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Protein

DNA-binding protein inhibitor ID-3

Gene

Id3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Involved in myogenesis by inhibiting skeletal muscle and cardiac myocyte differentiation and promoting muscle precursor cells proliferation. Inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer (By similarity).By similarity

GO - Molecular functioni

  • bHLH transcription factor binding Source: RGD
  • leptomycin B binding Source: Ensembl
  • protein domain specific binding Source: RGD
  • transcription factor activity, sequence-specific DNA binding Source: Ensembl

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Biological rhythms, Myogenesis, Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-binding protein inhibitor ID-3
Alternative name(s):
Inhibitor of DNA binding 3
Inhibitor of differentiation 3
Gene namesi
Name:Id3
Synonyms:Id-3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi2860. Id3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: Ensembl
  • microtubule cytoskeleton Source: Ensembl
  • nucleoplasm Source: Ensembl
  • nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 119119DNA-binding protein inhibitor ID-3PRO_0000127249Add
BLAST

Post-translational modificationi

Phosphorylated in vitro by CDC2 and PKC.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP41138.
PRIDEiP41138.

PTM databases

PhosphoSiteiP41138.

Expressioni

Gene expression databases

GenevisibleiP41138. RN.

Interactioni

Subunit structurei

Homodimer, and heterodimer with other HLH proteins. Interacts with COPS5 and COPS7A. Interacts with IFI204. Interacts with GATA4 and NKX2-5. Interacts with ANKRD2; both proteins cooperate in myoblast differentiation. Interacts with CLOCK and ARNTL/BMAL1 (By similarity).By similarity

GO - Molecular functioni

  • bHLH transcription factor binding Source: RGD
  • protein domain specific binding Source: RGD

Protein-protein interaction databases

BioGridi247615. 2 interactions.
STRINGi10116.ENSRNOP00000035270.

Structurei

3D structure databases

ProteinModelPortaliP41138.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini28 – 8053bHLHPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IYKY. Eukaryota.
ENOG4112051. LUCA.
GeneTreeiENSGT00390000000908.
HOGENOMiHOG000234788.
HOVERGENiHBG009009.
InParanoidiP41138.
KOiK17694.
OMAiPARGCYE.
OrthoDBiEOG7X9G8G.
PhylomeDBiP41138.
TreeFamiTF326217.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR026052. DNA-bd_prot-inh.
[Graphical view]
PANTHERiPTHR11723. PTHR11723. 1 hit.
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P41138-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKALSPVRGC YEAVCCLSER SLAIARGRGK SPSAEEPLSL LDDMNHCYSR
60 70 80 90 100
LRELVPGVPR GTQLSQVEIL QRVIDYILDL QVVLAEPAPG PPDGPHLPIQ
110
TAELTPELVI SKDKRSFCH
Length:119
Mass (Da):13,059
Last modified:February 1, 1995 - v1
Checksum:iA8B6AC04D2C9A5A4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10864 mRNA. Translation: BAA01635.1.
BC064658 mRNA. Translation: AAH64658.1.
PIRiJC2113.
RefSeqiNP_037190.1. NM_013058.2.
UniGeneiRn.2760.

Genome annotation databases

EnsembliENSRNOT00000035788; ENSRNOP00000035270; ENSRNOG00000026124.
GeneIDi25585.
KEGGirno:25585.
UCSCiRGD:2860. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10864 mRNA. Translation: BAA01635.1.
BC064658 mRNA. Translation: AAH64658.1.
PIRiJC2113.
RefSeqiNP_037190.1. NM_013058.2.
UniGeneiRn.2760.

3D structure databases

ProteinModelPortaliP41138.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi247615. 2 interactions.
STRINGi10116.ENSRNOP00000035270.

PTM databases

PhosphoSiteiP41138.

Proteomic databases

PaxDbiP41138.
PRIDEiP41138.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000035788; ENSRNOP00000035270; ENSRNOG00000026124.
GeneIDi25585.
KEGGirno:25585.
UCSCiRGD:2860. rat.

Organism-specific databases

CTDi3399.
RGDi2860. Id3.

Phylogenomic databases

eggNOGiENOG410IYKY. Eukaryota.
ENOG4112051. LUCA.
GeneTreeiENSGT00390000000908.
HOGENOMiHOG000234788.
HOVERGENiHBG009009.
InParanoidiP41138.
KOiK17694.
OMAiPARGCYE.
OrthoDBiEOG7X9G8G.
PhylomeDBiP41138.
TreeFamiTF326217.

Miscellaneous databases

NextBioi607247.
PROiP41138.

Gene expression databases

GenevisibleiP41138. RN.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR026052. DNA-bd_prot-inh.
[Graphical view]
PANTHERiPTHR11723. PTHR11723. 1 hit.
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Activation of helix-loop-helix proteins Id1, Id2 and Id3 during neural differentiation."
    Nagata Y., Todokoro K.
    Biochem. Biophys. Res. Commun. 199:1355-1362(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Prostate.
  3. "Phosphorylation of helix-loop-helix proteins ID1, ID2 and ID3."
    Nagata Y., Shoji W., Obinata M., Todokoro K.
    Biochem. Biophys. Res. Commun. 207:916-926(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION.

Entry informationi

Entry nameiID3_RAT
AccessioniPrimary (citable) accession number: P41138
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 11, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.