Reviewed, UniProtKB/Swiss-Prot P41239 (CSK_CHICK)

Names and origin

Protein names

Recommended name:
    Tyrosine-protein kinase CSK
    EC=2.7.10.2
Alternative name(s):
    C-SRC kinase

Gene names

Name:

CSK

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	450 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Specifically phosphorylates a tyrosine on the SRC kinase. This tyrosine acts as a negative regulatory site. Can also act on the LYN and FYN kinases.
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subunit structure	Interacts with PTPN8 By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily. Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Domain	SH2 domain SH3 domain
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 450	450	Tyrosine-protein kinase CSK		PRO_0000088069
Regions
	Domain	9 – 70	62	SH3
	Domain	82 – 171	90	SH2
	Domain	195 – 450	256	Protein kinase
	Nucleotide binding	201 – 209	9	ATP By similarity
	Region	9 – 70	62	Interaction with PTPN8 By similarity
Sites
	Active site	314	1	Proton acceptor By similarity
	Binding site	222	1	ATP By similarity
Amino acid modifications
	Modified residue	416	1	Phosphotyrosine; by autocatalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	P41239-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 5AA3C406AA4F246F	FASTA	450	50,751
	10 20 30 40 50 60 MSGMQAVWPS GTECIAKYNF HGTAEQDLPF SKGDVLTIVA VTKDPNWYKA KNKVGREGII 70 80 90 100 110 120 PANYVQKREG VKAGIKLSLM PWFHGKITRE QAERLLYPPE TGLFLVREST NYPGDYTLCV 130 140 150 160 170 180 SCEGKVEHYR IIYSSSKLSI DEEVYFENLM QLVEHYTTDA DGLCSRLIKP KVMEGTVAAQ 190 200 210 220 230 240 DEFSRSGWAL NMKDLKLLQI IGKGEFGDVM LGDYRGNKVA VKCIKNDATA QAFLAEASVM 250 260 270 280 290 300 TQLRHSNLVQ LLGVIVEEKS GLYIVTEYMA KGSLVDYLRS RGRSVLGGDC LLKFSLDVCE 310 320 330 340 350 360 AMEYLEANNF VHRDLAARNV LVSEDNIAKV SDFGLTKEAS STQDTGKLPV KWTAPEALRE 370 380 390 400 410 420 KKFSTKSDVW SFGILLWEIY SFGRVPYPRI PLKDVVPRVE KGYKMDPPDG CPAIVYEVMK 430 440 450 KCWTLDPGHR PSFHQLREQL VHIKEKELYL

References

[1]

"Molecular cloning and expression of chicken C-terminal Src kinase: lack of stable association with c-Src protein." Sabe H., Knudsen B., Okada M., Nada S., Nakagawa H., Hanafusa H. Proc. Natl. Acad. Sci. U.S.A. 89:2190-2194(1992) [PubMed: 1372437] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M85039 mRNA. Translation: AAA51436.1.
IPI	IPI00597367.
PIR	A41973.
RefSeq	NP_990756.1.
UniGene	Gga.2691
3D structure databases
HSSP	HSSP built from PDB template 1BYG based on UniProtKB P41240.
SMR	P41239. Positions 4-450.
ModBase	Search...
Proteomic databases
PRIDE	P41239.
Genome annotation databases
Ensembl	ENSGALG00000001318. Gallus gallus. [Contig view]
GeneID	396396.
KEGG	gga:396396.
Phylogenomic databases
HOGENOM	P41239.
HOVERGEN	P41239.
Enzyme and pathway databases
BRENDA	2.7.10.2. 4.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR000980. SH2. IPR001452. SH3_domain. IPR001245. Tyr_pkinase. IPR008266. Tyr_pkinase_AS. IPR015778. Tyrosine_Protein_Kinase_Csk. [Graphical view]
Gene3D	G3DSA:3.30.505.10. SH2. 1 hit.
PANTHER	PTHR23256:SF264. Csk_ProtTyrKin. 1 hit.
Pfam	PF07714. Pkinase_Tyr. 1 hit. PF00017. SH2. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view]
PRINTS	PR00401. SH2DOMAIN. PR00109. TYRKINASE.
ProDom	PD000001. Prot_kinase. 1 hit. PD000093. SH2. 1 hit. PD000066. SH3. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00252. SH2. 1 hit. SM00326. SH3. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS50001. SH2. 1 hit. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CSK_CHICK

Accession

Primary (citable) accession number: P41239

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	June 16, 2009
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)