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P41239 (CSK_CHICK) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tyrosine-protein kinase CSK

EC=2.7.10.2
Alternative name(s):
C-Src kinase
Gene names
Name:CSK
OrganismGallus gallus (Chicken) [Reference proteome]
Taxonomic identifier9031 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiTestudines + Archosauria groupArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalliformesPhasianidaePhasianinaeGallus

Protein attributes

Sequence length450 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs). Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane By similarity. Ref.2

Catalytic activity

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subunit structure

Homodimer (via SH3-domain) By similarity.

Subcellular location

Cytoplasm Probable.

Domain

The architecture of this protein is similar to that of Src-family kinases (SFKs) with one N-terminal SH3 domain, one SH2 domain, and a C-terminal kinase domain By similarity.

Post-translational modification

Phosphorylated at Ser-364 by PKA, leading to increased activity. Autophosphorylated By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. CSK subfamily.

Contains 1 protein kinase domain.

Contains 1 SH2 domain.

Contains 1 SH3 domain.

Ontologies

Keywords
   Biological processAdaptive immunity
Immunity
   Cellular componentCytoplasm
   DomainSH2 domain
SH3 domain
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
Tyrosine-protein kinase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processimmune system process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

non-membrane spanning protein tyrosine kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 450450Tyrosine-protein kinase CSK
PRO_0000088069

Regions

Domain9 – 7062SH3
Domain82 – 17190SH2
Domain195 – 450256Protein kinase
Nucleotide binding201 – 2099ATP By similarity
Region9 – 7062Interaction with PTPN8 By similarity

Sites

Active site3141Proton acceptor By similarity
Binding site2221ATP By similarity

Amino acid modifications

Modified residue3641Phosphoserine; by PKA By similarity
Modified residue4161Phosphotyrosine

Sequences

Sequence LengthMass (Da)Tools
P41239 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 5AA3C406AA4F246F

FASTA45050,751
        10         20         30         40         50         60 
MSGMQAVWPS GTECIAKYNF HGTAEQDLPF SKGDVLTIVA VTKDPNWYKA KNKVGREGII 

        70         80         90        100        110        120 
PANYVQKREG VKAGIKLSLM PWFHGKITRE QAERLLYPPE TGLFLVREST NYPGDYTLCV 

       130        140        150        160        170        180 
SCEGKVEHYR IIYSSSKLSI DEEVYFENLM QLVEHYTTDA DGLCSRLIKP KVMEGTVAAQ 

       190        200        210        220        230        240 
DEFSRSGWAL NMKDLKLLQI IGKGEFGDVM LGDYRGNKVA VKCIKNDATA QAFLAEASVM 

       250        260        270        280        290        300 
TQLRHSNLVQ LLGVIVEEKS GLYIVTEYMA KGSLVDYLRS RGRSVLGGDC LLKFSLDVCE 

       310        320        330        340        350        360 
AMEYLEANNF VHRDLAARNV LVSEDNIAKV SDFGLTKEAS STQDTGKLPV KWTAPEALRE 

       370        380        390        400        410        420 
KKFSTKSDVW SFGILLWEIY SFGRVPYPRI PLKDVVPRVE KGYKMDPPDG CPAIVYEVMK 

       430        440        450 
KCWTLDPGHR PSFHQLREQL VHIKEKELYL 

« Hide

References

[1]"Molecular cloning and expression of chicken C-terminal Src kinase: lack of stable association with c-Src protein."
Sabe H., Knudsen B., Okada M., Nada S., Nakagawa H., Hanafusa H.
Proc. Natl. Acad. Sci. U.S.A. 89:2190-2194(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"Functional analysis of Csk in signal transduction through the B-cell antigen receptor."
Hata A., Sabe H., Kurosaki T., Takata M., Hanafusa H.
Mol. Cell. Biol. 14:7306-7313(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M85039 mRNA. Translation: AAA51436.1.
PIRA41973.
RefSeqNP_990756.1. NM_205425.1.
UniGeneGga.2691.

3D structure databases

ProteinModelPortalP41239.
SMRP41239. Positions 6-450.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid676646. 1 interaction.
MINTMINT-139440.

Proteomic databases

PRIDEP41239.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID396396.
KEGGgga:396396.

Organism-specific databases

CTD1445.

Phylogenomic databases

HOVERGENHBG008761.
InParanoidP41239.
KOK05728.
PhylomeDBP41239.

Enzyme and pathway databases

BRENDA2.7.10.2. 1306.

Family and domain databases

Gene3D3.30.505.10. 1 hit.
InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamPF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSPR00401. SH2DOMAIN.
PR00109. TYRKINASE.
SMARTSM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMSSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20816438.
PROP41239.

Entry information

Entry nameCSK_CHICK
AccessionPrimary (citable) accession number: P41239
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 16, 2014
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families