Reviewed,
UniProtKB/Swiss-Prot P41241 (CSK_MOUSE)
Last modified
February 9, 2010.
Version 104.
History...
Clusters with 100%,
90%,
50% identity |
Documents (4) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Tyrosine-protein kinase CSK EC=2.7.10.2 Alternative name(s): C-Src kinase Protein-tyrosine kinase MPK-2 p50CSK | ||
| Gene names |
| ||
| Organism | Mus musculus (Mouse) | ||
| Taxonomic identifier | 10090 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 450 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Specifically phosphorylates 'Tyr-504' on LCK, which acts as a negative regulatory site. Can also act on the LYN and FYN kinases. |
| Catalytic activity | ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. |
| Subunit structure | Interacts with phosphorylated SIT1 and LIME1 By similarity. Interacts with PTPN8. Interacts with phosphorylated PAG1 and TGFB1I1. Ref.4 Ref.5 Ref.6 Ref.7 Ref.8 |
| Subcellular location | Cytoplasm. Cell membrane. Note: Mainly cytoplasmic, also present in lipid rafts. Ref.8 |
| Tissue specificity | Ubiquitous, but most abundant in thymus and spleen, as well as in neonatal brain. |
| Sequence similarities | Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily. Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||||||||||||
Molecule processing | |||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 450 | 450 | Tyrosine-protein kinase CSK | PRO_0000088071 | |||||||||||||||
Regions | |||||||||||||||||||
| Domain | 9 – 70 | 62 | SH3 | ||||||||||||||||
| Domain | 82 – 171 | 90 | SH2 | ||||||||||||||||
| Domain | 195 – 449 | 255 | Protein kinase | ||||||||||||||||
| Nucleotide binding | 201 – 209 | 9 | ATP By similarity | ||||||||||||||||
| Region | 9 – 70 | 62 | Interaction with PTPN8 | ||||||||||||||||
Sites | |||||||||||||||||||
| Active site | 314 | 1 | Proton acceptor By similarity | ||||||||||||||||
| Binding site | 222 | 1 | ATP By similarity | ||||||||||||||||
Amino acid modifications | |||||||||||||||||||
| Modified residue | 184 | 1 | Phosphotyrosine By similarity | ||||||||||||||||
| Modified residue | 416 | 1 | Phosphotyrosine; by autocatalysis By similarity | ||||||||||||||||
Secondary structure | |||||||||||||||||||
Helix Strand Turn | |||||||||||||||||||
| Beta strand | 13 – 18 | 6 | |||||||||||||||||
| Beta strand | 35 – 41 | 7 | |||||||||||||||||
| Beta strand | 43 – 51 | 9 | |||||||||||||||||
| Beta strand | 57 – 61 | 5 | |||||||||||||||||
| Helix | 62 – 64 | 3 | |||||||||||||||||
| Beta strand | 65 – 67 | 3 | |||||||||||||||||
Sequences
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References
| [1] | "Ctk: a protein-tyrosine kinase related to Csk that defines an enzyme family." Klages S., Adam D., Class K., Fargnoli J., Bolen J.B., Penhallow R.C. Proc. Natl. Acad. Sci. U.S.A. 91:2597-2601(1994) [PubMed: 7511815] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain. |
| [2] | "An Eph-related receptor protein tyrosine kinase gene segmentally expressed in the developing mouse hindbrain." Gilardi-Hebenstreit P., Nieto M.A., Frain M., Mattei M.-G., Chestier A., Wilkinson D.G., Charnay P. Oncogene 7:2499-2506(1992) [PubMed: 1281307] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 316-367. Strain: C57BL/6. Tissue: Embryonic brain. |
| [3] | Lubec G., Sunyer B., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 338-347, MASS SPECTROMETRY. Strain: OF1. Tissue: Hippocampus. |
| [4] | "Association of inhibitory tyrosine protein kinase p50csk with protein tyrosine phosphatase PEP in T cells and other hemopoietic cells." Cloutier J.-F., Veillette A. EMBO J. 15:4909-4918(1996) [PubMed: 8890164] [Abstract] Cited for: INTERACTION WITH PTPN8. |
| [5] | "Characterization of a focal adhesion protein, Hic-5, that shares extensive homology with paxillin." Thomas S.M., Hagel M., Turner C.E. J. Cell Sci. 112:181-190(1999) [PubMed: 9858471] [Abstract] Cited for: INTERACTION WITH TGFB1I1. |
| [6] | "Fyn is essential for tyrosine phosphorylation of Csk-binding protein/phosphoprotein associated with glycolipid-enriched microdomains in lipid rafts in resting T cells." Yasuda K., Nagafuku M., Shima T., Okada M., Yagi T., Yamada T., Minaki Y., Kato A., Tani-Ichi S., Hamaoka T., Kosugi A. J. Immunol. 169:2813-2817(2002) [PubMed: 12218089] [Abstract] Cited for: INTERACTION WITH PAG1. |
| [7] | "Phosphorylation-dependent regulation of T-cell activation by PAG/Cbp, a lipid raft-associated transmembrane adaptor." Davidson D., Bakinowski M., Thomas M.L., Horejsi V., Veillette A. Mol. Cell. Biol. 23:2017-2028(2003) [PubMed: 12612075] [Abstract] Cited for: INTERACTION WITH PAG1. |
| [8] | "Cbp deficiency alters Csk localization in lipid rafts but does not affect T-cell development." Xu S., Huo J., Tan J.E.-L., Lam K.-P. Mol. Cell. Biol. 25:8486-8495(2005) [PubMed: 16166631] [Abstract] Cited for: INTERACTION WITH PAG1, SUBCELLULAR LOCATION. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| EMBL GenBank DDBJ | U05247 mRNA. Translation: AAA18766.1. X57242 mRNA. Translation: CAA40518.1. | ||||||||||||
| IPI | IPI00112648. | ||||||||||||
| PIR | I48929. | ||||||||||||
| UniGene | Mm.21974 Mm.470150 | ||||||||||||
3D structure databases | |||||||||||||
| PDBe RCSB PDB PDBj |
| ||||||||||||
| SMR | P41241. Positions 6-450. | ||||||||||||
| ModBase | Search... | ||||||||||||
Protein-protein interaction databases | |||||||||||||
| STRING | P41241. | ||||||||||||
PTM databases | |||||||||||||
| PhosphoSite | P41241. | ||||||||||||
Proteomic databases | |||||||||||||
| PRIDE | P41241. | ||||||||||||
Genome annotation databases | |||||||||||||
| Ensembl | ENSMUST00000034863; ENSMUSP00000034863; ENSMUSG00000032312; Mus musculus. [Genome view] | ||||||||||||
Organism-specific databases | |||||||||||||
| MGI | MGI:88537. Csk. | ||||||||||||
Phylogenomic databases | |||||||||||||
| eggNOG | roNOG05100. | ||||||||||||
| HOGENOM | HBG755340. | ||||||||||||
| HOVERGEN | P41241. | ||||||||||||
| InParanoid | P41241. | ||||||||||||
Enzyme and pathway databases | |||||||||||||
| BRENDA | 2.7.10.2. 244. | ||||||||||||
Gene expression databases | |||||||||||||
| ArrayExpress | P41241. | ||||||||||||
| Bgee | P41241. | ||||||||||||
| CleanEx | MM_CSK. | ||||||||||||
| Genevestigator | P41241. | ||||||||||||
| GermOnline | ENSMUSG00000032312. Mus musculus. | ||||||||||||
Family and domain databases | |||||||||||||
| InterPro | IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR000980. SH2. IPR001452. SH3_domain. IPR015778. Tyr_kinase_non-rcpt_Csk. IPR020635. Tyr_Pkinase_cat_dom. IPR020685. Tyr_prot_kinase. IPR008266. Tyr_prot_kinase_AS. [Graphical view] | ||||||||||||
| Gene3D | G3DSA:3.30.505.10. SH2. 1 hit. | ||||||||||||
| PANTHER | PTHR23256:SF264. Csk_ProtTyrKin. 1 hit. PTHR23256. Tyr_prot_kinase. 1 hit. | ||||||||||||
| Pfam | PF00017. SH2. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view] | ||||||||||||
| PRINTS | PR00401. SH2DOMAIN. | ||||||||||||
| SMART | SM00252. SH2. 1 hit. SM00326. SH3. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view] | ||||||||||||
| PROSITE | PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS50001. SH2. 1 hit. PS50002. SH3. 1 hit. [Graphical view] | ||||||||||||
| ProtoNet | Search... | ||||||||||||
Other Resources | |||||||||||||
| SOURCE | Search... | ||||||||||||
Entry information
| Entry name | CSK_MOUSE | ||||||||
| Accession | Primary (citable) accession number: P41241 Secondary accession number(s): Q03143 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| Human and mouse protein kinases Human and mouse protein kinases: classification and index |
| SIMILARITY comments Index of protein domains and families |

Clusters with


