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P41143 (OPRD_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 133. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (7) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Delta-type opioid receptor

Short name=D-OR-1
Short name=DOR-1
Gene names
Name:OPRD1
Synonyms:OPRD
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length372 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

G-protein coupled receptor that functions as receptor for endogenous enkephalins and for a subset of other opioids. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Plays a role in the perception of pain and in opiate-mediated analgesia. Plays a role in developing analgesic tolerance to morphine. Ref.1 Ref.3 Ref.8

Subunit structure

May form homooligomers. Interacts with GPRASP1. Ref.6 Ref.7 Ref.8

Subcellular location

Cell membrane; Multi-pass membrane protein Ref.1 Ref.3 Ref.8.

Tissue specificity

Detected in oocytes (at protein level). Detected in brain cortex, hypothalamus, hippocampus and olfactory bulb. Detected in oocytes. Ref.3 Ref.9

Post-translational modification

N-glycosylated. Ref.8

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   Coding sequence diversityPolymorphism
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
Lipoprotein
Palmitate
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processG-protein coupled receptor signaling pathway

Inferred from direct assay PubMed 16325578. Source: UniProtKB

G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger

Traceable author statement Ref.3. Source: ProtInc

adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

adult locomotory behavior

Inferred from electronic annotation. Source: Ensembl

cellular response to growth factor stimulus

Inferred from electronic annotation. Source: Ensembl

immune response

Traceable author statement PubMed 9548483. Source: ProtInc

neuropeptide signaling pathway

Inferred from electronic annotation. Source: Ensembl

opioid receptor signaling pathway

Inferred from mutant phenotype PubMed 9548483. Source: UniProtKB

phospholipase C-activating G-protein coupled receptor signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

protein import into nucleus, translocation

Inferred from direct assay PubMed 16325578. Source: UniProtKB

regulation of calcium ion transport

Inferred from electronic annotation. Source: Ensembl

regulation of sensory perception of pain

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentaxon terminus

Inferred from electronic annotation. Source: Ensembl

cytoplasm

Inferred from electronic annotation. Source: Ensembl

dendrite membrane

Inferred from electronic annotation. Source: Ensembl

integral component of plasma membrane

Traceable author statement Ref.3. Source: ProtInc

intrinsic component of plasma membrane

Inferred from direct assay Ref.8. Source: UniProtKB

membrane raft

Inferred from electronic annotation. Source: Ensembl

plasma membrane

Traceable author statement. Source: Reactome

postsynaptic membrane

Inferred from electronic annotation. Source: Ensembl

vesicle

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionenkephalin receptor activity

Inferred from mutant phenotype PubMed 9548483. Source: UniProtKB

opioid receptor activity

Inferred from direct assay Ref.8. Source: UniProtKB

protein binding

Inferred from physical interaction Ref.7PubMed 20528919. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ATP2A2P116072EBI-2624456,EBI-8004986From a different organism.
ATP2A2P166153EBI-2624456,EBI-358933
CANXP278242EBI-2624456,EBI-355947
GPRASP1Q5JY772EBI-2624456,EBI-2514717

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 372372Delta-type opioid receptor
PRO_0000069962

Regions

Topological domain1 – 4747Extracellular By similarity
Transmembrane48 – 7528Helical; Name=1; By similarity
Topological domain76 – 8510Cytoplasmic By similarity
Transmembrane86 – 11025Helical; Name=2; By similarity
Topological domain111 – 12212Extracellular By similarity
Transmembrane123 – 14422Helical; Name=3; By similarity
Topological domain145 – 16319Cytoplasmic By similarity
Transmembrane164 – 18623Helical; Name=4; By similarity
Topological domain187 – 20620Extracellular By similarity
Transmembrane207 – 23832Helical; Name=5; By similarity
Topological domain239 – 26123Cytoplasmic By similarity
Transmembrane262 – 28423Helical; Name=6; By similarity
Topological domain285 – 29915Extracellular By similarity
Transmembrane300 – 32122Helical; Name=7; By similarity
Topological domain322 – 37251Cytoplasmic By similarity

Amino acid modifications

Lipidation3331S-palmitoyl cysteine Potential
Glycosylation181N-linked (GlcNAc...) Potential
Glycosylation331N-linked (GlcNAc...) Potential
Disulfide bond121 ↔ 198 By similarity

Natural variations

Natural variant271C → F Improved maturation and increased expression at the cell surface. Ref.1 Ref.4 Ref.8 Ref.10
Corresponds to variant rs1042114 [ dbSNP | Ensembl ].
VAR_012083

Secondary structure

............................... 372
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P41143 [UniParc].

Last modified November 2, 2010. Version 4.
Checksum: 9D483FC39BA2BAE4

FASTA37240,369
        10         20         30         40         50         60 
MEPAPSAGAE LQPPLFANAS DAYPSACPSA GANASGPPGA RSASSLALAI AITALYSAVC 

        70         80         90        100        110        120 
AVGLLGNVLV MFGIVRYTKM KTATNIYIFN LALADALATS TLPFQSAKYL METWPFGELL 

       130        140        150        160        170        180 
CKAVLSIDYY NMFTSIFTLT MMSVDRYIAV CHPVKALDFR TPAKAKLINI CIWVLASGVG 

       190        200        210        220        230        240 
VPIMVMAVTR PRDGAVVCML QFPSPSWYWD TVTKICVFLF AFVVPILIIT VCYGLMLLRL 

       250        260        270        280        290        300 
RSVRLLSGSK EKDRSLRRIT RMVLVVVGAF VVCWAPIHIF VIVWTLVDID RRDPLVVAAL 

       310        320        330        340        350        360 
HLCIALGYAN SSLNPVLYAF LDENFKRCFR QLCRKPCGRP DPSSFSRARE ATARERVTAC 

       370 
TPSDGPGGGA AA 

« Hide

References

« Hide 'large scale' references
[1]"Identification of a human delta opioid receptor: cloning and expression."
Knapp R.J., Malatynska E., Fang L., Li X., Babin E., Nguyen M., Santoro G., Varga E.V., Hruby V.J., Roeske W.R., Yamamura H.I.
Life Sci. 54:PL463-PL469(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, VARIANT PHE-27.
Tissue: Brain cortex and Corpus striatum.
[2]Knapp R.J.
Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO 40-41; 348 AND 370.
[3]"The human delta-opioid receptor: genomic organization, cDNA cloning, functional expression, and distribution in human brain."
Simonin F., Befort K., Gaveriaux-Ruff C., Matthes H., Nappey V., Lannes B., Micheletti G., Kieffer B.
Mol. Pharmacol. 46:1015-1021(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[4]"Isolation of cDNA coding for human opioid receptor, delta 1 (OPRD1)."
Kaighin V.A., Martin A.L., Aronstam R.S.
Submitted (JUL-2008) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT PHE-27.
Tissue: Kidney.
[5]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"Modulation of postendocytic sorting of G protein-coupled receptors."
Whistler J.L., Enquist J., Marley A., Fong J., Gladher F., Tsuruda P., Murray S.R., Von Zastrow M.
Science 297:615-620(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH GPRASP1.
[7]"Identification of a novel family of G protein-coupled receptor associated sorting proteins."
Simonin F., Karcher P., Boeuf J.J.-M., Matifas A., Kieffer B.L.
J. Neurochem. 89:766-775(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH GPRASP1.
[8]"Cys-27 variant of human delta-opioid receptor modulates maturation and cell surface delivery of Phe-27 variant via heteromerization."
Leskela T.T., Lackman J.J., Vierimaa M.M., Kobayashi H., Bouvier M., Petaja-Repo U.E.
J. Biol. Chem. 287:5008-5020(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, GLYCOSYLATION, SUBUNIT, CHARACTERIZATION OF VARIANT PHE-27.
[9]"Expression and localization of opioid receptors during the maturation of human oocytes."
Agirregoitia E., Peralta L., Mendoza R., Exposito A., Ereno E.D., Matorras R., Agirregoitia N.
Reprod. BioMed. Online 24:550-557(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[10]"Variant detection at the delta opioid receptor (OPRD1) locus and population genetics of a novel variant affecting protein sequence."
Gelernter J., Kranzler H.R.
Hum. Genet. 107:86-88(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT PHE-27.
[11]"Opioid receptor random mutagenesis reveals a mechanism for G protein-coupled receptor activation."
Decaillot F.M., Befort K., Filliol D., Yue S., Walker P., Kieffer B.L.
Nat. Struct. Biol. 10:629-636(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: 3D-STRUCTURE MODELING.
+Additional computationally mapped references.

Web resources

Wikipedia

Delta opioid receptor entry

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U07882 mRNA. Translation: AAA18789.2.
U10504 mRNA. Translation: AAA83426.1.
EU883570 mRNA. Translation: ACG60644.1.
AL009181 Genomic DNA. Translation: CAA15671.1.
CCDSCCDS329.1.
PIRI38532.
RefSeqNP_000902.3. NM_000911.3.
UniGeneHs.372.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1OZCmodel-A1-372[»]
2IQMmodel-A45-336[»]
4N6HX-ray1.80A36-338[»]
ProteinModelPortalP41143.
SMRP41143. Positions 36-338.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid111030. 11 interactions.
IntActP41143. 4 interactions.
MINTMINT-7889289.
STRING9606.ENSP00000234961.

Chemistry

BindingDBP41143.
ChEMBLCHEMBL2095149.
DrugBankDB00611. Butorphanol.
DB00318. Codeine.
DB00813. Fentanyl.
DB00956. Hydrocodone.
DB00327. Hydromorphone.
DB00836. Loperamide.
DB00295. Morphine.
DB00844. Nalbuphine.
DB01183. Naloxone.
DB00704. Naltrexone.
DB00497. Oxycodone.
DB01100. Pimozide.
DB00647. Propoxyphene.
GuidetoPHARMACOLOGY317.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP41143.

Polymorphism databases

DMDM311033488.

Proteomic databases

PaxDbP41143.
PRIDEP41143.

Protocols and materials databases

DNASU4985.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000234961; ENSP00000234961; ENSG00000116329.
GeneID4985.
KEGGhsa:4985.
UCSCuc001brf.1. human.

Organism-specific databases

CTD4985.
GeneCardsGC01P029144.
HGNCHGNC:8153. OPRD1.
HPAHPA013744.
MIM165195. gene.
neXtProtNX_P41143.
PharmGKBPA31942.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG241795.
HOGENOMHOG000230486.
HOVERGENHBG106919.
InParanoidP41143.
KOK04213.
OMALCRKPCG.
OrthoDBEOG7BKCVQ.
PhylomeDBP41143.
TreeFamTF315737.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.
SignaLinkP41143.

Gene expression databases

BgeeP41143.
CleanExHS_OPRD1.
GenevestigatorP41143.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR000321. Delta_opi_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001418. Opioid_rcpt.
[Graphical view]
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00525. DELTAOPIOIDR.
PR00237. GPCRRHODOPSN.
PR00384. OPIOIDR.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWiki%CE%94-opioid_receptor.
GenomeRNAi4985.
NextBio19190.
PROP41143.
SOURCESearch...

Entry information

Entry nameOPRD_HUMAN
AccessionPrimary (citable) accession number: P41143
Secondary accession number(s): B5B0B8
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 2, 2010
Last modified: July 9, 2014
This is version 133 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries