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Protein

Heat stress transcription factor A-1a

Gene

HSFA1A

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional activator that specifically binds DNA sequence 5'-AGAAnnTTCT-3' known as heat shock promoter elements (HSE).

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi50 – 14495By similarityAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: TAIR
  • sequence-specific DNA binding Source: InterPro
  • transcription factor activity, sequence-specific DNA binding Source: TAIR

GO - Biological processi

  • response to heat Source: TAIR
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Stress response, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-ATH-3371453. Regulation of HSF1-mediated heat shock response.
R-ATH-3371511. HSF1 activation.
R-ATH-3371568. Attenuation phase.
R-ATH-3371571. HSF1-dependent transactivation.

Names & Taxonomyi

Protein namesi
Recommended name:
Heat stress transcription factor A-1a
Short name:
AtHsfA1a
Alternative name(s):
AtHsf-13
Heat shock factor protein 1
Short name:
HSF 1
Heat shock transcription factor 1
Short name:
HSTF 1
Gene namesi
Name:HSFA1A
Synonyms:HSF1, HSF13
Ordered Locus Names:At4g17750
ORF Names:dl4910c, FCAALL.107
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

TAIRiAT4G17750.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 495495Heat stress transcription factor A-1aPRO_0000124582Add
BLAST

Post-translational modificationi

Exhibits temperature-dependent phosphorylation (By similarity). Phosphorylated by CRK1.By similarity1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP41151.
PRIDEiP41151.

PTM databases

iPTMnetiP41151.

Expressioni

Tissue specificityi

Constitutively expressed.1 Publication

Inductioni

By heat stress.1 Publication

Gene expression databases

GenevisibleiP41151. AT.

Interactioni

Subunit structurei

Homotrimer. Interacts with HSP70-1 and HSP70-4. Binds to CRK1.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CRK1O806733EBI-1544927,EBI-1804894
TBP1P281473EBI-1544927,EBI-1247453
TBP2P281482EBI-1544927,EBI-1544976

Protein-protein interaction databases

BioGridi12789. 17 interactions.
IntActiP41151. 6 interactions.
MINTiMINT-8061297.
STRINGi3702.AT4G17750.1.

Structurei

3D structure databases

ProteinModelPortaliP41151.
SMRiP41151. Positions 51-142.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni172 – 23867Hydrophobic repeat HR-A/BAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi262 – 2687Nuclear localization signalSequence analysis
Motifi433 – 44210AHA
Motifi482 – 4898Nuclear export signalSequence analysis

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi150 – 1534Poly-Ser
Compositional biasi204 – 2074Poly-Gln
Compositional biasi454 – 47522Poly-AsnAdd
BLAST

Domaini

The hydrophobic-rich region (HR-A/B) corresponds to the oligomerization domain. AHA motif is a transcriptional activator element.1 Publication

Sequence similaritiesi

Belongs to the HSF family. Class A subfamily.Curated

Phylogenomic databases

eggNOGiKOG0627. Eukaryota.
COG5169. LUCA.
HOGENOMiHOG000237979.
InParanoidiP41151.
KOiK09419.
OMAiQIMSFLA.
OrthoDBiEOG09360BUI.
PhylomeDBiP41151.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR000232. HSF_DNA-bd.
IPR027725. HSF_fam.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10015. PTHR10015. 1 hit.
PfamiPF00447. HSF_DNA-bind. 1 hit.
[Graphical view]
PRINTSiPR00056. HSFDOMAIN.
SMARTiSM00415. HSF. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS00434. HSF_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P41151-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFVNFKYFSF FIRTKMDGVT GGGTNIGEAV TAPPPRNPHP ATLLNANSLP
60 70 80 90 100
PPFLSKTYDM VEDPATDAIV SWSPTNNSFI VWDPPEFSRD LLPKYFKHNN
110 120 130 140 150
FSSFVRQLNT YGFRKVDPDR WEFANEGFLR GQKHLLKKIS RRKSVQGHGS
160 170 180 190 200
SSSNPQSQQL SQGQGSMAAL SSCVEVGKFG LEEEVEQLKR DKNVLMQELV
210 220 230 240 250
KLRQQQQTTD NKLQVLVKHL QVMEQRQQQI MSFLAKAVQN PTFLSQFIQK
260 270 280 290 300
QTDSNMHVTE ANKKRRLRED STAATESNSH SHSLEASDGQ IVKYQPLRND
310 320 330 340 350
SMMWNMMKTD DKYPFLDGFS SPNQVSGVTL QEVLPITSGQ SQAYASVPSG
360 370 380 390 400
QPLSYLPSTS TSLPDTIMPE TSQIPQLTRE SINDFPTENF MDTEKNVPEA
410 420 430 440 450
FISPSPFLDG GSVPIQLEGI PEDPEIDELM SNFEFLEEYM PESPVFGDAT
460 470 480 490
TLENNNNNNN NNNNNNNNNN NNNTNGRHMD KLIEELGLLT SETEH
Length:495
Mass (Da):55,744
Last modified:December 1, 2000 - v2
Checksum:i2F0DE55252B5682A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti216 – 2161L → M in CAA53761 (PubMed:7948881).Curated
Sequence conflicti285 – 2851E → D in CAA53761 (PubMed:7948881).Curated
Sequence conflicti336 – 3361I → T in CAA53761 (PubMed:7948881).Curated
Sequence conflicti390 – 3901F → Y in CAA53761 (PubMed:7948881).Curated
Sequence conflicti462 – 47312Missing in CAA53761 (PubMed:7948881).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76167 Genomic DNA. Translation: CAA53761.1.
Z97344 Genomic DNA. Translation: CAB10555.1.
AL161547 Genomic DNA. Translation: CAB78778.1.
CP002687 Genomic DNA. Translation: AEE83945.1.
AK229347 mRNA. Translation: BAF01210.1.
PIRiF71447. S52641.
RefSeqiNP_193510.1. NM_117884.2.
UniGeneiAt.32971.

Genome annotation databases

EnsemblPlantsiAT4G17750.1; AT4G17750.1; AT4G17750.
GeneIDi827496.
GrameneiAT4G17750.1; AT4G17750.1; AT4G17750.
KEGGiath:AT4G17750.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76167 Genomic DNA. Translation: CAA53761.1.
Z97344 Genomic DNA. Translation: CAB10555.1.
AL161547 Genomic DNA. Translation: CAB78778.1.
CP002687 Genomic DNA. Translation: AEE83945.1.
AK229347 mRNA. Translation: BAF01210.1.
PIRiF71447. S52641.
RefSeqiNP_193510.1. NM_117884.2.
UniGeneiAt.32971.

3D structure databases

ProteinModelPortaliP41151.
SMRiP41151. Positions 51-142.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi12789. 17 interactions.
IntActiP41151. 6 interactions.
MINTiMINT-8061297.
STRINGi3702.AT4G17750.1.

PTM databases

iPTMnetiP41151.

Proteomic databases

PaxDbiP41151.
PRIDEiP41151.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT4G17750.1; AT4G17750.1; AT4G17750.
GeneIDi827496.
GrameneiAT4G17750.1; AT4G17750.1; AT4G17750.
KEGGiath:AT4G17750.

Organism-specific databases

TAIRiAT4G17750.

Phylogenomic databases

eggNOGiKOG0627. Eukaryota.
COG5169. LUCA.
HOGENOMiHOG000237979.
InParanoidiP41151.
KOiK09419.
OMAiQIMSFLA.
OrthoDBiEOG09360BUI.
PhylomeDBiP41151.

Enzyme and pathway databases

ReactomeiR-ATH-3371453. Regulation of HSF1-mediated heat shock response.
R-ATH-3371511. HSF1 activation.
R-ATH-3371568. Attenuation phase.
R-ATH-3371571. HSF1-dependent transactivation.

Miscellaneous databases

PROiP41151.

Gene expression databases

GenevisibleiP41151. AT.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR000232. HSF_DNA-bd.
IPR027725. HSF_fam.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR10015. PTHR10015. 1 hit.
PfamiPF00447. HSF_DNA-bind. 1 hit.
[Graphical view]
PRINTSiPR00056. HSFDOMAIN.
SMARTiSM00415. HSF. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
PROSITEiPS00434. HSF_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHFA1A_ARATH
AccessioniPrimary (citable) accession number: P41151
Secondary accession number(s): O23615, Q0WNT9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: December 1, 2000
Last modified: September 7, 2016
This is version 141 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.