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P41242

- MATK_MOUSE

UniProt

P41242 - MATK_MOUSE

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Protein

Megakaryocyte-associated tyrosine-protein kinase

Gene

Matk

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Could play a significant role in the signal transduction of hematopoietic cells. May regulate tyrosine kinase activity of SRC-family members in brain by specifically phosphorylating their C-terminal regulatory tyrosine residue which acts as a negative regulatory site. It may play an inhibitory role in the control of T-cell proliferation.

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei260 – 2601ATPPROSITE-ProRule annotation
Active sitei350 – 3501Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi239 – 2479ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.2. 3474.
ReactomeiREACT_188191. Signaling by ERBB2.

Names & Taxonomyi

Protein namesi
Recommended name:
Megakaryocyte-associated tyrosine-protein kinase (EC:2.7.10.2)
Alternative name(s):
Protein kinase NTK
Tyrosine-protein kinase CTK
Gene namesi
Name:Matk
Synonyms:Ctk, Ntk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 10

Organism-specific databases

MGIiMGI:99259. Matk.

Subcellular locationi

Cytoplasm 1 Publication. Membrane By similarity
Note: In platelets, 90% of MATK localizes to the membrane fraction, and translocates to the cytoskeleton upon thrombin stimulation.By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 505505Megakaryocyte-associated tyrosine-protein kinasePRO_0000088074Add
BLAST

Proteomic databases

PaxDbiP41242.
PRIDEiP41242.

PTM databases

PhosphoSiteiP41242.

Expressioni

Tissue specificityi

Most abundant in brain, and to a lesser extent in the spleen, the thymus and the liver. Also found in the T-cell lineage.

Gene expression databases

BgeeiP41242.
CleanExiMM_MATK.
ExpressionAtlasiP41242. baseline and differential.
GenevestigatoriP41242.

Interactioni

Subunit structurei

Interacts with KIT.By similarity

Protein-protein interaction databases

BioGridi201319. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP41242.
SMRiP41242. Positions 37-476.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini61 – 10848SH3PROSITE-ProRule annotationAdd
BLAST
Domaini120 – 20990SH2PROSITE-ProRule annotationAdd
BLAST
Domaini233 – 481249Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 SH2 domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH2 domain, SH3 domain

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000119011.
HOGENOMiHOG000233858.
HOVERGENiHBG008761.
InParanoidiP41242.
KOiK08888.
OrthoDBiEOG7SV0V5.
PhylomeDBiP41242.
TreeFamiTF351634.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00109. TYRKINASE.
SMARTiSM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P41242-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MARRSSRVSW LAFEGWESRD LPRVSPRLFG AWHPAPAAAR MPTRWAPGTQ
60 70 80 90 100
CMTKCENSRP KPGELAFRKG DMVTILEACE DKSWYRAKHH GSGQEGLLAA
110 120 130 140 150
AALRHGEALS TDPKLSLMPW FHGKISGQEA IQQLQPPEDG LFLVRESARH
160 170 180 190 200
PGDYVLCVSF GRDVIHYRVL HRDGHLTIDE AVCFCNLMDM VEHYTKDKGA
210 220 230 240 250
ICTKLVKPRR KQGAKSAEEE LAKAGWLLDL QHLTLGAQIG EGEFGAVLQG
260 270 280 290 300
EYLGQKVAVK NIKCDVTAQA FLDETAVMTK LQHRNLVRLL GVILHHGLYI
310 320 330 340 350
VMEHVSKGNL VNFLRTRGRA LVSTSQLLQF ALHVAEGMEY LESKKLVHRD
360 370 380 390 400
LAARNILVSE DLVAKVSDFG LAKAERKGLD SSRLPVKWTA PEALKNGRFS
410 420 430 440 450
SKSDVWSFGV LLWEVFSYGR APYPKMSLKE VSEAVEKGYR MEPPDGCPGS
460 470 480 490 500
VHTLMGSCWE AEPARRPPFR KIVEKLGREL RSVGVSAPAG GQEAEGSAPT

RSQDP
Length:505
Mass (Da):56,056
Last modified:February 1, 1996 - v2
Checksum:i0A1661C1FBFD6A53
GO
Isoform 2 (identifier: P41242-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     43-43: T → TQ

Note: Minor isoform.

Show »
Length:506
Mass (Da):56,185
Checksum:iB77EF65C278EEB6E
GO
Isoform 3 (identifier: P41242-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-40: Missing.

Show »
Length:465
Mass (Da):51,495
Checksum:i6995C574F09FAE96
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti105 – 1062HG → QR(PubMed:8197166)Curated
Sequence conflicti105 – 1062HG → QR(PubMed:7898936)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 4040Missing in isoform 3. 2 PublicationsVSP_011565Add
BLAST
Alternative sequencei43 – 431T → TQ in isoform 2. CuratedVSP_004966

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U05210 mRNA. Translation: AAA18829.1.
L27738 mRNA. Translation: AAB59677.1.
D45243 mRNA. Translation: BAA08199.1.
L33339 Genomic DNA. Translation: AAA64431.1.
CCDSiCCDS24048.1. [P41242-1]
CCDS70078.1. [P41242-3]
PIRiI48926.
I59296.
RefSeqiNP_001272783.1. NM_001285854.1.
NP_001272784.1. NM_001285855.1.
NP_034898.1. NM_010768.2.
XP_006513358.1. XM_006513295.1.
XP_006513362.1. XM_006513299.1.
UniGeneiMm.2918.

Genome annotation databases

EnsembliENSMUST00000105328; ENSMUSP00000100965; ENSMUSG00000004933.
ENSMUST00000117488; ENSMUSP00000113221; ENSMUSG00000004933.
ENSMUST00000119547; ENSMUSP00000113576; ENSMUSG00000004933.
GeneIDi17179.
KEGGimmu:17179.
UCSCiuc007ggv.1. mouse. [P41242-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U05210 mRNA. Translation: AAA18829.1 .
L27738 mRNA. Translation: AAB59677.1 .
D45243 mRNA. Translation: BAA08199.1 .
L33339 Genomic DNA. Translation: AAA64431.1 .
CCDSi CCDS24048.1. [P41242-1 ]
CCDS70078.1. [P41242-3 ]
PIRi I48926.
I59296.
RefSeqi NP_001272783.1. NM_001285854.1.
NP_001272784.1. NM_001285855.1.
NP_034898.1. NM_010768.2.
XP_006513358.1. XM_006513295.1.
XP_006513362.1. XM_006513299.1.
UniGenei Mm.2918.

3D structure databases

ProteinModelPortali P41242.
SMRi P41242. Positions 37-476.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 201319. 1 interaction.

PTM databases

PhosphoSitei P41242.

Proteomic databases

PaxDbi P41242.
PRIDEi P41242.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000105328 ; ENSMUSP00000100965 ; ENSMUSG00000004933 .
ENSMUST00000117488 ; ENSMUSP00000113221 ; ENSMUSG00000004933 .
ENSMUST00000119547 ; ENSMUSP00000113576 ; ENSMUSG00000004933 .
GeneIDi 17179.
KEGGi mmu:17179.
UCSCi uc007ggv.1. mouse. [P41242-1 ]

Organism-specific databases

CTDi 4145.
MGIi MGI:99259. Matk.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00760000119011.
HOGENOMi HOG000233858.
HOVERGENi HBG008761.
InParanoidi P41242.
KOi K08888.
OrthoDBi EOG7SV0V5.
PhylomeDBi P41242.
TreeFami TF351634.

Enzyme and pathway databases

BRENDAi 2.7.10.2. 3474.
Reactomei REACT_188191. Signaling by ERBB2.

Miscellaneous databases

NextBioi 291490.
PROi P41242.
SOURCEi Search...

Gene expression databases

Bgeei P41242.
CleanExi MM_MATK.
ExpressionAtlasi P41242. baseline and differential.
Genevestigatori P41242.

Family and domain databases

Gene3Di 3.30.505.10. 1 hit.
InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view ]
Pfami PF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view ]
PRINTSi PR00401. SH2DOMAIN.
PR00109. TYRKINASE.
SMARTi SM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view ]
SUPFAMi SSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Ctk: a protein-tyrosine kinase related to Csk that defines an enzyme family."
    Klages S., Adam D., Class K., Fargnoli J., Bolen J.B., Penhallow R.C.
    Proc. Natl. Acad. Sci. U.S.A. 91:2597-2601(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
    Tissue: Brain.
  2. "Ntk: a Csk-related protein-tyrosine kinase expressed in brain and T lymphocytes."
    Chow L.M.L., Jarvis C.D., Hu Q., Nye S.H., Gervais F.G., Veillette A., Matis L.A.
    Proc. Natl. Acad. Sci. U.S.A. 91:4975-4979(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: BALB/c.
    Tissue: Thymus.
  3. "Presence of alternative 5' untranslated sequences and identification of cells expressing ctk transcripts in the brain and testis."
    Kaneko Y., Nonoguchi K., Fukuyama H., Takano S., Higashitsuji H., Nishiyama H., Takenawa J., Nakayama H., Fujita J.
    Oncogene 10:945-952(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3).
    Strain: DDY/STD.
  4. "Two distinct protein isoforms are encoded by ntk, a csk-related tyrosine protein kinase gene."
    Chow L.M., Davidson D., Fournel M., Gosselin P., Lemieux S., Lyu M.S., Kozak C.A., Matis L.A., Veillette A.
    Oncogene 9:3437-3448(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-43 (ISOFORM 1), ALTERNATIVE SPLICING, SUBCELLULAR LOCATION.
    Strain: 129/Sv.
    Tissue: Kidney.

Entry informationi

Entry nameiMATK_MOUSE
AccessioniPrimary (citable) accession number: P41242
Secondary accession number(s): Q6LE90
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1996
Last modified: October 29, 2014
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3