Kappa-type opioid receptor - Homo sapiens (Human)

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P41145 (OPRK_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 122. History...

Clusters with 100%, 90%, 50% identity |

Documents (7) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Kappa-type opioid receptor
Short name=K-OR-1
Short name=KOR-1

Gene names

Name:	OPRK1
Synonyms:	OPRK

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	380 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Receptor for dynorphins. May play a role in arousal and regulation of autonomic and neuroendocrine functions.
Subunit structure	Interacts with SLC9A3R1. Interacts with GABARAPL1. Ref.8 Ref.9
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway Traceable author statement Ref.2. Source: ProtInc behavior Traceable author statement Ref.2. Source: ProtInc defense response to virus Inferred from direct assay PubMed 8755601. Source: UniProtKB immune response Inferred from direct assay PubMed 8755601. Source: UniProtKB phospholipase C-activating G-protein coupled receptor signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB sensory perception Traceable author statement Ref.2. Source: ProtInc synaptic transmission Traceable author statement Ref.2. Source: ProtInc
Cellular_component	integral to plasma membrane Traceable author statement Ref.2. Source: ProtInc
Molecular_function	dynorphin receptor activity Inferred from direct assay PubMed 8755601. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GABARAPL1	Q9H0R8	5	EBI-925028,EBI-3464833

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 380

380

Kappa-type opioid receptor

PRO_0000069967

Regions

Topological domain

1 – 58

Extracellular Potential

Transmembrane

59 – 85

Helical; Name=1; Potential

Topological domain

86 – 95

Cytoplasmic Potential

Transmembrane

96 – 117

Helical; Name=2; Potential

Topological domain

118 – 132

Extracellular Potential

Transmembrane

133 – 154

Helical; Name=3; Potential

Topological domain

155 – 173

Cytoplasmic Potential

Transmembrane

174 – 196

Helical; Name=4; Potential

Topological domain

197 – 222

Extracellular Potential

Transmembrane

223 – 247

Helical; Name=5; Potential

Topological domain

248 – 275

Cytoplasmic Potential

Transmembrane

276 – 299

Helical; Name=6; Potential

Topological domain

300 – 311

Extracellular Potential

Transmembrane

312 – 333

Helical; Name=7; Potential

Topological domain

334 – 380

Cytoplasmic Potential

Amino acid modifications

Lipidation

345

S-palmitoyl cysteine Potential

Glycosylation

N-linked (GlcNAc...) Ref.10

Glycosylation

N-linked (GlcNAc...) Ref.10

Disulfide bond

131 ↔ 210

Ref.11

Natural variations

Natural variant

374

D → N.
Corresponds to variant rs9282808 [ dbSNP | Ensembl ].

VAR_028067

Experimental info

Sequence conflict

D → E Ref.1

Sequence conflict

D → E Ref.4

Secondary structure

380

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P41145 [UniParc].

Last modified October 17, 2006. Version 2.
Checksum: 3DA6E9F90FB48825
Show »

FASTA

380

42,645

        10         20         30         40         50         60 
MDSPIQIFRG EPGPTCAPSA CLPPNSSAWF PGWAEPDSNG SAGSEDAQLE PAHISPAIPV 

        70         80         90        100        110        120 
IITAVYSVVF VVGLVGNSLV MFVIIRYTKM KTATNIYIFN LALADALVTT TMPFQSTVYL 

       130        140        150        160        170        180 
MNSWPFGDVL CKIVISIDYY NMFTSIFTLT MMSVDRYIAV CHPVKALDFR TPLKAKIINI 

       190        200        210        220        230        240 
CIWLLSSSVG ISAIVLGGTK VREDVDVIEC SLQFPDDDYS WWDLFMKICV FIFAFVIPVL 

       250        260        270        280        290        300 
IIIVCYTLMI LRLKSVRLLS GSREKDRNLR RITRLVLVVV AVFVVCWTPI HIFILVEALG 

       310        320        330        340        350        360 
STSHSTAALS SYYFCIALGY TNSSLNPILY AFLDENFKRC FRDFCFPLKM RMERQSTSRV 

       370        380 
RNTVQDPAYL RDIDGMNKPV

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of a human kappa opioid receptor cDNA from placenta." Mansson E., Bare L.A., Yang D. Biochem. Biophys. Res. Commun. 202:1431-1437(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Placenta.
[2]	"Kappa-opioid receptor in humans: cDNA and genomic cloning, chromosomal assignment, functional expression, pharmacology, and expression pattern in the central nervous system." Simonin F., Gaveriaus-Ruff C., Befort K., Lannes B., Micheletti G., Mattei M.-G., Charon G., Bloch B., Kieffer B. Proc. Natl. Acad. Sci. U.S.A. 92:7006-7010(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Placenta.
[3]	"Cloning of a human kappa opioid receptor from the brain." Zhu J., Chen C., Xue J.-C., Kunapuli S., Deriel J.K., Liu-Chen L.-Y. Life Sci. 56:PL201-PL207(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[4]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[6]	"Human kappa opiate receptor second extracellular loop elevates dynorphin's affinity for human mu/kappa chimeras." Wang J.B., Johnson P.S., Wu J.M., Wang W.F., Uhl G.R. J. Biol. Chem. 269:25966-25969(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 136-279. Tissue: Brain.
[7]	"Mapping of the human kappa opioid receptor gene to chromosome 8q11.2-q12: no evidence for multiple kappa opioid receptor genes." Grandy D.K. Submitted (DEC-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 132-203.
[8]	"Ezrin-radixin-moesin-binding phosphoprotein-50/Na+/H+ exchanger regulatory factor (EBP50/NHERF) blocks U50,488H-induced down-regulation of the human kappa opioid receptor by enhancing its recycling rate." Li J.-G., Chen C., Liu-Chen L.-Y. J. Biol. Chem. 277:27545-27552(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH SLC9A3R1.
[9]	"GEC1 interacts with the kappa opioid receptor and enhances expression of the receptor." Chen C., Li J.-G., Chen Y., Huang P., Wang Y., Liu-Chen L.-Y. J. Biol. Chem. 281:7983-7993(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH GABARAPL1.
[10]	"N-glycosylation of the human kappa opioid receptor enhances its stability but slows its trafficking along the biosynthesis pathway." Li J.G., Chen C., Liu-Chen L.Y. Biochemistry 46:10960-10970(2007) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION AT ASN-25 AND ASN-39.
[11]	"Structure of the human kappa-opioid receptor in complex with JDTic." Wu H., Wacker D., Mileni M., Katritch V., Han G.W., Vardy E., Liu W., Thompson A.A., Huang X.P., Carroll F.I., Mascarella S.W., Westkaemper R.B., Mosier P.D., Roth B.L., Cherezov V., Stevens R.C. Nature 485:327-332(2012) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS) OF 43-358 IN COMPLEX WITH ANTAGONIST, DISULFIDE BOND.
+	Additional computationally mapped references.

Web resources

Wikipedia

Kappa opioid receptor entry

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U11053 mRNA. Translation: AAA20985.1.
U17298 mRNA. Translation: AAC50158.1.
L37362 mRNA. Translation: AAA63906.1.
AF498922 mRNA. Translation: AAM21070.1.
BC099912 mRNA. Translation: AAH99912.1.
L36130 mRNA. Translation: AAA63646.1.
U16860 Genomic DNA. Translation: AAA56758.1.

IPI

IPI00012735.

PIR

JC2338.

RefSeq

NP_000903.2. NM_000912.3.

UniGene

Hs.106795.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2A0D	model	-	A	1-380	[»]
2IQN	model	-	A	55-348	[»]
4DJH	X-ray	2.90	A/B	43-358	[»]

ProteinModelPortal

P41145.

ModBase

Search...

Protein-protein interaction databases

IntAct

P41145. 3 interactions.

MINT

MINT-236997.

STRING

9606.ENSP00000265572.

Protein family/group databases

GPCRDB

Search...

PTM databases

PhosphoSite

P41145.

Polymorphism databases

DMDM

116242691.

Proteomic databases

PaxDb

P41145.

PRIDE

P41145.

Protocols and materials databases

DNASU

4986.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000265572; ENSP00000265572; ENSG00000082556.
ENST00000520287; ENSP00000429706; ENSG00000082556.

GeneID

4986.

KEGG

hsa:4986.

UCSC

uc003xrh.1. human.

Organism-specific databases

CTD

4986.

GeneCards

GC08M054138.

HGNC

HGNC:8154. OPRK1.

HPA

CAB022595.

MIM

165196. gene.

neXtProt

NX_P41145.

PharmGKB

PA31943.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG317066.

HOGENOM

HOG000230486.

HOVERGEN

HBG106919.

InParanoid

P41145.

K04214.

OMA

DVDVIEC.

PhylomeDB

P41145.

Enzyme and pathway databases

Reactome

REACT_111102. Signal Transduction.

Gene expression databases

ArrayExpress

P41145.

Bgee

P41145.

CleanEx

HS_OPRK1.

Genevestigator

P41145.

GermOnline

ENSG00000082556. Homo sapiens.

Family and domain databases

InterPro

IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR000452. Kappa_opi_rcpt.
IPR001418. Opioid_rcpt.
[Graphical view]

Pfam

PF00001. 7tm_1. 1 hit.
[Graphical view]

PRINTS

PR00237. GPCRRHODOPSN.
PR00532. KAPPAOPIOIDR.
PR00384. OPIOIDR.

PROSITE

PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

P41145.

ChEMBL

CHEMBL237.

DrugBank

DB00921. Buprenorphine.
DB00611. Butorphanol.
DB00907. Cocaine.
DB00318. Codeine.
DB01209. Dezocine.
DB00956. Hydrocodone.
DB00327. Hydromorphone.
DB00454. Meperidine.
DB00370. Mirtazapine.
DB00295. Morphine.
DB00844. Nalbuphine.
DB00704. Naltrexone.
DB00497. Oxycodone.
DB00652. Pentazocine.
DB00647. Propoxyphene.
DB00193. Tramadol.

GenomeRNAi

4986.

NextBio

19194.

SOURCE

Search...

Entry information

Entry name

OPRK_HUMAN

Accession

Primary (citable) accession number: P41145
Secondary accession number(s): Q499G4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	October 17, 2006
Last modified:	May 1, 2013
This is version 122 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

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Human chromosome 8: entries, gene names and cross-references to MIM

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List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Natural variations

Experimental info

Secondary structure

Sequences

References

Web resources

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Polymorphism databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents