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Protein

Regulator of G-protein signaling 2

Gene

RGS2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulates G protein-coupled receptor signaling cascades. Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form (PubMed:11063746, PubMed:19478087). Plays a role in the regulation of blood pressure in response to signaling via G protein-coupled receptors and GNAQ. Plays a role in regulating the constriction and relaxation of vascular smooth muscle (By similarity). Binds EIF2B5 and blocks its activity, thereby inhibiting the translation of mRNA into protein (PubMed:19736320).By similarity1 Publication4 Publications

GO - Molecular functioni

  • calmodulin binding Source: ProtInc
  • G-protein alpha-subunit binding Source: UniProtKB
  • GTPase activator activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation, Signal transduction inhibitor

Keywords - Biological processi

Cell cycle, Translation regulation

Enzyme and pathway databases

BioCyciZFISH:ENSG00000116741-MONOMER.
ReactomeiR-HSA-416476. G alpha (q) signalling events.
SIGNORiP41220.

Names & Taxonomyi

Protein namesi
Recommended name:
Regulator of G-protein signaling 2
Short name:
RGS2
Alternative name(s):
Cell growth-inhibiting gene 31 protein
G0/G1 switch regulatory protein 8
Gene namesi
Name:RGS2
Synonyms:G0S8
ORF Names:GIG31
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:9998. RGS2.

Subcellular locationi

Isoform 1 :
Isoform 2 :
  • Cell membrane 1 Publication
  • Cytoplasm 1 Publication
  • Nucleusnucleolus 1 Publication
Isoform 3 :
  • Cell membrane 1 Publication
  • Cytoplasm 1 Publication
  • Nucleusnucleolus 1 Publication
Isoform 4 :
  • Cell membrane 1 Publication
  • Mitochondrion 1 Publication

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytoplasmic side of plasma membrane Source: BHF-UCL
  • cytosol Source: BHF-UCL
  • mitochondrion Source: UniProtKB-SubCell
  • neuron projection Source: Ensembl
  • nucleolus Source: UniProtKB-SubCell
  • nucleus Source: UniProtKB
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Mitochondrion, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1M → L: Loss of isoform 1 expression. 1 Publication1
Mutagenesisi5M → L: Loss of isoform 2 expression. 1 Publication1
Mutagenesisi16M → L: Loss of isoform 3 expression. 1 Publication1
Mutagenesisi33M → L: Loss of isoform 4 expression. 1 Publication1
Mutagenesisi37L → D: Impairs association with plasma membrane. 1 Publication1
Mutagenesisi38L → D: Impairs association with plasma membrane. 1 Publication1
Mutagenesisi41W → D: Impairs association with plasma membrane. 1 Publication1
Mutagenesisi45L → D: Impairs association with plasma membrane. 1 Publication1
Mutagenesisi48F → D: Impairs association with plasma membrane. 1 Publication1
Mutagenesisi49L → D: Impairs association with plasma membrane. 1 Publication1
Mutagenesisi79L → A: Near loss of EIF2B5 binding and inhibition of in vitro translation; when associated with E-86; L-87; S-90; K-102; F-105; I-110; E-111 and L-114. 1 Publication1
Mutagenesisi86E → A: Near loss of EIF2B5 binding and inhibition of in vitro translation; when associated with L-79; L-87; S-90; K-102; F-105; I-110; E-111 and L-114. 1 Publication1
Mutagenesisi87L → A: Near loss of EIF2B5 binding and inhibition of in vitro translation; when associated with L-79; E-86; S-90; K-102; F-105; I-110; E-111 and L-114. 1 Publication1
Mutagenesisi90S → A: Near loss of EIF2B5 binding and inhibition of in vitro translation; when associated with L-79; E-86; L-87; K-102; F-105; I-110; E-111 and L-114. 1 Publication1
Mutagenesisi102K → A: Near loss of EIF2B5 binding and inhibition of in vitro translation; when associated with L-79; E-86; L-87; S-90; F-105; I-110; E-111 and L-114. 1 Publication1
Mutagenesisi105F → A: Near loss of EIF2B5 binding and inhibition of in vitro translation; when associated with L-79; E-86; L-87; S-90; K-102; I-110; E-111 and L-114. 1 Publication1
Mutagenesisi106C → S: Changes specificity and confers GNAI1 binding; when associated with D-184. Strongly increases affinity for GNAI1 and GNAI3; when associated with D-184 and K-191. 2 Publications1
Mutagenesisi110I → A: Near loss of EIF2B5 binding and inhibition of in vitro translation; when associated with L-79; E-86; L-87; S-90; K-102; F-105; E-111 and L-114. 1 Publication1
Mutagenesisi111E → A: Near loss of EIF2B5 binding and inhibition of in vitro translation; when associated with L-79; E-86; L-87; S-90; K-102; F-105; I-110 and L-114. 1 Publication1
Mutagenesisi114L → A: Near loss of EIF2B5 binding and inhibition of in vitro translation; when associated with L-79; E-86; L-87; S-90; K-102; F-105; I-110 and E-111. 1 Publication1
Mutagenesisi149N → A: Decreases GTPase accelerating function but has no effect on translation inhibitory activity, suggesting that its role in translation is independent of its effects on G proteins. 1 Publication1
Mutagenesisi184N → D: Changes specificity and confers GNAI1 binding; when associated with D-184. Strongly increases affinity for GNAI1 and GNAI3; when associated with S-106 and K-191. 2 Publications1
Mutagenesisi191E → K: Strongly increases affinity for GNAI1 and GNAI3; when associated with S-106 and D-184. 1 Publication1

Organism-specific databases

DisGeNETi5997.
MalaCardsiRGS2.
OpenTargetsiENSG00000116741.
PharmGKBiPA34372.

Polymorphism and mutation databases

BioMutaiRGS2.
DMDMi729545.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002041781 – 211Regulator of G-protein signaling 2Add BLAST211

Post-translational modificationi

Phosphorylated by protein kinase C. Phosphorylation by PRKG1 leads to activation of RGS2 activity.2 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP41220.
PaxDbiP41220.
PeptideAtlasiP41220.
PRIDEiP41220.

PTM databases

iPTMnetiP41220.
PhosphoSitePlusiP41220.
SwissPalmiP41220.

Expressioni

Tissue specificityi

Expressed in acute myelogenous leukemia (AML) and in acute lymphoblastic leukemia (ALL).1 Publication

Gene expression databases

BgeeiENSG00000116741.
CleanExiHS_RGS2.
ExpressionAtlasiP41220. baseline and differential.
GenevisibleiP41220. HS.

Organism-specific databases

HPAiHPA013385.

Interactioni

Subunit structurei

Interacts with GNAQ (PubMed:18434541, PubMed:19478087). Does not interact with GNAI1 and GNAI3 (PubMed:18434541, PubMed:19478087). Interacts with EIF2B5 (PubMed:19736320). Interacts with PRKG1 (isoform alpha) (PubMed:14608379).4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
LRRK2Q5S0076EBI-712388,EBI-5323863
MTUS2Q5JR593EBI-712388,EBI-742948
PPP1R9AQ9ULJ83EBI-712388,EBI-2515561

GO - Molecular functioni

  • calmodulin binding Source: ProtInc
  • G-protein alpha-subunit binding Source: UniProtKB

Protein-protein interaction databases

BioGridi111929. 67 interactors.
DIPiDIP-44289N.
IntActiP41220. 47 interactors.
MINTiMINT-1380095.
STRINGi9606.ENSP00000235382.

Structurei

Secondary structure

1211
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi74 – 79Combined sources6
Turni80 – 82Combined sources3
Helixi84 – 89Combined sources6
Helixi91 – 103Combined sources13
Helixi108 – 120Combined sources13
Helixi125 – 139Combined sources15
Helixi152 – 161Combined sources10
Helixi162 – 164Combined sources3
Turni167 – 170Combined sources4
Helixi171 – 183Combined sources13
Helixi185 – 191Combined sources7
Helixi193 – 199Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AF0X-ray2.30A71-203[»]
2V4ZX-ray2.80B71-209[»]
4EKCX-ray7.40B/D72-203[»]
4EKDX-ray2.71B72-203[»]
ProteinModelPortaliP41220.
SMRiP41220.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP41220.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini83 – 199RGSPROSITE-ProRule annotationAdd BLAST117

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni32 – 66Necessary for membrane association1 PublicationAdd BLAST35
Regioni79 – 116Necessary to inhibit protein synthesis1 PublicationAdd BLAST38

Sequence similaritiesi

Contains 1 RGS domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3589. Eukaryota.
ENOG410YMJD. LUCA.
GeneTreeiENSGT00760000118903.
HOGENOMiHOG000233512.
HOVERGENiHBG013233.
InParanoidiP41220.
KOiK18154.
OMAiAVQHDCV.
OrthoDBiEOG091G0BDG.
PhylomeDBiP41220.
TreeFamiTF315837.

Family and domain databases

Gene3Di1.10.196.10. 2 hits.
InterProiIPR016137. RGS.
IPR024066. RGS_subdom1.
[Graphical view]
PfamiPF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR01301. RGSPROTEIN.
SMARTiSM00315. RGS. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
PROSITEiPS50132. RGS. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform 1 (identifier: P41220-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQSAMFLAVQ HDCRPMDKSA GSGHKSEEKR EKMKRTLLKD WKTRLSYFLQ
60 70 80 90 100
NSSTPGKPKT GKKSKQQAFI KPSPEEAQLW SEAFDELLAS KYGLAAFRAF
110 120 130 140 150
LKSEFCEENI EFWLACEDFK KTKSPQKLSS KARKIYTDFI EKEAPKEINI
160 170 180 190 200
DFQTKTLIAQ NIQEATSGCF TTAQKRVYSL MENNSYPRFL ESEFYQDLCK
210
KPQITTEPHA T
Length:211
Mass (Da):24,382
Last modified:February 1, 1995 - v1
Checksum:iEFFE4AE47EF9AD8F
GO
Isoform 2 (identifier: P41220-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-4: Missing.

Show »
Length:207
Mass (Da):23,964
Checksum:iE5DCCB2F18E09F51
GO
Isoform 3 (identifier: P41220-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-15: Missing.

Note: Lacks type V adenylyl cyclase (AC) inhibitory function.
Show »
Length:196
Mass (Da):22,666
Checksum:i2F5BF7609CD4172A
GO
Isoform 4 (identifier: P41220-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-32: Missing.

Note: Lacks type V adenylyl cyclase (AC) inhibitory function.
Show »
Length:179
Mass (Da):20,780
Checksum:i6CF0306689DC7A54
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0412981 – 32Missing in isoform 4. 1 PublicationAdd BLAST32
Alternative sequenceiVSP_0412971 – 15Missing in isoform 3. 1 PublicationAdd BLAST15
Alternative sequenceiVSP_0412961 – 4Missing in isoform 2. 1 Publication4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13391 Genomic DNA. Translation: AAA20680.1.
L13463 mRNA. Translation: AAC37587.1.
AF493926 mRNA. Translation: AAM12640.1.
AY971351 mRNA. Translation: AAY40361.1.
AK313668 mRNA. Translation: BAG36420.1.
BT007065 mRNA. Translation: AAP35728.1.
CR457410 mRNA. Translation: CAG33691.1.
AL035407 Genomic DNA. Translation: CAB62512.1.
CH471067 Genomic DNA. Translation: EAW91231.1.
BC007049 mRNA. Translation: AAH07049.1.
CCDSiCCDS1377.1. [P41220-1]
PIRiI53020.
RefSeqiNP_002914.1. NM_002923.3. [P41220-1]
UniGeneiHs.78944.

Genome annotation databases

EnsembliENST00000235382; ENSP00000235382; ENSG00000116741. [P41220-1]
GeneIDi5997.
KEGGihsa:5997.
UCSCiuc001gsl.4. human. [P41220-1]

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L13391 Genomic DNA. Translation: AAA20680.1.
L13463 mRNA. Translation: AAC37587.1.
AF493926 mRNA. Translation: AAM12640.1.
AY971351 mRNA. Translation: AAY40361.1.
AK313668 mRNA. Translation: BAG36420.1.
BT007065 mRNA. Translation: AAP35728.1.
CR457410 mRNA. Translation: CAG33691.1.
AL035407 Genomic DNA. Translation: CAB62512.1.
CH471067 Genomic DNA. Translation: EAW91231.1.
BC007049 mRNA. Translation: AAH07049.1.
CCDSiCCDS1377.1. [P41220-1]
PIRiI53020.
RefSeqiNP_002914.1. NM_002923.3. [P41220-1]
UniGeneiHs.78944.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AF0X-ray2.30A71-203[»]
2V4ZX-ray2.80B71-209[»]
4EKCX-ray7.40B/D72-203[»]
4EKDX-ray2.71B72-203[»]
ProteinModelPortaliP41220.
SMRiP41220.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111929. 67 interactors.
DIPiDIP-44289N.
IntActiP41220. 47 interactors.
MINTiMINT-1380095.
STRINGi9606.ENSP00000235382.

PTM databases

iPTMnetiP41220.
PhosphoSitePlusiP41220.
SwissPalmiP41220.

Polymorphism and mutation databases

BioMutaiRGS2.
DMDMi729545.

Proteomic databases

MaxQBiP41220.
PaxDbiP41220.
PeptideAtlasiP41220.
PRIDEiP41220.

Protocols and materials databases

DNASUi5997.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000235382; ENSP00000235382; ENSG00000116741. [P41220-1]
GeneIDi5997.
KEGGihsa:5997.
UCSCiuc001gsl.4. human. [P41220-1]

Organism-specific databases

CTDi5997.
DisGeNETi5997.
GeneCardsiRGS2.
HGNCiHGNC:9998. RGS2.
HPAiHPA013385.
MalaCardsiRGS2.
MIMi600861. gene.
neXtProtiNX_P41220.
OpenTargetsiENSG00000116741.
PharmGKBiPA34372.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3589. Eukaryota.
ENOG410YMJD. LUCA.
GeneTreeiENSGT00760000118903.
HOGENOMiHOG000233512.
HOVERGENiHBG013233.
InParanoidiP41220.
KOiK18154.
OMAiAVQHDCV.
OrthoDBiEOG091G0BDG.
PhylomeDBiP41220.
TreeFamiTF315837.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000116741-MONOMER.
ReactomeiR-HSA-416476. G alpha (q) signalling events.
SIGNORiP41220.

Miscellaneous databases

ChiTaRSiRGS2. human.
EvolutionaryTraceiP41220.
GeneWikiiRGS2.
GenomeRNAii5997.
PROiP41220.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000116741.
CleanExiHS_RGS2.
ExpressionAtlasiP41220. baseline and differential.
GenevisibleiP41220. HS.

Family and domain databases

Gene3Di1.10.196.10. 2 hits.
InterProiIPR016137. RGS.
IPR024066. RGS_subdom1.
[Graphical view]
PfamiPF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR01301. RGSPROTEIN.
SMARTiSM00315. RGS. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
PROSITEiPS50132. RGS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRGS2_HUMAN
AccessioniPrimary (citable) accession number: P41220
Secondary accession number(s): Q6I9U5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 2, 2016
This is version 163 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.