P41143 (OPRD_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Delta-type opioid receptor
Short name=D-OR-1
Short name=DOR-1

Gene names

Name:	OPRD1
Synonyms:	OPRD

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Highly stereoselective. receptor for enkephalins.
Subunit structure	Interacts with GPRASP1. Ref.6 Ref.7
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger Traceable author statement Ref.3. Source: ProtInc adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway Inferred from electronic annotation. Source: Compara adult locomotory behavior Inferred from electronic annotation. Source: Compara cellular response to growth factor stimulus Inferred from electronic annotation. Source: Compara immune response Traceable author statement PubMed 9548483. Source: ProtInc neuropeptide signaling pathway Inferred from electronic annotation. Source: Compara phospholipase C-activating G-protein coupled receptor signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB protein import into nucleus, translocation Inferred from direct assay PubMed 16325578. Source: UniProtKB regulation of calcium ion transport Inferred from electronic annotation. Source: Compara regulation of sensory perception of pain Inferred from electronic annotation. Source: Compara
Cellular_component	axon terminus Inferred from electronic annotation. Source: Compara cytoplasm Inferred from electronic annotation. Source: Compara dendrite membrane Inferred from electronic annotation. Source: Compara integral to plasma membrane Traceable author statement Ref.3. Source: ProtInc membrane raft Inferred from electronic annotation. Source: Compara postsynaptic membrane Inferred from electronic annotation. Source: Compara vesicle Inferred from electronic annotation. Source: Compara
Molecular_function	enkephalin receptor activity Inferred from mutant phenotype PubMed 9548483. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GPRASP1	Q5JY77	2	EBI-2624456,EBI-2514717

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 372

372

Delta-type opioid receptor

PRO_0000069962

Regions

Topological domain

1 – 45

45

Extracellular Potential

Transmembrane

46 – 75

30

Helical; Name=1; Potential

Topological domain

76 – 84

9

Cytoplasmic Potential

Transmembrane

85 – 102

18

Helical; Name=2; Potential

Topological domain

103 – 124

22

Extracellular Potential

Transmembrane

125 – 144

20

Helical; Name=3; Potential

Topological domain

145 – 174

30

Cytoplasmic Potential

Transmembrane

175 – 190

16

Helical; Name=4; Potential

Topological domain

191 – 215

25

Extracellular Potential

Transmembrane

216 – 238

23

Helical; Name=5; Potential

Topological domain

239 – 261

23

Cytoplasmic Potential

Transmembrane

262 – 284

23

Helical; Name=6; Potential

Topological domain

285 – 293

9

Extracellular Potential

Transmembrane

294 – 310

17

Helical; Name=7; Potential

Topological domain

311 – 372

62

Cytoplasmic Potential

Amino acid modifications

Lipidation

333

1

S-palmitoyl cysteine Potential

Glycosylation

18

1

N-linked (GlcNAc...) Potential

Glycosylation

33

1

N-linked (GlcNAc...) Potential

Disulfide bond

121 ↔ 198

By similarity

Natural variations

Natural variant

27

1

C → F. Ref.1 Ref.4 Ref.8
Corresponds to variant rs1042114 [ dbSNP | Ensembl ].

VAR_012083

Secondary structure

1

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372

Helix Strand Turn

Details...

Helix

48 – 76

29

Helix

83 – 101

19

Helix

103 – 108

6

Turn

109 – 112

4

Helix

117 – 150

34

Helix

162 – 180

19

Helix

181 – 183

3

Turn

184 – 186

3

Helix

213 – 221

9

Helix

223 – 236

14

Turn

237 – 239

3

Beta strand

249 – 253

5

Helix

254 – 283

30

Beta strand

291 – 293

3

Helix

297 – 307

11

Helix

310 – 321

12

Helix

323 – 328

6

Helix

329 – 332

4

Sequences

	Sequence		Length	Mass (Da)
	P41143 [UniParc]. Last modified November 2, 2010. Version 4. Checksum: 9D483FC39BA2BAE4	FASTA	372	40,369
	10 20 30 40 50 60 MEPAPSAGAE LQPPLFANAS DAYPSACPSA GANASGPPGA RSASSLALAI AITALYSAVC 70 80 90 100 110 120 AVGLLGNVLV MFGIVRYTKM KTATNIYIFN LALADALATS TLPFQSAKYL METWPFGELL 130 140 150 160 170 180 CKAVLSIDYY NMFTSIFTLT MMSVDRYIAV CHPVKALDFR TPAKAKLINI CIWVLASGVG 190 200 210 220 230 240 VPIMVMAVTR PRDGAVVCML QFPSPSWYWD TVTKICVFLF AFVVPILIIT VCYGLMLLRL 250 260 270 280 290 300 RSVRLLSGSK EKDRSLRRIT RMVLVVVGAF VVCWAPIHIF VIVWTLVDID RRDPLVVAAL 310 320 330 340 350 360 HLCIALGYAN SSLNPVLYAF LDENFKRCFR QLCRKPCGRP DPSSFSRARE ATARERVTAC 370 TPSDGPGGGA AA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a human delta opioid receptor: cloning and expression." Knapp R.J., Malatynska E., Fang L., Li X., Babin E., Nguyen M., Santoro G., Varga E.V., Hruby V.J., Roeske W.R., Yamamura H.I. Life Sci. 54:PL463-PL469(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT PHE-27. Tissue: Brain cortex and Corpus striatum.
[2]	Knapp R.J. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 40-41; 348 AND 370.
[3]	"The human delta-opioid receptor: genomic organization, cDNA cloning, functional expression, and distribution in human brain." Simonin F., Befort K., Gaveriaux-Ruff C., Matthes H., Nappey V., Lannes B., Micheletti G., Kieffer B. Mol. Pharmacol. 46:1015-1021(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Isolation of cDNA coding for human opioid receptor, delta 1 (OPRD1)." Kaighin V.A., Martin A.L., Aronstam R.S. Submitted (JUL-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT PHE-27. Tissue: Kidney.
[5]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"Modulation of postendocytic sorting of G protein-coupled receptors." Whistler J.L., Enquist J., Marley A., Fong J., Gladher F., Tsuruda P., Murray S.R., Von Zastrow M. Science 297:615-620(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH GPRASP1.
[7]	"Identification of a novel family of G protein-coupled receptor associated sorting proteins." Simonin F., Karcher P., Boeuf J.J.-M., Matifas A., Kieffer B.L. J. Neurochem. 89:766-775(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH GPRASP1.
[8]	"Variant detection at the delta opioid receptor (OPRD1) locus and population genetics of a novel variant affecting protein sequence." Gelernter J., Kranzler H.R. Hum. Genet. 107:86-88(2000) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT PHE-27.
[9]	"Opioid receptor random mutagenesis reveals a mechanism for G protein-coupled receptor activation." Decaillot F.M., Befort K., Filliol D., Yue S., Walker P., Kieffer B.L. Nat. Struct. Biol. 10:629-636(2003) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING.
+	Additional computationally mapped references.

Web resources

Wikipedia

Delta opioid receptor entry

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U07882 mRNA. Translation: AAA18789.2.
U10504 mRNA. Translation: AAA83426.1.
EU883570 mRNA. Translation: ACG60644.1.
AL009181 Genomic DNA. Translation: CAA15671.1.

IPI

IPI00298305.

PIR

I38532.

RefSeq

NP_000902.3. NM_000911.3.

UniGene

Hs.372.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1OZC	model	-	A	1-372	[»]
2IQM	model	-	A	45-336	[»]

ProteinModelPortal

P41143.

SMR

P41143. Positions 41-328.

ModBase

Search...

Protein-protein interaction databases

IntAct

P41143. 1 interaction.

STRING

9606.ENSP00000234961.

Protein family/group databases

GPCRDB

Search...

PTM databases

PhosphoSite

P41143.

Polymorphism databases

DMDM

311033488.

Proteomic databases

PaxDb

P41143.

PRIDE

P41143.

Protocols and materials databases

DNASU

4985.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000234961; ENSP00000234961; ENSG00000116329.

GeneID

4985.

KEGG

hsa:4985.

UCSC

uc001brf.1. human.

Organism-specific databases

CTD

4985.

GeneCards

GC01P029144.

HGNC

HGNC:8153. OPRD1.

HPA

HPA013744.

MIM

165195. gene.

neXtProt

NX_P41143.

PharmGKB

PA31942.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG241795.

HOGENOM

HOG000230486.

HOVERGEN

HBG106919.

InParanoid

P41143.

KO

K04213.

OMA

LCRKPCG.

OrthoDB

EOG4XWFZ5.

PhylomeDB

P41143.

Enzyme and pathway databases

Reactome

REACT_111102. Signal Transduction.

Gene expression databases

Bgee

P41143.

CleanEx

HS_OPRD1.

Genevestigator

P41143.

GermOnline

ENSG00000116329. Homo sapiens.

Family and domain databases

InterPro

IPR000321. Delta_opi_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001418. Opioid_rcpt.
[Graphical view]

Pfam

PF00001. 7tm_1. 1 hit.
[Graphical view]

PRINTS

PR00525. DELTAOPIOIDR.
PR00237. GPCRRHODOPSN.
PR00384. OPIOIDR.

PROSITE

PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

P41143.

ChEMBL

CHEMBL236.

DrugBank

DB00611. Butorphanol.
DB00318. Codeine.
DB00813. Fentanyl.
DB00956. Hydrocodone.
DB00327. Hydromorphone.
DB00836. Loperamide.
DB00295. Morphine.
DB00844. Nalbuphine.
DB01183. Naloxone.
DB00704. Naltrexone.
DB00497. Oxycodone.
DB01100. Pimozide.
DB00647. Propoxyphene.

GenomeRNAi

4985.

NextBio

19190.

SOURCE

Search...

Entry information

Entry name

OPRD_HUMAN

Accession

Primary (citable) accession number: P41143
Secondary accession number(s): B5B0B8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	November 2, 2010
Last modified:	April 3, 2013
This is version 121 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.