P41236 (IPP2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein phosphatase inhibitor 2
Short name=IPP-2

Gene names

Name:	PPP1R2
Synonyms:	IPP2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	205 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibitor of protein-phosphatase 1.
Subunit structure	Heterodimer with PP1. Ref.9
Post-translational modification	Phosphorylation on Thr-73 by GSK3 activates PP1 by dissociating the PP1-PPP1R2 complex By similarity. Phosphorylation on Ser-44 by ATM activates PP1 by dissociating the PP1-PPP1R2 complex.
Sequence similarities	Belongs to the protein phosphatase inhibitor 2 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen metabolism
Molecular function	Protein phosphatase inhibitor
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	generation of precursor metabolites and energy Traceable author statement Ref.3. Source: ProtInc glycogen metabolic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of phosphoprotein phosphatase activity Inferred from electronic annotation. Source: InterPro regulation of signal transduction Inferred from electronic annotation. Source: InterPro
Molecular_function	protein serine/threonine phosphatase inhibitor activity Traceable author statement Ref.2. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
LMTK2	Q8IWU2	3	EBI-1056517,EBI-2008933

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.7
	Chain	2 – 205	204	Protein phosphatase inhibitor 2		PRO_0000071481
Regions
	Region	12 – 17	6	Required for binding PPP1CC By similarity
	Region	43 – 55	13	Required for binding PPP1CC By similarity
	Region	147 – 150	4	Required for binding PPP1CC catalytic center, displacing metal ions and inhibition of PPP1CC catyltic activity By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	44	1	Phosphoserine; by ATM Ref.9
	Modified residue	73	1	Phosphothreonine; by GSK3 By similarity
	Modified residue	87	1	Phosphoserine Ref.10 Ref.11
	Modified residue	89	1	Phosphothreonine Ref.10
	Modified residue	92	1	Phosphothreonine Ref.8
	Modified residue	121	1	Phosphoserine By similarity
	Modified residue	122	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P41236 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: FFC005B07CD5ABFC	FASTA	205	23,015
	10 20 30 40 50 60 MAASTASHRP IKGILKNKTS TTSSMVASAE QPRGNVDEEL SKKSQKWDEM NILATYHPAD 70 80 90 100 110 120 KDYGLMKIDE PSTPYHSMMG DDEDACSDTE ATEAMAPDIL ARKLAAAEGL EPKYRIQEQE 130 140 150 160 170 180 SSGEEDSDLS PEEREKKRQF EMKRKLHYNE GLNIKLARQL ISKDLHDDDE DEEMLETADG 190 200 ESMNTEESNQ GSTPSDQQQN KLRSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of human phosphatase inhibitor-2 (IPP-2) sequences." Sanseau P., Jackson A., Alderton R.P., Beck S., Senger G., Sheer D., Kelly A., Trowsdale J. Mamm. Genome 5:490-496(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cloning of the complete coding region for human protein phosphatase inhibitor 2 using the two hybrid system and expression of inhibitor 2 in E. coli." Helps N.R., Street A.J., Elledge S.J., Cohen P.T.W. FEBS Lett. 340:93-98(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Genetic analysis of human type 1 protein phosphatase inhibitor 2 in insulin-resistant Pima Indians." Permana P.A., Mott D.M. Genomics 41:110-114(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Inhibitor 2 of protein phosphatase 1 is differentially expressed between the colon cancer cell lines SW480 and SW620." Croke D.T., McWilliam P.M., Parle-McDermott A., Dunican D., Tighe O. Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]	"Cloning of inhibitor-2 of protein phosphatase type 1 from human heart." Mishra S., Tiwari N., Sabbah H.N., Gupta R.C. Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Heart.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skin.
[7]	"Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J. Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-33. Tissue: Platelet.
[8]	"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis." Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-92, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[9]	"A novel ATM-dependent pathway regulates protein phosphatase 1 in response to DNA damage." Tang X., Hui Z.G., Cui X.L., Garg R., Kastan M.B., Xu B. Mol. Cell. Biol. 28:2559-2566(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION AT SER-44, SUBUNIT.
[10]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-87 AND THR-89, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-87, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
[12]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X78873 mRNA. Translation: CAA55475.1. Z29646 mRNA. Translation: CAA82754.1. U68111 , U68106, U68107, U68108, U68109, U68110 Genomic DNA. Translation: AAC51206.1. AJ133812 mRNA. Translation: CAB41680.1. AY063767 mRNA. Translation: AAL48322.1. BC007655 mRNA. Translation: AAH07655.1.
IPI	IPI00220402.
PIR	S42406.
RefSeq	NP_006232.1. NM_006241.4.
UniGene	Hs.535731. Hs.706920.
3D structure databases
ProteinModelPortal	P41236.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-781N.
IntAct	P41236. 8 interactions.
STRING	9606.ENSP00000328178.
PTM databases
PhosphoSite	P41236.
Polymorphism databases
DMDM	729856.
Proteomic databases
PRIDE	P41236.
Protocols and materials databases
DNASU	5504.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000328432; ENSP00000328178; ENSG00000184203.
GeneID	5504.
KEGG	hsa:5504.
UCSC	uc003fup.3. human.
Organism-specific databases
CTD	5504.
GeneCards	GC03M195241.
H-InvDB	HIX0032078. HIX0166147. HIX0166426. HIX0166678. HIX0167196. HIX0200910.
HGNC	HGNC:9288. PPP1R2.
HPA	CAB025582.
MIM	601792. gene.
neXtProt	NX_P41236.
PharmGKB	PA33641.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	HBG006170.
InParanoid	P41236.
KO	K16833.
OMA	KIDEPNT.
PhylomeDB	P41236.
Gene expression databases
ArrayExpress	P41236.
Bgee	P41236.
CleanEx	HS_PPP1R2.
Genevestigator	P41236.
GermOnline	ENSG00000184203. Homo sapiens.
Family and domain databases
InterPro	IPR007062. PPI-2. [Graphical view]
Pfam	PF04979. IPP-2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	5504.
NextBio	21294.
SOURCE	Search...

Entry information

Entry name

IPP2_HUMAN

Accession

Primary (citable) accession number: P41236

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 123 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.