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Protein

Serine protease 2

Gene
N/A
Organism
Streptomyces fradiae (Streptomyces roseoflavus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Broad substrate specificity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei35 – 351Charge relay system
Active sitei65 – 651Charge relay system
Active sitei147 – 1471Charge relay system

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.431.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine protease 2 (EC:3.4.21.-)
Alternative name(s):
SFase-2
OrganismiStreptomyces fradiae (Streptomyces roseoflavus)
Taxonomic identifieri1906 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 174174Serine protease 2PRO_0000093857Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi15 ↔ 36
Disulfide bondi141 ↔ 168

Keywords - PTMi

Disulfide bond

Structurei

Secondary structure

1
174
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 94Combined sources
Beta strandi14 – 163Combined sources
Beta strandi19 – 235Combined sources
Beta strandi28 – 325Combined sources
Helixi34 – 374Combined sources
Beta strandi41 – 466Combined sources
Beta strandi51 – 6010Combined sources
Beta strandi62 – 643Combined sources
Beta strandi66 – 727Combined sources
Helixi74 – 763Combined sources
Beta strandi79 – 824Combined sources
Beta strandi84 – 863Combined sources
Beta strandi88 – 903Combined sources
Beta strandi102 – 1076Combined sources
Turni108 – 1103Combined sources
Beta strandi111 – 12515Combined sources
Beta strandi131 – 1399Combined sources
Beta strandi150 – 1534Combined sources
Beta strandi156 – 16712Combined sources
Turni168 – 1703Combined sources
Beta strandi171 – 1744Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2SFAX-ray1.60A1-174[»]
ProteinModelPortaliP41140.
SMRiP41140. Positions 1-174.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP41140.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S1 family.Curated

Family and domain databases

InterProiIPR001316. Pept_S1A_streptogrisin.
IPR009003. Peptidase_S1_PA.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00861. ALYTICPTASE.
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P41140-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
IAGGEAIYAA GGGRCSLGFN VRSSSGATYA LTAGHCTEIA STWYTNSGQT
60 70 80 90 100
SLLGTRAGTS FPGNDYGLIR HSNASAADGR VYLYNGSYRD ITGAGNAYVG
110 120 130 140 150
QTVQRSGSTT GLHSGRVTGL NATVNYGGGD IVSGLIQTNV CAEPGDSGGA
160 170
LFAGSTALGL TSGGSGNCRT GGTT
Length:174
Mass (Da):17,179
Last modified:February 1, 1995 - v1
Checksum:i240BF8B84AC572EA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S68947 Genomic DNA. Translation: AAB30080.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S68947 Genomic DNA. Translation: AAB30080.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2SFAX-ray1.60A1-174[»]
ProteinModelPortaliP41140.
SMRiP41140. Positions 1-174.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS01.431.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP41140.

Family and domain databases

InterProiIPR001316. Pept_S1A_streptogrisin.
IPR009003. Peptidase_S1_PA.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00861. ALYTICPTASE.
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Purification, characterization, primary structure, crystallization and preliminary crystallographic study of a serine proteinase from Streptomyces fradiae ATCC 14544."
    Kitadokoro K., Tsuzuki H., Nakamura E., Sato T., Teraoka H.
    Eur. J. Biochem. 220:55-61(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 14544 / DSM 40758 / IMRU 3739 / NCIMB 11726 / NRRL B-2841.
  2. "Crystal structure analysis of a serine proteinase from Streptomyces fradiae at 0.16-nm resolution and molecular modeling of an acidic-amino-acid-specific proteinase."
    Kitadokoro K., Tsuzuki H., Okamoto H., Sato T.
    Eur. J. Biochem. 224:735-742(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).
    Strain: ATCC 14544 / DSM 40758 / IMRU 3739 / NCIMB 11726 / NRRL B-2841.

Entry informationi

Entry nameiSFAS2_STRFR
AccessioniPrimary (citable) accession number: P41140
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 11, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.