Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P41251

- NRAM1_MOUSE

UniProt

P41251 - NRAM1_MOUSE

Protein

Natural resistance-associated macrophage protein 1

Gene

Slc11a1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Divalent transition metal (iron and manganese) transporter involved in iron metabolism and host resistance to certain pathogens. Macrophage-specific membrane transport function. Controls natural resistance to infection with intracellular parasites. Pathogen resistance involves sequestration of Fe2+ and Mn2+, cofactors of both prokaryotic and eukaryotic catalases and superoxide dismutases, not only to protect the macrophage against its own generation of reactive oxygen species, but to deny the cations to the pathogen for synthesis of its protective enzymes.

    GO - Molecular functioni

    1. manganese ion transmembrane transporter activity Source: MGI
    2. metal ion:proton antiporter activity Source: Ensembl

    GO - Biological processi

    1. activation of protein kinase activity Source: MGI
    2. antigen processing and presentation of peptide antigen Source: MGI
    3. cadmium ion transmembrane transport Source: Ensembl
    4. cellular cadmium ion homeostasis Source: Ensembl
    5. cellular iron ion homeostasis Source: MGI
    6. defense response to bacterium Source: MGI
    7. defense response to Gram-negative bacterium Source: MGI
    8. defense response to protozoan Source: MGI
    9. divalent metal ion export Source: BHF-UCL
    10. inflammatory response Source: MGI
    11. interleukin-2 production Source: MGI
    12. interleukin-3 production Source: MGI
    13. iron ion homeostasis Source: MGI
    14. iron ion transport Source: MGI
    15. L-arginine import Source: MGI
    16. macrophage activation Source: MGI
    17. manganese ion transmembrane transport Source: GOC
    18. manganese ion transport Source: MGI
    19. MAPK cascade Source: MGI
    20. MHC class II biosynthetic process Source: MGI
    21. mRNA stabilization Source: MGI
    22. multicellular organismal iron ion homeostasis Source: MGI
    23. negative regulation of cytokine production Source: MGI
    24. nitrite transport Source: MGI
    25. phagocytosis Source: MGI
    26. positive regulation of cytokine production Source: MGI
    27. positive regulation of dendritic cell antigen processing and presentation Source: MGI
    28. positive regulation of gene expression Source: MGI
    29. positive regulation of interferon-gamma production Source: MGI
    30. positive regulation of phagocytosis Source: MGI
    31. positive regulation of T-helper 1 type immune response Source: MGI
    32. positive regulation of transcription from RNA polymerase II promoter Source: MGI
    33. protein import into nucleus, translocation Source: MGI
    34. respiratory burst Source: MGI
    35. response to bacterium Source: MGI
    36. response to interferon-gamma Source: MGI
    37. response to lipopolysaccharide Source: MGI
    38. T cell cytokine production Source: MGI
    39. T cell proliferation involved in immune response Source: MGI
    40. vacuolar acidification Source: MGI
    41. wound healing Source: MGI

    Keywords - Biological processi

    Ion transport, Iron transport, Transport

    Keywords - Ligandi

    Iron

    Enzyme and pathway databases

    ReactomeiREACT_198345. Phagosomal maturation (early endosomal stage).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Natural resistance-associated macrophage protein 1
    Short name:
    NRAMP 1
    Alternative name(s):
    Solute carrier family 11 member 1
    Gene namesi
    Name:Slc11a1
    Synonyms:Bcg, Ity, Lsh, Nramp1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:1345275. Slc11a1.

    Subcellular locationi

    GO - Cellular componenti

    1. cell outer membrane Source: MGI
    2. endosome membrane Source: MGI
    3. integral component of membrane Source: UniProtKB-KW
    4. late endosome Source: MGI
    5. lysosome Source: MGI
    6. phagocytic vesicle membrane Source: MGI
    7. plasma membrane Source: Ensembl
    8. tertiary granule membrane Source: Ensembl

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 548548Natural resistance-associated macrophage protein 1PRO_0000212589Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi321 – 3211N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi335 – 3351N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiP41251.
    PRIDEiP41251.

    PTM databases

    PhosphoSiteiP41251.

    Expressioni

    Tissue specificityi

    Macrophages; spleen and liver.

    Developmental stagei

    Expressed in the early stages of infection.

    Inductioni

    In response to lymphokine or bacterial products.

    Gene expression databases

    BgeeiP41251.
    GenevestigatoriP41251.

    Interactioni

    Protein-protein interaction databases

    BioGridi201840. 5 interactions.
    STRINGi10090.ENSMUSP00000027368.

    Structurei

    3D structure databases

    ProteinModelPortaliP41251.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 5555CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini74 – 829ExtracellularSequence Analysis
    Topological domaini103 – 13937CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini161 – 1644ExtracellularSequence Analysis
    Topological domaini185 – 1939CytoplasmicSequence Analysis
    Topological domaini215 – 23723ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini257 – 28428CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini305 – 34642ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini367 – 39731CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini416 – 42611ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini448 – 46316CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini486 – 4938ExtracellularSequence Analysis
    Topological domaini514 – 54835CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei56 – 7318HelicalSequence AnalysisAdd
    BLAST
    Transmembranei83 – 10220HelicalSequence AnalysisAdd
    BLAST
    Transmembranei140 – 16021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei165 – 18420HelicalSequence AnalysisAdd
    BLAST
    Transmembranei194 – 21421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei238 – 25619HelicalSequence AnalysisAdd
    BLAST
    Transmembranei285 – 30420HelicalSequence AnalysisAdd
    BLAST
    Transmembranei347 – 36620HelicalSequence AnalysisAdd
    BLAST
    Transmembranei398 – 41518HelicalSequence AnalysisAdd
    BLAST
    Transmembranei427 – 44721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei464 – 48522HelicalSequence AnalysisAdd
    BLAST
    Transmembranei494 – 51320HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi3 – 7775Pro/Ser-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the NRAMP family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1914.
    GeneTreeiENSGT00390000006526.
    HOGENOMiHOG000152203.
    HOVERGENiHBG052665.
    InParanoidiQ3TB84.
    KOiK12347.
    OMAiSHKHFLY.
    OrthoDBiEOG77127K.
    TreeFamiTF315185.

    Family and domain databases

    HAMAPiMF_00221. NRAMP.
    InterProiIPR001046. NRAMP_fam.
    [Graphical view]
    PANTHERiPTHR11706. PTHR11706. 1 hit.
    PfamiPF01566. Nramp. 1 hit.
    [Graphical view]
    PRINTSiPR00447. NATRESASSCMP.
    TIGRFAMsiTIGR01197. nramp. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P41251-1 [UniParc]FASTAAdd to Basket

    « Hide

    MISDKSPPRL SRPSYGSISS LPGPAPQPAP CRETYLSEKI PIPSADQGTF    50
    SLRKLWAFTG PGFLMSIAFL DPGNIESDLQ AGAVAGFKLL WVLLWATVLG 100
    LLCQRLAARL GVVTGKDLGE VCHLYYPKVP RILLWLTIEL AIVGSDMQEV 150
    IGTAISFNLL SAGRIPLWDG VLITIVDTFF FLFLDNYGLR KLEAFFGLLI 200
    TIMALTFGYE YVVAHPSQGA LLKGLVLPTC PGCGQPELLQ AVGIVGAIIM 250
    PHNIYLHSAL VKSREVDRTR RVDVREANMY FLIEATIALS VSFIINLFVM 300
    AVFGQAFYQQ TNEEAFNICA NSSLQNYAKI FPRDNNTVSV DIYQGGVILG 350
    CLFGPAALYI WAVGLLAAGQ SSTMTGTYAG QFVMEGFLKL RWSRFARVLL 400
    TRSCAILPTV LVAVFRDLKD LSGLNDLLNV LQSLLLPFAV LPILTFTSMP 450
    AVMQEFANGR MSKAITSCIM ALVCAINLYF VISYLPSLPH PAYFGLVALF 500
    AIGYLGLTAY LAWTCCIAHG ATFLTHSSHK HFLYGLPNEE QGGVQGSG 548
    Length:548
    Mass (Da):59,741
    Last modified:July 27, 2011 - v2
    Checksum:iE5F0C1EC9FC0C2FD
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti169 – 1691D → G.3 Publications

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X75355 mRNA. Translation: CAA53102.1.
    S79389
    , S79360, S79361, S79362, S79364, S79365, S79367, S79395, S79396, S79369, S79375, S79380, S79381, S79386, S79387 Genomic DNA. Translation: AAB35205.2.
    AK171393 mRNA. Translation: BAE42430.1.
    BC109137 mRNA. Translation: AAI09138.1.
    BC109138 mRNA. Translation: AAI09139.1.
    L13732 mRNA. Translation: AAA39838.1.
    CCDSiCCDS15047.1.
    PIRiI48693.
    RefSeqiNP_038640.2. NM_013612.2.
    UniGeneiMm.2913.

    Genome annotation databases

    EnsembliENSMUST00000027368; ENSMUSP00000027368; ENSMUSG00000026177.
    GeneIDi18173.
    KEGGimmu:18173.
    UCSCiuc007bly.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X75355 mRNA. Translation: CAA53102.1 .
    S79389
    , S79360 , S79361 , S79362 , S79364 , S79365 , S79367 , S79395 , S79396 , S79369 , S79375 , S79380 , S79381 , S79386 , S79387 Genomic DNA. Translation: AAB35205.2 .
    AK171393 mRNA. Translation: BAE42430.1 .
    BC109137 mRNA. Translation: AAI09138.1 .
    BC109138 mRNA. Translation: AAI09139.1 .
    L13732 mRNA. Translation: AAA39838.1 .
    CCDSi CCDS15047.1.
    PIRi I48693.
    RefSeqi NP_038640.2. NM_013612.2.
    UniGenei Mm.2913.

    3D structure databases

    ProteinModelPortali P41251.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201840. 5 interactions.
    STRINGi 10090.ENSMUSP00000027368.

    PTM databases

    PhosphoSitei P41251.

    Proteomic databases

    PaxDbi P41251.
    PRIDEi P41251.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000027368 ; ENSMUSP00000027368 ; ENSMUSG00000026177 .
    GeneIDi 18173.
    KEGGi mmu:18173.
    UCSCi uc007bly.2. mouse.

    Organism-specific databases

    CTDi 6556.
    MGIi MGI:1345275. Slc11a1.

    Phylogenomic databases

    eggNOGi COG1914.
    GeneTreei ENSGT00390000006526.
    HOGENOMi HOG000152203.
    HOVERGENi HBG052665.
    InParanoidi Q3TB84.
    KOi K12347.
    OMAi SHKHFLY.
    OrthoDBi EOG77127K.
    TreeFami TF315185.

    Enzyme and pathway databases

    Reactomei REACT_198345. Phagosomal maturation (early endosomal stage).

    Miscellaneous databases

    NextBioi 293474.
    PROi P41251.
    SOURCEi Search...

    Gene expression databases

    Bgeei P41251.
    Genevestigatori P41251.

    Family and domain databases

    HAMAPi MF_00221. NRAMP.
    InterProi IPR001046. NRAMP_fam.
    [Graphical view ]
    PANTHERi PTHR11706. PTHR11706. 1 hit.
    Pfami PF01566. Nramp. 1 hit.
    [Graphical view ]
    PRINTSi PR00447. NATRESASSCMP.
    TIGRFAMsi TIGR01197. nramp. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "NH2-terminal sequence of macrophage-expressed natural resistance-associated macrophage protein (Nramp) encodes a proline/serine-rich putative Src homology 3-binding domain."
      Barton C.H., White J.K., Roach T.I.A., Blackwell J.M.
      J. Exp. Med. 179:1683-1687(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT GLY-169.
      Strain: B10.L-LSH.
      Tissue: Bone marrow.
    2. "Genomic structure, promoter sequence, and induction of expression of the mouse Nramp1 gene in macrophages."
      Govoni G., Vidal S., Cellier M., Lepage P., Malo D., Gros P.
      Genomics 27:9-19(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT GLY-169.
      Strain: BALB/c.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. "Natural resistance to infection with intracellular parasites: isolation of a candidate for Bcg."
      Vidal S.M., Malo D., Vogan K., Skamene E., Gros P.
      Cell 73:469-485(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 65-548, VARIANT GLY-169.
      Strain: DBA/2.
      Tissue: Pre-B cell.

    Entry informationi

    Entry nameiNRAM1_MOUSE
    AccessioniPrimary (citable) accession number: P41251
    Secondary accession number(s): Q3TB84
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 112 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3