P41184 (RPOB_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit beta
Short name=RNAP subunit beta
EC=2.7.7.6

Alternative name(s):
RNA polymerase subunit beta
Transcriptase subunit beta

Gene names

Name:	rpoB
Ordered Locus Names:	BUsg_035

Organism

Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]

Taxonomic identifier

198804 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	1342 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP MF_01321
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_01321
Subunit structure	The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.
Sequence similarities	Belongs to the RNA polymerase beta chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1342	1342	DNA-directed RNA polymerase subunit beta HAMAP MF_01321		PRO_0000047873

Sequences

	Sequence		Length	Mass (Da)
	P41184 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 7E30BC9E013D31ED	FASTA	1,342	152,061
	10 20 30 40 50 60 MVYSYTEKKR IRKDFGKRPQ VLDIPYLLSI QLDSFKKFIK IDPEGLHGLE AAFRSVFPIC 70 80 90 100 110 120 GYNGNSELQY VSYRLGDAIF DVKECQIRGA TYSAPLRVRL RLVIYERDVL EPTVKDIKEQ 130 140 150 160 170 180 EVYMGEIPLM TNNGTFIING TERVVVSQLH RSPGVFFDSD KGKTHSSGKV LYNARIIPYR 190 200 210 220 230 240 GSWLDFEFDP KDNLFVRIDR RRKLPVTIIL RALNYNTEEI LNLFFEKNIF NINNNKIQLE 250 260 270 280 290 300 LVSERLRGET ASFDIKKNGK IYVKKGRRIT AKHIQELKKD KINSITVPVE YILGRIVSKN 310 320 330 340 350 360 YLDPKTGETI ILANTELSLE ILTKLKNSSF FSIETLFTND LDHGPYISET LRIDSSHDRI 370 380 390 400 410 420 SALMEIYRVM RPGEPLTKEA TENLFENLFF SEDRYDLSSV GRMKFNRSLL RKKIEGVSTL 430 440 450 460 470 480 NKEDIIDVIK KLIDIRNGKG EVDDIDHLGN RRIRSVGEMA ENQFRIGLVR VERAVKERLS 490 500 510 520 530 540 VGDLDTLMPQ DMINAKPISA AIKEFFGSSQ LSQFMDQNNP LSEITHKRRI SALGLGGLTR 550 560 570 580 590 600 ERAGFEVRDV HPTHYGRVCP IETPEGPNIG LINSLSVYAR TNSYGFLETP YRKVHNRLVT 610 620 630 640 650 660 DEIHYLSAIE EGNYVIAQAN TNIDKNNYFI DDLVTCRHKG ESSLFNCNQV DYMDVSTQQI 670 680 690 700 710 720 VSVGASLIPF LEHDDANRAL MGANMQRQAV PTLKTDKPLV GTGMERAVAV DSGVTVVAKR 730 740 750 760 770 780 GGIIQYIDAS RIIIKVNEEE TYTGEAGIDI YNLTKYTRSN QNTCINQKPC VKLREKINKN 790 800 810 820 830 840 DVLADGPSTD LGELALGQNM RVAFMPWNGY NFEDSILVSE KVVQEDRFTT IHIQELSCIS 850 860 870 880 890 900 RDTKLGPEEI SSDIPNVGEA ALSKLDESGI VYIGAEVTGG DILVGKVTPK GETQLTPEEK 910 920 930 940 950 960 LLRAIFGEKA SDVKDSSLRV PNGVSGTVID VQIFTRDGVK KDKRALEIEN MQLKKAKKDL 970 980 990 1000 1010 1020 TEEFKIFELS LFSRIKKTLV SFNIKEDFLN KLPYEKWFKI DIQDRNKKKE IEKLLQQHNQ 1030 1040 1050 1060 1070 1080 LKKEFEKKIE VKRRKITQGD DLAPGVLKIV KVYLAVKRQI QPGDKMAGRH GNKGVISKIN 1090 1100 1110 1120 1130 1140 PVEDMPYDEN GIPVDIVLNP LGVPSRMNIG QILETHLGMA AKGIGNKINN MLKKQEKISN 1150 1160 1170 1180 1190 1200 LKKFIQKAFD LGENLRQKVN LDDFSNEEIL DLAKNLKKGM PISTPVFDGA QENEIKQMLK 1210 1220 1230 1240 1250 1260 FSNLPTSGQI SLFDGRTGEK FERPVTVGYM YMLKLNHLVD DKMHARSTGS YSLVTQQPLG 1270 1280 1290 1300 1310 1320 GKAQFGGQRF GEMEVWALEA YGASYTLQEM LTVKSDDVNG RTKMYKNIVD GNHQMEPGMP 1330 1340 ESFNVLLKEI RSLGINIELE NE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of an aphid endosymbiont DNA fragment containing rpoB (beta-subunit of RNA polymerase) and portions of rplL and rpoC." Clark M.A., Baumann L., Baumann P. Curr. Microbiol. 25:283-290(1992) [PubMed: 1369199] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed: 12089438] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sg.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z11913 Genomic DNA. Translation: CAA77970.1. AE013218 Genomic DNA. Translation: AAM67606.1.
PIR	S32680.
RefSeq	NP_660395.1. NC_004061.1.
3D structure databases
ProteinModelPortal	P41184.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000000230; EBBUCP00000000230; EBBUCG00000000230.
GeneID	1005851.
GenomeReviews	Gene locus BUsg_035 in contig AE013218_GR.
KEGG	bas:BUsg035.
PATRIC	21246827. VBIBucAph100086_0035.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000007841.
HOGENOM	HBG285547.
OMA	VDARDTK.
ProtClustDB	PRK00405.
Enzyme and pathway databases
BioCyc	BAPH198804:BUSG035-MONOMER.
Family and domain databases
HAMAP	MF_01321. RNApol_bact_RpoB. [Tree]
InterPro	IPR010243. DNA-dir_RNA_pol_bsu_bac-type. IPR019462. DNA-dir_RNA_pol_bsu_external_1. IPR015712. DNA-dir_RNA_pol_su2. IPR007120. DNA-dir_RNA_pol_su2_6. IPR007121. RNA_pol_bsu_CS. IPR007644. RNA_pol_bsu_protrusion. IPR007642. RNA_pol_Rpb2_2. IPR007645. RNA_pol_Rpb2_3. IPR007641. RNA_pol_Rpb2_7. IPR014724. RNA_pol_RPB2_OB-fold. [Graphical view]
Gene3D	G3DSA:2.30.150.10. G3DSA:2.30.150.10. 1 hit. G3DSA:2.40.270.10. G3DSA:2.40.270.10. 2 hits. G3DSA:2.40.50.150. Ribosomal_L2. 2 hits.
KO	K03043.
PANTHER	PTHR20856:SF3. PTHR20856:SF3. 1 hit. PTHR20856. RNA_pol_I_sub2. 1 hit.
Pfam	PF04563. RNA_pol_Rpb2_1. 1 hit. PF04561. RNA_pol_Rpb2_2. 2 hits. PF04565. RNA_pol_Rpb2_3. 1 hit. PF10385. RNA_pol_Rpb2_45. 1 hit. PF00562. RNA_pol_Rpb2_6. 1 hit. PF04560. RNA_pol_Rpb2_7. 1 hit. [Graphical view]
TIGRFAMs	TIGR02013. RpoB. 1 hit.
PROSITE	PS01166. RNA_POL_BETA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPOB_BUCAP

Accession

Primary (citable) accession number: P41184

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	January 25, 2012
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program