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Protein

Ubiquitin-like modifier-activating enzyme 7

Gene

UBA7

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Activates ubiquitin by first adenylating with ATP its C-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Catalyzes the ISGylation of influenza A virus NS1 protein.2 Publications

Miscellaneous

There are two active sites within the E1 molecule, allowing it to accommodate two ubiquitin moieties at a time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.1 Publication
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei599Glycyl thioester intermediatePROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi442 – 471ATPBy similarityAdd BLAST30

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processUbl conjugation pathway
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-1169408 ISG15 antiviral mechanism
R-HSA-168928 DDX58/IFIH1-mediated induction of interferon-alpha/beta
R-HSA-5656169 Termination of translesion DNA synthesis
R-HSA-936440 Negative regulators of DDX58/IFIH1 signaling
R-HSA-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-like modifier-activating enzyme 7
Short name:
Ubiquitin-activating enzyme 7
Alternative name(s):
D8
Ubiquitin-activating enzyme E1 homolog
Gene namesi
Name:UBA7
Synonyms:UBE1L, UBE2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

EuPathDBiHostDB:ENSG00000182179.10
HGNCiHGNC:12471 UBA7
MIMi191325 gene
neXtProtiNX_P41226

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Organism-specific databases

DisGeNETi7318
OpenTargetsiENSG00000182179
PharmGKBiPA162407761

Chemistry databases

ChEMBLiCHEMBL2321623

Polymorphism and mutation databases

BioMutaiUBA7
DMDMi215273977

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001949371 – 1012Ubiquitin-like modifier-activating enzyme 7Add BLAST1012

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei266PhosphoserineCombined sources1

Post-translational modificationi

ISGylated.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP41226
MaxQBiP41226
PaxDbiP41226
PeptideAtlasiP41226
PRIDEiP41226

PTM databases

iPTMnetiP41226
PhosphoSitePlusiP41226

Expressioni

Tissue specificityi

Expressed in a variety of normal and tumor cell types, but is reduced in lung cancer cell lines.

Gene expression databases

BgeeiENSG00000182179
CleanExiHS_UBA7
GenevisibleiP41226 HS

Organism-specific databases

HPAiCAB015444
HPA058182

Interactioni

Subunit structurei

Monomer (By similarity). Binds and is involved in the conjugation of G1P2/ISG15.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
ISG15P0516111EBI-751921,EBI-746466

Protein-protein interaction databases

BioGridi113166, 10 interactors
DIPiDIP-60526N
IntActiP41226, 5 interactors
MINTiP41226
STRINGi9606.ENSP00000333266

Chemistry databases

BindingDBiP41226

Structurei

3D structure databases

ProteinModelPortaliP41226
SMRiP41226
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati23 – 1591-1Add BLAST137
Repeati423 – 5751-2Add BLAST153

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni23 – 5752 approximate repeatsAdd BLAST553

Sequence similaritiesi

Belongs to the ubiquitin-activating E1 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG2012 Eukaryota
COG0476 LUCA
GeneTreeiENSGT00390000016689
HOGENOMiHOG000167329
HOVERGENiHBG054199
InParanoidiP41226
KOiK10698
OMAiAFRHSYL
OrthoDBiEOG091G0130
PhylomeDBiP41226
TreeFamiTF300586

Family and domain databases

Gene3Di3.10.290.60, 1 hit
InterProiView protein in InterPro
IPR032420 E1_4HB
IPR032418 E1_FCCH
IPR000594 ThiF_NAD_FAD-bd
IPR018965 Ub-activating_enz_E1_C
IPR038252 UBA_E1_C_sf
IPR019572 UBA_E1_Cys
IPR035985 Ubiquitin-activating_enz
IPR018075 UBQ-activ_enz_E1
IPR033127 UBQ-activ_enz_E1_Cys_AS
IPR000011 UBQ/SUMO-activ_enz_E1-like
PfamiView protein in Pfam
PF16191 E1_4HB, 1 hit
PF16190 E1_FCCH, 1 hit
PF09358 E1_UFD, 1 hit
PF00899 ThiF, 2 hits
PF10585 UBA_e1_thiolCys, 1 hit
PRINTSiPR01849 UBIQUITINACT
SMARTiView protein in SMART
SM00985 UBA_e1_C, 1 hit
SUPFAMiSSF69572 SSF69572, 2 hits
TIGRFAMsiTIGR01408 Ube1, 1 hit
PROSITEiView protein in PROSITE
PS00865 UBIQUITIN_ACTIVAT_2, 1 hit

Sequencei

Sequence statusi: Complete.

P41226-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDALDASKLL DEELYSRQLY VLGSPAMQRI QGARVLVSGL QGLGAEVAKN
60 70 80 90 100
LVLMGVGSLT LHDPHPTCWS DLAAQFLLSE QDLERSRAEA SQELLAQLNR
110 120 130 140 150
AVQVVVHTGD ITEDLLLDFQ VVVLTAAKLE EQLKVGTLCH KHGVCFLAAD
160 170 180 190 200
TRGLVGQLFC DFGEDFTVQD PTEAEPLTAA IQHISQGSPG ILTLRKGANT
210 220 230 240 250
HYFRDGDLVT FSGIEGMVEL NDCDPRSIHV REDGSLEIGD TTTFSRYLRG
260 270 280 290 300
GAITEVKRPK TVRHKSLDTA LLQPHVVAQS SQEVHHAHCL HQAFCALHKF
310 320 330 340 350
QHLHGRPPQP WDPVDAETVV GLARDLEPLK RTEEEPLEEP LDEALVRTVA
360 370 380 390 400
LSSAGVLSPM VAMLGAVAAQ EVLKAISRKF MPLDQWLYFD ALDCLPEDGE
410 420 430 440 450
LLPSPEDCAL RGSRYDGQIA VFGAGFQEKL RRQHYLLVGA GAIGCELLKV
460 470 480 490 500
FALVGLGAGN SGGLTVVDMD HIERSNLSRQ FLFRSQDVGR PKAEVAAAAA
510 520 530 540 550
RGLNPDLQVI PLTYPLDPTT EHIYGDNFFS RVDGVAAALD SFQARRYVAA
560 570 580 590 600
RCTHYLKPLL EAGTSGTWGS ATVFMPHVTE AYRAPASAAA SEDAPYPVCT
610 620 630 640 650
VRYFPSTAEH TLQWARHEFE ELFRLSAETI NHHQQAHTSL ADMDEPQTLT
660 670 680 690 700
LLKPVLGVLR VRPQNWQDCV AWALGHWKLC FHYGIKQLLR HFPPNKVLED
710 720 730 740 750
GTPFWSGPKQ CPQPLEFDTN QDTHLLYVLA AANLYAQMHG LPGSQDWTAL
760 770 780 790 800
RELLKLLPQP DPQQMAPIFA SNLELASASA EFGPEQQKEL NKALEVWSVG
810 820 830 840 850
PPLKPLMFEK DDDSNFHVDF VVAAASLRCQ NYGIPPVNRA QSKRIVGQII
860 870 880 890 900
PAIATTTAAV AGLLGLELYK VVSGPRPRSA FRHSYLHLAE NYLIRYMPFA
910 920 930 940 950
PAIQTFHHLK WTSWDRLKVP AGQPERTLES LLAHLQEQHG LRVRILLHGS
960 970 980 990 1000
ALLYAAGWSP EKQAQHLPLR VTELVQQLTG QAPAPGQRVL VLELSCEGDD
1010
EDTAFPPLHY EL
Length:1,012
Mass (Da):111,694
Last modified:November 25, 2008 - v2
Checksum:iA01E1106D81778EB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti355 – 378GVLSP…KAISR → RCLEPMVACWVSSCPGSAEG NLQ in AAA75388 (PubMed:8327486).CuratedAdd BLAST24
Sequence conflicti355 – 378GVLSP…KAISR → RCLEPMVACWVSSCPGSAEG NLQ in AAG49557 (PubMed:7734949).CuratedAdd BLAST24

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_052434712P → S. Corresponds to variant dbSNP:rs11928913Ensembl.1
Natural variantiVAR_047793817H → R. Corresponds to variant dbSNP:rs2230149Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13852 mRNA Translation: AAA75388.1
AF294032 Genomic DNA Translation: AAG49557.1
BT007026 mRNA Translation: AAP35672.1
CH471055 Genomic DNA Translation: EAW65023.1
BC006378 mRNA Translation: AAH06378.1
CCDSiCCDS2805.1
RefSeqiNP_003326.2, NM_003335.2
UniGeneiHs.16695

Genome annotation databases

EnsembliENST00000333486; ENSP00000333266; ENSG00000182179
GeneIDi7318
KEGGihsa:7318
UCSCiuc003cxr.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiUBA7_HUMAN
AccessioniPrimary (citable) accession number: P41226
Secondary accession number(s): Q9BRB2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 25, 2008
Last modified: May 23, 2018
This is version 173 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health