Reviewed, UniProtKB/Swiss-Prot P41213 (LDOX_MAIZE)

Names and origin

Protein names

Recommended name:
    Leucoanthocyanidin dioxygenase
      Short name=LDOX
      Short name=Leucocyanidin oxygenase
    EC=1.14.11.19
Alternative name(s):
    Leucoanthocyanidin hydroxylase

Gene names

Name:

A2

Organism

Zea mays (Maize)

Taxonomic identifier

4577 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › PACCAD clade › Panicoideae › Andropogoneae › Zea

Protein attributes

Sequence length	395 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Oxidation of leucoanthocyanidins into anthocyanidins.
Catalytic activity	Leucocyanidin + 2-oxoglutarate + O(2) = cis- and trans-dihydroquercetins + succinate + CO(2) + H(2)O.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 ascorbate molecule per subunit By similarity.
Pathway	Pigment biosynthesis; anthocyanin biosynthesis.
Sequence similarities	Belongs to the iron/ascorbate-dependent oxidoreductase family.

Ontologies

Keywords
Biological process	Flavonoid biosynthesis
Ligand	Iron Metal-binding Vitamin C
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	flavonoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-ascorbic acid binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW leucocyanidin oxygenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 395	395	Leucoanthocyanidin dioxygenase		PRO_0000067300
Sites
	Metal binding	254	1	Iron By similarity
	Metal binding	256	1	Iron By similarity
	Metal binding	310	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	P41213-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: C43B91DDC6FC7F1A	FASTA	395	43,356
	10 20 30 40 50 60 MESSPLLQLP AARVEALSLS GLSAIPPEYV RPADERAGLG DAFDLARTHA NDHTAPRIPV 70 80 90 100 110 120 VDISPFLDSS SQQQQRDECV EAVRAAAADW GVMHIAGHGI PAELMDRLRA AGTAFFALPV 130 140 150 160 170 180 QDKEAYANDP AAGRLQGYGS RLATNTCGQR EWEDYLFHLV HPDGLADHAL WPAYPPDYIA 190 200 210 220 230 240 ATRDFGRRTR DLASTLLAIL SMGLLGTDRG DALEKALTTT TTRTAADDDL LLQLKINYYP 250 260 270 280 290 300 RCPQPELAVG VEAHTDVSAL SFILHNGVPG LQVLHGARWV TARHEPGTII VHVGDALEIL 310 320 330 340 350 360 SNGRYTSVLH RGLVNREAVR ISWVVFCEPP PDSVLLHPLP ELVTEGHPAR FTPRTFKQHL 370 380 390 DRKLFKKKQQ HKAKAEEEDG GNGDHHRHEP PPQTN

References

[1]

"The En/Spm transposable element of Zea mays contains splice sites at the termini generating a novel intron from a dSpm element in the A2 gene." Menssen A., Hoehmann S., Martin W., Schnable P.S., Peterson P.S., Saedler H., Gierl A. EMBO J. 9:3051-3057(1990) [PubMed: 2170105] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X55314 mRNA. Translation: CAA39022.1.
PIR	S12043.
RefSeq	NP_001106074.1.
UniGene	Zm.62
3D structure databases
HSSP	HSSP built from PDB template 1GP6 based on UniProtKB Q96323.
ModBase	Search...
Genome annotation databases
GeneID	100127010.
Organism-specific databases
Gramene	P41213.
MaizeGDB	99960.
Family and domain databases
InterPro	IPR005123. 2OG-FeII_Oase. [Graphical view]
Pfam	PF03171. 2OG-FeII_Oxy. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LDOX_MAIZE

Accession

Primary (citable) accession number: P41213

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 4, 2008
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)