Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Long-chain-fatty-acid--CoA ligase 1

Gene

Acsl1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Activation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses oleate, arachidonate, eicosapentaenoate and docosahexaenoate as substrates (By similarity).By similarity

Catalytic activityi

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.

Cofactori

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processFatty acid metabolism, Lipid metabolism
LigandATP-binding, Magnesium, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.2.1.3 3474
ReactomeiR-MMU-2046105 Linoleic acid (LA) metabolism
R-MMU-2046106 alpha-linolenic acid (ALA) metabolism
R-MMU-75876 Synthesis of very long-chain fatty acyl-CoAs

Names & Taxonomyi

Protein namesi
Recommended name:
Long-chain-fatty-acid--CoA ligase 1 (EC:6.2.1.3)
Alternative name(s):
Long-chain acyl-CoA synthetase 1
Short name:
LACS 1
Gene namesi
Name:Acsl1
Synonyms:Acsl2, Facl2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:102797 Acsl1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei25 – 45Helical; Signal-anchor for type III membrane proteinSequence analysisAdd BLAST21
Topological domaini46 – 699CytoplasmicSequence analysisAdd BLAST654

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome, Mitochondrion, Mitochondrion outer membrane, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001931051 – 699Long-chain-fatty-acid--CoA ligase 1Add BLAST699

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionine1 Publication1
Modified residuei9Nitrated tyrosineBy similarity1
Modified residuei85PhosphotyrosineBy similarity1
Modified residuei86Nitrated tyrosineBy similarity1
Glycosylationi136O-linked (GlcNAc) serineBy similarity1
Modified residuei208N6-acetyllysineCombined sources1
Modified residuei357N6-acetyllysineCombined sources1
Modified residuei387N6-acetyllysineCombined sources1
Modified residuei621PhosphoserineCombined sources1
Modified residuei633N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Glycoprotein, Nitration, Phosphoprotein

Proteomic databases

EPDiP41216
MaxQBiP41216
PaxDbiP41216
PRIDEiP41216

PTM databases

CarbonylDBiP41216
iPTMnetiP41216
PhosphoSitePlusiP41216
SwissPalmiP41216

Expressioni

Gene expression databases

BgeeiENSMUSG00000018796
CleanExiMM_ACSL1
ExpressionAtlasiP41216 baseline and differential
GenevisibleiP41216 MM

Interactioni

Protein-protein interaction databases

BioGridi199585, 1 interactor
IntActiP41216, 14 interactors
MINTiP41216
STRINGi10090.ENSMUSP00000034046

Structurei

3D structure databases

ProteinModelPortaliP41216
SMRiP41216
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1256 Eukaryota
COG1022 LUCA
GeneTreeiENSGT00690000101725
HOGENOMiHOG000159459
HOVERGENiHBG050452
InParanoidiP41216
KOiK01897
OMAiECVMLCH
TreeFamiTF313877

Family and domain databases

InterProiView protein in InterPro
IPR025110 AMP-bd_C
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
PfamiView protein in Pfam
PF00501 AMP-binding, 1 hit
PF13193 AMP-binding_C, 1 hit
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 1 hit

Sequencei

Sequence statusi: Complete.

P41216-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVHELFRYF RMPELIDIRQ YVRTLPTNTL MGFGAFAALT TFWYATRPKA
60 70 80 90 100
LKPPCDLSMQ SVEIAGTTDG IRRSAVLEDD KLLVYYYDDV RTMYDGFQRG
110 120 130 140 150
IQVSNNGPCL GSRKPNQPYE WISYKEVAEL AECIGSGLIQ KGFKPCSEQF
160 170 180 190 200
IGLFSQNRPE WVIVEQGCFS YSMVVVPLYD TLGADAITYI VNKAELSVIF
210 220 230 240 250
ADKPEKAKLL LEGVENKLTP CLKIIVIMDS YGSDLVERGK KCGVEIISLK
260 270 280 290 300
ALEDLGRVNR VKPKPPEPED LAIICFTSGT TGNPKGAMIT HQNIINDCSG
310 320 330 340 350
FIKATESAFI ASTDDVLISF LPLAHMFETV VECVMLCHGA KIGFFQGDIR
360 370 380 390 400
LLMDDLKVLQ PTIFPVVPRL LNRMFDRIFG QANTSLKRWL LDFASKRKEA
410 420 430 440 450
ELRSGIVRNN SLWDKLIFHK IQSSLGGKVR LMITGAAPVS ATVLTFLRTA
460 470 480 490 500
LGCQFYEGYG QTECTAGCCL SLPGDWTAGH VGAPMPCNYV KLVDVEEMNY
510 520 530 540 550
LASKGEGEVC VKGANVFKGY LKDPARTAEA LDKDGWLHTG DIGKWLPNGT
560 570 580 590 600
LKIIDRKKHI FKLAQGEYIA PEKIENIYLR SEAVAQVFVH GESLQAFLIA
610 620 630 640 650
VVVPDVESLP SWAQKRGLQG SFEELCRNKD INKAILDDLL KLGKEAGLKP
660 670 680 690
FEQVKGIAVH PELFSIDNGL LTPTLKAKRP ELRNYFRSQI DELYATIKI
Length:699
Mass (Da):77,951
Last modified:June 28, 2011 - v2
Checksum:iC8319972A764CCFA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti401 – 402EL → DV in AAA52193 (Ref. 1) Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15977 mRNA Translation: AAA52193.1
AK004897 mRNA Translation: BAB23652.1
AK145900 mRNA Translation: BAE26736.1
AK149406 mRNA Translation: BAE28854.1
AK152772 mRNA Translation: BAE31484.1
AK153050 mRNA Translation: BAE31678.1
AK161189 mRNA Translation: BAE36230.1
AK168078 mRNA Translation: BAE40051.1
BC056644 mRNA Translation: AAH56644.1
CCDSiCCDS22291.1
RefSeqiNP_001289092.1, NM_001302163.1
NP_032007.2, NM_007981.4
XP_006509325.2, XM_006509262.3
XP_006509327.1, XM_006509264.3
XP_006509328.1, XM_006509265.2
XP_006509329.1, XM_006509266.2
XP_017168052.1, XM_017312563.1
UniGeneiMm.210323

Genome annotation databases

EnsembliENSMUST00000034046; ENSMUSP00000034046; ENSMUSG00000018796
ENSMUST00000110372; ENSMUSP00000106001; ENSMUSG00000018796
GeneIDi14081
KEGGimmu:14081
UCSCiuc009lqe.2 mouse

Similar proteinsi

Entry informationi

Entry nameiACSL1_MOUSE
AccessioniPrimary (citable) accession number: P41216
Secondary accession number(s): Q6GTG6, Q9DBK5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: June 28, 2011
Last modified: April 25, 2018
This is version 150 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health