P41146 (OPRX_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nociceptin receptor

Alternative name(s):
Kappa-type 3 opioid receptor
Short name=KOR-3
Orphanin FQ receptor

Gene names

Name:	OPRL1
Synonyms:	OOR, ORL1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	370 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for the neuropeptide nociceptin/orphanin FQ. Has a potential role in modulating a number of brain functions, including instinctive behaviors and emotions. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Induction	By phytohemagglutinin (PHA). Ref.9
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway Traceable author statement PubMed 10965058. Source: ProtInc elevation of cytosolic calcium ion concentration Inferred from genetic interaction PubMed 10571060. Source: UniProtKB opioid receptor signaling pathway Inferred from electronic annotation. Source: GOC sensory perception Traceable author statement PubMed 7566152. Source: ProtInc
Cellular_component	integral to plasma membrane Traceable author statement Ref.1. Source: ProtInc
Molecular_function	G-protein coupled receptor activity Inferred from genetic interaction PubMed 10571060. Source: UniProtKB nociceptin receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P41146-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P41146-2)

Also known as: KOR-3D;

The sequence of this isoform differs from the canonical sequence as follows:
     74-78: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 370

370

Nociceptin receptor

PRO_0000069980

Regions

Topological domain

1 – 50

50

Extracellular Potential

Transmembrane

51 – 77

27

Helical; Name=1; Potential

Topological domain

78 – 87

10

Cytoplasmic Potential

Transmembrane

88 – 109

22

Helical; Name=2; Potential

Topological domain

110 – 124

15

Extracellular Potential

Transmembrane

125 – 146

22

Helical; Name=3; Potential

Topological domain

147 – 165

19

Cytoplasmic Potential

Transmembrane

166 – 188

23

Helical; Name=4; Potential

Topological domain

189 – 211

23

Extracellular Potential

Transmembrane

212 – 236

25

Helical; Name=5; Potential

Topological domain

237 – 264

28

Cytoplasmic Potential

Transmembrane

265 – 288

24

Helical; Name=6; Potential

Topological domain

289 – 300

12

Extracellular Potential

Transmembrane

301 – 322

22

Helical; Name=7; Potential

Topological domain

323 – 370

48

Cytoplasmic Potential

Amino acid modifications

Lipidation

334

1

S-palmitoyl cysteine Potential

Glycosylation

21

1

N-linked (GlcNAc...) Potential

Glycosylation

28

1

N-linked (GlcNAc...) Potential

Glycosylation

39

1

N-linked (GlcNAc...) Potential

Disulfide bond

123 ↔ 200

By similarity

Natural variations

Alternative sequence

74 – 78

5

Missing in isoform 2.

VSP_001897

Secondary structure

1

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370

Helix Strand Turn

Details...

Helix

48 – 77

30

Helix

85 – 101

17

Helix

104 – 113

10

Helix

119 – 153

35

Helix

164 – 188

25

Beta strand

189 – 191

3

Beta strand

200 – 202

3

Helix

208 – 223

16

Helix

225 – 242

18

Helix

252 – 287

36

Helix

295 – 322

28

Helix

324 – 330

7

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 613700C0B4D093BA	FASTA	370	40,693
	10 20 30 40 50 60 MEPLFPAPFW EVIYGSHLQG NLSLLSPNHS LLPPHLLLNA SHGAFLPLGL KVTIVGLYLA 70 80 90 100 110 120 VCVGGLLGNC LVMYVILRHT KMKTATNIYI FNLALADTLV LLTLPFQGTD ILLGFWPFGN 130 140 150 160 170 180 ALCKTVIAID YYNMFTSTFT LTAMSVDRYV AICHPIRALD VRTSSKAQAV NVAIWALASV 190 200 210 220 230 240 VGVPVAIMGS AQVEDEEIEC LVEIPTPQDY WGPVFAICIF LFSFIVPVLV ISVCYSLMIR 250 260 270 280 290 300 RLRGVRLLSG SREKDRNLRR ITRLVLVVVA VFVGCWTPVQ VFVLAQGLGV QPSSETAVAI 310 320 330 340 350 360 LRFCTALGYV NSCLNPILYA FLDENFKACF RKFCCASALR RDVQVSDRVR SIAKDVALAC 370 KTSETVPRPA
	Isoform 2 (KOR-3D) [UniParc]. Checksum: 56D4D8DF0F277242	FASTA	365	40,048
	10 20 30 40 50 60 MEPLFPAPFW EVIYGSHLQG NLSLLSPNHS LLPPHLLLNA SHGAFLPLGL KVTIVGLYLA 70 80 90 100 110 120 VCVGGLLGNC LVMHTKMKTA TNIYIFNLAL ADTLVLLTLP FQGTDILLGF WPFGNALCKT 130 140 150 160 170 180 VIAIDYYNMF TSTFTLTAMS VDRYVAICHP IRALDVRTSS KAQAVNVAIW ALASVVGVPV 190 200 210 220 230 240 AIMGSAQVED EEIECLVEIP TPQDYWGPVF AICIFLFSFI VPVLVISVCY SLMIRRLRGV 250 260 270 280 290 300 RLLSGSREKD RNLRRITRLV LVVVAVFVGC WTPVQVFVLA QGLGVQPSSE TAVAILRFCT 310 320 330 340 350 360 ALGYVNSCLN PILYAFLDEN FKACFRKFCC ASALRRDVQV SDRVRSIAKD VALACKTSET VPRPA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"ORL1, a novel member of the opioid receptor family. Cloning, functional expression and localization." Mollereau C., Parmentier M., Mailleux P., Butour J.-L., Moisand C., Chalon P., Caput D., Vassart G., Meunier C. FEBS Lett. 341:33-38(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Brain stem.
[2]	Lee P.H., Zhu J., Liu-Chen L., Chang K. Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"Nociceptin stimulates neutrophil chemotaxis and recruitment: Inhibition by aspirin-triggered-15-Epi-lipoxin A4." Serhan C.N., Fierro I.M., Chiang N., Pouliot M. J. Immunol. 166:3650-3654(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[4]	"Identification and characterization of a splice variant of human kappa3-related opioid receptor (KOR-3D) gene." Pan Y.-X., Xu J., Pasternak G.W. Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[5]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Warren C.N., Aronstam R.S., Sharma S.V. Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[6]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[7]	"The DNA sequence and comparative analysis of human chromosome 20." Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J. Nature 414:865-871(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
[9]	"Expression of alternate forms of brain opioid 'orphan' receptor mRNA in activated human peripheral blood lymphocytes and lymphocytic cell lines." Wick M.J., Minnerath S.R., Roy S., Ramakrishnan S., Loh H.H. Brain Res. Mol. Brain Res. 32:342-347(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-333 (ISOFORM 1), INDUCTION. Tissue: Lymphocyte.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X77130 mRNA. Translation: CAA54386.1.
U30185 mRNA. Translation: AAA84913.1.
AF348323 mRNA. Translation: AAK11714.1.
AF126470 mRNA. Translation: AAL54890.1.
AY268428 mRNA. Translation: AAP23195.1.
CR542061 mRNA. Translation: CAG46858.1.
AL121581 Genomic DNA. Translation: CAC17003.2.
AL590548, AL121581 Genomic DNA. Translation: CAD11904.2.
BC038433 mRNA. Translation: AAH38433.1.
L40949 mRNA. Translation: AAA96251.1.

IPI

IPI00012736.
IPI00215696.

PIR

S43087.

RefSeq

NP_000904.1. NM_000913.4.
NP_001186948.1. NM_001200019.1.
NP_872588.1. NM_182647.2.

UniGene

Hs.2859.
Hs.736944.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4EA3	X-ray	3.01	A/B	43-339	[»]

ProteinModelPortal

P41146.

ModBase

Search...

Protein-protein interaction databases

IntAct

P41146. 1 interaction.

STRING

9606.ENSP00000336764.

Protein family/group databases

GPCRDB

Search...

PTM databases

PhosphoSite

P41146.

Polymorphism databases

DMDM

730230.

Proteomic databases

PRIDE

P41146.

Protocols and materials databases

DNASU

4987.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000336866; ENSP00000336843; ENSG00000125510.
ENST00000349451; ENSP00000336764; ENSG00000125510.
ENST00000355631; ENSP00000347848; ENSG00000125510.

GeneID

4987.

KEGG

hsa:4987.

UCSC

uc002yic.3. human.
uc002yif.4. human.

Organism-specific databases

CTD

4987.

GeneCards

GC20P062711.

HGNC

HGNC:8155. OPRL1.

MIM

602548. gene.

neXtProt

NX_P41146.

PharmGKB

PA31944.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG299442.

HOGENOM

HOG000230486.

HOVERGEN

HBG106919.

InParanoid

P41146.

KO

K04216.

OMA

VFAICIF.

OrthoDB

EOG4Z62NR.

PhylomeDB

P41146.

Enzyme and pathway databases

Reactome

REACT_111102. Signal Transduction.

Gene expression databases

Bgee

P41146.

CleanEx

HS_OPRL1.

Genevestigator

P41146.

GermOnline

ENSG00000125510. Homo sapiens.

Family and domain databases

InterPro

IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001418. Opioid_rcpt.
IPR001420. X_opioid_rcpt.
[Graphical view]

Pfam

PF00001. 7tm_1. 1 hit.
[Graphical view]

PRINTS

PR00237. GPCRRHODOPSN.
PR00384. OPIOIDR.
PR00547. XOPIOIDR.

PROSITE

PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

P41146.

ChEMBL

CHEMBL2014.

GenomeRNAi

4987.

NextBio

19198.

SOURCE

Search...

Entry information

Entry name

OPRX_HUMAN

Accession

Primary (citable) accession number: P41146
Secondary accession number(s): Q8TD34, Q8WYH9, Q9H4K4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 132 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.