identifier192222 [NCBI]
Taxonomic lineageBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter

Protein attributes

Sequence length917 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP-Rule MF_02002

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02002

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_02002

Subunit structure

Monomer By similarity. HAMAP-Rule MF_02002

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_02002.

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP-Rule MF_02002

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processisoleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

isoleucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 917917Isoleucine--tRNA ligase HAMAP-Rule MF_02002
PRO_0000098372

Regions

Motif59 – 6911"HIGH" region HAMAP-Rule MF_02002
Motif610 – 6145"KMSKS" region HAMAP-Rule MF_02002

Sites

Metal binding8901Zinc By similarity
Metal binding8931Zinc By similarity
Metal binding9051Zinc By similarity
Metal binding9081Zinc By similarity
Binding site5691Aminoacyl-adenylate By similarity
Binding site6131ATP By similarity

Experimental info

Sequence conflict1441D → N in AAA91296. Ref.1
Sequence conflict2231E → K in AAA91296. Ref.1
Sequence conflict3621G → R in AAA91296. Ref.1
Sequence conflict5271K → N in AAA91296. Ref.1
Sequence conflict5921A → T in AAA91296. Ref.1
Sequence conflict6211S → P in AAA91296. Ref.1
Sequence conflict6971T → S in AAA91296. Ref.1
Sequence conflict7591M → I in AAA91296. Ref.1
Sequence conflict7691P → S in AAA91296. Ref.1
Sequence conflict8161A → V in AAA91296. Ref.1
Sequence conflict9001N → D in AAA91296. Ref.1
Sequence conflict9151V → I in AAA91296. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P41257 [UniParc].

Last modified December 1, 2000. Version 2.
Checksum: 73E379C8F8F3D6EA

FASTA917105,262
        10         20         30         40         50         60 
MDYKETLLLP STTFAMRANL AELEPQRFKK WFEQNYAYEK MKENRKNAKK SFTLHDGPPY 

        70         80         90        100        110        120 
ANGHIHIGHA LNKILKETII KTHYFKGESV RFTPGWDCHG LPIEQQVEVK LGEKKKSLSK 

       130        140        150        160        170        180 
KEIREFCRQH ASEFVDIQRE EFKDLGIIAD WDKPYLTMKF EFEAAIYRTL CEIAKKGLLC 

       190        200        210        220        230        240 
ERSKPVFWSW AAKSALAEAE VEYQDKEDYS IFVAFDLDVK ACEKLGVSKA SAVIWTTTPW 

       250        260        270        280        290        300 
TLVANQAIAL NPNENYVITK EGLIFASALL KSMVEKGLTS GEIQKELNAK EFEKLEAINP 

       310        320        330        340        350        360 
LNGRKSVLIM GEHVLMDGGS GLVHTAPGHG EDDYYACLKY GIEVLMPVDD SGCYDETLRA 

       370        380        390        400        410        420 
KGLLPSHLLE EFIGLHIFKA NEKILELLGE KLLHSSKFIH SYPFCWRTHK PVIYRATKQW 

       430        440        450        460        470        480 
FILMDEPKLQ GKTLRECAKE QLLKTTFYPQ SGVKRIGSMV ENRPDWCISR QRDWGTPIAF 

       490        500        510        520        530        540 
FRDKNTKEVI FDDELFDFVA AIFEKHGADA WWEFEIKDLI PTNSKYKAEN LEKVYDILDV 

       550        560        570        580        590        600 
WFDSGSTFNA VLNSGLYDAG EKRASMYLEG SDQHRGWFQS SLLVGTAINE SAPYESILTH 

       610        620        630        640        650        660 
GFTTDEKGQK MSKSKGNVIA SEYVAKTYGV EILRLWILLS DYSSDLKISD NILKQVGEQY 

       670        680        690        700        710        720 
RKIRNTIRFL LANTNDLKDL EVKEFSFIDK WILSRATKVF KASKEAFFAY EFAKGFSLLL 

       730        740        750        760        770        780 
NFLSADLSGI YLDISKDRLY CDSENAQRRK SAQVAMALMA KELLNLLAPN LSYSVDEALE 

       790        800        810        820        830        840 
HANVLIKGDA KDVFDLSLTQ DFDYDFGIDD TFLMSAREKF FEQIDILKKD KIIKSTLELN 

       850        860        870        880        890        900 
LNISFNKFPN EELADWFMVS QISNENEEIL AEFEVENEKF KITKASLCKC PRCWKLQSKN 

       910 
EETPCLRCEE VLKGVQC 

« Hide

References

« Hide 'large scale' references
[1]"An isoleucyl-tRNA synthetase gene from Campylobacter jejuni."
Hong Y., Wong T., Bourke B., Chan V.L.
Microbiology 141:2561-2567(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 43431 / TGH 9011 / Serotype O:3.
[2]"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences."
Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M. expand/collapse author list , van Vliet A.H.M., Whitehead S., Barrell B.G.
Nature 403:665-668(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: NCTC 11168.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U15295 Genomic DNA. Translation: AAA91296.1.
AL111168 Genomic DNA. Translation: CAL35179.1.
PIRB81309.
RefSeqYP_002344456.1. NC_002163.1.

3D structure databases

ProteinModelPortalP41257.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP41257. 13 interactions.
STRING192222.Cj1061c.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAL35179; CAL35179; Cj1061c.
GeneID905353.
KEGGcje:Cj1061c.
PATRIC20059080. VBICamJej33762_1043.

Phylogenomic databases

eggNOGCOG0060.
HOGENOMHOG000246402.
KOK01870.
OMAKPVHWCL.
OrthoDBEOG644ZM1.

Enzyme and pathway databases

BioCycCJEJ192222:GJTS-1035-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02002. Ile_tRNA_synth_type1.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00392. ileS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYI_CAMJE
AccessionPrimary (citable) accession number: P41257
Secondary accession number(s): Q0P9J1, Q9PNN0
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: December 1, 2000
Last modified: May 14, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries