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Protein

DNA-binding protein inhibitor ID-4

Gene

Id4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation.

GO - Molecular functioni

  1. RNA polymerase II transcription factor binding Source: MGI

GO - Biological processi

  1. brain development Source: MGI
  2. cell proliferation Source: MGI
  3. cellular protein localization Source: MGI
  4. central nervous system myelination Source: MGI
  5. cerebral cortex neuron differentiation Source: MGI
  6. circadian rhythm Source: UniProtKB
  7. fat cell differentiation Source: MGI
  8. G1/S transition of mitotic cell cycle Source: MGI
  9. hippocampus development Source: MGI
  10. negative regulation of astrocyte differentiation Source: MGI
  11. negative regulation of fat cell differentiation Source: MGI
  12. negative regulation of neuron differentiation Source: MGI
  13. negative regulation of oligodendrocyte differentiation Source: MGI
  14. negative regulation of transcription, DNA-templated Source: MGI
  15. negative regulation of transcription from RNA polymerase II promoter Source: MGI
  16. neuroblast proliferation Source: MGI
  17. positive regulation of cell proliferation Source: MGI
  18. positive regulation of osteoblast differentiation Source: MGI
  19. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  20. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-binding protein inhibitor ID-4
Alternative name(s):
Inhibitor of DNA binding 4
Inhibitor of differentiation 4
Gene namesi
Name:Id4
Synonyms:Id-4, Idb4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 13

Organism-specific databases

MGIiMGI:99414. Id4.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice do not exhibit a noticeable circadian phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 161161DNA-binding protein inhibitor ID-4PRO_0000127251Add
BLAST

Proteomic databases

MaxQBiP41139.
PRIDEiP41139.

Expressioni

Inductioni

Expressed in a circadian manner in the suprachiasmatic nucleus (SCN) of the brain with peak levels between CT16 and CT20.1 Publication

Gene expression databases

BgeeiP41139.
CleanExiMM_ID4.
ExpressionAtlasiP41139. baseline and differential.
GenevestigatoriP41139.

Interactioni

Subunit structurei

Heterodimer with other HLH proteins.

Binary interactionsi

WithEntry#Exp.IntActNotes
Olig1Q9JKN55EBI-1213725,EBI-1213712
Olig2Q9EQW65EBI-1213725,EBI-1213740

Protein-protein interaction databases

BioGridi200508. 2 interactions.
IntActiP41139. 2 interactions.
STRINGi10090.ENSMUSP00000021810.

Structurei

3D structure databases

ProteinModelPortaliP41139.
SMRiP41139. Positions 67-111.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini52 – 10453bHLHPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi39 – 4810Poly-Ala
Compositional biasi118 – 1247Poly-Pro

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG74134.
HOGENOMiHOG000234788.
HOVERGENiHBG009009.
InParanoidiP41139.
KOiK17695.
OMAiGACPVAP.
OrthoDBiEOG7X9G8G.
PhylomeDBiP41139.
TreeFamiTF326217.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR026052. DNA-bd_prot-inh.
[Graphical view]
PANTHERiPTHR11723. PTHR11723. 1 hit.
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P41139-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAVSPVRPS GRKAPSGCGG GELALRCLAE HGHSLGGSAA AAAAAAAARC
60 70 80 90 100
KAAEAAADEP ALCLQCDMND CYSRLRRLVP TIPPNKKVSK VEILQHVIDY
110 120 130 140 150
ILDLQLALET HPALLRQPPP PAPPLHPAGA CPVAPPRTPL TALNTDPAGA
160
VNKQGDSILC R
Length:161
Mass (Da):16,596
Last modified:February 1, 1995 - v1
Checksum:i2DCFF47AF7EE7EED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75018 mRNA. Translation: CAA52926.1.
AJ001972 Genomic DNA. Translation: CAA05120.1.
AF077859 Genomic DNA. Translation: AAD05213.1.
CCDSiCCDS26492.1.
PIRiS43260.
RefSeqiNP_112443.1. NM_031166.2.
UniGeneiMm.458006.

Genome annotation databases

EnsembliENSMUST00000021810; ENSMUSP00000021810; ENSMUSG00000021379.
GeneIDi15904.
KEGGimmu:15904.
UCSCiuc007qid.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X75018 mRNA. Translation: CAA52926.1.
AJ001972 Genomic DNA. Translation: CAA05120.1.
AF077859 Genomic DNA. Translation: AAD05213.1.
CCDSiCCDS26492.1.
PIRiS43260.
RefSeqiNP_112443.1. NM_031166.2.
UniGeneiMm.458006.

3D structure databases

ProteinModelPortaliP41139.
SMRiP41139. Positions 67-111.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200508. 2 interactions.
IntActiP41139. 2 interactions.
STRINGi10090.ENSMUSP00000021810.

Proteomic databases

MaxQBiP41139.
PRIDEiP41139.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021810; ENSMUSP00000021810; ENSMUSG00000021379.
GeneIDi15904.
KEGGimmu:15904.
UCSCiuc007qid.1. mouse.

Organism-specific databases

CTDi3400.
MGIiMGI:99414. Id4.

Phylogenomic databases

eggNOGiNOG74134.
HOGENOMiHOG000234788.
HOVERGENiHBG009009.
InParanoidiP41139.
KOiK17695.
OMAiGACPVAP.
OrthoDBiEOG7X9G8G.
PhylomeDBiP41139.
TreeFamiTF326217.

Miscellaneous databases

NextBioi288632.
PROiP41139.
SOURCEiSearch...

Gene expression databases

BgeeiP41139.
CleanExiMM_ID4.
ExpressionAtlasiP41139. baseline and differential.
GenevestigatoriP41139.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR026052. DNA-bd_prot-inh.
[Graphical view]
PANTHERiPTHR11723. PTHR11723. 1 hit.
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The expression pattern of Id4, a novel dominant negative helix-loop-helix protein, is distinct from Id1, Id2 and Id3."
    Riechmann V., van Cruechten I., Sablitzky F.
    Nucleic Acids Res. 22:749-755(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Bone marrow.
  2. "Structure, chromosomal localisation and expression of the murine dominant negative helix-loop-helix Id4 gene."
    van Cruechten I., Cinato E., Fox M., King E.R., Newton J.S., Riechmann V., Sablitzky F.
    Biochim. Biophys. Acta 1443:55-64(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The mouse Id2 and Id4 genes: structural organization and chromosomal localization."
    Mantani A., Hernandez M.-C., Kuo W.-L., Israel M.A.
    Gene 222:229-235(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "A role for Id2 in regulating photic entrainment of the mammalian circadian system."
    Duffield G.E., Watson N.P., Mantani A., Peirson S.N., Robles-Murguia M., Loros J.J., Israel M.A., Dunlap J.C.
    Curr. Biol. 19:297-304(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiID4_MOUSE
AccessioniPrimary (citable) accession number: P41139
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: February 4, 2015
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.