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P41216

- ACSL1_MOUSE

UniProt

P41216 - ACSL1_MOUSE

Protein

Long-chain-fatty-acid--CoA ligase 1

Gene

Acsl1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 2 (28 Jun 2011)
      Previous versions | rss
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    Functioni

    Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses oleate, arachidonate, eicosapentaenoate and docosahexaenoate as substrates By similarity.By similarity

    Catalytic activityi

    ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.

    Cofactori

    Magnesium.

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. long-chain fatty acid-CoA ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. adiponectin-activated signaling pathway Source: MGI
    2. lipid biosynthetic process Source: Ensembl
    3. long-chain fatty acid import Source: Ensembl
    4. positive regulation of protein serine/threonine kinase activity Source: MGI
    5. response to drug Source: Ensembl
    6. response to nutrient Source: Ensembl
    7. response to oleic acid Source: Ensembl
    8. response to organic cyclic compound Source: Ensembl
    9. triglyceride metabolic process Source: Ensembl
    10. xenobiotic catabolic process Source: Ensembl

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Fatty acid metabolism, Lipid metabolism

    Keywords - Ligandi

    ATP-binding, Magnesium, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi6.2.1.3. 3474.
    ReactomeiREACT_196530. Linoleic acid (LA) metabolism.
    REACT_196541. alpha-linolenic acid (ALA) metabolism.
    REACT_198602. PPARA activates gene expression.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Long-chain-fatty-acid--CoA ligase 1 (EC:6.2.1.3)
    Alternative name(s):
    Long-chain acyl-CoA synthetase 1
    Short name:
    LACS 1
    Gene namesi
    Name:Acsl1
    Synonyms:Acsl2, Facl2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 8

    Organism-specific databases

    MGIiMGI:102797. Acsl1.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW
    3. mitochondrial outer membrane Source: UniProtKB-SubCell
    4. mitochondrion Source: MGI
    5. peroxisomal membrane Source: UniProtKB-SubCell
    6. plasma membrane Source: Ensembl

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Mitochondrion outer membrane, Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 699699Long-chain-fatty-acid--CoA ligase 1PRO_0000193105Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine1 Publication
    Modified residuei9 – 91Nitrated tyrosineBy similarity
    Modified residuei85 – 851PhosphotyrosineBy similarity
    Modified residuei86 – 861Nitrated tyrosineBy similarity
    Glycosylationi136 – 1361O-linked (GlcNAc)By similarity
    Modified residuei208 – 2081N6-acetyllysine1 Publication
    Modified residuei357 – 3571N6-acetyllysine1 Publication
    Modified residuei387 – 3871N6-acetyllysine1 Publication
    Modified residuei621 – 6211Phosphoserine1 Publication
    Modified residuei633 – 6331N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation, Glycoprotein, Nitration, Phosphoprotein

    Proteomic databases

    MaxQBiP41216.
    PaxDbiP41216.
    PRIDEiP41216.

    PTM databases

    PhosphoSiteiP41216.

    Expressioni

    Gene expression databases

    ArrayExpressiP41216.
    BgeeiP41216.
    CleanExiMM_ACSL1.
    GenevestigatoriP41216.

    Interactioni

    Protein-protein interaction databases

    IntActiP41216. 14 interactions.
    MINTiMINT-1859502.

    Structurei

    3D structure databases

    ProteinModelPortaliP41216.
    SMRiP41216. Positions 112-639.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini46 – 699654CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei25 – 4521Helical; Signal-anchor for type III membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1022.
    GeneTreeiENSGT00690000101725.
    HOGENOMiHOG000159459.
    HOVERGENiHBG050452.
    InParanoidiQ6GTG6.
    KOiK01897.
    OMAiQATNKHA.
    OrthoDBiEOG71CFKN.
    TreeFamiTF313877.

    Family and domain databases

    InterProiIPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view]
    PfamiPF00501. AMP-binding. 1 hit.
    [Graphical view]
    PROSITEiPS00455. AMP_BINDING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P41216-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEVHELFRYF RMPELIDIRQ YVRTLPTNTL MGFGAFAALT TFWYATRPKA    50
    LKPPCDLSMQ SVEIAGTTDG IRRSAVLEDD KLLVYYYDDV RTMYDGFQRG 100
    IQVSNNGPCL GSRKPNQPYE WISYKEVAEL AECIGSGLIQ KGFKPCSEQF 150
    IGLFSQNRPE WVIVEQGCFS YSMVVVPLYD TLGADAITYI VNKAELSVIF 200
    ADKPEKAKLL LEGVENKLTP CLKIIVIMDS YGSDLVERGK KCGVEIISLK 250
    ALEDLGRVNR VKPKPPEPED LAIICFTSGT TGNPKGAMIT HQNIINDCSG 300
    FIKATESAFI ASTDDVLISF LPLAHMFETV VECVMLCHGA KIGFFQGDIR 350
    LLMDDLKVLQ PTIFPVVPRL LNRMFDRIFG QANTSLKRWL LDFASKRKEA 400
    ELRSGIVRNN SLWDKLIFHK IQSSLGGKVR LMITGAAPVS ATVLTFLRTA 450
    LGCQFYEGYG QTECTAGCCL SLPGDWTAGH VGAPMPCNYV KLVDVEEMNY 500
    LASKGEGEVC VKGANVFKGY LKDPARTAEA LDKDGWLHTG DIGKWLPNGT 550
    LKIIDRKKHI FKLAQGEYIA PEKIENIYLR SEAVAQVFVH GESLQAFLIA 600
    VVVPDVESLP SWAQKRGLQG SFEELCRNKD INKAILDDLL KLGKEAGLKP 650
    FEQVKGIAVH PELFSIDNGL LTPTLKAKRP ELRNYFRSQI DELYATIKI 699
    Length:699
    Mass (Da):77,951
    Last modified:June 28, 2011 - v2
    Checksum:iC8319972A764CCFA
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti401 – 4022EL → DV in AAA52193. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U15977 mRNA. Translation: AAA52193.1.
    AK004897 mRNA. Translation: BAB23652.1.
    AK145900 mRNA. Translation: BAE26736.1.
    AK149406 mRNA. Translation: BAE28854.1.
    AK152772 mRNA. Translation: BAE31484.1.
    AK153050 mRNA. Translation: BAE31678.1.
    AK161189 mRNA. Translation: BAE36230.1.
    AK168078 mRNA. Translation: BAE40051.1.
    BC056644 mRNA. Translation: AAH56644.1.
    CCDSiCCDS22291.1.
    RefSeqiNP_032007.2. NM_007981.3.
    XP_006509326.1. XM_006509263.1.
    XP_006509327.1. XM_006509264.1.
    XP_006509328.1. XM_006509265.1.
    XP_006509329.1. XM_006509266.1.
    UniGeneiMm.210323.

    Genome annotation databases

    EnsembliENSMUST00000034046; ENSMUSP00000034046; ENSMUSG00000018796.
    ENSMUST00000110372; ENSMUSP00000106001; ENSMUSG00000018796.
    GeneIDi14081.
    KEGGimmu:14081.
    UCSCiuc009lqe.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U15977 mRNA. Translation: AAA52193.1 .
    AK004897 mRNA. Translation: BAB23652.1 .
    AK145900 mRNA. Translation: BAE26736.1 .
    AK149406 mRNA. Translation: BAE28854.1 .
    AK152772 mRNA. Translation: BAE31484.1 .
    AK153050 mRNA. Translation: BAE31678.1 .
    AK161189 mRNA. Translation: BAE36230.1 .
    AK168078 mRNA. Translation: BAE40051.1 .
    BC056644 mRNA. Translation: AAH56644.1 .
    CCDSi CCDS22291.1.
    RefSeqi NP_032007.2. NM_007981.3.
    XP_006509326.1. XM_006509263.1.
    XP_006509327.1. XM_006509264.1.
    XP_006509328.1. XM_006509265.1.
    XP_006509329.1. XM_006509266.1.
    UniGenei Mm.210323.

    3D structure databases

    ProteinModelPortali P41216.
    SMRi P41216. Positions 112-639.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P41216. 14 interactions.
    MINTi MINT-1859502.

    PTM databases

    PhosphoSitei P41216.

    Proteomic databases

    MaxQBi P41216.
    PaxDbi P41216.
    PRIDEi P41216.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000034046 ; ENSMUSP00000034046 ; ENSMUSG00000018796 .
    ENSMUST00000110372 ; ENSMUSP00000106001 ; ENSMUSG00000018796 .
    GeneIDi 14081.
    KEGGi mmu:14081.
    UCSCi uc009lqe.1. mouse.

    Organism-specific databases

    CTDi 2180.
    MGIi MGI:102797. Acsl1.

    Phylogenomic databases

    eggNOGi COG1022.
    GeneTreei ENSGT00690000101725.
    HOGENOMi HOG000159459.
    HOVERGENi HBG050452.
    InParanoidi Q6GTG6.
    KOi K01897.
    OMAi QATNKHA.
    OrthoDBi EOG71CFKN.
    TreeFami TF313877.

    Enzyme and pathway databases

    BRENDAi 6.2.1.3. 3474.
    Reactomei REACT_196530. Linoleic acid (LA) metabolism.
    REACT_196541. alpha-linolenic acid (ALA) metabolism.
    REACT_198602. PPARA activates gene expression.

    Miscellaneous databases

    NextBioi 285092.
    PROi P41216.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P41216.
    Bgeei P41216.
    CleanExi MM_ACSL1.
    Genevestigatori P41216.

    Family and domain databases

    InterProi IPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view ]
    Pfami PF00501. AMP-binding. 1 hit.
    [Graphical view ]
    PROSITEi PS00455. AMP_BINDING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Schaffer J.E.
      Submitted (OCT-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Swiss.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: BALB/c and C57BL/6J.
      Tissue: Liver.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Liver.
    4. Bienvenut W.V.
      Submitted (JUL-2005) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 1-8; 358-369; 389-396; 563-573; 617-627 AND 634-641, ACETYLATION AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6.
      Tissue: Liver.
    5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-621, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    6. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
      Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
      Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-208; LYS-357 AND LYS-387, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiACSL1_MOUSE
    AccessioniPrimary (citable) accession number: P41216
    Secondary accession number(s): Q6GTG6, Q9DBK5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: June 28, 2011
    Last modified: October 1, 2014
    This is version 125 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3