P41231 (P2RY2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
P2Y purinoceptor 2
Short name=P2Y2

Alternative name(s):
ATP receptor
P2U purinoceptor 1
Short name=P2U1
Purinergic receptor

Gene names

Name:	P2RY2
Synonyms:	P2RU1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	377 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for ATP and UTP coupled to G-proteins that activate a phosphatidylinositol-calcium second messenger system. The affinity range is UTP = ATP > ATP-gamma-S >> 2-methylthio-ATP = ADP.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Spleen, testis, kidney, liver, lung, heart and brain.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular ion homeostasis Traceable author statement Ref.1. Source: ProtInc phospholipase C-activating G-protein coupled receptor signaling pathway Traceable author statement Ref.1. Source: ProtInc positive regulation of mucus secretion Inferred from electronic annotation. Source: Compara
Cellular_component	integral to plasma membrane Traceable author statement Ref.1. Source: ProtInc
Molecular_function	G-protein coupled purinergic nucleotide receptor activity Inferred from electronic annotation. Source: InterPro receptor activity Traceable author statement PubMed 7779087. Source: ProtInc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 377	377	P2Y purinoceptor 2		PRO_0000070013
Regions
	Topological domain	1 – 32	32	Extracellular Potential
	Transmembrane	33 – 59	27	Helical; Name=1; Potential
	Topological domain	60 – 70	11	Cytoplasmic Potential
	Transmembrane	71 – 93	23	Helical; Name=2; Potential
	Topological domain	94 – 110	17	Extracellular Potential
	Transmembrane	111 – 129	19	Helical; Name=3; Potential
	Topological domain	130 – 152	23	Cytoplasmic Potential
	Transmembrane	153 – 172	20	Helical; Name=4; Potential
	Topological domain	173 – 194	22	Extracellular Potential
	Transmembrane	195 – 220	26	Helical; Name=5; Potential
	Topological domain	221 – 246	26	Cytoplasmic Potential
	Transmembrane	247 – 269	23	Helical; Name=6; Potential
	Topological domain	270 – 287	18	Extracellular Potential
	Transmembrane	288 – 309	22	Helical; Name=7; Potential
	Topological domain	310 – 377	68	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	9	1	N-linked (GlcNAc...) Potential
	Glycosylation	13	1	N-linked (GlcNAc...) Potential
	Disulfide bond	106 ↔ 183		By similarity
Natural variations
	Natural variant	46	1	P → L. Ref.1 Ref.3 Ref.4 Ref.6 Ref.7 Corresponds to variant rs2511241 [ dbSNP | Ensembl ].		VAR_054870
	Natural variant	312	1	R → S. Ref.7 Corresponds to variant rs3741156 [ dbSNP | Ensembl ].		VAR_054871
	Natural variant	334	1	R → C. Ref.4 Ref.6 Corresponds to variant rs1626154 [ dbSNP | Ensembl ].		VAR_054872
Experimental info
	Sequence conflict	350	1	E → G in AAC04923. Ref.1
	Sequence conflict	359	1	S → F in AAC04923. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P41231 [UniParc]. Last modified November 2, 2010. Version 4. Checksum: F71DAF4A8DCB4062	FASTA	377	42,273
	10 20 30 40 50 60 MAADLGPWND TINGTWDGDE LGYRCRFNED FKYVLLPVSY GVVCVPGLCL NAVALYIFLC 70 80 90 100 110 120 RLKTWNASTT YMFHLAVSDA LYAASLPLLV YYYARGDHWP FSTVLCKLVR FLFYTNLYCS 130 140 150 160 170 180 ILFLTCISVH RCLGVLRPLR SLRWGRARYA RRVAGAVWVL VLACQAPVLY FVTTSARGGR 190 200 210 220 230 240 VTCHDTSAPE LFSRFVAYSS VMLGLLFAVP FAVILVCYVL MARRLLKPAY GTSGGLPRAK 250 260 270 280 290 300 RKSVRTIAVV LAVFALCFLP FHVTRTLYYS FRSLDLSCHT LNAINMAYKV TRPLASANSC 310 320 330 340 350 360 LDPVLYFLAG QRLVRFARDA KPPTGPSPAT PARRRLGLRR SDRTDMQRIE DVLGSSEDSR 370 RTESTPAGSE NTKDIRL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and expression of a human P2U nucleotide receptor, a target for cystic fibrosis pharmacotherapy." Parr C.E., Sullivan D.M., Paradiso A.M., Lazarowski E.R., Burch L.H., Olsen J.C., Erb L., Weisman G.A., Boucher R.C., Turner J.T. Proc. Natl. Acad. Sci. U.S.A. 91:3275-3279(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT LEU-46. Tissue: Airway epithelium.
[2]	"Cloning and expression of a human P2U nucleotide receptor, a target for cystic fibrosis pharmacotherapy." Parr C.E., Sullivan D.M., Paradiso A.M., Lazarowski E.R., Burch L.H., Olsen J.C., Erb L., Weisman G.A., Boucher R.C., Turner J.T. Proc. Natl. Acad. Sci. U.S.A. 91:13067-13067(1994) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION.
[3]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT LEU-46. Tissue: Placenta.
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS LEU-46 AND CYS-334. Tissue: Pericardium.
[5]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANTS LEU-46 AND CYS-334.
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANTS LEU-46 AND SER-312. Tissue: Kidney and Leukocyte.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U07225 mRNA. Translation: AAC04923.1.
AY136753 mRNA. Translation: AAN01279.1.
AK313942 mRNA. Translation: BAG36660.1.
AP002761 Genomic DNA. No translation available.
CH471076 Genomic DNA. Translation: EAW74882.1.
BC012104 mRNA. Translation: AAH12104.1.
BC028135 mRNA. Translation: AAH28135.1.

IPI

IPI00298653.

PIR

A54946.

RefSeq

NP_002555.2. NM_002564.2.
NP_788085.1. NM_176071.1.
NP_788086.1. NM_176072.1.

UniGene

Hs.339.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1Z8E	model	-	A	1-377	[»]

ProteinModelPortal

P41231.

SMR

P41231. Positions 27-325.

ModBase

Search...

Protein-protein interaction databases

STRING

9606.ENSP00000310305.

Protein family/group databases

GPCRDB

Search...

PTM databases

PhosphoSite

P41231.

Polymorphism databases

DMDM

311033490.

Proteomic databases

PaxDb

P41231.

PRIDE

P41231.

Protocols and materials databases

DNASU

5029.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000311131; ENSP00000310305; ENSG00000175591.
ENST00000393596; ENSP00000377221; ENSG00000175591.
ENST00000393597; ENSP00000377222; ENSG00000175591.

GeneID

5029.

KEGG

hsa:5029.

UCSC

uc001otj.3. human.

Organism-specific databases

CTD

5029.

GeneCards

GC11P072929.

H-InvDB

HIX0009916.

HGNC

HGNC:8541. P2RY2.

HPA

CAB022642.

MIM

600041. gene.

neXtProt

NX_P41231.

PharmGKB

PA32870.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG147537.

HOGENOM

HOG000231307.

HOVERGEN

HBG101120.

InParanoid

P41231.

KO

K04269.

OMA

RDAKPPT.

OrthoDB

EOG44J2JK.

PhylomeDB

P41231.

Enzyme and pathway databases

Reactome

REACT_111102. Signal Transduction.

Gene expression databases

Bgee

P41231.

CleanEx

HS_P2RY2.

Genevestigator

P41231.

GermOnline

ENSG00000175591. Homo sapiens.

Family and domain databases

InterPro

IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR002286. P2_purnocptor.
IPR000356. P2U_purnocptor.
[Graphical view]

Pfam

PF00001. 7tm_1. 1 hit.
[Graphical view]

PRINTS

PR00237. GPCRRHODOPSN.
PR00594. P2Y2PRNOCPTR.
PR01157. P2YPURNOCPTR.

PROSITE

PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

P41231.

ChEMBL

CHEMBL4398.

DrugBank

DB04786. Suramin.

GenomeRNAi

5029.

NextBio

19366.

SOURCE

Search...

Entry information

Entry name

P2RY2_HUMAN

Accession

Primary (citable) accession number: P41231
Secondary accession number(s): B2R9W3, Q96EM8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	November 2, 2010
Last modified:	May 1, 2013
This is version 123 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.