P41160 (LEP_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Leptin

Alternative name(s):
Obesity factor

Gene names

Name:	Lep
Synonyms:	Ob

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	167 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
Subcellular location	Secreted Probable.
Involvement in disease	Defects in Lep are the cause of profound obesity and type II diabetes.
Sequence similarities	Belongs to the leptin family.

Ontologies

Keywords
Cellular component	Secreted
Disease	Diabetes mellitus Obesity
Domain	Signal
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	adipose tissue development Inferred from genetic interaction PubMed 10742101. Source: MGI adult feeding behavior Inferred from direct assay PubMed 9590691. Source: HGNC bile acid metabolic process Inferred from direct assay PubMed 12114517. Source: MGI bone mineralization involved in bone maturation Inferred from electronic annotation. Source: Compara cellular response to L-ascorbic acid Inferred from electronic annotation. Source: Compara cellular response to retinoic acid Inferred from electronic annotation. Source: Compara central nervous system neuron development Inferred from mutant phenotype PubMed 10592285. Source: MGI cholesterol metabolic process Inferred from genetic interaction PubMed 15995171. Source: MGI circadian rhythm Inferred from electronic annotation. Source: Compara eating behavior Inferred from genetic interaction PubMed 10802666. Source: MGI energy reserve metabolic process Inferred from electronic annotation. Source: Compara fatty acid catabolic process Inferred from electronic annotation. Source: Compara female pregnancy Inferred from electronic annotation. Source: Compara glucose metabolic process Inferred from mutant phenotype PubMed 12540600. Source: MGI glycerol biosynthetic process Inferred from electronic annotation. Source: Compara hormone metabolic process Inferred from mutant phenotype PubMed 16513828. Source: MGI insulin secretion Inferred from genetic interaction PubMed 16141392. Source: MGI leptin-mediated signaling pathway Inferred from electronic annotation. Source: Compara leukocyte tethering or rolling Inferred from electronic annotation. Source: Compara negative regulation of apoptotic process Inferred from electronic annotation. Source: Compara negative regulation of appetite Inferred from direct assay PubMed 9590691. Source: HGNC negative regulation of cartilage development Inferred from electronic annotation. Source: Compara negative regulation of glucagon secretion Inferred from direct assay PubMed 21234744. Source: BHF-UCL negative regulation of glutamine transport Inferred from electronic annotation. Source: Compara negative regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 21234744. Source: BHF-UCL negative regulation of vasoconstriction Inferred from electronic annotation. Source: Compara ovulation from ovarian follicle Inferred from electronic annotation. Source: Compara placenta development Inferred from electronic annotation. Source: Compara positive regulation of MAPK cascade Inferred from electronic annotation. Source: Compara positive regulation of STAT protein import into nucleus Inferred from direct assay PubMed 21234744. Source: BHF-UCL positive regulation of cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of cytokine production Inferred from electronic annotation. Source: Compara positive regulation of developmental growth Inferred from electronic annotation. Source: Compara positive regulation of follicle-stimulating hormone secretion Inferred from electronic annotation. Source: Compara positive regulation of hepatic stellate cell activation Inferred from electronic annotation. Source: Compara positive regulation of insulin receptor signaling pathway Inferred from electronic annotation. Source: Compara positive regulation of ion transport Inferred from electronic annotation. Source: Compara positive regulation of luteinizing hormone secretion Inferred from electronic annotation. Source: Compara positive regulation of myeloid cell differentiation Inferred from direct assay PubMed 9057630. Source: MGI positive regulation of tyrosine phosphorylation of Stat3 protein Inferred from electronic annotation. Source: Compara regulation of blood pressure Inferred from electronic annotation. Source: Compara regulation of gluconeogenesis Inferred from mutant phenotype PubMed 16513828. Source: MGI regulation of insulin secretion Inferred from mutant phenotype PubMed 16513828. Source: MGI regulation of intestinal cholesterol absorption Inferred from direct assay PubMed 12114517. Source: MGI regulation of lipoprotein lipid oxidation Inferred from electronic annotation. Source: Compara regulation of protein phosphorylation Inferred from direct assay PubMed 21234744. Source: BHF-UCL regulation of steroid biosynthetic process Inferred from mutant phenotype PubMed 16513828. Source: MGI response to dietary excess Inferred from mutant phenotype PubMed 16141392. Source: MGI response to hypoxia Inferred from electronic annotation. Source: Compara response to insulin stimulus Inferred from mutant phenotype PubMed 16141392. Source: MGI response to vitamin E Inferred from electronic annotation. Source: Compara tyrosine phosphorylation of STAT protein Inferred from direct assay PubMed 21234744. Source: BHF-UCL
Cellular_component	cytoplasm Inferred from direct assay PubMed 11387233. Source: BHF-UCL extracellular space Inferred from direct assay PubMed 11387233. Source: BHF-UCL
Molecular_function	growth factor activity Inferred from direct assay PubMed 9057630. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 167	146	Leptin		PRO_0000017687
Amino acid modifications
	Disulfide bond	117 ↔ 167		By similarity
Natural variations
	Natural variant	49	1	Missing in 30% the clones.

Sequences

	Sequence		Length	Mass (Da)
	P41160 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: D6783E6C76FD7116	FASTA	167	18,709
	10 20 30 40 50 60 MCWRPLCRFL WLWSYLSYVQ AVPIQKVQDD TKTLIKTIVT RINDISHTQS VSAKQRVTGL 70 80 90 100 110 120 DFIPGLHPIL SLSKMDQTLA VYQQVLTSLP SQNVLQIAND LENLRDLLHL LAFSKSCSLP 130 140 150 160 QTSGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQ LDVSPEC

References

[1]	"Positional cloning of the mouse obese gene and its human homologue." Zhang Y., Proenca P., Maffei M., Barone M., Leopold L., Friedman J.M. Nature 372:425-432(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Erratum Zhang Y., Proenca P., Maffei M., Barone M., Leopold L., Friedman J.M. Nature 374:479-479(1995)
[3]	Chehab F.F., Lim M.E. Submitted (MAR-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C57BL/6J.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18812 mRNA. Translation: AAA64564.1. U22421 Genomic DNA. Translation: AAA64213.1.
IPI	IPI00111526.
PIR	LTMS. S50863.
RefSeq	NP_032519.1. NM_008493.3.
UniGene	Mm.277072.
3D structure databases
ProteinModelPortal	P41160.
SMR	P41160. Positions 24-167.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000067046.
PTM databases
PhosphoSite	P41160.
Proteomic databases
PRIDE	P41160.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000069789; ENSMUSP00000067046; ENSMUSG00000059201.
GeneID	16846.
KEGG	mmu:16846.
Organism-specific databases
CTD	3952.
MGI	MGI:104663. Lep.
Phylogenomic databases
eggNOG	NOG45133.
HOGENOM	HOG000252923.
HOVERGEN	HBG007860.
InParanoid	P41160.
KO	K05424.
OMA	KTIVTRI.
OrthoDB	EOG4QNMXC.
Gene expression databases
ArrayExpress	P41160.
Bgee	P41160.
CleanEx	MM_LEP.
Genevestigator	P41160.
GermOnline	ENSMUSG00000059201. Mus musculus.
Family and domain databases
Gene3D	1.20.1250.10. 1 hit.
InterPro	IPR009079. 4_helix_cytokine-like_core. IPR012351. 4_helix_cytokine_core. IPR000065. Leptin. [Graphical view]
PANTHER	PTHR11724. PTHR11724. 1 hit.
Pfam	PF02024. Leptin. 1 hit. [Graphical view]
PIRSF	PIRSF001837. Leptin. 1 hit.
PRINTS	PR00495. LEPTIN.
ProDom	PD005698. Leptin. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF47266. 4_helix_cytokine. 1 hit.
ProtoNet	Search...
Other
NextBio	290772.
SOURCE	Search...

Entry information

Entry name

LEP_MOUSE

Accession

Primary (citable) accession number: P41160

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	April 3, 2013
This is version 112 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program