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Protein

Natural resistance-associated macrophage protein 1

Gene

Slc11a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Divalent transition metal (iron and manganese) transporter involved in iron metabolism and host resistance to certain pathogens. Macrophage-specific membrane transport function. Controls natural resistance to infection with intracellular parasites. Pathogen resistance involves sequestration of Fe2+ and Mn2+, cofactors of both prokaryotic and eukaryotic catalases and superoxide dismutases, not only to protect the macrophage against its own generation of reactive oxygen species, but to deny the cations to the pathogen for synthesis of its protective enzymes.

GO - Molecular functioni

GO - Biological processi

  • activation of protein kinase activity Source: MGI
  • antigen processing and presentation of peptide antigen Source: MGI
  • cadmium ion transmembrane transport Source: MGI
  • cellular cadmium ion homeostasis Source: MGI
  • cellular iron ion homeostasis Source: MGI
  • defense response to bacterium Source: MGI
  • defense response to Gram-negative bacterium Source: MGI
  • defense response to protozoan Source: MGI
  • divalent metal ion export Source: BHF-UCL
  • inflammatory response Source: MGI
  • interleukin-2 production Source: MGI
  • interleukin-3 production Source: MGI
  • iron ion homeostasis Source: MGI
  • iron ion transport Source: MGI
  • L-arginine import Source: MGI
  • macrophage activation Source: MGI
  • manganese ion transport Source: MGI
  • MAPK cascade Source: MGI
  • MHC class II biosynthetic process Source: MGI
  • mRNA stabilization Source: MGI
  • multicellular organismal iron ion homeostasis Source: MGI
  • negative regulation of cytokine production Source: MGI
  • nitrite transport Source: MGI
  • phagocytosis Source: MGI
  • positive regulation of cytokine production Source: MGI
  • positive regulation of dendritic cell antigen processing and presentation Source: MGI
  • positive regulation of gene expression Source: MGI
  • positive regulation of interferon-gamma production Source: MGI
  • positive regulation of phagocytosis Source: MGI
  • positive regulation of T-helper 1 type immune response Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • protein import into nucleus, translocation Source: MGI
  • respiratory burst Source: MGI
  • response to bacterium Source: MGI
  • response to interferon-gamma Source: MGI
  • response to lipopolysaccharide Source: MGI
  • T cell cytokine production Source: MGI
  • T cell proliferation involved in immune response Source: MGI
  • vacuolar acidification Source: MGI
  • wound healing Source: MGI
Complete GO annotation...

Keywords - Biological processi

Ion transport, Iron transport, Transport

Keywords - Ligandi

Iron

Enzyme and pathway databases

ReactomeiR-MMU-1222556. ROS, RNS production in response to bacteria.
R-MMU-425410. Metal ion SLC transporters.
R-MMU-6798695. Neutrophil degranulation.
R-MMU-6803544. Ion influx/efflux at host-pathogen interface.

Names & Taxonomyi

Protein namesi
Recommended name:
Natural resistance-associated macrophage protein 1
Short name:
NRAMP 1
Alternative name(s):
Solute carrier family 11 member 1
Gene namesi
Name:Slc11a1
Synonyms:Bcg, Ity, Lsh, Nramp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1345275. Slc11a1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 55CytoplasmicSequence analysisAdd BLAST55
Transmembranei56 – 73HelicalSequence analysisAdd BLAST18
Topological domaini74 – 82ExtracellularSequence analysis9
Transmembranei83 – 102HelicalSequence analysisAdd BLAST20
Topological domaini103 – 139CytoplasmicSequence analysisAdd BLAST37
Transmembranei140 – 160HelicalSequence analysisAdd BLAST21
Topological domaini161 – 164ExtracellularSequence analysis4
Transmembranei165 – 184HelicalSequence analysisAdd BLAST20
Topological domaini185 – 193CytoplasmicSequence analysis9
Transmembranei194 – 214HelicalSequence analysisAdd BLAST21
Topological domaini215 – 237ExtracellularSequence analysisAdd BLAST23
Transmembranei238 – 256HelicalSequence analysisAdd BLAST19
Topological domaini257 – 284CytoplasmicSequence analysisAdd BLAST28
Transmembranei285 – 304HelicalSequence analysisAdd BLAST20
Topological domaini305 – 346ExtracellularSequence analysisAdd BLAST42
Transmembranei347 – 366HelicalSequence analysisAdd BLAST20
Topological domaini367 – 397CytoplasmicSequence analysisAdd BLAST31
Transmembranei398 – 415HelicalSequence analysisAdd BLAST18
Topological domaini416 – 426ExtracellularSequence analysisAdd BLAST11
Transmembranei427 – 447HelicalSequence analysisAdd BLAST21
Topological domaini448 – 463CytoplasmicSequence analysisAdd BLAST16
Transmembranei464 – 485HelicalSequence analysisAdd BLAST22
Topological domaini486 – 493ExtracellularSequence analysis8
Transmembranei494 – 513HelicalSequence analysisAdd BLAST20
Topological domaini514 – 548CytoplasmicSequence analysisAdd BLAST35

GO - Cellular componenti

  • cell outer membrane Source: MGI
  • endosome membrane Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • late endosome Source: MGI
  • lysosome Source: MGI
  • phagocytic vesicle membrane Source: MGI
  • plasma membrane Source: MGI
  • tertiary granule membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002125891 – 548Natural resistance-associated macrophage protein 1Add BLAST548

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi321N-linked (GlcNAc...)Sequence analysis1
Glycosylationi335N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP41251.
PeptideAtlasiP41251.
PRIDEiP41251.

PTM databases

iPTMnetiP41251.
PhosphoSitePlusiP41251.

Expressioni

Tissue specificityi

Macrophages; spleen and liver.

Developmental stagei

Expressed in the early stages of infection.

Inductioni

In response to lymphokine or bacterial products.

Gene expression databases

BgeeiENSMUSG00000026177.
ExpressionAtlasiP41251. baseline and differential.
GenevisibleiP41251. MM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201840. 5 interactors.
STRINGi10090.ENSMUSP00000027368.

Structurei

3D structure databases

ProteinModelPortaliP41251.
SMRiP41251.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi3 – 77Pro/Ser-richAdd BLAST75

Sequence similaritiesi

Belongs to the NRAMP family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1291. Eukaryota.
COG1914. LUCA.
GeneTreeiENSGT00390000006526.
HOGENOMiHOG000152203.
HOVERGENiHBG052665.
InParanoidiP41251.
KOiK12347.
OMAiICPEVIN.
OrthoDBiEOG091G05M9.
TreeFamiTF315185.

Family and domain databases

HAMAPiMF_00221. NRAMP. 1 hit.
InterProiIPR001046. NRAMP_fam.
[Graphical view]
PANTHERiPTHR11706. PTHR11706. 1 hit.
PfamiPF01566. Nramp. 1 hit.
[Graphical view]
PRINTSiPR00447. NATRESASSCMP.
TIGRFAMsiTIGR01197. nramp. 1 hit.

Sequencei

Sequence statusi: Complete.

P41251-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISDKSPPRL SRPSYGSISS LPGPAPQPAP CRETYLSEKI PIPSADQGTF
60 70 80 90 100
SLRKLWAFTG PGFLMSIAFL DPGNIESDLQ AGAVAGFKLL WVLLWATVLG
110 120 130 140 150
LLCQRLAARL GVVTGKDLGE VCHLYYPKVP RILLWLTIEL AIVGSDMQEV
160 170 180 190 200
IGTAISFNLL SAGRIPLWDG VLITIVDTFF FLFLDNYGLR KLEAFFGLLI
210 220 230 240 250
TIMALTFGYE YVVAHPSQGA LLKGLVLPTC PGCGQPELLQ AVGIVGAIIM
260 270 280 290 300
PHNIYLHSAL VKSREVDRTR RVDVREANMY FLIEATIALS VSFIINLFVM
310 320 330 340 350
AVFGQAFYQQ TNEEAFNICA NSSLQNYAKI FPRDNNTVSV DIYQGGVILG
360 370 380 390 400
CLFGPAALYI WAVGLLAAGQ SSTMTGTYAG QFVMEGFLKL RWSRFARVLL
410 420 430 440 450
TRSCAILPTV LVAVFRDLKD LSGLNDLLNV LQSLLLPFAV LPILTFTSMP
460 470 480 490 500
AVMQEFANGR MSKAITSCIM ALVCAINLYF VISYLPSLPH PAYFGLVALF
510 520 530 540
AIGYLGLTAY LAWTCCIAHG ATFLTHSSHK HFLYGLPNEE QGGVQGSG
Length:548
Mass (Da):59,741
Last modified:July 27, 2011 - v2
Checksum:iE5F0C1EC9FC0C2FD
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti169D → G.3 Publications1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75355 mRNA. Translation: CAA53102.1.
S79389
, S79360, S79361, S79362, S79364, S79365, S79367, S79395, S79396, S79369, S79375, S79380, S79381, S79386, S79387 Genomic DNA. Translation: AAB35205.2.
AK171393 mRNA. Translation: BAE42430.1.
BC109137 mRNA. Translation: AAI09138.1.
BC109138 mRNA. Translation: AAI09139.1.
L13732 mRNA. Translation: AAA39838.1.
CCDSiCCDS15047.1.
PIRiI48693.
RefSeqiNP_038640.2. NM_013612.2.
UniGeneiMm.2913.

Genome annotation databases

EnsembliENSMUST00000027368; ENSMUSP00000027368; ENSMUSG00000026177.
GeneIDi18173.
KEGGimmu:18173.
UCSCiuc007bly.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75355 mRNA. Translation: CAA53102.1.
S79389
, S79360, S79361, S79362, S79364, S79365, S79367, S79395, S79396, S79369, S79375, S79380, S79381, S79386, S79387 Genomic DNA. Translation: AAB35205.2.
AK171393 mRNA. Translation: BAE42430.1.
BC109137 mRNA. Translation: AAI09138.1.
BC109138 mRNA. Translation: AAI09139.1.
L13732 mRNA. Translation: AAA39838.1.
CCDSiCCDS15047.1.
PIRiI48693.
RefSeqiNP_038640.2. NM_013612.2.
UniGeneiMm.2913.

3D structure databases

ProteinModelPortaliP41251.
SMRiP41251.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201840. 5 interactors.
STRINGi10090.ENSMUSP00000027368.

PTM databases

iPTMnetiP41251.
PhosphoSitePlusiP41251.

Proteomic databases

PaxDbiP41251.
PeptideAtlasiP41251.
PRIDEiP41251.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027368; ENSMUSP00000027368; ENSMUSG00000026177.
GeneIDi18173.
KEGGimmu:18173.
UCSCiuc007bly.2. mouse.

Organism-specific databases

CTDi6556.
MGIiMGI:1345275. Slc11a1.

Phylogenomic databases

eggNOGiKOG1291. Eukaryota.
COG1914. LUCA.
GeneTreeiENSGT00390000006526.
HOGENOMiHOG000152203.
HOVERGENiHBG052665.
InParanoidiP41251.
KOiK12347.
OMAiICPEVIN.
OrthoDBiEOG091G05M9.
TreeFamiTF315185.

Enzyme and pathway databases

ReactomeiR-MMU-1222556. ROS, RNS production in response to bacteria.
R-MMU-425410. Metal ion SLC transporters.
R-MMU-6798695. Neutrophil degranulation.
R-MMU-6803544. Ion influx/efflux at host-pathogen interface.

Miscellaneous databases

PROiP41251.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026177.
ExpressionAtlasiP41251. baseline and differential.
GenevisibleiP41251. MM.

Family and domain databases

HAMAPiMF_00221. NRAMP. 1 hit.
InterProiIPR001046. NRAMP_fam.
[Graphical view]
PANTHERiPTHR11706. PTHR11706. 1 hit.
PfamiPF01566. Nramp. 1 hit.
[Graphical view]
PRINTSiPR00447. NATRESASSCMP.
TIGRFAMsiTIGR01197. nramp. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNRAM1_MOUSE
AccessioniPrimary (citable) accession number: P41251
Secondary accession number(s): Q3TB84
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.