Reviewed, UniProtKB/Swiss-Prot P41226 (UBA7_HUMAN)

Names and origin

Protein names

Recommended name:
    Ubiquitin-like modifier-activating enzyme 7
      Short name=Ubiquitin-activating enzyme 7
Alternative name(s):
    Ubiquitin-activating enzyme E1 homolog
    D8

Gene names

Name:	UBA7
Synonyms:	UBE1L, UBE2

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	1012 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Activates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Monomer By similarity. Binds and is involved in the conjugation of G1P2/ISG15.
Tissue specificity	Expressed in a variety of normal and tumor cell types, but is reduced in lung cancer cell lines.
Miscellaneous	There are two active sites within the E1 molecule, allowing it to accommodate two ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site.
Sequence similarities	Belongs to the ubiquitin-activating E1 family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Coding sequence diversity	Polymorphism
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	ISG15-protein conjugation Inferred from direct assay. Source: HGNC ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro ISG15 activating enzyme activity Inferred from direct assay. Source: HGNC ligase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1012	1012	Ubiquitin-like modifier-activating enzyme 7		PRO_0000194937
Regions
	Repeat	23 – 159	137	1-1
	Repeat	423 – 575	153	1-2
	Nucleotide binding	442 – 471	30	ATP By similarity
	Region	23 – 575	553	2 approximate repeats
Sites
	Active site	599	1	Glycyl thioester intermediate By similarity
Natural variations
	Natural variant	817	1	H → R: dbSNP rs2230149.		VAR_047793
Experimental info
	Sequence conflict	355 – 378	24	GVLSP…KAISR → RCLEPMVACWVSSCPGSAEG NLQ in AAA75388. Ref.1
	Sequence conflict	355 – 378	24	GVLSP…KAISR → RCLEPMVACWVSSCPGSAEG NLQ in AAG49557. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P41226-1 [UniParc]. Last modified November 25, 2008. Version 2. Checksum: A01E1106D81778EB	FASTA	1,012	111,694
	10 20 30 40 50 60 MDALDASKLL DEELYSRQLY VLGSPAMQRI QGARVLVSGL QGLGAEVAKN LVLMGVGSLT 70 80 90 100 110 120 LHDPHPTCWS DLAAQFLLSE QDLERSRAEA SQELLAQLNR AVQVVVHTGD ITEDLLLDFQ 130 140 150 160 170 180 VVVLTAAKLE EQLKVGTLCH KHGVCFLAAD TRGLVGQLFC DFGEDFTVQD PTEAEPLTAA 190 200 210 220 230 240 IQHISQGSPG ILTLRKGANT HYFRDGDLVT FSGIEGMVEL NDCDPRSIHV REDGSLEIGD 250 260 270 280 290 300 TTTFSRYLRG GAITEVKRPK TVRHKSLDTA LLQPHVVAQS SQEVHHAHCL HQAFCALHKF 310 320 330 340 350 360 QHLHGRPPQP WDPVDAETVV GLARDLEPLK RTEEEPLEEP LDEALVRTVA LSSAGVLSPM 370 380 390 400 410 420 VAMLGAVAAQ EVLKAISRKF MPLDQWLYFD ALDCLPEDGE LLPSPEDCAL RGSRYDGQIA 430 440 450 460 470 480 VFGAGFQEKL RRQHYLLVGA GAIGCELLKV FALVGLGAGN SGGLTVVDMD HIERSNLSRQ 490 500 510 520 530 540 FLFRSQDVGR PKAEVAAAAA RGLNPDLQVI PLTYPLDPTT EHIYGDNFFS RVDGVAAALD 550 560 570 580 590 600 SFQARRYVAA RCTHYLKPLL EAGTSGTWGS ATVFMPHVTE AYRAPASAAA SEDAPYPVCT 610 620 630 640 650 660 VRYFPSTAEH TLQWARHEFE ELFRLSAETI NHHQQAHTSL ADMDEPQTLT LLKPVLGVLR 670 680 690 700 710 720 VRPQNWQDCV AWALGHWKLC FHYGIKQLLR HFPPNKVLED GTPFWSGPKQ CPQPLEFDTN 730 740 750 760 770 780 QDTHLLYVLA AANLYAQMHG LPGSQDWTAL RELLKLLPQP DPQQMAPIFA SNLELASASA 790 800 810 820 830 840 EFGPEQQKEL NKALEVWSVG PPLKPLMFEK DDDSNFHVDF VVAAASLRCQ NYGIPPVNRA 850 860 870 880 890 900 QSKRIVGQII PAIATTTAAV AGLLGLELYK VVSGPRPRSA FRHSYLHLAE NYLIRYMPFA 910 920 930 940 950 960 PAIQTFHHLK WTSWDRLKVP AGQPERTLES LLAHLQEQHG LRVRILLHGS ALLYAAGWSP 970 980 990 1000 1010 EKQAQHLPLR VTELVQQLTG QAPAPGQRVL VLELSCEGDD EDTAFPPLHY EL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A gene in the chromosomal region 3p21 with greatly reduced expression in lung cancer is similar to the gene for ubiquitin-activating enzyme." Kok K., Hofstra R., Pilz A., van den Berg A., Terpstra P., Buys C.H.C.M., Carritt B. Proc. Natl. Acad. Sci. U.S.A. 90:6071-6075(1993) [PubMed: 8327486] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: B-cell.
[2]	"The genomic structure of the human UBE1L gene." Kok K., Van den Berg A., Veldhuis P.M., Franke M., Terpstra P., Buys C.H.C.M. Gene Expr. 4:163-175(1995) [PubMed: 7734949] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Eye.
[6]	"Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J. Nat. Biotechnol. 21:566-569(2003) [PubMed: 12665801] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-17. Tissue: Platelet.
[7]	"Influenza B virus NS1 protein inhibits conjugation of the interferon (IFN)-induced ubiquitin-like ISG15 protein." Yuan W., Krug R.M. EMBO J. 20:362-371(2001) [PubMed: 11157743] [Abstract] Cited for: PROTEIN SEQUENCE OF 973-987, INTERACTION WITH G1P2, PATHWAY.
[8]	"Identification of interferon-stimulated gene 15 as an antiviral molecule during Sindbis virus infection in vivo." Lenschow D.J., Giannakopoulos N.V., Gunn L.J., Johnston C., O'Guin A.K., Schmidt R.E., Levine B., Virgin H.W. IV J. Virol. 79:13974-13983(2005) [PubMed: 16254333] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L13852 mRNA. Translation: AAA75388.1. AF294032 Genomic DNA. Translation: AAG49557.1. BT007026 mRNA. Translation: AAP35672.1. CH471055 Genomic DNA. Translation: EAW65023.1. BC006378 mRNA. Translation: AAH06378.1.
UniGene	Hs.16695
3D structure databases
HSSP	HSSP built from PDB template 1JWA based on UniProtKB P12282.
ModBase	Search...
PTM databases
PhosphoSite	P41226.
Genome annotation databases
Ensembl	ENSG00000182179. Homo sapiens. [Contig view]
Organism-specific databases
H-InvDB	HIX0022230.
HGNC	HGNC:12471. UBA7.
HPA	CAB015444.
MIM	191325. gene.
PharmGKB	PA37121.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOVERGEN	P41226.
Gene expression databases
CleanEx	HS_UBA7.
GermOnline	ENSG00000182179. Homo sapiens.
Family and domain databases
InterPro	IPR016040. NAD(P)-bd. IPR000594. ThiF_NAD_FAD_bd. IPR000127. UBact_repeat. IPR000011. UBQ-activ_enz_E1. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 2 hits.
Pfam	PF00899. ThiF. 2 hits. PF02134. UBACT. 2 hits. [Graphical view]
PRINTS	PR01849. UBIQUITINACT.
TIGRFAMs	TIGR01408. Ube1. 1 hit.
PROSITE	PS00536. UBIQUITIN_ACTIVAT_1. False negative. PS00865. UBIQUITIN_ACTIVAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

UBA7_HUMAN

Accession

Primary (citable) accession number: P41226
Secondary accession number(s): Q9BRB2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	November 25, 2008
Last modified:	November 25, 2008
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)