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Protein

Megakaryocyte-associated tyrosine-protein kinase

Gene

Matk

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Could play a significant role in the signal transduction of hematopoietic cells. May regulate tyrosine kinase activity of SRC-family members in brain by specifically phosphorylating their C-terminal regulatory tyrosine residue which acts as a negative regulatory site. It may play an inhibitory role in the control of T-cell proliferation.

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei260 – 2601ATPPROSITE-ProRule annotation
Active sitei350 – 3501Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi239 – 2479ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.2. 3474.
ReactomeiREACT_348099. Signaling by ERBB2.

Names & Taxonomyi

Protein namesi
Recommended name:
Megakaryocyte-associated tyrosine-protein kinase (EC:2.7.10.2)
Alternative name(s):
Protein kinase NTK
Tyrosine-protein kinase CTK
Gene namesi
Name:Matk
Synonyms:Ctk, Ntk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:99259. Matk.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Membrane By similarity

  • Note: In platelets, 90% of MATK localizes to the membrane fraction, and translocates to the cytoskeleton upon thrombin stimulation.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 505505Megakaryocyte-associated tyrosine-protein kinasePRO_0000088074Add
BLAST

Proteomic databases

MaxQBiP41242.
PaxDbiP41242.
PRIDEiP41242.

PTM databases

PhosphoSiteiP41242.

Expressioni

Tissue specificityi

Most abundant in brain, and to a lesser extent in the spleen, the thymus and the liver. Also found in the T-cell lineage.

Gene expression databases

BgeeiP41242.
CleanExiMM_MATK.
ExpressionAtlasiP41242. baseline and differential.
GenevisibleiP41242. MM.

Interactioni

Subunit structurei

Interacts with KIT.By similarity

Protein-protein interaction databases

BioGridi201319. 1 interaction.
STRINGi10090.ENSMUSP00000113221.

Structurei

3D structure databases

ProteinModelPortaliP41242.
SMRiP41242. Positions 37-476.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini61 – 10848SH3PROSITE-ProRule annotationAdd
BLAST
Domaini120 – 20990SH2PROSITE-ProRule annotationAdd
BLAST
Domaini233 – 481249Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 SH2 domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH2 domain, SH3 domain

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233858.
HOVERGENiHBG008761.
InParanoidiP41242.
KOiK08888.
OrthoDBiEOG7SV0V5.
PhylomeDBiP41242.
TreeFamiTF351634.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00109. TYRKINASE.
SMARTiSM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P41242-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARRSSRVSW LAFEGWESRD LPRVSPRLFG AWHPAPAAAR MPTRWAPGTQ
60 70 80 90 100
CMTKCENSRP KPGELAFRKG DMVTILEACE DKSWYRAKHH GSGQEGLLAA
110 120 130 140 150
AALRHGEALS TDPKLSLMPW FHGKISGQEA IQQLQPPEDG LFLVRESARH
160 170 180 190 200
PGDYVLCVSF GRDVIHYRVL HRDGHLTIDE AVCFCNLMDM VEHYTKDKGA
210 220 230 240 250
ICTKLVKPRR KQGAKSAEEE LAKAGWLLDL QHLTLGAQIG EGEFGAVLQG
260 270 280 290 300
EYLGQKVAVK NIKCDVTAQA FLDETAVMTK LQHRNLVRLL GVILHHGLYI
310 320 330 340 350
VMEHVSKGNL VNFLRTRGRA LVSTSQLLQF ALHVAEGMEY LESKKLVHRD
360 370 380 390 400
LAARNILVSE DLVAKVSDFG LAKAERKGLD SSRLPVKWTA PEALKNGRFS
410 420 430 440 450
SKSDVWSFGV LLWEVFSYGR APYPKMSLKE VSEAVEKGYR MEPPDGCPGS
460 470 480 490 500
VHTLMGSCWE AEPARRPPFR KIVEKLGREL RSVGVSAPAG GQEAEGSAPT

RSQDP
Length:505
Mass (Da):56,056
Last modified:February 1, 1996 - v2
Checksum:i0A1661C1FBFD6A53
GO
Isoform 2 (identifier: P41242-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     43-43: T → TQ

Note: Minor isoform.
Show »
Length:506
Mass (Da):56,185
Checksum:iB77EF65C278EEB6E
GO
Isoform 3 (identifier: P41242-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-40: Missing.

Show »
Length:465
Mass (Da):51,495
Checksum:i6995C574F09FAE96
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti105 – 1062HG → QR (PubMed:8197166).Curated
Sequence conflicti105 – 1062HG → QR (PubMed:7898936).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 4040Missing in isoform 3. 2 PublicationsVSP_011565Add
BLAST
Alternative sequencei43 – 431T → TQ in isoform 2. CuratedVSP_004966

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U05210 mRNA. Translation: AAA18829.1.
L27738 mRNA. Translation: AAB59677.1.
D45243 mRNA. Translation: BAA08199.1.
L33339 Genomic DNA. Translation: AAA64431.1.
CCDSiCCDS24048.1. [P41242-1]
CCDS70078.1. [P41242-3]
PIRiI48926.
I59296.
RefSeqiNP_001272783.1. NM_001285854.1.
NP_001272784.1. NM_001285855.1.
NP_034898.1. NM_010768.2.
XP_006513358.1. XM_006513295.2.
XP_006513362.1. XM_006513299.1.
UniGeneiMm.2918.

Genome annotation databases

GeneIDi17179.
KEGGimmu:17179.
UCSCiuc007ggv.1. mouse. [P41242-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U05210 mRNA. Translation: AAA18829.1.
L27738 mRNA. Translation: AAB59677.1.
D45243 mRNA. Translation: BAA08199.1.
L33339 Genomic DNA. Translation: AAA64431.1.
CCDSiCCDS24048.1. [P41242-1]
CCDS70078.1. [P41242-3]
PIRiI48926.
I59296.
RefSeqiNP_001272783.1. NM_001285854.1.
NP_001272784.1. NM_001285855.1.
NP_034898.1. NM_010768.2.
XP_006513358.1. XM_006513295.2.
XP_006513362.1. XM_006513299.1.
UniGeneiMm.2918.

3D structure databases

ProteinModelPortaliP41242.
SMRiP41242. Positions 37-476.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201319. 1 interaction.
STRINGi10090.ENSMUSP00000113221.

PTM databases

PhosphoSiteiP41242.

Proteomic databases

MaxQBiP41242.
PaxDbiP41242.
PRIDEiP41242.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi17179.
KEGGimmu:17179.
UCSCiuc007ggv.1. mouse. [P41242-1]

Organism-specific databases

CTDi4145.
MGIiMGI:99259. Matk.

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233858.
HOVERGENiHBG008761.
InParanoidiP41242.
KOiK08888.
OrthoDBiEOG7SV0V5.
PhylomeDBiP41242.
TreeFamiTF351634.

Enzyme and pathway databases

BRENDAi2.7.10.2. 3474.
ReactomeiREACT_348099. Signaling by ERBB2.

Miscellaneous databases

NextBioi291490.
PROiP41242.
SOURCEiSearch...

Gene expression databases

BgeeiP41242.
CleanExiMM_MATK.
ExpressionAtlasiP41242. baseline and differential.
GenevisibleiP41242. MM.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00109. TYRKINASE.
SMARTiSM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Ctk: a protein-tyrosine kinase related to Csk that defines an enzyme family."
    Klages S., Adam D., Class K., Fargnoli J., Bolen J.B., Penhallow R.C.
    Proc. Natl. Acad. Sci. U.S.A. 91:2597-2601(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
    Tissue: Brain.
  2. "Ntk: a Csk-related protein-tyrosine kinase expressed in brain and T lymphocytes."
    Chow L.M.L., Jarvis C.D., Hu Q., Nye S.H., Gervais F.G., Veillette A., Matis L.A.
    Proc. Natl. Acad. Sci. U.S.A. 91:4975-4979(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: BALB/c.
    Tissue: Thymus.
  3. "Presence of alternative 5' untranslated sequences and identification of cells expressing ctk transcripts in the brain and testis."
    Kaneko Y., Nonoguchi K., Fukuyama H., Takano S., Higashitsuji H., Nishiyama H., Takenawa J., Nakayama H., Fujita J.
    Oncogene 10:945-952(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
    Strain: DDY/STD.
  4. "Two distinct protein isoforms are encoded by ntk, a csk-related tyrosine protein kinase gene."
    Chow L.M., Davidson D., Fournel M., Gosselin P., Lemieux S., Lyu M.S., Kozak C.A., Matis L.A., Veillette A.
    Oncogene 9:3437-3448(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-43 (ISOFORM 1), ALTERNATIVE SPLICING, SUBCELLULAR LOCATION.
    Strain: 129/Sv.
    Tissue: Kidney.

Entry informationi

Entry nameiMATK_MOUSE
AccessioniPrimary (citable) accession number: P41242
Secondary accession number(s): Q6LE90
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1996
Last modified: June 24, 2015
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.