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Protein

Centrin-2

Gene

CETN2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a fundamental role in microtubule organizing center structure and function. Required for centriole duplication and correct spindle formation. Has a role in regulating cytokinesis and genome stability via cooperation with CALM1 and CCP110.
Involved in global genome nucleotide excision repair (GG-NER) by acting as component of the XPC complex. Cooperatively with RAD23B appears to stabilize XPC. In vitro, stimulates DNA binding of the XPC:RAD23B dimer.
The XPC complex is proposed to represent the first factor bound at the sites of DNA damage and together with other core recognition factors, XPA, RPA and the TFIIH complex, is part of the pre-incision (or initial recognition) complex. The XPC complex recognizes a wide spectrum of damaged DNA characterized by distortions of the DNA helix such as single-stranded loops, mismatched bubbles or single-stranded overhangs. The orientation of XPC complex binding appears to be crucial for inducing a productive NER. XPC complex is proposed to recognize and to interact with unpaired bases on the undamaged DNA strand which is followed by recruitment of the TFIIH complex and subsequent scanning for lesions in the opposite strand in a 5'-to-3' direction by the NER machinery. Cyclobutane pyrimidine dimers (CPDs) which are formed upon UV-induced DNA damage esacpe detection by the XPC complex due to a low degree of structural perurbation. Instead they are detected by the UV-DDB complex which in turn recruits and cooperates with the XPC complex in the respective DNA repair.
Component of the TREX-2 complex (transcription and export complex 2), composed of at least ENY2, GANP, PCID2, DSS1, and either centrin CETN2 or CETN3 (PubMed:22307388). The TREX-2 complex functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket). TREX-2 participates in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery (PubMed:22307388).1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi114 – 1251Add BLAST12
Calcium bindingi150 – 1612Add BLAST12

GO - Molecular functioni

  • calcium ion binding Source: UniProtKB

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • centriole replication Source: UniProtKB
  • G2/M transition of mitotic cell cycle Source: Reactome
  • global genome nucleotide-excision repair Source: Reactome
  • mitotic nuclear division Source: UniProtKB
  • nucleotide-excision repair Source: UniProtKB
  • nucleotide-excision repair, DNA damage recognition Source: Reactome
  • nucleotide-excision repair, DNA duplex unwinding Source: Reactome
  • nucleotide-excision repair, preincision complex assembly Source: Reactome
  • protein sumoylation Source: Reactome
  • regulation of cytokinesis Source: UniProtKB
  • spermatogenesis Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, DNA damage, DNA repair, Mitosis

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000147400-MONOMER.
ReactomeiR-HSA-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-HSA-3108214. SUMOylation of DNA damage response and repair proteins.
R-HSA-380259. Loss of Nlp from mitotic centrosomes.
R-HSA-380270. Recruitment of mitotic centrosome proteins and complexes.
R-HSA-5620912. Anchoring of the basal body to the plasma membrane.
R-HSA-5696394. DNA Damage Recognition in GG-NER.
R-HSA-5696395. Formation of Incision Complex in GG-NER.
R-HSA-8854518. AURKA Activation by TPX2.
SIGNORiP41208.

Names & Taxonomyi

Protein namesi
Recommended name:
Centrin-2
Alternative name(s):
Caltractin isoform 1
Gene namesi
Name:CETN2
Synonyms:CALT, CEN2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

HGNCiHGNC:1867. CETN2.

Subcellular locationi

GO - Cellular componenti

  • centriole Source: UniProtKB
  • centrosome Source: UniProtKB
  • ciliary basal body Source: Ensembl
  • cytosol Source: Reactome
  • intracellular Source: LIFEdb
  • nucleoplasm Source: Reactome
  • photoreceptor connecting cilium Source: Ensembl
  • XPC complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi1069.
OpenTargetsiENSG00000147400.
PharmGKBiPA26420.

Polymorphism and mutation databases

BioMutaiCETN2.
DMDMi729052.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000735612 – 172Centrin-2Add BLAST171

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei20PhosphoserineCombined sources1
Modified residuei26PhosphothreonineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP41208.
MaxQBiP41208.
PaxDbiP41208.
PeptideAtlasiP41208.
PRIDEiP41208.
TopDownProteomicsiP41208.

PTM databases

iPTMnetiP41208.
PhosphoSitePlusiP41208.

Expressioni

Gene expression databases

BgeeiENSG00000147400.
CleanExiHS_CETN2.
GenevisibleiP41208. HS.

Organism-specific databases

HPAiHPA028956.

Interactioni

Subunit structurei

Monomer. Homooligomer. Interacts with CCP110, SFI1. Component of the XPC complex composed of XPC, RAD23B and CETN2. Component of the TREX-2 complex (transcription and export complex 2), composed of at least ENY2, GANP, PCID2, DSS1, and either centrin CETN2 or CETN3 (PubMed:22307388).8 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CCP110O433033EBI-1789926,EBI-1566217
PNMA5Q96PV43EBI-1789926,EBI-10171633
POC5Q8NA727EBI-1789926,EBI-2561090
SFI1A8K8P34EBI-1789926,EBI-743371
SGSM1Q2NKQ1-45EBI-1789926,EBI-10182463
XPCQ018314EBI-1789926,EBI-372610

Protein-protein interaction databases

BioGridi107496. 63 interactors.
IntActiP41208. 40 interactors.
MINTiMINT-3015206.
STRINGi9606.ENSP00000359300.

Structurei

Secondary structure

1172
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi22 – 24Combined sources3
Helixi27 – 38Combined sources12
Beta strandi46 – 49Combined sources4
Helixi50 – 52Combined sources3
Helixi53 – 59Combined sources7
Helixi66 – 76Combined sources11
Turni77 – 79Combined sources3
Beta strandi82 – 85Combined sources4
Helixi86 – 113Combined sources28
Beta strandi115 – 117Combined sources3
Beta strandi118 – 121Combined sources4
Helixi123 – 132Combined sources10
Helixi139 – 149Combined sources11
Beta strandi150 – 152Combined sources3
Beta strandi153 – 157Combined sources5
Helixi159 – 166Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1M39NMR-A84-172[»]
1ZMZNMR-A1-98[»]
2A4JNMR-A94-172[»]
2GGMX-ray2.35A/B1-172[»]
2K2INMR-A94-172[»]
2OBHX-ray1.80A/B26-168[»]
ProteinModelPortaliP41208.
SMRiP41208.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP41208.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 63EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini64 – 99EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini101 – 136EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini137 – 172EF-hand 4PROSITE-ProRule annotationAdd BLAST36

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 25Required for self-assemblyAdd BLAST24

Sequence similaritiesi

Belongs to the centrin family.Curated
Contains 4 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0028. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00670000097718.
HOGENOMiHOG000233018.
HOVERGENiHBG012180.
InParanoidiP41208.
KOiK10840.
OMAiMIDTKEL.
OrthoDBiEOG091G0RLZ.
PhylomeDBiP41208.
TreeFamiTF101141.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 2 hits.
InterProiIPR029528. CETN2.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000629. RNA-helicase_DEAD-box_CS.
[Graphical view]
PANTHERiPTHR23050:SF180. PTHR23050:SF180. 1 hit.
PfamiPF13499. EF-hand_7. 2 hits.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P41208-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASNFKKANM ASSSQRKRMS PKPELTEEQK QEIREAFDLF DADGTGTIDV
60 70 80 90 100
KELKVAMRAL GFEPKKEEIK KMISEIDKEG TGKMNFGDFL TVMTQKMSEK
110 120 130 140 150
DTKEEILKAF KLFDDDETGK ISFKNLKRVA KELGENLTDE ELQEMIDEAD
160 170
RDGDGEVSEQ EFLRIMKKTS LY
Length:172
Mass (Da):19,738
Last modified:February 1, 1995 - v1
Checksum:i59CFD706AD7011B5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72964 mRNA. Translation: CAA51467.1.
U82671 Genomic DNA. No translation available.
AK311940 mRNA. Translation: BAG34881.1.
BT007256 mRNA. Translation: AAP35920.1.
AY919675 Genomic DNA. Translation: AAW82436.1.
CH471172 Genomic DNA. Translation: EAW72900.1.
BC005334 mRNA. Translation: AAH05334.1.
BC013873 mRNA. Translation: AAH13873.1.
CCDSiCCDS14716.1.
PIRiA49652.
RefSeqiNP_004335.1. NM_004344.1.
UniGeneiHs.82794.

Genome annotation databases

EnsembliENST00000370277; ENSP00000359300; ENSG00000147400.
GeneIDi1069.
KEGGihsa:1069.
UCSCiuc004fgq.4. human.

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X72964 mRNA. Translation: CAA51467.1.
U82671 Genomic DNA. No translation available.
AK311940 mRNA. Translation: BAG34881.1.
BT007256 mRNA. Translation: AAP35920.1.
AY919675 Genomic DNA. Translation: AAW82436.1.
CH471172 Genomic DNA. Translation: EAW72900.1.
BC005334 mRNA. Translation: AAH05334.1.
BC013873 mRNA. Translation: AAH13873.1.
CCDSiCCDS14716.1.
PIRiA49652.
RefSeqiNP_004335.1. NM_004344.1.
UniGeneiHs.82794.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1M39NMR-A84-172[»]
1ZMZNMR-A1-98[»]
2A4JNMR-A94-172[»]
2GGMX-ray2.35A/B1-172[»]
2K2INMR-A94-172[»]
2OBHX-ray1.80A/B26-168[»]
ProteinModelPortaliP41208.
SMRiP41208.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107496. 63 interactors.
IntActiP41208. 40 interactors.
MINTiMINT-3015206.
STRINGi9606.ENSP00000359300.

PTM databases

iPTMnetiP41208.
PhosphoSitePlusiP41208.

Polymorphism and mutation databases

BioMutaiCETN2.
DMDMi729052.

Proteomic databases

EPDiP41208.
MaxQBiP41208.
PaxDbiP41208.
PeptideAtlasiP41208.
PRIDEiP41208.
TopDownProteomicsiP41208.

Protocols and materials databases

DNASUi1069.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000370277; ENSP00000359300; ENSG00000147400.
GeneIDi1069.
KEGGihsa:1069.
UCSCiuc004fgq.4. human.

Organism-specific databases

CTDi1069.
DisGeNETi1069.
GeneCardsiCETN2.
HGNCiHGNC:1867. CETN2.
HPAiHPA028956.
MIMi300006. gene.
neXtProtiNX_P41208.
OpenTargetsiENSG00000147400.
PharmGKBiPA26420.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0028. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00670000097718.
HOGENOMiHOG000233018.
HOVERGENiHBG012180.
InParanoidiP41208.
KOiK10840.
OMAiMIDTKEL.
OrthoDBiEOG091G0RLZ.
PhylomeDBiP41208.
TreeFamiTF101141.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000147400-MONOMER.
ReactomeiR-HSA-2565942. Regulation of PLK1 Activity at G2/M Transition.
R-HSA-3108214. SUMOylation of DNA damage response and repair proteins.
R-HSA-380259. Loss of Nlp from mitotic centrosomes.
R-HSA-380270. Recruitment of mitotic centrosome proteins and complexes.
R-HSA-5620912. Anchoring of the basal body to the plasma membrane.
R-HSA-5696394. DNA Damage Recognition in GG-NER.
R-HSA-5696395. Formation of Incision Complex in GG-NER.
R-HSA-8854518. AURKA Activation by TPX2.
SIGNORiP41208.

Miscellaneous databases

EvolutionaryTraceiP41208.
GeneWikiiCETN2.
GenomeRNAii1069.
PROiP41208.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000147400.
CleanExiHS_CETN2.
GenevisibleiP41208. HS.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 2 hits.
InterProiIPR029528. CETN2.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000629. RNA-helicase_DEAD-box_CS.
[Graphical view]
PANTHERiPTHR23050:SF180. PTHR23050:SF180. 1 hit.
PfamiPF13499. EF-hand_7. 2 hits.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCETN2_HUMAN
AccessioniPrimary (citable) accession number: P41208
Secondary accession number(s): B2R4T4, Q53XW1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 30, 2016
This is version 178 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Binds two moles of calcium per mole of protein.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.