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Protein

Eukaryotic translation initiation factor 2D

Gene

EIF2D

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Translation initiation factor that is able to deliver tRNA to the P-site of the eukaryotic ribosome in a GTP-independent manner. The binding of Met-tRNA(I) occurs after the AUG codon finds its position in the P-site of 40S ribosomes, the situation that takes place during initiation complex formation on some specific RNAs. Its activity in tRNA binding with 40S subunits does not require the presence of the aminoacyl moiety. Possesses the unique ability to deliver non-Met (elongator) tRNAs into the P-site of the 40S subunit. In addition to its role in initiation, can promote release of deacylated tRNA and mRNA from recycled 40S subunits following ABCE1-mediated dissociation of post-termination ribosomal complexes into subunits.2 Publications

GO - Molecular functioni

  • receptor activity Source: ProtInc
  • translation initiation factor activity Source: UniProtKB

GO - Biological processi

  • formation of translation preinitiation complex Source: UniProtKB
  • intracellular protein transport Source: ProtInc
  • IRES-dependent translational initiation Source: UniProtKB
  • ribosome disassembly Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Enzyme and pathway databases

BioCyciZFISH:ENSG00000143486-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 2D
Short name:
eIF2d
Alternative name(s):
Hepatocellular carcinoma-associated antigen 56
Ligatin
Gene namesi
Name:EIF2D
Synonyms:HCA56, LGTN
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:6583. EIF2D.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • nucleus Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000143486.
PharmGKBiPA30355.

Polymorphism and mutation databases

BioMutaiEIF2D.
DMDMi158957575.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001306111 – 584Eukaryotic translation initiation factor 2DAdd BLAST584

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei237PhosphoserineCombined sources1
Modified residuei254PhosphoserineBy similarity1
Modified residuei361PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP41214.
MaxQBiP41214.
PaxDbiP41214.
PeptideAtlasiP41214.
PRIDEiP41214.

PTM databases

iPTMnetiP41214.
PhosphoSitePlusiP41214.

Expressioni

Developmental stagei

Found during embryonic development and in early differentiated states.

Gene expression databases

BgeeiENSG00000143486.
ExpressionAtlasiP41214. baseline and differential.
GenevisibleiP41214. HS.

Organism-specific databases

HPAiHPA028220.

Interactioni

Protein-protein interaction databases

BioGridi108259. 22 interactors.
IntActiP41214. 1 interactor.
MINTiMINT-3015310.
STRINGi9606.ENSP00000271764.

Structurei

3D structure databases

ProteinModelPortaliP41214.
SMRiP41214.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini93 – 173PUAPROSITE-ProRule annotationAdd BLAST81
Domaini491 – 564SUI1PROSITE-ProRule annotationAdd BLAST74

Sequence similaritiesi

Belongs to the eIF2D family.Curated
Contains 1 PUA domain.PROSITE-ProRule annotation
Contains 1 SUI1 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2522. Eukaryota.
ENOG410XSAV. LUCA.
GeneTreeiENSGT00550000074865.
HOVERGENiHBG006268.
InParanoidiP41214.
KOiK15027.
OMAiTVMKLPW.
OrthoDBiEOG091G0DTH.
PhylomeDBiP41214.
TreeFamiTF105830.

Family and domain databases

Gene3Di1.10.245.10. 1 hit.
2.30.130.10. 1 hit.
3.30.780.10. 1 hit.
InterProiIPR002478. PUA.
IPR015947. PUA-like_domain.
IPR001950. SUI1.
IPR003121. SWIB_MDM2_domain.
[Graphical view]
PfamiPF01253. SUI1. 1 hit.
[Graphical view]
SMARTiSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF47592. SSF47592. 1 hit.
SSF55159. SSF55159. 1 hit.
SSF88697. SSF88697. 1 hit.
PROSITEiPS50890. PUA. 1 hit.
PS50296. SUI1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P41214-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MFAKAFRVKS NTAIKGSDRR KLRADVTTAF PTLGTDQVSE LVPGKEELNI
60 70 80 90 100
VKLYAHKGDA VTVYVSGGNP ILFELEKNLY PTVYTLWSYP DLLPTFTTWP
110 120 130 140 150
LVLEKLVGGA DLMLPGLVMP PAGLPQVQKG DLCAISLVGN RAPVAIGVAA
160 170 180 190 200
MSTAEMLTSG LKGRGFSVLH TYQDHLWRSG NKSSPPSIAP LALDSADLSE
210 220 230 240 250
EKGSVQMDST LQGDMRHMTL EGEEENGEVH QAREDKSLSE APEDTSTRGL
260 270 280 290 300
NQDSTDSKTL QEQMDELLQQ CFLHALKCRV KKADLPLLTS TFLGSHMFSC
310 320 330 340 350
CPEGRQLDIK KSSYKKLSKF LQQMQQEQII QVKELSKGVE SIVAVDWKHP
360 370 380 390 400
RITSFVIPEP SPTSQTIQEG SREQPYHPPD IKPLYCVPAS MTLLFQESGH
410 420 430 440 450
KKGSFLEGSE VRTIVINYAK KNDLVDADNK NLVRLDPILC DCILEKNEQH
460 470 480 490 500
TVMKLPWDSL LTRCLEKLQP AYQVTLPGQE PIVKKGRICP IDITLAQRAS
510 520 530 540 550
NKKVTVVRNL EAYGLDPYSV AAILQQRCQA STTVNPAPGA KDSLQVQIQG
560 570 580
NQVHHLGWLL LEEYQLPRKH IQGLEKALKP GKKK
Length:584
Mass (Da):64,706
Last modified:August 21, 2007 - v3
Checksum:iC302B63268231154
GO
Isoform 2 (identifier: P41214-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     177-300: Missing.

Show »
Length:460
Mass (Da):51,013
Checksum:i6ED8D2011C9ECB29
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti11N → D in BAA91942 (PubMed:14702039).Curated1
Sequence conflicti66S → N in AAF34185 (PubMed:12097419).Curated1
Sequence conflicti66S → N in AAF74205 (PubMed:12097419).Curated1
Sequence conflicti210T → N in AAF34185 (PubMed:12097419).Curated1
Sequence conflicti304 – 308GRQLD → DDNWT in AAD41909 (PubMed:2482295).Curated5

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_052507210T → I.Corresponds to variant rs35252702dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_006300177 – 300Missing in isoform 2. 1 PublicationAdd BLAST124

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF220417 mRNA. Translation: AAF34185.2.
AF262403 mRNA. Translation: AAF74205.1.
AK001852 mRNA. Translation: BAA91942.1.
AL591846 Genomic DNA. Translation: CAI13533.1.
AL591846 Genomic DNA. Translation: CAI13534.1.
CH471100 Genomic DNA. Translation: EAW93536.1.
CH471100 Genomic DNA. Translation: EAW93540.1.
BC001585 mRNA. Translation: AAH01585.1.
BC039134 mRNA. Translation: AAH39134.2.
BC058905 mRNA. Translation: AAH58905.1.
AL162001 mRNA. Translation: CAB82330.1.
AF159586 mRNA. Translation: AAD41909.1.
CCDSiCCDS1465.1. [P41214-1]
CCDS55680.1. [P41214-2]
PIRiA60697.
T47178.
RefSeqiNP_001188407.1. NM_001201478.1. [P41214-2]
NP_008824.2. NM_006893.2. [P41214-1]
UniGeneiHs.497581.

Genome annotation databases

EnsembliENST00000271764; ENSP00000271764; ENSG00000143486. [P41214-1]
ENST00000367114; ENSP00000356081; ENSG00000143486. [P41214-2]
GeneIDi1939.
KEGGihsa:1939.
UCSCiuc001heh.4. human. [P41214-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF220417 mRNA. Translation: AAF34185.2.
AF262403 mRNA. Translation: AAF74205.1.
AK001852 mRNA. Translation: BAA91942.1.
AL591846 Genomic DNA. Translation: CAI13533.1.
AL591846 Genomic DNA. Translation: CAI13534.1.
CH471100 Genomic DNA. Translation: EAW93536.1.
CH471100 Genomic DNA. Translation: EAW93540.1.
BC001585 mRNA. Translation: AAH01585.1.
BC039134 mRNA. Translation: AAH39134.2.
BC058905 mRNA. Translation: AAH58905.1.
AL162001 mRNA. Translation: CAB82330.1.
AF159586 mRNA. Translation: AAD41909.1.
CCDSiCCDS1465.1. [P41214-1]
CCDS55680.1. [P41214-2]
PIRiA60697.
T47178.
RefSeqiNP_001188407.1. NM_001201478.1. [P41214-2]
NP_008824.2. NM_006893.2. [P41214-1]
UniGeneiHs.497581.

3D structure databases

ProteinModelPortaliP41214.
SMRiP41214.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108259. 22 interactors.
IntActiP41214. 1 interactor.
MINTiMINT-3015310.
STRINGi9606.ENSP00000271764.

PTM databases

iPTMnetiP41214.
PhosphoSitePlusiP41214.

Polymorphism and mutation databases

BioMutaiEIF2D.
DMDMi158957575.

Proteomic databases

EPDiP41214.
MaxQBiP41214.
PaxDbiP41214.
PeptideAtlasiP41214.
PRIDEiP41214.

Protocols and materials databases

DNASUi1939.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000271764; ENSP00000271764; ENSG00000143486. [P41214-1]
ENST00000367114; ENSP00000356081; ENSG00000143486. [P41214-2]
GeneIDi1939.
KEGGihsa:1939.
UCSCiuc001heh.4. human. [P41214-1]

Organism-specific databases

CTDi1939.
GeneCardsiEIF2D.
HGNCiHGNC:6583. EIF2D.
HPAiHPA028220.
MIMi613709. gene.
neXtProtiNX_P41214.
OpenTargetsiENSG00000143486.
PharmGKBiPA30355.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2522. Eukaryota.
ENOG410XSAV. LUCA.
GeneTreeiENSGT00550000074865.
HOVERGENiHBG006268.
InParanoidiP41214.
KOiK15027.
OMAiTVMKLPW.
OrthoDBiEOG091G0DTH.
PhylomeDBiP41214.
TreeFamiTF105830.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000143486-MONOMER.

Miscellaneous databases

ChiTaRSiEIF2D. human.
GeneWikiiLGTN.
GenomeRNAii1939.
PROiP41214.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000143486.
ExpressionAtlasiP41214. baseline and differential.
GenevisibleiP41214. HS.

Family and domain databases

Gene3Di1.10.245.10. 1 hit.
2.30.130.10. 1 hit.
3.30.780.10. 1 hit.
InterProiIPR002478. PUA.
IPR015947. PUA-like_domain.
IPR001950. SUI1.
IPR003121. SWIB_MDM2_domain.
[Graphical view]
PfamiPF01253. SUI1. 1 hit.
[Graphical view]
SMARTiSM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF47592. SSF47592. 1 hit.
SSF55159. SSF55159. 1 hit.
SSF88697. SSF88697. 1 hit.
PROSITEiPS50890. PUA. 1 hit.
PS50296. SUI1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEIF2D_HUMAN
AccessioniPrimary (citable) accession number: P41214
Secondary accession number(s): Q5SY40
, Q8IXV3, Q96DG3, Q96TG7, Q9NR27, Q9NSN0, Q9NV18, Q9NZ21
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: August 21, 2007
Last modified: November 30, 2016
This is version 153 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Was previously erroneously called ligatin, a trafficking receptor for phosphoglycoproteins, while ligatin is actually a distinct 10 kDa filamentous membrane protein encoded by a still unidentified gene.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.