P41145 (OPRK_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Kappa-type opioid receptor
Short name=K-OR-1
Short name=KOR-1

Gene names

Name:	OPRK1
Synonyms:	OPRK

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	380 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Receptor for dynorphins. May play a role in arousal and regulation of autonomic and neuroendocrine functions.
Subunit structure	Interacts with SLC9A3R1. Interacts with GABARAPL1. Ref.8 Ref.9
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Uncategorized	kappa-opioid receptor activity Traceable author statement. Source: ProtInc
Biological process	behavior Traceable author statement. Source: ProtInc immune response Traceable author statement. Source: ProtInc inhibition of adenylate cyclase activity by G-protein signaling pathway Traceable author statement. Source: ProtInc sensory perception Traceable author statement. Source: ProtInc synaptic transmission Traceable author statement. Source: ProtInc viral genome replication Traceable author statement. Source: ProtInc
Cellular component	integral to plasma membrane Traceable author statement. Source: ProtInc
Molecular function	opioid receptor activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction Ref.9. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GABARAPL1	Q9H0R8	5	EBI-925028,EBI-3464833

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 380

380

Kappa-type opioid receptor

PRO_0000069967

Regions

Topological domain

1 – 58

58

Extracellular Potential

Transmembrane

59 – 85

27

Helical; Name=1; Potential

Topological domain

86 – 95

10

Cytoplasmic Potential

Transmembrane

96 – 117

22

Helical; Name=2; Potential

Topological domain

118 – 132

15

Extracellular Potential

Transmembrane

133 – 154

22

Helical; Name=3; Potential

Topological domain

155 – 173

19

Cytoplasmic Potential

Transmembrane

174 – 196

23

Helical; Name=4; Potential

Topological domain

197 – 222

26

Extracellular Potential

Transmembrane

223 – 247

25

Helical; Name=5; Potential

Topological domain

248 – 275

28

Cytoplasmic Potential

Transmembrane

276 – 299

24

Helical; Name=6; Potential

Topological domain

300 – 311

12

Extracellular Potential

Transmembrane

312 – 333

22

Helical; Name=7; Potential

Topological domain

334 – 380

47

Cytoplasmic Potential

Amino acid modifications

Lipidation

345

1

S-palmitoyl cysteine Potential

Glycosylation

25

1

N-linked (GlcNAc...) Ref.10

Glycosylation

39

1

N-linked (GlcNAc...) Ref.10

Disulfide bond

131 ↔ 210

By similarity

Natural variations

Natural variant

374

1

D → N.
Corresponds to variant rs9282808 [ dbSNP | Ensembl ].

VAR_028067

Experimental info

Sequence conflict

2

1

D → E Ref.1

Sequence conflict

2

1

D → E Ref.4

Secondary structure

1

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380

Helix Strand Turn

Details...

Helix

57 – 86

30

Helix

93 – 119

27

Turn

120 – 122

3

Turn

125 – 127

3

Helix

128 – 160

33

Turn

164 – 166

3

Helix

172 – 194

23

Beta strand

201 – 204

4

Turn

205 – 208

4

Beta strand

209 – 212

4

Helix

215 – 218

4

Beta strand

221 – 223

3

Helix

224 – 234

11

Helix

236 – 258

23

Helix

263 – 286

24

Helix

288 – 300

13

Helix

309 – 319

11

Helix

322 – 333

12

Helix

335 – 341

7

Helix

342 – 345

4

Sequences

	Sequence		Length	Mass (Da)
	P41145 [UniParc]. Last modified October 17, 2006. Version 2. Checksum: 3DA6E9F90FB48825	FASTA	380	42,645
	10 20 30 40 50 60 MDSPIQIFRG EPGPTCAPSA CLPPNSSAWF PGWAEPDSNG SAGSEDAQLE PAHISPAIPV 70 80 90 100 110 120 IITAVYSVVF VVGLVGNSLV MFVIIRYTKM KTATNIYIFN LALADALVTT TMPFQSTVYL 130 140 150 160 170 180 MNSWPFGDVL CKIVISIDYY NMFTSIFTLT MMSVDRYIAV CHPVKALDFR TPLKAKIINI 190 200 210 220 230 240 CIWLLSSSVG ISAIVLGGTK VREDVDVIEC SLQFPDDDYS WWDLFMKICV FIFAFVIPVL 250 260 270 280 290 300 IIIVCYTLMI LRLKSVRLLS GSREKDRNLR RITRLVLVVV AVFVVCWTPI HIFILVEALG 310 320 330 340 350 360 STSHSTAALS SYYFCIALGY TNSSLNPILY AFLDENFKRC FRDFCFPLKM RMERQSTSRV 370 380 RNTVQDPAYL RDIDGMNKPV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of a human kappa opioid receptor cDNA from placenta." Mansson E., Bare L.A., Yang D. Biochem. Biophys. Res. Commun. 202:1431-1437(1994) [PubMed: 8060324] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Placenta.
[2]	"Kappa-opioid receptor in humans: cDNA and genomic cloning, chromosomal assignment, functional expression, pharmacology, and expression pattern in the central nervous system." Simonin F., Gaveriaus-Ruff C., Befort K., Lannes B., Micheletti G., Mattei M.-G., Charon G., Bloch B., Kieffer B. Proc. Natl. Acad. Sci. U.S.A. 92:7006-7010(1995) [PubMed: 7624359] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Placenta.
[3]	"Cloning of a human kappa opioid receptor from the brain." Zhu J., Chen C., Xue J.-C., Kunapuli S., Deriel J.K., Liu-Chen L.-Y. Life Sci. 56:PL201-PL207(1995) [PubMed: 7869844] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[4]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[6]	"Human kappa opiate receptor second extracellular loop elevates dynorphin's affinity for human mu/kappa chimeras." Wang J.B., Johnson P.S., Wu J.M., Wang W.F., Uhl G.R. J. Biol. Chem. 269:25966-25969(1994) [PubMed: 7929306] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 136-279. Tissue: Brain.
[7]	"Mapping of the human kappa opioid receptor gene to chromosome 8q11.2-q12: no evidence for multiple kappa opioid receptor genes." Grandy D.K. Submitted (DEC-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 132-203.
[8]	"Ezrin-radixin-moesin-binding phosphoprotein-50/Na+/H+ exchanger regulatory factor (EBP50/NHERF) blocks U50,488H-induced down-regulation of the human kappa opioid receptor by enhancing its recycling rate." Li J.-G., Chen C., Liu-Chen L.-Y. J. Biol. Chem. 277:27545-27552(2002) [PubMed: 12004055] [Abstract] Cited for: INTERACTION WITH SLC9A3R1.
[9]	"GEC1 interacts with the kappa opioid receptor and enhances expression of the receptor." Chen C., Li J.-G., Chen Y., Huang P., Wang Y., Liu-Chen L.-Y. J. Biol. Chem. 281:7983-7993(2006) [PubMed: 16431922] [Abstract] Cited for: INTERACTION WITH GABARAPL1.
[10]	"N-glycosylation of the human kappa opioid receptor enhances its stability but slows its trafficking along the biosynthesis pathway." Li J.G., Chen C., Liu-Chen L.Y. Biochemistry 46:10960-10970(2007) [PubMed: 17711303] [Abstract] Cited for: GLYCOSYLATION AT ASN-25 AND ASN-39.
+	Additional computationally mapped references.

Web resources

Wikipedia

Kappa opioid receptor entry

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U11053 mRNA. Translation: AAA20985.1.
U17298 mRNA. Translation: AAC50158.1.
L37362 mRNA. Translation: AAA63906.1.
AF498922 mRNA. Translation: AAM21070.1.
BC099912 mRNA. Translation: AAH99912.1.
L36130 mRNA. Translation: AAA63646.1.
U16860 Genomic DNA. Translation: AAA56758.1.

IPI

IPI00012735.

PIR

JC2338.

RefSeq

NP_000903.2. NM_000912.3.

UniGene

Hs.106795.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2A0D	model	-	A	1-380	[»]
2IQN	model	-	A	55-348	[»]

ProteinModelPortal

P41145.

SMR

P41145. Positions 58-348.

ModBase

Search...

Protein-protein interaction databases

IntAct

P41145. 3 interactions.

MINT

MINT-236997.

STRING

P41145.

Protein family/group databases

GPCRDB

Search...

PTM databases

PhosphoSite

P41145.

Polymorphism databases

DMDM

116242691.

Proteomic databases

PRIDE

P41145.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000265572; ENSP00000265572; ENSG00000082556.

GeneID

4986.

KEGG

hsa:4986.

UCSC

uc003xrh.1. human.

Organism-specific databases

CTD

4986.

GeneCards

GC08M054191.

H-InvDB

HIX0034286.

HGNC

HGNC:8154. OPRK1.

HPA

CAB022595.

MIM

165196. gene.

neXtProt

NX_P41145.

PharmGKB

PA31943.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG08919.

GeneTree

ENSGT00600000084010.

HOGENOM

HBG715643.

HOVERGEN

HBG106919.

InParanoid

P41145.

OMA

RNTVQDP.

PhylomeDB

P41145.

Enzyme and pathway databases

Reactome

REACT_111102. Signal Transduction.

Gene expression databases

ArrayExpress

P41145.

Bgee

P41145.

CleanEx

HS_OPRK1.

Genevestigator

P41145.

GermOnline

ENSG00000082556. Homo sapiens.

Family and domain databases

InterPro

IPR000276. 7TM_GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_supfam.
IPR000452. Kappa_opi_rcpt.
IPR001418. Opioid_rcpt.
[Graphical view]

KO

K04214.

Pfam

PF00001. 7tm_1. 1 hit.
[Graphical view]

PRINTS

PR00237. GPCRRHODOPSN.
PR00532. KAPPAOPIOIDR.
PR00384. OPIOIDR.

PROSITE

PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

DrugBank

DB00921. Buprenorphine.
DB00611. Butorphanol.
DB00907. Cocaine.
DB00318. Codeine.
DB01209. Dezocine.
DB00956. Hydrocodone.
DB00327. Hydromorphone.
DB00454. Meperidine.
DB00370. Mirtazapine.
DB00295. Morphine.
DB00844. Nalbuphine.
DB00704. Naltrexone.
DB00497. Oxycodone.
DB00652. Pentazocine.
DB00647. Propoxyphene.
DB00193. Tramadol.

NextBio

19194.

SOURCE

Search...

Entry information

Entry name

OPRK_HUMAN

Accession

Primary (citable) accession number: P41145
Secondary accession number(s): Q499G4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	October 17, 2006
Last modified:	January 25, 2012
This is version 110 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.