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Protein

Thyrotroph embryonic factor

Gene

Tef

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor that binds to and transactivates the TSHB promoter. Binds to a minimal DNA-binding sequence 5'-[TC][AG][AG]TTA[TC][AG]-3'.

GO - Molecular functioni

  • protein heterodimerization activity Source: RGD
  • protein homodimerization activity Source: RGD
  • RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: NTNU_SB
  • sequence-specific DNA binding Source: RGD
  • transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding Source: NTNU_SB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Biological rhythms, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Thyrotroph embryonic factor
Gene namesi
Name:Tef
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi3841. Tef.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi218 – 2181K → A: 30-fold decrease in affinity for prolactin recognition element. 1 Publication
Mutagenesisi219 – 2191K → A: 30-fold decrease in affinity for prolactin recognition element. 1 Publication
Mutagenesisi221 – 2211K → A: 30-fold decrease in affinity for prolactin recognition element. 1 Publication
Mutagenesisi222 – 2221K → A: 30-fold decrease in affinity for prolactin recognition element. 1 Publication
Mutagenesisi266 – 2661L → V: Diminishes DNA-binding. 1 Publication
Mutagenesisi273 – 2731L → V: Diminishes DNA-binding. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 301301Thyrotroph embryonic factorPRO_0000076515Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei30 – 301PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP41224.

Expressioni

Tissue specificityi

Expressed exclusively in the rostral portion of the anterior pituitary during embryogenesis. Found in several tissues in juvenile and adult rats.

Developmental stagei

Expressed up to embryonic day 14 and specifically in the anterior pituitary during embryogenesis.

Inductioni

Accumulates according to a robust circadian rhythm.By similarity

Gene expression databases

ExpressionAtlasiP41224. baseline and differential.
GenevisibleiP41224. RN.

Interactioni

Subunit structurei

Binds DNA as a homodimer or a heterodimer. Can form a heterodimer with DBP.

GO - Molecular functioni

  • protein heterodimerization activity Source: RGD
  • protein homodimerization activity Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026258.

Structurei

3D structure databases

ProteinModelPortaliP41224.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini231 – 29464bZIPPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni233 – 25321Basic motifPROSITE-ProRule annotationAdd
BLAST
Regioni254 – 2618Leucine-zipperPROSITE-ProRule annotation

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi164 – 21552Pro-rich (proline/acidic region (PAR))Add
BLAST

Sequence similaritiesi

Belongs to the bZIP family. PAR subfamily.Curated
Contains 1 bZIP (basic-leucine zipper) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3119. Eukaryota.
ENOG410YJ8G. LUCA.
GeneTreeiENSGT00440000039070.
HOGENOMiHOG000234383.
HOVERGENiHBG004983.
InParanoidiP41224.
KOiK09058.
OMAiCKNIVSK.
OrthoDBiEOG712TZJ.
PhylomeDBiP41224.
TreeFamiTF315869.

Family and domain databases

InterProiIPR004827. bZIP.
IPR029832. TEF/VBP.
[Graphical view]
PANTHERiPTHR11988:SF24. PTHR11988:SF24. 1 hit.
PfamiPF07716. bZIP_2. 1 hit.
[Graphical view]
SMARTiSM00338. BRLZ. 1 hit.
[Graphical view]
PROSITEiPS50217. BZIP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P41224-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEAGGGKKP PVEPQAGPGP GRAAGERGLS GSFPLVLKKL MENPPRETRL
60 70 80 90 100
DKEKGKEKLE EDESAAASTM AVSASLMPPI WDKTIPYDGE SFHLEYMDLD
110 120 130 140 150
EFLLENGIPA SPTHLAQNLL LPVAELEGKE SASSSTASPP SSSTAIFQPS
160 170 180 190 200
ETVSSTESSL EKERETPSPI DPNCVEVDVN FNPDPADLVL SSVPGGELFN
210 220 230 240 250
PRKHKFAEED LKPQPMIKKA KKVFVPDEQK DEKYWTRRKK NNVAAKRSRD
260 270 280 290 300
ARRLKENQIT IRAAFLEKEN TALRTEVAEL RKEVGKCKTI VSKYETKYGP

L
Length:301
Mass (Da):33,159
Last modified:December 6, 2005 - v3
Checksum:i2EDFB924F6D2D5F3
GO

Sequence cautioni

The sequence AAB20032.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC101895 mRNA. Translation: AAI01896.1.
S58745 mRNA. Translation: AAB20032.1. Different initiation.
PIRiA40579.
RefSeqiNP_062067.2. NM_019194.2.
UniGeneiRn.59871.

Genome annotation databases

EnsembliENSRNOT00000026258; ENSRNOP00000026258; ENSRNOG00000019383.
GeneIDi29362.
KEGGirno:29362.
UCSCiRGD:3841. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC101895 mRNA. Translation: AAI01896.1.
S58745 mRNA. Translation: AAB20032.1. Different initiation.
PIRiA40579.
RefSeqiNP_062067.2. NM_019194.2.
UniGeneiRn.59871.

3D structure databases

ProteinModelPortaliP41224.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000026258.

Proteomic databases

PaxDbiP41224.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000026258; ENSRNOP00000026258; ENSRNOG00000019383.
GeneIDi29362.
KEGGirno:29362.
UCSCiRGD:3841. rat.

Organism-specific databases

CTDi7008.
RGDi3841. Tef.

Phylogenomic databases

eggNOGiKOG3119. Eukaryota.
ENOG410YJ8G. LUCA.
GeneTreeiENSGT00440000039070.
HOGENOMiHOG000234383.
HOVERGENiHBG004983.
InParanoidiP41224.
KOiK09058.
OMAiCKNIVSK.
OrthoDBiEOG712TZJ.
PhylomeDBiP41224.
TreeFamiTF315869.

Miscellaneous databases

PROiP41224.

Gene expression databases

ExpressionAtlasiP41224. baseline and differential.
GenevisibleiP41224. RN.

Family and domain databases

InterProiIPR004827. bZIP.
IPR029832. TEF/VBP.
[Graphical view]
PANTHERiPTHR11988:SF24. PTHR11988:SF24. 1 hit.
PfamiPF07716. bZIP_2. 1 hit.
[Graphical view]
SMARTiSM00338. BRLZ. 1 hit.
[Graphical view]
PROSITEiPS50217. BZIP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Prostate.
  2. "TEF, a transcription factor expressed specifically in the anterior pituitary during embryogenesis, defines a new class of leucine zipper proteins."
    Drolet D.W., Scully K.M., Simmons D.M., Wegner M., Chu K., Swanson L.W., Rosenfeld M.G.
    Genes Dev. 5:1739-1753(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 31-301, INTERACTION WITH DBP, MUTAGENESIS.
    Tissue: Pituitary.

Entry informationi

Entry nameiTEF_RAT
AccessioniPrimary (citable) accession number: P41224
Secondary accession number(s): Q3T1I8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: December 6, 2005
Last modified: July 6, 2016
This is version 120 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.