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Protein

Nociceptin receptor

Gene

OPRL1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

G-protein coupled opioid receptor that functions as receptor for the endogenous neuropeptide nociceptin. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling via G proteins mediates inhibition of adenylate cyclase activity and calcium channel activity. Arrestins modulate signaling via G proteins and mediate the activation of alternative signaling pathways that lead to the activation of MAP kinases. Plays a role in modulating nociception and the perception of pain. Plays a role in the regulation of locomotor activity by the neuropeptide nociceptin.5 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei110Important for G protein-mediated signaling1
Sitei130Important for G protein-mediated signaling1

GO - Molecular functioni

  • G-protein coupled receptor activity Source: UniProtKB
  • neuropeptide binding Source: GO_Central
  • nociceptin receptor activity Source: UniProtKB
  • protein C-terminus binding Source: Ensembl

GO - Biological processi

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer
Biological processBehavior

Enzyme and pathway databases

ReactomeiR-HSA-375276. Peptide ligand-binding receptors.
R-HSA-418594. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Nociceptin receptor
Alternative name(s):
Kappa-type 3 opioid receptor
Short name:
KOR-3
Orphanin FQ receptor
Gene namesi
Name:OPRL1
Synonyms:OOR, ORL1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 20

Organism-specific databases

EuPathDBiHostDB:ENSG00000125510.15.
HGNCiHGNC:8155. OPRL1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 48Extracellular1 PublicationAdd BLAST48
Transmembranei49 – 74Helical; Name=1Add BLAST26
Topological domaini75 – 87Cytoplasmic1 PublicationAdd BLAST13
Transmembranei88 – 109Helical; Name=2Add BLAST22
Topological domaini110 – 124Extracellular1 PublicationAdd BLAST15
Transmembranei125 – 146Helical; Name=3Add BLAST22
Topological domaini147 – 165Cytoplasmic1 PublicationAdd BLAST19
Transmembranei166 – 188Helical; Name=4Add BLAST23
Topological domaini189 – 211Extracellular1 PublicationAdd BLAST23
Transmembranei212 – 236Helical; Name=5Add BLAST25
Topological domaini237 – 264Cytoplasmic1 PublicationAdd BLAST28
Transmembranei265 – 285Helical; Name=6Add BLAST21
Topological domaini286 – 300Extracellular1 PublicationAdd BLAST15
Transmembranei301 – 322Helical; Name=7Add BLAST22
Topological domaini323 – 370Cytoplasmic1 PublicationAdd BLAST48

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi107Q → A: Impairs G protein-mediated inhibition of adenylate cyclase. 1 Publication1
Mutagenesisi110D → A: Abolishes G protein-mediated inhibition of adenylate cyclase. No effect on antagonist-mediated inhibition of G protein-mediated signaling. 1 Publication1
Mutagenesisi130D → A: Abolishes G protein-mediated inhibition of adenylate cyclase. Abolishes antagonist-mediated inhibition of G protein-mediated signaling. 1 Publication1
Mutagenesisi131Y → A: Impairs G protein-mediated inhibition of adenylate cyclase. 1 Publication1
Mutagenesisi280Q → A: Impairs G protein-mediated inhibition of adenylate cyclase. 1 Publication1
Mutagenesisi309Y → A: Mildly impairs G protein-mediated inhibition of adenylate cyclase. Abolishes antagonist-mediated inhibition of G protein-mediated signaling. 1 Publication1
Mutagenesisi337S → A: No effect on ligand-mediated internalization; when associated with A-346 and A-351. 1 Publication1
Mutagenesisi346S → A: No effect on ligand-mediated internalization; when associated with A-337 and A-351. 1 Publication1
Mutagenesisi351S → A: No effect on ligand-mediated internalization; when associated with A-337 and A-346. 1 Publication1
Mutagenesisi363S → A: Impairs ligand-mediated internalization. 1 Publication1

Organism-specific databases

DisGeNETi4987.
OpenTargetsiENSG00000125510.
PharmGKBiPA31944.

Chemistry databases

ChEMBLiCHEMBL2014.
DrugBankiDB05492. Epicept NP-1.
DB01497. Etorphine.
GuidetoPHARMACOLOGYi320.

Polymorphism and mutation databases

BioMutaiOPRL1.
DMDMi730230.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000699801 – 370Nociceptin receptorAdd BLAST370

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi21N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi28N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi39N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi123 ↔ 200PROSITE-ProRule annotation1 Publication
Lipidationi334S-palmitoyl cysteineSequence analysis1
Modified residuei363Phosphoserine1 Publication1

Post-translational modificationi

Phosphorylation at Ser-363 requires GRK3.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP41146.
PRIDEiP41146.

PTM databases

iPTMnetiP41146.
PhosphoSitePlusiP41146.

Expressioni

Tissue specificityi

Detected in blood leukocytes.1 Publication

Inductioni

By phytohemagglutinin (PHA).1 Publication

Gene expression databases

BgeeiENSG00000125510.
CleanExiHS_OPRL1.
GenevisibleiP41146. HS.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi111032. 3 interactors.
IntActiP41146. 2 interactors.
STRINGi9606.ENSP00000336764.

Chemistry databases

BindingDBiP41146.

Structurei

Secondary structure

1370
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi48 – 77Combined sources30
Helixi85 – 113Combined sources29
Helixi119 – 153Combined sources35
Helixi157 – 160Combined sources4
Helixi164 – 188Combined sources25
Beta strandi189 – 192Combined sources4
Beta strandi194 – 197Combined sources4
Beta strandi199 – 202Combined sources4
Helixi208 – 223Combined sources16
Helixi225 – 242Combined sources18
Helixi252 – 287Combined sources36
Helixi295 – 322Combined sources28
Helixi324 – 330Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4EA3X-ray3.01A/B43-339[»]
5DHGX-ray3.00A/B43-339[»]
5DHHX-ray3.00A/B43-339[»]
ProteinModelPortaliP41146.
SMRiP41146.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00760000118797.
HOGENOMiHOG000230486.
HOVERGENiHBG106919.
InParanoidiP41146.
KOiK04216.
OMAiRTPHKAK.
OrthoDBiEOG091G0HEN.
PhylomeDBiP41146.
TreeFamiTF315737.

Family and domain databases

CDDicd15092. 7tmA_NOFQ_opioid_R. 1 hit.
InterProiView protein in InterPro
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001418. Opioid_rcpt.
IPR001420. X_opioid_rcpt.
PfamiView protein in Pfam
PF00001. 7tm_1. 1 hit.
PRINTSiPR00237. GPCRRHODOPSN.
PR00384. OPIOIDR.
PR00547. XOPIOIDR.
SMARTiView protein in SMART
SM01381. 7TM_GPCR_Srsx. 1 hit.
PROSITEiView protein in PROSITE
PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P41146-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEPLFPAPFW EVIYGSHLQG NLSLLSPNHS LLPPHLLLNA SHGAFLPLGL
60 70 80 90 100
KVTIVGLYLA VCVGGLLGNC LVMYVILRHT KMKTATNIYI FNLALADTLV
110 120 130 140 150
LLTLPFQGTD ILLGFWPFGN ALCKTVIAID YYNMFTSTFT LTAMSVDRYV
160 170 180 190 200
AICHPIRALD VRTSSKAQAV NVAIWALASV VGVPVAIMGS AQVEDEEIEC
210 220 230 240 250
LVEIPTPQDY WGPVFAICIF LFSFIVPVLV ISVCYSLMIR RLRGVRLLSG
260 270 280 290 300
SREKDRNLRR ITRLVLVVVA VFVGCWTPVQ VFVLAQGLGV QPSSETAVAI
310 320 330 340 350
LRFCTALGYV NSCLNPILYA FLDENFKACF RKFCCASALR RDVQVSDRVR
360 370
SIAKDVALAC KTSETVPRPA
Length:370
Mass (Da):40,693
Last modified:February 1, 1995 - v1
Checksum:i613700C0B4D093BA
GO
Isoform 2 (identifier: P41146-2) [UniParc]FASTAAdd to basket
Also known as: KOR-3D

The sequence of this isoform differs from the canonical sequence as follows:
     74-78: Missing.

Show »
Length:365
Mass (Da):40,048
Checksum:i56D4D8DF0F277242
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00189774 – 78Missing in isoform 2. 1 Publication5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77130 mRNA. Translation: CAA54386.1.
U30185 mRNA. Translation: AAA84913.1.
AF348323 mRNA. Translation: AAK11714.1.
AF126470 mRNA. Translation: AAL54890.1.
AY268428 mRNA. Translation: AAP23195.1.
CR542061 mRNA. Translation: CAG46858.1.
AL121581 Genomic DNA. Translation: CAC17003.2.
AL590548, AL121581 Genomic DNA. Translation: CAD11904.2.
BC038433 mRNA. Translation: AAH38433.1.
L40949 mRNA. Translation: AAA96251.1.
CCDSiCCDS13556.1. [P41146-1]
PIRiS43087.
RefSeqiNP_000904.1. NM_000913.5. [P41146-1]
NP_001186948.1. NM_001200019.1. [P41146-1]
NP_001305782.1. NM_001318853.1.
NP_001305783.1. NM_001318854.1. [P41146-2]
NP_001305784.1. NM_001318855.1.
NP_872588.1. NM_182647.3. [P41146-1]
XP_016883343.1. XM_017027854.1. [P41146-1]
XP_016883344.1. XM_017027855.1. [P41146-2]
UniGeneiHs.2859.
Hs.736944.

Genome annotation databases

EnsembliENST00000336866; ENSP00000336843; ENSG00000125510. [P41146-1]
ENST00000349451; ENSP00000336764; ENSG00000125510. [P41146-1]
ENST00000355631; ENSP00000347848; ENSG00000125510. [P41146-1]
GeneIDi4987.
KEGGihsa:4987.
UCSCiuc002yic.4. human. [P41146-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiOPRX_HUMAN
AccessioniPrimary (citable) accession number: P41146
Secondary accession number(s): Q8TD34, Q8WYH9, Q9H4K4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: September 27, 2017
This is version 173 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Human chromosome 20
    Human chromosome 20: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families