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Protein

DNA-binding protein inhibitor ID-1

Gene

Id1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer (By similarity).By similarity

GO - Molecular functioni

  • proteasome binding Source: RGD
  • protein C-terminus binding Source: RGD
  • protein homodimerization activity Source: RGD
  • protein N-terminus binding Source: RGD
  • protein self-association Source: Ensembl
  • transcription factor activity, sequence-specific DNA binding Source: Ensembl
  • transcription factor binding Source: RGD

GO - Biological processi

  • BMP signaling pathway Source: Ensembl
  • brain development Source: RGD
  • cell-abiotic substrate adhesion Source: RGD
  • cellular response to dopamine Source: RGD
  • cellular response to epidermal growth factor stimulus Source: RGD
  • cellular response to nerve growth factor stimulus Source: RGD
  • cellular response to peptide Source: RGD
  • cellular response to transforming growth factor beta stimulus Source: RGD
  • circadian rhythm Source: Ensembl
  • collagen metabolic process Source: Ensembl
  • endothelial cell morphogenesis Source: Ensembl
  • heart development Source: Ensembl
  • lung morphogenesis Source: Ensembl
  • lung vasculature development Source: Ensembl
  • negative regulation of apoptotic process Source: RGD
  • negative regulation of dendrite morphogenesis Source: RGD
  • negative regulation of endothelial cell differentiation Source: RGD
  • negative regulation of osteoblast differentiation Source: Ensembl
  • negative regulation of protein binding Source: RGD
  • negative regulation of protein homodimerization activity Source: Ensembl
  • negative regulation of RNA polymerase II regulatory region sequence-specific DNA binding Source: RGD
  • negative regulation of sequence-specific DNA binding transcription factor activity Source: Ensembl
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter Source: RGD
  • neuron differentiation Source: RGD
  • positive regulation of actin filament bundle assembly Source: RGD
  • positive regulation of epithelial cell proliferation Source: RGD
  • positive regulation of gene expression Source: RGD
  • protein destabilization Source: Ensembl
  • regulation of angiogenesis Source: Ensembl
  • regulation of MAPK cascade Source: Ensembl
  • regulation of vasculature development Source: RGD
  • response to antibiotic Source: Ensembl
  • response to organic cyclic compound Source: RGD
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionDevelopmental protein, Repressor
Biological processBiological rhythms, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-RNO-2559585. Oncogene Induced Senescence.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-binding protein inhibitor ID-1
Alternative name(s):
Inhibitor of DNA binding 1
Inhibitor of differentiation 1
Gene namesi
Name:Id1
Synonyms:Id-1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi2858. Id1.

Subcellular locationi

GO - Cellular componenti

  • centrosome Source: RGD
  • Golgi apparatus Source: Ensembl
  • nucleoplasm Source: Ensembl
  • nucleus Source: RGD

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001272381 – 164DNA-binding protein inhibitor ID-1Add BLAST164

Post-translational modificationi

Phosphorylated in vitro by PKA and PKC.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP41135.
PRIDEiP41135.

Expressioni

Gene expression databases

BgeeiENSRNOG00000021750.
ExpressionAtlasiP41135. baseline and differential.
GenevisibleiP41135. RN.

Interactioni

Subunit structurei

Heterodimer with other HLH proteins. Interacts with COPS5, IFI204, GATA4, NKX2-5, CLOCK and ARNTL/BMAL1 (By similarity). Isoform Short can form homodimers.By similarity

GO - Molecular functioni

  • proteasome binding Source: RGD
  • protein C-terminus binding Source: RGD
  • protein homodimerization activity Source: RGD
  • protein N-terminus binding Source: RGD
  • protein self-association Source: Ensembl
  • transcription factor binding Source: RGD

Protein-protein interaction databases

BioGridi247301. 2 interactors.
IntActiP41135. 2 interactors.
STRINGi10116.ENSRNOP00000052261.

Structurei

3D structure databases

ProteinModelPortaliP41135.
SMRiP41135.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini46 – 98bHLHPROSITE-ProRule annotationAdd BLAST53

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi91 – 104Nuclear export signalBy similarityAdd BLAST14

Phylogenomic databases

eggNOGiENOG410IWDR. Eukaryota.
ENOG4111VAY. LUCA.
GeneTreeiENSGT00390000000908.
HOGENOMiHOG000234788.
HOVERGENiHBG009009.
InParanoidiP41135.
KOiK04680.
OMAiYIWDLEL.
OrthoDBiEOG091G0VHR.
PhylomeDBiP41135.
TreeFamiTF326217.

Family and domain databases

CDDicd00083. HLH. 1 hit.
Gene3Di4.10.280.10. 1 hit.
InterProiView protein in InterPro
IPR011598. bHLH_dom.
IPR026052. DNA-bd_prot-inh.
PANTHERiPTHR11723. PTHR11723. 1 hit.
PfamiView protein in Pfam
PF00010. HLH. 1 hit.
SMARTiView protein in SMART
SM00353. HLH. 1 hit.
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiView protein in PROSITE
PS50888. BHLH. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform Long (identifier: P41135-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKVASSSAAA TAGPSCSLKA GRTAGEVVLG LSEQSVAISR CAGTRLPALL
60 70 80 90 100
DEQQVNVLLY DMNGCYSRLK ELVPTLPQNR KVSKVEILQH VIDYIRDLQL
110 120 130 140 150
ELNSESEVAT AGGRGLPVRA PLSTLNGEIS ALAAEVRSES EYYIILLWET
160
KATGGGCPPY FSGA
Length:164
Mass (Da):17,397
Last modified:February 1, 1995 - v1
Checksum:iC782DDF4C4892D6D
GO
Isoform Short (identifier: P41135-2) [UniParc]FASTAAdd to basket
Also known as: ID1.25

The sequence of this isoform differs from the canonical sequence as follows:
     136-164: VRSESEYYIILLWETKATGGGCPPYFSGA → AACVPADDRILCR

Show »
Length:148
Mass (Da):15,604
Checksum:i98D2128DB8A69EE2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti113G → A in BAA01633 (PubMed:7908517).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_002110136 – 164VRSES…YFSGA → AACVPADDRILCR in isoform Short. CuratedAdd BLAST29

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L23148 mRNA. Translation: AAA20403.1.
D10862 mRNA. Translation: BAA01633.1.
M86708 mRNA. Translation: AAA41090.1.
PIRiA53334.
JC2111.
RefSeqiNP_036929.2. NM_012797.2. [P41135-2]
UniGeneiRn.2113.

Genome annotation databases

EnsembliENSRNOT00000029660; ENSRNOP00000052261; ENSRNOG00000021750. [P41135-2]
GeneIDi25261.
KEGGirno:25261.
UCSCiRGD:2858. rat. [P41135-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiID1_RAT
AccessioniPrimary (citable) accession number: P41135
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: May 10, 2017
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome