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Protein

Thyrotroph embryonic factor

Gene

Tef

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcription factor that binds to and transactivates the TSHB promoter. Binds to a minimal DNA-binding sequence 5'-[TC][AG][AG]TTA[TC][AG]-3'.

GO - Molecular functioni

  • double-stranded DNA binding Source: RGD
  • protein heterodimerization activity Source: RGD
  • protein homodimerization activity Source: RGD
  • RNA polymerase II proximal promoter sequence-specific DNA binding Source: NTNU_SB
  • sequence-specific DNA binding Source: RGD
  • transcriptional activator activity, RNA polymerase II proximal promoter sequence-specific DNA binding Source: NTNU_SB

GO - Biological processi

Keywordsi

Molecular functionActivator, DNA-binding
Biological processBiological rhythms, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Thyrotroph embryonic factor
Gene namesi
Name:Tef
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi3841 Tef

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi218K → A: 30-fold decrease in affinity for prolactin recognition element. 1 Publication1
Mutagenesisi219K → A: 30-fold decrease in affinity for prolactin recognition element. 1 Publication1
Mutagenesisi221K → A: 30-fold decrease in affinity for prolactin recognition element. 1 Publication1
Mutagenesisi222K → A: 30-fold decrease in affinity for prolactin recognition element. 1 Publication1
Mutagenesisi266L → V: Diminishes DNA-binding. 1 Publication1
Mutagenesisi273L → V: Diminishes DNA-binding. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000765151 – 301Thyrotroph embryonic factorAdd BLAST301

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei30PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP41224

Expressioni

Tissue specificityi

Expressed exclusively in the rostral portion of the anterior pituitary during embryogenesis. Found in several tissues in juvenile and adult rats.

Developmental stagei

Expressed up to embryonic day 14 and specifically in the anterior pituitary during embryogenesis.

Inductioni

Accumulates according to a robust circadian rhythm.By similarity

Gene expression databases

BgeeiENSRNOG00000019383
ExpressionAtlasiP41224 baseline and differential
GenevisibleiP41224 RN

Interactioni

Subunit structurei

Binds DNA as a homodimer or a heterodimer. Can form a heterodimer with DBP.

GO - Molecular functioni

  • protein heterodimerization activity Source: RGD
  • protein homodimerization activity Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026258

Structurei

3D structure databases

ProteinModelPortaliP41224
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini231 – 294bZIPPROSITE-ProRule annotationAdd BLAST64

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni233 – 253Basic motifPROSITE-ProRule annotationAdd BLAST21
Regioni254 – 261Leucine-zipperPROSITE-ProRule annotation8

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi164 – 215Pro-rich (proline/acidic region (PAR))Add BLAST52

Sequence similaritiesi

Belongs to the bZIP family. PAR subfamily.Curated

Phylogenomic databases

eggNOGiKOG3119 Eukaryota
ENOG410YJ8G LUCA
GeneTreeiENSGT00440000039070
HOGENOMiHOG000234383
HOVERGENiHBG004983
InParanoidiP41224
KOiK09058
PhylomeDBiP41224
TreeFamiTF315869

Family and domain databases

InterProiView protein in InterPro
IPR004827 bZIP
IPR029832 TEF/VBP
PANTHERiPTHR11988:SF24 PTHR11988:SF24, 1 hit
PfamiView protein in Pfam
PF07716 bZIP_2, 1 hit
SMARTiView protein in SMART
SM00338 BRLZ, 1 hit
PROSITEiView protein in PROSITE
PS50217 BZIP, 1 hit

Sequencei

Sequence statusi: Complete.

P41224-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEAGGGKKP PVEPQAGPGP GRAAGERGLS GSFPLVLKKL MENPPRETRL
60 70 80 90 100
DKEKGKEKLE EDESAAASTM AVSASLMPPI WDKTIPYDGE SFHLEYMDLD
110 120 130 140 150
EFLLENGIPA SPTHLAQNLL LPVAELEGKE SASSSTASPP SSSTAIFQPS
160 170 180 190 200
ETVSSTESSL EKERETPSPI DPNCVEVDVN FNPDPADLVL SSVPGGELFN
210 220 230 240 250
PRKHKFAEED LKPQPMIKKA KKVFVPDEQK DEKYWTRRKK NNVAAKRSRD
260 270 280 290 300
ARRLKENQIT IRAAFLEKEN TALRTEVAEL RKEVGKCKTI VSKYETKYGP

L
Length:301
Mass (Da):33,159
Last modified:December 6, 2005 - v3
Checksum:i2EDFB924F6D2D5F3
GO

Sequence cautioni

The sequence AAB20032 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC101895 mRNA Translation: AAI01896.1
S58745 mRNA Translation: AAB20032.1 Different initiation.
PIRiA40579
RefSeqiNP_062067.2, NM_019194.2
UniGeneiRn.59871

Genome annotation databases

EnsembliENSRNOT00000026258; ENSRNOP00000026258; ENSRNOG00000019383
GeneIDi29362
KEGGirno:29362
UCSCiRGD:3841 rat

Similar proteinsi

Entry informationi

Entry nameiTEF_RAT
AccessioniPrimary (citable) accession number: P41224
Secondary accession number(s): Q3T1I8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: December 6, 2005
Last modified: March 28, 2018
This is version 129 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health