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P41242 (MATK_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Megakaryocyte-associated tyrosine-protein kinase
EC=2.7.10.2
Alternative name(s):
Protein kinase NTK
Tyrosine-protein kinase CTK
Gene names
Name:Matk
Synonyms:Ctk, Ntk
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length505 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Could play a significant role in the signal transduction of hematopoietic cells. May regulate tyrosine kinase activity of SRC-family members in brain by specifically phosphorylating their C-terminal regulatory tyrosine residue which acts as a negative regulatory site. It may play an inhibitory role in the control of T-cell proliferation.

Catalytic activity

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subunit structure

Interacts with KIT By similarity.

Subcellular location

Cytoplasm. Membrane By similarity. Note: In platelets, 90% of MATK localizes to the membrane fraction, and translocates to the cytoskeleton upon thrombin stimulation By similarity. Ref.4

Tissue specificity

Most abundant in brain, and to a lesser extent in the spleen, the thymus and the liver. Also found in the T-cell lineage.

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.

Contains 1 protein kinase domain.

Contains 1 SH2 domain.

Contains 1 SH3 domain.

Ontologies

Keywords
   Cellular componentCytoplasm
Membrane
   Coding sequence diversityAlternative splicing
   DomainSH2 domain
SH3 domain
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
Tyrosine-protein kinase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from electronic annotation. Source: Compara

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

non-membrane spanning protein tyrosine kinase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P41242-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P41242-2)

The sequence of this isoform differs from the canonical sequence as follows:
     43-43: T → TQ
Note: Minor isoform.
Isoform 3 (identifier: P41242-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-40: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 505505Megakaryocyte-associated tyrosine-protein kinase
PRO_0000088074

Regions

Domain61 – 10848SH3
Domain120 – 20990SH2
Domain233 – 481249Protein kinase
Nucleotide binding239 – 2479ATP By similarity

Sites

Active site3501Proton acceptor By similarity
Binding site2601ATP By similarity

Natural variations

Alternative sequence1 – 4040Missing in isoform 3.
VSP_011565
Alternative sequence431T → TQ in isoform 2.
VSP_004966

Experimental info

Sequence conflict105 – 1062HG → QR Ref.2
Sequence conflict105 – 1062HG → QR Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: 0A1661C1FBFD6A53

FASTA50556,056
        10         20         30         40         50         60 
MARRSSRVSW LAFEGWESRD LPRVSPRLFG AWHPAPAAAR MPTRWAPGTQ CMTKCENSRP 

        70         80         90        100        110        120 
KPGELAFRKG DMVTILEACE DKSWYRAKHH GSGQEGLLAA AALRHGEALS TDPKLSLMPW 

       130        140        150        160        170        180 
FHGKISGQEA IQQLQPPEDG LFLVRESARH PGDYVLCVSF GRDVIHYRVL HRDGHLTIDE 

       190        200        210        220        230        240 
AVCFCNLMDM VEHYTKDKGA ICTKLVKPRR KQGAKSAEEE LAKAGWLLDL QHLTLGAQIG 

       250        260        270        280        290        300 
EGEFGAVLQG EYLGQKVAVK NIKCDVTAQA FLDETAVMTK LQHRNLVRLL GVILHHGLYI 

       310        320        330        340        350        360 
VMEHVSKGNL VNFLRTRGRA LVSTSQLLQF ALHVAEGMEY LESKKLVHRD LAARNILVSE 

       370        380        390        400        410        420 
DLVAKVSDFG LAKAERKGLD SSRLPVKWTA PEALKNGRFS SKSDVWSFGV LLWEVFSYGR 

       430        440        450        460        470        480 
APYPKMSLKE VSEAVEKGYR MEPPDGCPGS VHTLMGSCWE AEPARRPPFR KIVEKLGREL 

       490        500 
RSVGVSAPAG GQEAEGSAPT RSQDP

« Hide

Isoform 2 [UniParc].

Checksum: B77EF65C278EEB6E
Show »

FASTA50656,185
Isoform 3 [UniParc].

Checksum: 6995C574F09FAE96
Show »

FASTA46551,495

References

[1]"Ctk: a protein-tyrosine kinase related to Csk that defines an enzyme family."
Klages S., Adam D., Class K., Fargnoli J., Bolen J.B., Penhallow R.C.
Proc. Natl. Acad. Sci. U.S.A. 91:2597-2601(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
Tissue: Brain.
[2]"Ntk: a Csk-related protein-tyrosine kinase expressed in brain and T lymphocytes."
Chow L.M.L., Jarvis C.D., Hu Q., Nye S.H., Gervais F.G., Veillette A., Matis L.A.
Proc. Natl. Acad. Sci. U.S.A. 91:4975-4979(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Strain: BALB/c.
Tissue: Thymus.
[3]"Presence of alternative 5' untranslated sequences and identification of cells expressing ctk transcripts in the brain and testis."
Kaneko Y., Nonoguchi K., Fukuyama H., Takano S., Higashitsuji H., Nishiyama H., Takenawa J., Nakayama H., Fujita J.
Oncogene 10:945-952(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
Strain: DDY/STD.
[4]"Two distinct protein isoforms are encoded by ntk, a csk-related tyrosine protein kinase gene."
Chow L.M., Davidson D., Fournel M., Gosselin P., Lemieux S., Lyu M.S., Kozak C.A., Matis L.A., Veillette A.
Oncogene 9:3437-3448(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-43 (ISOFORM 1), ALTERNATIVE SPLICING, SUBCELLULAR LOCATION.
Strain: 129/Sv.
Tissue: Kidney.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U05210 mRNA. Translation: AAA18829.1.
L27738 mRNA. Translation: AAB59677.1.
D45243 mRNA. Translation: BAA08199.1.
L33339 Genomic DNA. Translation: AAA64431.1.
IPIIPI00271198.
IPI00461862.
IPI00990057.
PIRI48926.
I59296.
RefSeqNP_034898.1. NM_010768.1.
UniGeneMm.2918.

3D structure databases

ProteinModelPortalP41242.
SMRP41242. Positions 37-476.
ModBaseSearch...

PTM databases

PhosphoSiteP41242.

Proteomic databases

PaxDbP41242.
PRIDEP41242.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000105328; ENSMUSP00000100965; ENSMUSG00000004933.
ENSMUST00000117488; ENSMUSP00000113221; ENSMUSG00000004933.
ENSMUST00000119547; ENSMUSP00000113576; ENSMUSG00000004933.
GeneID17179.
KEGGmmu:17179.
UCSCuc007ggv.1. mouse.

Organism-specific databases

CTD4145.
MGIMGI:99259. Matk.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00600000084126.
HOGENOMHOG000233858.
HOVERGENHBG008761.
KOK08888.

Enzyme and pathway databases

BRENDA2.7.10.2. 3474.

Gene expression databases

ArrayExpressP41242.
BgeeP41242.
CleanExMM_MATK.
GenevestigatorP41242.
GermOnlineENSMUSG00000004933. Mus musculus.

Family and domain databases

Gene3D3.30.505.10. 1 hit.
InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_cat_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamPF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSPR00401. SH2DOMAIN.
PR00109. TYRKINASE.
SMARTSM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. Kinase_like. 1 hit.
SSF50044. SH3. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio291490.
SOURCESearch...

Entry information

Entry nameMATK_MOUSE
AccessionPrimary (citable) accession number: P41242
Secondary accession number(s): Q6LE90
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1996
Last modified: May 1, 2013
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

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