P41183 (BCL6_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
B-cell lymphoma 6 protein homolog

Gene names

Name:	Bcl6
Synonyms:	Bcl-6

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	707 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Transcriptional repressor which is required for germinal center formation and antibody affinity maturation. Probably plays an important role in lymphomagenesis By similarity.
Subunit structure	Homodimer. Interacts (via BTB domain) with BCOR; the interaction is direct. Interacts (via BTB domain) with NCOR2; the interaction is direct. Interacts with ZBTB7 and BCL6B. Interacts with the catalytic domain of HDAC9 By similarity. Ref.4 Ref.5
Subcellular location	Nucleus Ref.5.
Domain	The BTB domain mediates homodimerization. Its dimer interface mediates peptide binding such as to corepressors BCOR and NCOR2 By similarity.
Post-translational modification	Phosphorylated by MAPK1 in response to antigen receptor activation at Ser-334 and Ser-344. Phosphorylation induces its degradation by ubiquitin/proteasome pathway By similarity. Polyubiquitinated. Polyubiquitination leads to degradation by the proteasome By similarity.
Sequence similarities	Contains 1 BTB (POZ) domain. Contains 6 C2H2-type zinc fingers.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Activator Repressor
PTM	Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	B cell differentiation Inferred from genetic interaction PubMed 14647274. Source: MGI Rho protein signal transduction Inferred from genetic interaction PubMed 15860730. Source: MGI actin cytoskeleton organization Inferred from mutant phenotype PubMed 15860730. Source: MGI cell morphogenesis Inferred from mutant phenotype PubMed 15860730. Source: MGI erythrocyte development Inferred from mutant phenotype PubMed 15661395. Source: MGI germinal center formation Inferred from mutant phenotype PubMed 12354385PubMed 9110977PubMed 9171827PubMed 9236196. Source: MGI negative regulation of Rho protein signal transduction Inferred from mutant phenotype PubMed 15860730. Source: MGI negative regulation of T-helper 2 cell differentiation Inferred from genetic interaction PubMed 12594267. Source: MGI negative regulation of apoptotic process Inferred from direct assay PubMed 9927203. Source: MGI negative regulation of cell growth Inferred from electronic annotation. Source: Compara negative regulation of cell proliferation Inferred from mutant phenotype PubMed 15507530PubMed 15611242. Source: MGI negative regulation of cell-matrix adhesion Inferred from mutant phenotype PubMed 15860730. Source: MGI negative regulation of isotype switching to IgE isotypes Inferred from mutant phenotype PubMed 10490661. Source: MGI negative regulation of mast cell cytokine production Inferred from mutant phenotype PubMed 15950739. Source: MGI negative regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 10490661PubMed 12097386PubMed 12817026PubMed 15240705PubMed 15507530PubMed 15659391PubMed 17125145Ref.4. Source: MGI negative regulation of type 2 immune response Inferred from genetic interaction PubMed 10438949. Source: MGI positive regulation of B cell proliferation Inferred from mutant phenotype PubMed 9171827. Source: MGI positive regulation of apoptotic process Inferred from electronic annotation. Source: Compara positive regulation of cellular component movement Inferred from mutant phenotype PubMed 15860730. Source: MGI protein import into nucleus, translocation Inferred from electronic annotation. Source: Compara protein localization Inferred from mutant phenotype PubMed 15860730. Source: MGI regulation of Rho GTPase activity Inferred from mutant phenotype PubMed 15860730. Source: MGI regulation of inflammatory response Inferred from mutant phenotype PubMed 9110977. Source: MGI regulation of memory T cell differentiation Inferred from mutant phenotype PubMed 15240675. Source: MGI response to DNA damage stimulus Inferred from electronic annotation. Source: Compara spermatogenesis Inferred from mutant phenotype PubMed 11092811. Source: MGI transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW type 2 immune response Inferred from mutant phenotype PubMed 9171827. Source: MGI
Cellular_component	nucleus Inferred from direct assay Ref.4. Source: MGI replication fork Inferred from electronic annotation. Source: Compara
Molecular_function	chromatin DNA binding Inferred from direct assay PubMed 17125145. Source: MGI sequence-specific DNA binding Inferred from direct assay PubMed 15659391. Source: MGI sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: Compara zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Tbx21	Q9JKD8	3	EBI-6253762,EBI-3863870

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 707	707	B-cell lymphoma 6 protein homolog		PRO_0000047099
Regions
	Domain	32 – 99	68	BTB
	Zinc finger	519 – 542	24	C2H2-type 1
	Zinc finger	547 – 569	23	C2H2-type 2
	Zinc finger	575 – 597	23	C2H2-type 3
	Zinc finger	603 – 625	23	C2H2-type 4
	Zinc finger	631 – 653	23	C2H2-type 5
	Zinc finger	659 – 682	24	C2H2-type 6
Amino acid modifications
	Modified residue	334	1	Phosphoserine; by MAPK1 By similarity
	Modified residue	344	1	Phosphoserine; by MAPK1 By similarity
Experimental info
	Sequence conflict	456	1	A → G in AAB17432. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P41183 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 2051DD808D32D5EC	FASTA	707	78,982
	10 20 30 40 50 60 MASPADSCIQ FTRHASDVLL NLNRLRSRDI LTDVVIVVSR EQFRAHKTVL MACSGLFYSI 70 80 90 100 110 120 FTDQLKCNLS VINLDPEISP EGFCILLDFM YTSRLNLREG NIMAVMTTAM YLQMEHVVDT 130 140 150 160 170 180 CRKFIKASEA EMAPALKPPR EEFLNSRMLM PHDIMAYRGR EVVENNMPLR NTPGCESRAF 190 200 210 220 230 240 APPLYSGLST PPASYPMYSH LPLSTFLFSD EELRDAPRMP VANPFPKERA LPCDSARQVP 250 260 270 280 290 300 NEYSRPAMEV SPSLCHSNIY SPKEAVPEEA RSDIHYSVPE GPKPAVPSAR NAPYFPCDKA 310 320 330 340 350 360 SKEEERPSSE DEIALHFEPP NAPLNRKGLV SPQSPQKSDC QPNSPTESCS SKNACILQAS 370 380 390 400 410 420 GSPPAKSPTD PKACNWKKYK FIVLNSLNQN AKPEGSEQAE LGRLSPRAYP APPACQPPME 430 440 450 460 470 480 PANLDLQSPT KLSASGEDST IPQASRLNNL VNRSLAGSPR SSSESHSPLY MHPPKCTSCG 490 500 510 520 530 540 SQSPQHTEMC LHTAGPTFPE EMGETQSEYS DSSCENGTFF CNECDCRFSE EASLKRHTLQ 550 560 570 580 590 600 THSDKPYKCD RCQASFRYKG NLASHKTVHT GEKPYRCNIC GAQFNRPANL KTHTRIHSGE 610 620 630 640 650 660 KPYKCETCGA RFVQVAHLRA HVLIHTGEKP YPCEICGTRF RHLQTLKSHL RIHTGEKPYH 670 680 690 700 CEKCNLHFRH KSQLRLHLRQ KHGAITNTKV QYRVSAADLP PELPKAC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The murine BCL6 gene is induced in activated lymphocytes as an immediate early gene." Fukuda T., Miki T., Yoshida T., Hatano M., Ohashi K., Hirosawa S., Tokuhisa T. Oncogene 11:1657-1663(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Skeletal muscle.
[2]	"BCL-6 expression during B-cell activation." Allman D., Jain A., Dent A., Maile R.R., Selvaggi T., Kehry M.R., Staudt L.M. Blood 87:5257-5268(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Muscle.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Olfactory epithelium.
[4]	"BAZF, a novel Bcl6 homolog, functions as a transcriptional repressor." Okabe S., Fukuda T., Ishibashi K., Kojima S., Okada S., Hatano M., Ebara M., Saisho H., Tokuhisa T. Mol. Cell. Biol. 18:4235-4244(1998) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH BCL6B.
[5]	"Novel BTB/POZ domain zinc-finger protein, LRF, is a potential target of the LAZ-3/BCL-6 oncogene." Davies J.M., Hawe N., Kabarowski J., Huang Q.-H., Zhu J., Brand N.J., Leprince D., Dhordain P., Cook M., Moriss-Kay G., Zelent A. Oncogene 18:365-375(1999) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ZBTB7, SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D38377 mRNA. Translation: BAA07456.1. U41465 mRNA. Translation: AAB17432.1. BC052315 mRNA. Translation: AAH52315.1.
IPI	IPI00111606.
RefSeq	NP_033874.1. NM_009744.3.
UniGene	Mm.347398.
3D structure databases
ProteinModelPortal	P41183.
SMR	P41183. Positions 5-129, 473-679.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-59432N.
IntAct	P41183. 1 interaction.
PTM databases
PhosphoSite	P41183.
Proteomic databases
PRIDE	P41183.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000023151; ENSMUSP00000023151; ENSMUSG00000022508.
GeneID	12053.
KEGG	mmu:12053.
UCSC	uc007ytz.1. mouse.
Organism-specific databases
CTD	604.
MGI	MGI:107187. Bcl6.
Phylogenomic databases
eggNOG	COG5048.
HOGENOM	HOG000001556.
HOVERGEN	HBG004831.
InParanoid	P41183.
KO	K15618.
OMA	NECDCRF.
OrthoDB	EOG4NVZJS.
Gene expression databases
ArrayExpress	P41183.
Bgee	P41183.
CleanEx	MM_BCL6.
Genevestigator	P41183.
GermOnline	ENSMUSG00000022508. Mus musculus.
Family and domain databases
Gene3D	3.30.160.60. 5 hits. 3.30.710.10. 1 hit.
InterPro	IPR000210. BTB/POZ-like. IPR011333. BTB/POZ_fold. IPR013069. BTB_POZ. IPR007087. Znf_C2H2. IPR015880. Znf_C2H2-like. IPR013087. Znf_C2H2/integrase_DNA-bd. [Graphical view]
Pfam	PF00651. BTB. 1 hit. [Graphical view]
SMART	SM00225. BTB. 1 hit. SM00355. ZnF_C2H2. 6 hits. [Graphical view]
SUPFAM	SSF54695. BTB/POZ_fold. 1 hit.
PROSITE	PS50097. BTB. 1 hit. PS00028. ZINC_FINGER_C2H2_1. 6 hits. PS50157. ZINC_FINGER_C2H2_2. 6 hits. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	BCL6. mouse.
NextBio	280351.
SOURCE	Search...

Entry information

Entry name

BCL6_MOUSE

Accession

Primary (citable) accession number: P41183
Secondary accession number(s): Q61065

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 29, 2013
This is version 115 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program