Reviewed,
UniProtKB/Swiss-Prot P41226 (UBA7_HUMAN)
Last modified
November 25, 2008.
Version 82.
History...
Clusters with 100%,
90%,
50% identity |
Documents (6) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Ubiquitin-like modifier-activating enzyme 7 Short name=Ubiquitin-activating enzyme 7 Alternative name(s): Ubiquitin-activating enzyme E1 homolog D8 | ||||
| Gene names |
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| Organism | Homo sapiens (Human) | ||||
| Taxonomic identifier | 9606 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 1012 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Activates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP. |
| Pathway | |
| Subunit structure | Monomer By similarity. Binds and is involved in the conjugation of G1P2/ISG15. |
| Tissue specificity | Expressed in a variety of normal and tumor cell types, but is reduced in lung cancer cell lines. |
| Miscellaneous | There are two active sites within the E1 molecule, allowing it to accommodate two ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site. |
| Sequence similarities | Belongs to the ubiquitin-activating E1 family. |
Ontologies
Keywords | |
|---|---|
| Biological process | Ubl conjugation pathway |
| Coding sequence diversity | Polymorphism |
| Domain | Repeat |
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Ligase |
| Technical term | Direct protein sequencing |
Gene Ontology (GO) | |
| Biological process | ISG15-protein conjugation Inferred from direct assay. Source: HGNC ubiquitin-dependent protein catabolic processInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | ATP binding Inferred from electronic annotation. Source: InterPro ISG15 activating enzyme activityInferred from direct assay. Source: HGNC ligase activityInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1012 | 1012 | Ubiquitin-like modifier-activating enzyme 7 | PRO_0000194937 | |||||
Regions | |||||||||
| Repeat | 23 – 159 | 137 | 1-1 | ||||||
| Repeat | 423 – 575 | 153 | 1-2 | ||||||
| Nucleotide binding | 442 – 471 | 30 | ATP By similarity | ||||||
| Region | 23 – 575 | 553 | 2 approximate repeats | ||||||
Sites | |||||||||
| Active site | 599 | 1 | Glycyl thioester intermediate By similarity | ||||||
Natural variations | |||||||||
| Natural variant | 817 | 1 | H → R: dbSNP rs2230149. | VAR_047793 | |||||
Experimental info | |||||||||
| Sequence conflict | 355 – 378 | 24 | GVLSP…KAISR → RCLEPMVACWVSSCPGSAEG NLQ in AAA75388. Ref.1 | ||||||
| Sequence conflict | 355 – 378 | 24 | GVLSP…KAISR → RCLEPMVACWVSSCPGSAEG NLQ in AAG49557. Ref.2 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "A gene in the chromosomal region 3p21 with greatly reduced expression in lung cancer is similar to the gene for ubiquitin-activating enzyme." Kok K., Hofstra R., Pilz A., van den Berg A., Terpstra P., Buys C.H.C.M., Carritt B. Proc. Natl. Acad. Sci. U.S.A. 90:6071-6075(1993) [PubMed: 8327486] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: B-cell. |
| [2] | "The genomic structure of the human UBE1L gene." Kok K., Van den Berg A., Veldhuis P.M., Franke M., Terpstra P., Buys C.H.C.M. Gene Expr. 4:163-175(1995) [PubMed: 7734949] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. |
| [3] | "Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. |
| [4] | Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. Venter J.C.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
| [5] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Eye. |
| [6] | "Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J. Nat. Biotechnol. 21:566-569(2003) [PubMed: 12665801] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-17. Tissue: Platelet. |
| [7] | "Influenza B virus NS1 protein inhibits conjugation of the interferon (IFN)-induced ubiquitin-like ISG15 protein." Yuan W., Krug R.M. EMBO J. 20:362-371(2001) [PubMed: 11157743] [Abstract] Cited for: PROTEIN SEQUENCE OF 973-987, INTERACTION WITH G1P2, PATHWAY. |
| [8] | "Identification of interferon-stimulated gene 15 as an antiviral molecule during Sindbis virus infection in vivo." Lenschow D.J., Giannakopoulos N.V., Gunn L.J., Johnston C., O'Guin A.K., Schmidt R.E., Levine B., Virgin H.W. IV J. Virol. 79:13974-13983(2005) [PubMed: 16254333] [Abstract] Cited for: FUNCTION. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| L13852 mRNA. Translation: AAA75388.1. AF294032 Genomic DNA. Translation: AAG49557.1. BT007026 mRNA. Translation: AAP35672.1. CH471055 Genomic DNA. Translation: EAW65023.1. BC006378 mRNA. Translation: AAH06378.1. | |
| UniGene | Hs.16695 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1JWA based on UniProtKB P12282. |
| ModBase | Search... |
PTM databases | |
| PhosphoSite | P41226. |
Genome annotation databases | |
| Ensembl | ENSG00000182179. Homo sapiens. [Contig view] |
Organism-specific databases | |
| H-InvDB | HIX0022230. |
| HGNC | HGNC:12471. UBA7. |
| HPA | CAB015444. |
| MIM | 191325. gene. |
| PharmGKB | PA37121. |
| GenAtlas | Search... |
| GeneCards | Search... |
Phylogenomic databases | |
| HOVERGEN | P41226. |
Gene expression databases | |
| CleanEx | HS_UBA7. |
| GermOnline | ENSG00000182179. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR016040. NAD(P)-bd. IPR000594. ThiF_NAD_FAD_bd. IPR000127. UBact_repeat. IPR000011. UBQ-activ_enz_E1. [Graphical view] |
| Gene3D | G3DSA:3.40.50.720. NAD(P)-bd. 2 hits. |
| Pfam | PF00899. ThiF. 2 hits. PF02134. UBACT. 2 hits. [Graphical view] |
| PRINTS | PR01849. UBIQUITINACT. |
| TIGRFAMs | TIGR01408. Ube1. 1 hit. |
| PROSITE | PS00536. UBIQUITIN_ACTIVAT_1. False negative. PS00865. UBIQUITIN_ACTIVAT_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| SOURCE | Search... |
Entry information
| Entry name | UBA7_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P41226 Secondary accession number(s): Q9BRB2 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 3 Human chromosome 3: entries, gene names and cross-references to MIM |
| Human entries with polymorphisms or disease mutations List of human entries with polymorphisms or disease mutations |
| Human polymorphisms and disease mutations Index of human polymorphisms and disease mutations |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


