P41239 (CSK_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tyrosine-protein kinase CSK
EC=2.7.10.2

Alternative name(s):
C-Src kinase

Gene names

Name:

CSK

Organism

Gallus gallus (Chicken) [Reference proteome]

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Sauropsida › Sauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs). Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane By similarity. Ref.2
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subunit structure	Homodimer (via SH3-domain) By similarity.
Subcellular location	Cytoplasm Probable.
Domain	The architecture of this protein is similar to that of Src-family kinases (SFKs) with one N-terminal SH3 domain, one SH2 domain, and a C-terminal kinase domain By similarity.
Post-translational modification	Phosphorylated at Ser-364 by PKA, leading to increased activity. Autophosphorylated By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily. Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain.

Ontologies

Keywords
Biological process	Adaptive immunity Immunity
Cellular component	Cytoplasm
Domain	SH2 domain SH3 domain
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 450	450	Tyrosine-protein kinase CSK		PRO_0000088069
Regions
	Domain	9 – 70	62	SH3
	Domain	82 – 171	90	SH2
	Domain	195 – 450	256	Protein kinase
	Nucleotide binding	201 – 209	9	ATP By similarity
	Region	9 – 70	62	Interaction with PTPN8 By similarity
Sites
	Active site	314	1	Proton acceptor By similarity
	Binding site	222	1	ATP By similarity
Amino acid modifications
	Modified residue	364	1	Phosphoserine; by PKA By similarity
	Modified residue	416	1	Phosphotyrosine

Sequences

	Sequence		Length	Mass (Da)
	P41239 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 5AA3C406AA4F246F	FASTA	450	50,751
	10 20 30 40 50 60 MSGMQAVWPS GTECIAKYNF HGTAEQDLPF SKGDVLTIVA VTKDPNWYKA KNKVGREGII 70 80 90 100 110 120 PANYVQKREG VKAGIKLSLM PWFHGKITRE QAERLLYPPE TGLFLVREST NYPGDYTLCV 130 140 150 160 170 180 SCEGKVEHYR IIYSSSKLSI DEEVYFENLM QLVEHYTTDA DGLCSRLIKP KVMEGTVAAQ 190 200 210 220 230 240 DEFSRSGWAL NMKDLKLLQI IGKGEFGDVM LGDYRGNKVA VKCIKNDATA QAFLAEASVM 250 260 270 280 290 300 TQLRHSNLVQ LLGVIVEEKS GLYIVTEYMA KGSLVDYLRS RGRSVLGGDC LLKFSLDVCE 310 320 330 340 350 360 AMEYLEANNF VHRDLAARNV LVSEDNIAKV SDFGLTKEAS STQDTGKLPV KWTAPEALRE 370 380 390 400 410 420 KKFSTKSDVW SFGILLWEIY SFGRVPYPRI PLKDVVPRVE KGYKMDPPDG CPAIVYEVMK 430 440 450 KCWTLDPGHR PSFHQLREQL VHIKEKELYL

References

[1]	"Molecular cloning and expression of chicken C-terminal Src kinase: lack of stable association with c-Src protein." Sabe H., Knudsen B., Okada M., Nada S., Nakagawa H., Hanafusa H. Proc. Natl. Acad. Sci. U.S.A. 89:2190-2194(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Functional analysis of Csk in signal transduction through the B-cell antigen receptor." Hata A., Sabe H., Kurosaki T., Takata M., Hanafusa H. Mol. Cell. Biol. 14:7306-7313(1994) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M85039 mRNA. Translation: AAA51436.1.
IPI	IPI00597367.
PIR	A41973.
RefSeq	NP_990756.1. NM_205425.1.
UniGene	Gga.2691.
3D structure databases
ProteinModelPortal	P41239.
SMR	P41239. Positions 6-450.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-139440.
Proteomic databases
PRIDE	P41239.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	396396.
KEGG	gga:396396.
Organism-specific databases
CTD	1445.
Phylogenomic databases
HOVERGEN	HBG008761.
InParanoid	P41239.
KO	K05728.
Enzyme and pathway databases
BRENDA	2.7.10.2. 1306.
Family and domain databases
Gene3D	3.30.505.10. 1 hit.
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR001245. Ser-Thr/Tyr_kinase_cat_dom. IPR000980. SH2. IPR001452. SH3_domain. IPR008266. Tyr_kinase_AS. IPR020635. Tyr_kinase_cat_dom. [Graphical view]
Pfam	PF07714. Pkinase_Tyr. 1 hit. PF00017. SH2. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view]
PRINTS	PR00401. SH2DOMAIN. PR00109. TYRKINASE.
SMART	SM00252. SH2. 1 hit. SM00326. SH3. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit. SSF50044. SH3. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS50001. SH2. 1 hit. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20816438.

Entry information

Entry name

CSK_CHICK

Accession

Primary (citable) accession number: P41239

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	April 3, 2013
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program