Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P41257

- SYI_CAMJE

UniProt

P41257 - SYI_CAMJE

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Campylobacter jejuni subsp. jejuni serotype O:2 (strain NCTC 11168)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 2 (01 Dec 2000)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei569 – 5691Aminoacyl-adenylateUniRule annotation
    Binding sitei613 – 6131ATPUniRule annotation
    Metal bindingi890 – 8901ZincUniRule annotation
    Metal bindingi893 – 8931ZincUniRule annotation
    Metal bindingi905 – 9051ZincUniRule annotation
    Metal bindingi908 – 9081ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciCJEJ192222:GJTS-1035-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Cj1061c
    OrganismiCampylobacter jejuni subsp. jejuni serotype O:2 (strain NCTC 11168)
    Taxonomic identifieri192222 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
    ProteomesiUP000000799: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 917917Isoleucine--tRNA ligasePRO_0000098372Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    IntActiP41257. 13 interactions.
    STRINGi192222.Cj1061c.

    Structurei

    3D structure databases

    ProteinModelPortaliP41257.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi59 – 6911"HIGH" regionAdd
    BLAST
    Motifi610 – 6145"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P41257-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDYKETLLLP STTFAMRANL AELEPQRFKK WFEQNYAYEK MKENRKNAKK    50
    SFTLHDGPPY ANGHIHIGHA LNKILKETII KTHYFKGESV RFTPGWDCHG 100
    LPIEQQVEVK LGEKKKSLSK KEIREFCRQH ASEFVDIQRE EFKDLGIIAD 150
    WDKPYLTMKF EFEAAIYRTL CEIAKKGLLC ERSKPVFWSW AAKSALAEAE 200
    VEYQDKEDYS IFVAFDLDVK ACEKLGVSKA SAVIWTTTPW TLVANQAIAL 250
    NPNENYVITK EGLIFASALL KSMVEKGLTS GEIQKELNAK EFEKLEAINP 300
    LNGRKSVLIM GEHVLMDGGS GLVHTAPGHG EDDYYACLKY GIEVLMPVDD 350
    SGCYDETLRA KGLLPSHLLE EFIGLHIFKA NEKILELLGE KLLHSSKFIH 400
    SYPFCWRTHK PVIYRATKQW FILMDEPKLQ GKTLRECAKE QLLKTTFYPQ 450
    SGVKRIGSMV ENRPDWCISR QRDWGTPIAF FRDKNTKEVI FDDELFDFVA 500
    AIFEKHGADA WWEFEIKDLI PTNSKYKAEN LEKVYDILDV WFDSGSTFNA 550
    VLNSGLYDAG EKRASMYLEG SDQHRGWFQS SLLVGTAINE SAPYESILTH 600
    GFTTDEKGQK MSKSKGNVIA SEYVAKTYGV EILRLWILLS DYSSDLKISD 650
    NILKQVGEQY RKIRNTIRFL LANTNDLKDL EVKEFSFIDK WILSRATKVF 700
    KASKEAFFAY EFAKGFSLLL NFLSADLSGI YLDISKDRLY CDSENAQRRK 750
    SAQVAMALMA KELLNLLAPN LSYSVDEALE HANVLIKGDA KDVFDLSLTQ 800
    DFDYDFGIDD TFLMSAREKF FEQIDILKKD KIIKSTLELN LNISFNKFPN 850
    EELADWFMVS QISNENEEIL AEFEVENEKF KITKASLCKC PRCWKLQSKN 900
    EETPCLRCEE VLKGVQC 917
    Length:917
    Mass (Da):105,262
    Last modified:December 1, 2000 - v2
    Checksum:i73E379C8F8F3D6EA
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti144 – 1441D → N in AAA91296. (PubMed:7582016)Curated
    Sequence conflicti223 – 2231E → K in AAA91296. (PubMed:7582016)Curated
    Sequence conflicti362 – 3621G → R in AAA91296. (PubMed:7582016)Curated
    Sequence conflicti527 – 5271K → N in AAA91296. (PubMed:7582016)Curated
    Sequence conflicti592 – 5921A → T in AAA91296. (PubMed:7582016)Curated
    Sequence conflicti621 – 6211S → P in AAA91296. (PubMed:7582016)Curated
    Sequence conflicti697 – 6971T → S in AAA91296. (PubMed:7582016)Curated
    Sequence conflicti759 – 7591M → I in AAA91296. (PubMed:7582016)Curated
    Sequence conflicti769 – 7691P → S in AAA91296. (PubMed:7582016)Curated
    Sequence conflicti816 – 8161A → V in AAA91296. (PubMed:7582016)Curated
    Sequence conflicti900 – 9001N → D in AAA91296. (PubMed:7582016)Curated
    Sequence conflicti915 – 9151V → I in AAA91296. (PubMed:7582016)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U15295 Genomic DNA. Translation: AAA91296.1.
    AL111168 Genomic DNA. Translation: CAL35179.1.
    PIRiB81309.
    RefSeqiYP_002344456.1. NC_002163.1.

    Genome annotation databases

    EnsemblBacteriaiCAL35179; CAL35179; Cj1061c.
    GeneIDi905353.
    KEGGicje:Cj1061c.
    PATRICi20059080. VBICamJej33762_1043.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U15295 Genomic DNA. Translation: AAA91296.1 .
    AL111168 Genomic DNA. Translation: CAL35179.1 .
    PIRi B81309.
    RefSeqi YP_002344456.1. NC_002163.1.

    3D structure databases

    ProteinModelPortali P41257.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P41257. 13 interactions.
    STRINGi 192222.Cj1061c.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAL35179 ; CAL35179 ; Cj1061c .
    GeneIDi 905353.
    KEGGi cje:Cj1061c.
    PATRICi 20059080. VBICamJej33762_1043.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci CJEJ192222:GJTS-1035-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "An isoleucyl-tRNA synthetase gene from Campylobacter jejuni."
      Hong Y., Wong T., Bourke B., Chan V.L.
      Microbiology 141:2561-2567(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 43431 / TGH 9011 / Serotype O:3.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NCTC 11168.

    Entry informationi

    Entry nameiSYI_CAMJE
    AccessioniPrimary (citable) accession number: P41257
    Secondary accession number(s): Q0P9J1, Q9PNN0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 109 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3