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P41242

- MATK_MOUSE

UniProt

P41242 - MATK_MOUSE

Protein

Megakaryocyte-associated tyrosine-protein kinase

Gene

Matk

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 2 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Could play a significant role in the signal transduction of hematopoietic cells. May regulate tyrosine kinase activity of SRC-family members in brain by specifically phosphorylating their C-terminal regulatory tyrosine residue which acts as a negative regulatory site. It may play an inhibitory role in the control of T-cell proliferation.

    Catalytic activityi

    ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei260 – 2601ATPPROSITE-ProRule annotation
    Active sitei350 – 3501Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi239 – 2479ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Kinase, Transferase, Tyrosine-protein kinase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.10.2. 3474.
    ReactomeiREACT_188191. Signaling by ERBB2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Megakaryocyte-associated tyrosine-protein kinase (EC:2.7.10.2)
    Alternative name(s):
    Protein kinase NTK
    Tyrosine-protein kinase CTK
    Gene namesi
    Name:Matk
    Synonyms:Ctk, Ntk
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 10

    Organism-specific databases

    MGIiMGI:99259. Matk.

    Subcellular locationi

    Cytoplasm 1 Publication. Membrane By similarity
    Note: In platelets, 90% of MATK localizes to the membrane fraction, and translocates to the cytoskeleton upon thrombin stimulation.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. membrane Source: UniProtKB-SubCell
    3. nucleus Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 505505Megakaryocyte-associated tyrosine-protein kinasePRO_0000088074Add
    BLAST

    Proteomic databases

    PaxDbiP41242.
    PRIDEiP41242.

    PTM databases

    PhosphoSiteiP41242.

    Expressioni

    Tissue specificityi

    Most abundant in brain, and to a lesser extent in the spleen, the thymus and the liver. Also found in the T-cell lineage.

    Gene expression databases

    ArrayExpressiP41242.
    BgeeiP41242.
    CleanExiMM_MATK.
    GenevestigatoriP41242.

    Interactioni

    Subunit structurei

    Interacts with KIT.By similarity

    Protein-protein interaction databases

    BioGridi201319. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliP41242.
    SMRiP41242. Positions 37-476.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini61 – 10848SH3PROSITE-ProRule annotationAdd
    BLAST
    Domaini120 – 20990SH2PROSITE-ProRule annotationAdd
    BLAST
    Domaini233 – 481249Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation
    Contains 1 SH2 domain.PROSITE-ProRule annotation
    Contains 1 SH3 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    SH2 domain, SH3 domain

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00750000117264.
    HOGENOMiHOG000233858.
    HOVERGENiHBG008761.
    KOiK08888.
    OrthoDBiEOG7SV0V5.
    PhylomeDBiP41242.
    TreeFamiTF351634.

    Family and domain databases

    Gene3Di3.30.505.10. 1 hit.
    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR000980. SH2.
    IPR001452. SH3_domain.
    IPR008266. Tyr_kinase_AS.
    IPR020635. Tyr_kinase_cat_dom.
    [Graphical view]
    PfamiPF07714. Pkinase_Tyr. 1 hit.
    PF00017. SH2. 1 hit.
    PF00018. SH3_1. 1 hit.
    [Graphical view]
    PRINTSiPR00401. SH2DOMAIN.
    PR00109. TYRKINASE.
    SMARTiSM00252. SH2. 1 hit.
    SM00326. SH3. 1 hit.
    SM00219. TyrKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 1 hit.
    SSF55550. SSF55550. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    PS50001. SH2. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P41242-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MARRSSRVSW LAFEGWESRD LPRVSPRLFG AWHPAPAAAR MPTRWAPGTQ    50
    CMTKCENSRP KPGELAFRKG DMVTILEACE DKSWYRAKHH GSGQEGLLAA 100
    AALRHGEALS TDPKLSLMPW FHGKISGQEA IQQLQPPEDG LFLVRESARH 150
    PGDYVLCVSF GRDVIHYRVL HRDGHLTIDE AVCFCNLMDM VEHYTKDKGA 200
    ICTKLVKPRR KQGAKSAEEE LAKAGWLLDL QHLTLGAQIG EGEFGAVLQG 250
    EYLGQKVAVK NIKCDVTAQA FLDETAVMTK LQHRNLVRLL GVILHHGLYI 300
    VMEHVSKGNL VNFLRTRGRA LVSTSQLLQF ALHVAEGMEY LESKKLVHRD 350
    LAARNILVSE DLVAKVSDFG LAKAERKGLD SSRLPVKWTA PEALKNGRFS 400
    SKSDVWSFGV LLWEVFSYGR APYPKMSLKE VSEAVEKGYR MEPPDGCPGS 450
    VHTLMGSCWE AEPARRPPFR KIVEKLGREL RSVGVSAPAG GQEAEGSAPT 500
    RSQDP 505
    Length:505
    Mass (Da):56,056
    Last modified:February 1, 1996 - v2
    Checksum:i0A1661C1FBFD6A53
    GO
    Isoform 2 (identifier: P41242-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         43-43: T → TQ

    Note: Minor isoform.

    Show »
    Length:506
    Mass (Da):56,185
    Checksum:iB77EF65C278EEB6E
    GO
    Isoform 3 (identifier: P41242-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-40: Missing.

    Show »
    Length:465
    Mass (Da):51,495
    Checksum:i6995C574F09FAE96
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti105 – 1062HG → QR(PubMed:8197166)Curated
    Sequence conflicti105 – 1062HG → QR(PubMed:7898936)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 4040Missing in isoform 3. 2 PublicationsVSP_011565Add
    BLAST
    Alternative sequencei43 – 431T → TQ in isoform 2. CuratedVSP_004966

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U05210 mRNA. Translation: AAA18829.1.
    L27738 mRNA. Translation: AAB59677.1.
    D45243 mRNA. Translation: BAA08199.1.
    L33339 Genomic DNA. Translation: AAA64431.1.
    CCDSiCCDS24048.1. [P41242-1]
    CCDS70078.1. [P41242-3]
    PIRiI48926.
    I59296.
    RefSeqiNP_001272783.1. NM_001285854.1.
    NP_001272784.1. NM_001285855.1.
    NP_034898.1. NM_010768.2.
    XP_006513358.1. XM_006513295.1.
    XP_006513362.1. XM_006513299.1.
    UniGeneiMm.2918.

    Genome annotation databases

    EnsembliENSMUST00000105328; ENSMUSP00000100965; ENSMUSG00000004933.
    ENSMUST00000117488; ENSMUSP00000113221; ENSMUSG00000004933.
    ENSMUST00000119547; ENSMUSP00000113576; ENSMUSG00000004933.
    GeneIDi17179.
    KEGGimmu:17179.
    UCSCiuc007ggv.1. mouse. [P41242-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U05210 mRNA. Translation: AAA18829.1 .
    L27738 mRNA. Translation: AAB59677.1 .
    D45243 mRNA. Translation: BAA08199.1 .
    L33339 Genomic DNA. Translation: AAA64431.1 .
    CCDSi CCDS24048.1. [P41242-1 ]
    CCDS70078.1. [P41242-3 ]
    PIRi I48926.
    I59296.
    RefSeqi NP_001272783.1. NM_001285854.1.
    NP_001272784.1. NM_001285855.1.
    NP_034898.1. NM_010768.2.
    XP_006513358.1. XM_006513295.1.
    XP_006513362.1. XM_006513299.1.
    UniGenei Mm.2918.

    3D structure databases

    ProteinModelPortali P41242.
    SMRi P41242. Positions 37-476.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201319. 1 interaction.

    PTM databases

    PhosphoSitei P41242.

    Proteomic databases

    PaxDbi P41242.
    PRIDEi P41242.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000105328 ; ENSMUSP00000100965 ; ENSMUSG00000004933 .
    ENSMUST00000117488 ; ENSMUSP00000113221 ; ENSMUSG00000004933 .
    ENSMUST00000119547 ; ENSMUSP00000113576 ; ENSMUSG00000004933 .
    GeneIDi 17179.
    KEGGi mmu:17179.
    UCSCi uc007ggv.1. mouse. [P41242-1 ]

    Organism-specific databases

    CTDi 4145.
    MGIi MGI:99259. Matk.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00750000117264.
    HOGENOMi HOG000233858.
    HOVERGENi HBG008761.
    KOi K08888.
    OrthoDBi EOG7SV0V5.
    PhylomeDBi P41242.
    TreeFami TF351634.

    Enzyme and pathway databases

    BRENDAi 2.7.10.2. 3474.
    Reactomei REACT_188191. Signaling by ERBB2.

    Miscellaneous databases

    NextBioi 291490.
    PROi P41242.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P41242.
    Bgeei P41242.
    CleanExi MM_MATK.
    Genevestigatori P41242.

    Family and domain databases

    Gene3Di 3.30.505.10. 1 hit.
    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR000980. SH2.
    IPR001452. SH3_domain.
    IPR008266. Tyr_kinase_AS.
    IPR020635. Tyr_kinase_cat_dom.
    [Graphical view ]
    Pfami PF07714. Pkinase_Tyr. 1 hit.
    PF00017. SH2. 1 hit.
    PF00018. SH3_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00401. SH2DOMAIN.
    PR00109. TYRKINASE.
    SMARTi SM00252. SH2. 1 hit.
    SM00326. SH3. 1 hit.
    SM00219. TyrKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 1 hit.
    SSF55550. SSF55550. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    PS50001. SH2. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Ctk: a protein-tyrosine kinase related to Csk that defines an enzyme family."
      Klages S., Adam D., Class K., Fargnoli J., Bolen J.B., Penhallow R.C.
      Proc. Natl. Acad. Sci. U.S.A. 91:2597-2601(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
      Tissue: Brain.
    2. "Ntk: a Csk-related protein-tyrosine kinase expressed in brain and T lymphocytes."
      Chow L.M.L., Jarvis C.D., Hu Q., Nye S.H., Gervais F.G., Veillette A., Matis L.A.
      Proc. Natl. Acad. Sci. U.S.A. 91:4975-4979(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Strain: BALB/c.
      Tissue: Thymus.
    3. "Presence of alternative 5' untranslated sequences and identification of cells expressing ctk transcripts in the brain and testis."
      Kaneko Y., Nonoguchi K., Fukuyama H., Takano S., Higashitsuji H., Nishiyama H., Takenawa J., Nakayama H., Fujita J.
      Oncogene 10:945-952(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
      Strain: DDY/STD.
    4. "Two distinct protein isoforms are encoded by ntk, a csk-related tyrosine protein kinase gene."
      Chow L.M., Davidson D., Fournel M., Gosselin P., Lemieux S., Lyu M.S., Kozak C.A., Matis L.A., Veillette A.
      Oncogene 9:3437-3448(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-43 (ISOFORM 1), ALTERNATIVE SPLICING, SUBCELLULAR LOCATION.
      Strain: 129/Sv.
      Tissue: Kidney.

    Entry informationi

    Entry nameiMATK_MOUSE
    AccessioniPrimary (citable) accession number: P41242
    Secondary accession number(s): Q6LE90
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 131 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3