P41226 (UBA7_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-like modifier-activating enzyme 7
Short name=Ubiquitin-activating enzyme 7

Alternative name(s):
D8
Ubiquitin-activating enzyme E1 homolog

Gene names

Name:	UBA7
Synonyms:	UBE1L, UBE2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1012 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Activates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP. Ref.8
Pathway	Protein modification; protein ubiquitination. Ref.7
Subunit structure	Monomer By similarity. Binds and is involved in the conjugation of G1P2/ISG15.
Tissue specificity	Expressed in a variety of normal and tumor cell types, but is reduced in lung cancer cell lines.
Miscellaneous	There are two active sites within the E1 molecule, allowing it to accommodate two ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site.
Sequence similarities	Belongs to the ubiquitin-activating E1 family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Coding sequence diversity	Polymorphism
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	ISG15-protein conjugation Inferred from direct assay PubMed 16428300. Source: HGNC cytokine-mediated signaling pathway Traceable author statement. Source: Reactome innate immune response Traceable author statement. Source: Reactome modification-dependent protein catabolic process Inferred from electronic annotation. Source: Compara negative regulation of type I interferon production Traceable author statement. Source: Reactome protein ubiquitination Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytosol Traceable author statement. Source: Reactome
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ISG15 activating enzyme activity Inferred from direct assay PubMed 16428300. Source: HGNC ligase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1012	1012	Ubiquitin-like modifier-activating enzyme 7		PRO_0000194937
Regions
	Repeat	23 – 159	137	1-1
	Repeat	423 – 575	153	1-2
	Nucleotide binding	442 – 471	30	ATP By similarity
	Region	23 – 575	553	2 approximate repeats
Sites
	Active site	599	1	Glycyl thioester intermediate By similarity
Natural variations
	Natural variant	712	1	P → S. Corresponds to variant rs11928913 [ dbSNP | Ensembl ].		VAR_052434
	Natural variant	817	1	H → R. Corresponds to variant rs2230149 [ dbSNP | Ensembl ].		VAR_047793
Experimental info
	Sequence conflict	355 – 378	24	GVLSP…KAISR → RCLEPMVACWVSSCPGSAEG NLQ in AAA75388. Ref.1
	Sequence conflict	355 – 378	24	GVLSP…KAISR → RCLEPMVACWVSSCPGSAEG NLQ in AAG49557. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P41226 [UniParc]. Last modified November 25, 2008. Version 2. Checksum: A01E1106D81778EB	FASTA	1,012	111,694
	10 20 30 40 50 60 MDALDASKLL DEELYSRQLY VLGSPAMQRI QGARVLVSGL QGLGAEVAKN LVLMGVGSLT 70 80 90 100 110 120 LHDPHPTCWS DLAAQFLLSE QDLERSRAEA SQELLAQLNR AVQVVVHTGD ITEDLLLDFQ 130 140 150 160 170 180 VVVLTAAKLE EQLKVGTLCH KHGVCFLAAD TRGLVGQLFC DFGEDFTVQD PTEAEPLTAA 190 200 210 220 230 240 IQHISQGSPG ILTLRKGANT HYFRDGDLVT FSGIEGMVEL NDCDPRSIHV REDGSLEIGD 250 260 270 280 290 300 TTTFSRYLRG GAITEVKRPK TVRHKSLDTA LLQPHVVAQS SQEVHHAHCL HQAFCALHKF 310 320 330 340 350 360 QHLHGRPPQP WDPVDAETVV GLARDLEPLK RTEEEPLEEP LDEALVRTVA LSSAGVLSPM 370 380 390 400 410 420 VAMLGAVAAQ EVLKAISRKF MPLDQWLYFD ALDCLPEDGE LLPSPEDCAL RGSRYDGQIA 430 440 450 460 470 480 VFGAGFQEKL RRQHYLLVGA GAIGCELLKV FALVGLGAGN SGGLTVVDMD HIERSNLSRQ 490 500 510 520 530 540 FLFRSQDVGR PKAEVAAAAA RGLNPDLQVI PLTYPLDPTT EHIYGDNFFS RVDGVAAALD 550 560 570 580 590 600 SFQARRYVAA RCTHYLKPLL EAGTSGTWGS ATVFMPHVTE AYRAPASAAA SEDAPYPVCT 610 620 630 640 650 660 VRYFPSTAEH TLQWARHEFE ELFRLSAETI NHHQQAHTSL ADMDEPQTLT LLKPVLGVLR 670 680 690 700 710 720 VRPQNWQDCV AWALGHWKLC FHYGIKQLLR HFPPNKVLED GTPFWSGPKQ CPQPLEFDTN 730 740 750 760 770 780 QDTHLLYVLA AANLYAQMHG LPGSQDWTAL RELLKLLPQP DPQQMAPIFA SNLELASASA 790 800 810 820 830 840 EFGPEQQKEL NKALEVWSVG PPLKPLMFEK DDDSNFHVDF VVAAASLRCQ NYGIPPVNRA 850 860 870 880 890 900 QSKRIVGQII PAIATTTAAV AGLLGLELYK VVSGPRPRSA FRHSYLHLAE NYLIRYMPFA 910 920 930 940 950 960 PAIQTFHHLK WTSWDRLKVP AGQPERTLES LLAHLQEQHG LRVRILLHGS ALLYAAGWSP 970 980 990 1000 1010 EKQAQHLPLR VTELVQQLTG QAPAPGQRVL VLELSCEGDD EDTAFPPLHY EL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A gene in the chromosomal region 3p21 with greatly reduced expression in lung cancer is similar to the gene for ubiquitin-activating enzyme." Kok K., Hofstra R., Pilz A., van den Berg A., Terpstra P., Buys C.H.C.M., Carritt B. Proc. Natl. Acad. Sci. U.S.A. 90:6071-6075(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: B-cell.
[2]	"The genomic structure of the human UBE1L gene." Kok K., Van den Berg A., Veldhuis P.M., Franke M., Terpstra P., Buys C.H.C.M. Gene Expr. 4:163-175(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Eye.
[6]	"Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J. Nat. Biotechnol. 21:566-569(2003) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-17. Tissue: Platelet.
[7]	"Influenza B virus NS1 protein inhibits conjugation of the interferon (IFN)-induced ubiquitin-like ISG15 protein." Yuan W., Krug R.M. EMBO J. 20:362-371(2001) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 973-987, INTERACTION WITH G1P2, PATHWAY.
[8]	"Identification of interferon-stimulated gene 15 as an antiviral molecule during Sindbis virus infection in vivo." Lenschow D.J., Giannakopoulos N.V., Gunn L.J., Johnston C., O'Guin A.K., Schmidt R.E., Levine B., Virgin H.W. IV J. Virol. 79:13974-13983(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L13852 mRNA. Translation: AAA75388.1. AF294032 Genomic DNA. Translation: AAG49557.1. BT007026 mRNA. Translation: AAP35672.1. CH471055 Genomic DNA. Translation: EAW65023.1. BC006378 mRNA. Translation: AAH06378.1.
IPI	IPI00013183.
RefSeq	NP_003326.2. NM_003335.2.
UniGene	Hs.16695.
3D structure databases
ProteinModelPortal	P41226.
ModBase	Search...
Protein-protein interaction databases
IntAct	P41226. 1 interaction.
MINT	MINT-1454413.
STRING	9606.ENSP00000333266.
PTM databases
PhosphoSite	P41226.
Polymorphism databases
DMDM	215273977.
Proteomic databases
PaxDb	P41226.
PRIDE	P41226.
Protocols and materials databases
DNASU	7318.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000333486; ENSP00000333266; ENSG00000182179.
GeneID	7318.
KEGG	hsa:7318.
UCSC	uc003cxr.3. human.
Organism-specific databases
CTD	7318.
GeneCards	GC03M049844.
HGNC	HGNC:12471. UBA7.
HPA	CAB015444.
MIM	191325. gene.
neXtProt	NX_P41226.
PharmGKB	PA162407761.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG0476.
HOGENOM	HOG000167329.
HOVERGEN	HBG054199.
InParanoid	P41226.
KO	K10698.
OMA	MPHVTEA.
OrthoDB	EOG45QHCH.
PhylomeDB	P41226.
Enzyme and pathway databases
Reactome	REACT_6900. Immune System.
UniPathway	UPA00143.
Gene expression databases
Bgee	P41226.
CleanEx	HS_UBA7.
Genevestigator	P41226.
GermOnline	ENSG00000182179. Homo sapiens.
Family and domain databases
Gene3D	1.10.3240.10. 1 hit. 3.40.50.720. 4 hits.
InterPro	IPR009036. Molybdenum_cofac_synth_MoeB. IPR016040. NAD(P)-bd_dom. IPR000594. ThiF_NAD_FAD-bd. IPR018965. Ub-activating_enz_e1_C. IPR023280. Ub-like_act_enz_cat_cys_dom. IPR000127. UBact_repeat. IPR019572. Ubiquitin-activating_enzyme. IPR018075. UBQ-activ_enz_E1. IPR018074. UBQ-activ_enz_E1_AS. IPR000011. UBQ/SUMO-activ_enz_E1-like. [Graphical view]
Pfam	PF00899. ThiF. 2 hits. PF09358. UBA_e1_C. 1 hit. PF10585. UBA_e1_thiolCys. 1 hit. PF02134. UBACT. 2 hits. [Graphical view]
PRINTS	PR01849. UBIQUITINACT.
SMART	SM00985. UBA_e1_C. 1 hit. [Graphical view]
SUPFAM	SSF69572. MoeB. 2 hits.
TIGRFAMs	TIGR01408. Ube1. 1 hit.
PROSITE	PS00536. UBIQUITIN_ACTIVAT_1. False negative. PS00865. UBIQUITIN_ACTIVAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	7318.
NextBio	28610.
SOURCE	Search...

Entry information

Entry name

UBA7_HUMAN

Accession

Primary (citable) accession number: P41226
Secondary accession number(s): Q9BRB2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	November 25, 2008
Last modified:	May 1, 2013
This is version 126 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.