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Protein

Heat stress transcription factor A-1a

Gene

HSFA1A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional activator that specifically binds DNA sequence 5'-AGAAnnTTCT-3' known as heat shock promoter elements (HSE).

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi50 – 144By similarityAdd BLAST95

GO - Molecular functioni

  • DNA binding Source: TAIR
  • DNA binding transcription factor activity Source: TAIR
  • sequence-specific DNA binding Source: InterPro

GO - Biological processi

  • response to heat Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, DNA-binding
Biological processStress response, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-ATH-3371453 Regulation of HSF1-mediated heat shock response
R-ATH-3371511 HSF1 activation
R-ATH-3371568 Attenuation phase
R-ATH-3371571 HSF1-dependent transactivation

Names & Taxonomyi

Protein namesi
Recommended name:
Heat stress transcription factor A-1a
Short name:
AtHsfA1a
Alternative name(s):
AtHsf-13
Heat shock factor protein 1
Short name:
HSF 1
Heat shock transcription factor 1
Short name:
HSTF 1
Gene namesi
Name:HSFA1A
Synonyms:HSF1, HSF13
Ordered Locus Names:At4g17750
ORF Names:dl4910c, FCAALL.107
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G17750
TAIRilocus:2005495 AT4G17750

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001245821 – 495Heat stress transcription factor A-1aAdd BLAST495

Post-translational modificationi

Exhibits temperature-dependent phosphorylation (By similarity). Phosphorylated by CRK1.By similarity1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP41151

PTM databases

iPTMnetiP41151

Expressioni

Tissue specificityi

Constitutively expressed.1 Publication

Inductioni

By heat stress.1 Publication

Gene expression databases

ExpressionAtlasiP41151 baseline and differential
GenevisibleiP41151 AT

Interactioni

Subunit structurei

Homotrimer. Interacts with HSP70-1 and HSP70-4. Binds to CRK1.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi12789, 17 interactors
IntActiP41151, 6 interactors
STRINGi3702.AT4G17750.1

Structurei

3D structure databases

ProteinModelPortaliP41151
SMRiP41151
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni172 – 238Hydrophobic repeat HR-A/BAdd BLAST67

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi262 – 268Nuclear localization signalSequence analysis7
Motifi433 – 442AHA10
Motifi482 – 489Nuclear export signalSequence analysis8

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi150 – 153Poly-Ser4
Compositional biasi204 – 207Poly-Gln4
Compositional biasi454 – 475Poly-AsnAdd BLAST22

Domaini

The hydrophobic-rich region (HR-A/B) corresponds to the oligomerization domain. AHA motif is a transcriptional activator element.1 Publication

Sequence similaritiesi

Belongs to the HSF family. Class A subfamily.Curated

Phylogenomic databases

eggNOGiKOG0627 Eukaryota
COG5169 LUCA
HOGENOMiHOG000237979
InParanoidiP41151
KOiK09419
OMAiRESINDF
OrthoDBiEOG09360BUI
PhylomeDBiP41151

Family and domain databases

Gene3Di1.10.10.10, 1 hit
InterProiView protein in InterPro
IPR000232 HSF_DNA-bd
IPR027725 HSF_fam
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR10015 PTHR10015, 1 hit
PfamiView protein in Pfam
PF00447 HSF_DNA-bind, 1 hit
PRINTSiPR00056 HSFDOMAIN
SMARTiView protein in SMART
SM00415 HSF, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
PROSITEiView protein in PROSITE
PS00434 HSF_DOMAIN, 1 hit

Sequencei

Sequence statusi: Complete.

P41151-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFVNFKYFSF FIRTKMDGVT GGGTNIGEAV TAPPPRNPHP ATLLNANSLP
60 70 80 90 100
PPFLSKTYDM VEDPATDAIV SWSPTNNSFI VWDPPEFSRD LLPKYFKHNN
110 120 130 140 150
FSSFVRQLNT YGFRKVDPDR WEFANEGFLR GQKHLLKKIS RRKSVQGHGS
160 170 180 190 200
SSSNPQSQQL SQGQGSMAAL SSCVEVGKFG LEEEVEQLKR DKNVLMQELV
210 220 230 240 250
KLRQQQQTTD NKLQVLVKHL QVMEQRQQQI MSFLAKAVQN PTFLSQFIQK
260 270 280 290 300
QTDSNMHVTE ANKKRRLRED STAATESNSH SHSLEASDGQ IVKYQPLRND
310 320 330 340 350
SMMWNMMKTD DKYPFLDGFS SPNQVSGVTL QEVLPITSGQ SQAYASVPSG
360 370 380 390 400
QPLSYLPSTS TSLPDTIMPE TSQIPQLTRE SINDFPTENF MDTEKNVPEA
410 420 430 440 450
FISPSPFLDG GSVPIQLEGI PEDPEIDELM SNFEFLEEYM PESPVFGDAT
460 470 480 490
TLENNNNNNN NNNNNNNNNN NNNTNGRHMD KLIEELGLLT SETEH
Length:495
Mass (Da):55,744
Last modified:December 1, 2000 - v2
Checksum:i2F0DE55252B5682A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti216L → M in CAA53761 (PubMed:7948881).Curated1
Sequence conflicti285E → D in CAA53761 (PubMed:7948881).Curated1
Sequence conflicti336I → T in CAA53761 (PubMed:7948881).Curated1
Sequence conflicti390F → Y in CAA53761 (PubMed:7948881).Curated1
Sequence conflicti462 – 473Missing in CAA53761 (PubMed:7948881).CuratedAdd BLAST12

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76167 Genomic DNA Translation: CAA53761.1
Z97344 Genomic DNA Translation: CAB10555.1
AL161547 Genomic DNA Translation: CAB78778.1
CP002687 Genomic DNA Translation: AEE83945.1
AK229347 mRNA Translation: BAF01210.1
PIRiF71447 S52641
RefSeqiNP_193510.1, NM_117884.3
UniGeneiAt.32971

Genome annotation databases

EnsemblPlantsiAT4G17750.1; AT4G17750.1; AT4G17750
GeneIDi827496
GrameneiAT4G17750.1; AT4G17750.1; AT4G17750
KEGGiath:AT4G17750

Similar proteinsi

Entry informationi

Entry nameiHFA1A_ARATH
AccessioniPrimary (citable) accession number: P41151
Secondary accession number(s): O23615, Q0WNT9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: December 1, 2000
Last modified: April 25, 2018
This is version 152 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health