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Protein

Ubiquitin-like modifier-activating enzyme 7

Gene

UBA7

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Activates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Catalyzes the ISGylation of influenza A virus NS1 protein.2 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.1 Publication
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei599Glycyl thioester intermediatePROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi442 – 471ATPBy similarityAdd BLAST30

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciZFISH:HS00051-MONOMER.
ReactomeiR-HSA-1169408. ISG15 antiviral mechanism.
R-HSA-168928. RIG-I/MDA5 mediated induction of IFN-alpha/beta pathways.
R-HSA-5656169. Termination of translesion DNA synthesis.
R-HSA-936440. Negative regulators of RIG-I/MDA5 signaling.
R-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-like modifier-activating enzyme 7
Short name:
Ubiquitin-activating enzyme 7
Alternative name(s):
D8
Ubiquitin-activating enzyme E1 homolog
Gene namesi
Name:UBA7
Synonyms:UBE1L, UBE2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:12471. UBA7.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi7318.
OpenTargetsiENSG00000182179.
PharmGKBiPA162407761.

Chemistry databases

ChEMBLiCHEMBL2321623.

Polymorphism and mutation databases

BioMutaiUBA7.
DMDMi215273977.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001949371 – 1012Ubiquitin-like modifier-activating enzyme 7Add BLAST1012

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei266PhosphoserineCombined sources1

Post-translational modificationi

ISGylated.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP41226.
MaxQBiP41226.
PaxDbiP41226.
PeptideAtlasiP41226.
PRIDEiP41226.

PTM databases

iPTMnetiP41226.
PhosphoSitePlusiP41226.

Expressioni

Tissue specificityi

Expressed in a variety of normal and tumor cell types, but is reduced in lung cancer cell lines.

Gene expression databases

BgeeiENSG00000182179.
CleanExiHS_UBA7.
GenevisibleiP41226. HS.

Organism-specific databases

HPAiCAB015444.

Interactioni

Subunit structurei

Monomer (By similarity). Binds and is involved in the conjugation of G1P2/ISG15.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
ISG15P051618EBI-751921,EBI-746466

Protein-protein interaction databases

BioGridi113166. 10 interactors.
DIPiDIP-60526N.
IntActiP41226. 5 interactors.
MINTiMINT-1454413.
STRINGi9606.ENSP00000333266.

Chemistry databases

BindingDBiP41226.

Structurei

3D structure databases

ProteinModelPortaliP41226.
SMRiP41226.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati23 – 1591-1Add BLAST137
Repeati423 – 5751-2Add BLAST153

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni23 – 5752 approximate repeatsAdd BLAST553

Sequence similaritiesi

Belongs to the ubiquitin-activating E1 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2012. Eukaryota.
COG0476. LUCA.
GeneTreeiENSGT00390000016689.
HOGENOMiHOG000167329.
HOVERGENiHBG054199.
InParanoidiP41226.
KOiK10698.
OMAiFPPLHYE.
OrthoDBiEOG091G0130.
PhylomeDBiP41226.
TreeFamiTF300586.

Family and domain databases

Gene3Di1.10.3240.10. 1 hit.
3.40.50.720. 4 hits.
InterProiIPR032420. E1_4HB.
IPR032418. E1_FCCH.
IPR016040. NAD(P)-bd_dom.
IPR000594. ThiF_NAD_FAD-bd.
IPR018965. Ub-activating_enz_E1_C.
IPR019572. UBA_E1_Cys.
IPR018075. UBQ-activ_enz_E1.
IPR033127. UBQ-activ_enz_E1_Cys_AS.
IPR000011. UBQ/SUMO-activ_enz_E1-like.
[Graphical view]
PfamiPF16191. E1_4HB. 1 hit.
PF16190. E1_FCCH. 1 hit.
PF09358. E1_UFD. 1 hit.
PF00899. ThiF. 2 hits.
PF10585. UBA_e1_thiolCys. 1 hit.
[Graphical view]
PRINTSiPR01849. UBIQUITINACT.
SMARTiSM00985. UBA_e1_C. 1 hit.
[Graphical view]
SUPFAMiSSF69572. SSF69572. 2 hits.
TIGRFAMsiTIGR01408. Ube1. 1 hit.
PROSITEiPS00865. UBIQUITIN_ACTIVAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P41226-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDALDASKLL DEELYSRQLY VLGSPAMQRI QGARVLVSGL QGLGAEVAKN
60 70 80 90 100
LVLMGVGSLT LHDPHPTCWS DLAAQFLLSE QDLERSRAEA SQELLAQLNR
110 120 130 140 150
AVQVVVHTGD ITEDLLLDFQ VVVLTAAKLE EQLKVGTLCH KHGVCFLAAD
160 170 180 190 200
TRGLVGQLFC DFGEDFTVQD PTEAEPLTAA IQHISQGSPG ILTLRKGANT
210 220 230 240 250
HYFRDGDLVT FSGIEGMVEL NDCDPRSIHV REDGSLEIGD TTTFSRYLRG
260 270 280 290 300
GAITEVKRPK TVRHKSLDTA LLQPHVVAQS SQEVHHAHCL HQAFCALHKF
310 320 330 340 350
QHLHGRPPQP WDPVDAETVV GLARDLEPLK RTEEEPLEEP LDEALVRTVA
360 370 380 390 400
LSSAGVLSPM VAMLGAVAAQ EVLKAISRKF MPLDQWLYFD ALDCLPEDGE
410 420 430 440 450
LLPSPEDCAL RGSRYDGQIA VFGAGFQEKL RRQHYLLVGA GAIGCELLKV
460 470 480 490 500
FALVGLGAGN SGGLTVVDMD HIERSNLSRQ FLFRSQDVGR PKAEVAAAAA
510 520 530 540 550
RGLNPDLQVI PLTYPLDPTT EHIYGDNFFS RVDGVAAALD SFQARRYVAA
560 570 580 590 600
RCTHYLKPLL EAGTSGTWGS ATVFMPHVTE AYRAPASAAA SEDAPYPVCT
610 620 630 640 650
VRYFPSTAEH TLQWARHEFE ELFRLSAETI NHHQQAHTSL ADMDEPQTLT
660 670 680 690 700
LLKPVLGVLR VRPQNWQDCV AWALGHWKLC FHYGIKQLLR HFPPNKVLED
710 720 730 740 750
GTPFWSGPKQ CPQPLEFDTN QDTHLLYVLA AANLYAQMHG LPGSQDWTAL
760 770 780 790 800
RELLKLLPQP DPQQMAPIFA SNLELASASA EFGPEQQKEL NKALEVWSVG
810 820 830 840 850
PPLKPLMFEK DDDSNFHVDF VVAAASLRCQ NYGIPPVNRA QSKRIVGQII
860 870 880 890 900
PAIATTTAAV AGLLGLELYK VVSGPRPRSA FRHSYLHLAE NYLIRYMPFA
910 920 930 940 950
PAIQTFHHLK WTSWDRLKVP AGQPERTLES LLAHLQEQHG LRVRILLHGS
960 970 980 990 1000
ALLYAAGWSP EKQAQHLPLR VTELVQQLTG QAPAPGQRVL VLELSCEGDD
1010
EDTAFPPLHY EL
Length:1,012
Mass (Da):111,694
Last modified:November 25, 2008 - v2
Checksum:iA01E1106D81778EB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti355 – 378GVLSP…KAISR → RCLEPMVACWVSSCPGSAEG NLQ in AAA75388 (PubMed:8327486).CuratedAdd BLAST24
Sequence conflicti355 – 378GVLSP…KAISR → RCLEPMVACWVSSCPGSAEG NLQ in AAG49557 (PubMed:7734949).CuratedAdd BLAST24

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_052434712P → S.Corresponds to variant rs11928913dbSNPEnsembl.1
Natural variantiVAR_047793817H → R.Corresponds to variant rs2230149dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13852 mRNA. Translation: AAA75388.1.
AF294032 Genomic DNA. Translation: AAG49557.1.
BT007026 mRNA. Translation: AAP35672.1.
CH471055 Genomic DNA. Translation: EAW65023.1.
BC006378 mRNA. Translation: AAH06378.1.
CCDSiCCDS2805.1.
RefSeqiNP_003326.2. NM_003335.2.
UniGeneiHs.16695.

Genome annotation databases

EnsembliENST00000333486; ENSP00000333266; ENSG00000182179.
GeneIDi7318.
KEGGihsa:7318.
UCSCiuc003cxr.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13852 mRNA. Translation: AAA75388.1.
AF294032 Genomic DNA. Translation: AAG49557.1.
BT007026 mRNA. Translation: AAP35672.1.
CH471055 Genomic DNA. Translation: EAW65023.1.
BC006378 mRNA. Translation: AAH06378.1.
CCDSiCCDS2805.1.
RefSeqiNP_003326.2. NM_003335.2.
UniGeneiHs.16695.

3D structure databases

ProteinModelPortaliP41226.
SMRiP41226.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi113166. 10 interactors.
DIPiDIP-60526N.
IntActiP41226. 5 interactors.
MINTiMINT-1454413.
STRINGi9606.ENSP00000333266.

Chemistry databases

BindingDBiP41226.
ChEMBLiCHEMBL2321623.

PTM databases

iPTMnetiP41226.
PhosphoSitePlusiP41226.

Polymorphism and mutation databases

BioMutaiUBA7.
DMDMi215273977.

Proteomic databases

EPDiP41226.
MaxQBiP41226.
PaxDbiP41226.
PeptideAtlasiP41226.
PRIDEiP41226.

Protocols and materials databases

DNASUi7318.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000333486; ENSP00000333266; ENSG00000182179.
GeneIDi7318.
KEGGihsa:7318.
UCSCiuc003cxr.4. human.

Organism-specific databases

CTDi7318.
DisGeNETi7318.
GeneCardsiUBA7.
HGNCiHGNC:12471. UBA7.
HPAiCAB015444.
MIMi191325. gene.
neXtProtiNX_P41226.
OpenTargetsiENSG00000182179.
PharmGKBiPA162407761.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2012. Eukaryota.
COG0476. LUCA.
GeneTreeiENSGT00390000016689.
HOGENOMiHOG000167329.
HOVERGENiHBG054199.
InParanoidiP41226.
KOiK10698.
OMAiFPPLHYE.
OrthoDBiEOG091G0130.
PhylomeDBiP41226.
TreeFamiTF300586.

Enzyme and pathway databases

UniPathwayiUPA00143.
BioCyciZFISH:HS00051-MONOMER.
ReactomeiR-HSA-1169408. ISG15 antiviral mechanism.
R-HSA-168928. RIG-I/MDA5 mediated induction of IFN-alpha/beta pathways.
R-HSA-5656169. Termination of translesion DNA synthesis.
R-HSA-936440. Negative regulators of RIG-I/MDA5 signaling.
R-HSA-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

ChiTaRSiUBA7. human.
GeneWikiiUBE1L.
GenomeRNAii7318.
PROiP41226.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000182179.
CleanExiHS_UBA7.
GenevisibleiP41226. HS.

Family and domain databases

Gene3Di1.10.3240.10. 1 hit.
3.40.50.720. 4 hits.
InterProiIPR032420. E1_4HB.
IPR032418. E1_FCCH.
IPR016040. NAD(P)-bd_dom.
IPR000594. ThiF_NAD_FAD-bd.
IPR018965. Ub-activating_enz_E1_C.
IPR019572. UBA_E1_Cys.
IPR018075. UBQ-activ_enz_E1.
IPR033127. UBQ-activ_enz_E1_Cys_AS.
IPR000011. UBQ/SUMO-activ_enz_E1-like.
[Graphical view]
PfamiPF16191. E1_4HB. 1 hit.
PF16190. E1_FCCH. 1 hit.
PF09358. E1_UFD. 1 hit.
PF00899. ThiF. 2 hits.
PF10585. UBA_e1_thiolCys. 1 hit.
[Graphical view]
PRINTSiPR01849. UBIQUITINACT.
SMARTiSM00985. UBA_e1_C. 1 hit.
[Graphical view]
SUPFAMiSSF69572. SSF69572. 2 hits.
TIGRFAMsiTIGR01408. Ube1. 1 hit.
PROSITEiPS00865. UBIQUITIN_ACTIVAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBA7_HUMAN
AccessioniPrimary (citable) accession number: P41226
Secondary accession number(s): Q9BRB2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 25, 2008
Last modified: November 30, 2016
This is version 162 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

There are two active sites within the E1 molecule, allowing it to accommodate two ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.