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Protein

DNA-binding protein inhibitor ID-1

Gene

Id1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer (By similarity).By similarity

GO - Molecular functioni

  • proteasome binding Source: RGD
  • protein C-terminus binding Source: RGD
  • protein homodimerization activity Source: RGD
  • protein N-terminus binding Source: RGD
  • transcription factor activity, sequence-specific DNA binding Source: Ensembl
  • transcription factor binding Source: RGD

GO - Biological processi

  • BMP signaling pathway Source: Ensembl
  • brain development Source: RGD
  • cell-abiotic substrate adhesion Source: RGD
  • cellular response to dopamine Source: RGD
  • cellular response to epidermal growth factor stimulus Source: RGD
  • cellular response to nerve growth factor stimulus Source: RGD
  • cellular response to peptide Source: RGD
  • cellular response to transforming growth factor beta stimulus Source: RGD
  • circadian rhythm Source: Ensembl
  • collagen metabolic process Source: Ensembl
  • endothelial cell morphogenesis Source: Ensembl
  • heart development Source: Ensembl
  • lung morphogenesis Source: Ensembl
  • lung vasculature development Source: Ensembl
  • negative regulation of apoptotic process Source: RGD
  • negative regulation of dendrite morphogenesis Source: RGD
  • negative regulation of endothelial cell differentiation Source: RGD
  • negative regulation of osteoblast differentiation Source: Ensembl
  • negative regulation of protein binding Source: RGD
  • negative regulation of protein homodimerization activity Source: Ensembl
  • negative regulation of RNA polymerase II regulatory region sequence-specific DNA binding Source: RGD
  • negative regulation of sequence-specific DNA binding transcription factor activity Source: Ensembl
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter Source: RGD
  • neuron differentiation Source: RGD
  • positive regulation of actin filament bundle assembly Source: RGD
  • positive regulation of epithelial cell proliferation Source: RGD
  • positive regulation of gene expression Source: RGD
  • protein destabilization Source: Ensembl
  • regulation of angiogenesis Source: Ensembl
  • regulation of MAPK cascade Source: Ensembl
  • regulation of vasculature development Source: RGD
  • response to antibiotic Source: Ensembl
  • response to organic cyclic compound Source: RGD
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Repressor

Keywords - Biological processi

Biological rhythms, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-RNO-2559585. Oncogene Induced Senescence.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-binding protein inhibitor ID-1
Alternative name(s):
Inhibitor of DNA binding 1
Inhibitor of differentiation 1
Gene namesi
Name:Id1
Synonyms:Id-1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi2858. Id1.

Subcellular locationi

GO - Cellular componenti

  • centrosome Source: RGD
  • Golgi apparatus Source: Ensembl
  • nucleoplasm Source: Ensembl
  • nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 164164DNA-binding protein inhibitor ID-1PRO_0000127238Add
BLAST

Post-translational modificationi

Phosphorylated in vitro by PKA and PKC.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP41135.
PRIDEiP41135.

Expressioni

Gene expression databases

ExpressionAtlasiP41135. baseline and differential.
GenevisibleiP41135. RN.

Interactioni

Subunit structurei

Heterodimer with other HLH proteins. Interacts with COPS5, IFI204, GATA4, NKX2-5, CLOCK and ARNTL/BMAL1 (By similarity). Isoform Short can form homodimers.By similarity

GO - Molecular functioni

  • proteasome binding Source: RGD
  • protein C-terminus binding Source: RGD
  • protein homodimerization activity Source: RGD
  • protein N-terminus binding Source: RGD
  • transcription factor binding Source: RGD

Protein-protein interaction databases

BioGridi247301. 2 interactions.
IntActiP41135. 2 interactions.
STRINGi10116.ENSRNOP00000052261.

Structurei

3D structure databases

ProteinModelPortaliP41135.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini46 – 9853bHLHPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi91 – 10414Nuclear export signalBy similarityAdd
BLAST

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IWDR. Eukaryota.
ENOG4111VAY. LUCA.
GeneTreeiENSGT00390000000908.
HOGENOMiHOG000234788.
HOVERGENiHBG009009.
InParanoidiP41135.
KOiK04680.
OMAiYIWDLEL.
OrthoDBiEOG7X9G8G.
PhylomeDBiP41135.
TreeFamiTF326217.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR026052. DNA-bd_prot-inh.
[Graphical view]
PANTHERiPTHR11723. PTHR11723. 1 hit.
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.

Isoform Long (identifier: P41135-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKVASSSAAA TAGPSCSLKA GRTAGEVVLG LSEQSVAISR CAGTRLPALL
60 70 80 90 100
DEQQVNVLLY DMNGCYSRLK ELVPTLPQNR KVSKVEILQH VIDYIRDLQL
110 120 130 140 150
ELNSESEVAT AGGRGLPVRA PLSTLNGEIS ALAAEVRSES EYYIILLWET
160
KATGGGCPPY FSGA
Length:164
Mass (Da):17,397
Last modified:February 1, 1995 - v1
Checksum:iC782DDF4C4892D6D
GO
Isoform Short (identifier: P41135-2) [UniParc]FASTAAdd to basket

Also known as: ID1.25

The sequence of this isoform differs from the canonical sequence as follows:
     136-164: VRSESEYYIILLWETKATGGGCPPYFSGA → AACVPADDRILCR

Show »
Length:148
Mass (Da):15,604
Checksum:i98D2128DB8A69EE2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti113 – 1131G → A in BAA01633 (PubMed:7908517).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei136 – 16429VRSES…YFSGA → AACVPADDRILCR in isoform Short. CuratedVSP_002110Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L23148 mRNA. Translation: AAA20403.1.
D10862 mRNA. Translation: BAA01633.1.
M86708 mRNA. Translation: AAA41090.1.
PIRiA53334.
JC2111.
RefSeqiNP_036929.2. NM_012797.2. [P41135-2]
UniGeneiRn.2113.

Genome annotation databases

EnsembliENSRNOT00000029660; ENSRNOP00000052261; ENSRNOG00000021750. [P41135-2]
GeneIDi25261.
KEGGirno:25261.
UCSCiRGD:2858. rat. [P41135-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L23148 mRNA. Translation: AAA20403.1.
D10862 mRNA. Translation: BAA01633.1.
M86708 mRNA. Translation: AAA41090.1.
PIRiA53334.
JC2111.
RefSeqiNP_036929.2. NM_012797.2. [P41135-2]
UniGeneiRn.2113.

3D structure databases

ProteinModelPortaliP41135.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi247301. 2 interactions.
IntActiP41135. 2 interactions.
STRINGi10116.ENSRNOP00000052261.

Proteomic databases

PaxDbiP41135.
PRIDEiP41135.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000029660; ENSRNOP00000052261; ENSRNOG00000021750. [P41135-2]
GeneIDi25261.
KEGGirno:25261.
UCSCiRGD:2858. rat. [P41135-1]

Organism-specific databases

CTDi3397.
RGDi2858. Id1.

Phylogenomic databases

eggNOGiENOG410IWDR. Eukaryota.
ENOG4111VAY. LUCA.
GeneTreeiENSGT00390000000908.
HOGENOMiHOG000234788.
HOVERGENiHBG009009.
InParanoidiP41135.
KOiK04680.
OMAiYIWDLEL.
OrthoDBiEOG7X9G8G.
PhylomeDBiP41135.
TreeFamiTF326217.

Enzyme and pathway databases

ReactomeiR-RNO-2559585. Oncogene Induced Senescence.

Miscellaneous databases

PROiP41135.

Gene expression databases

ExpressionAtlasiP41135. baseline and differential.
GenevisibleiP41135. RN.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
IPR026052. DNA-bd_prot-inh.
[Graphical view]
PANTHERiPTHR11723. PTHR11723. 1 hit.
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Posttranscriptional regulation of Id1 activity in cardiac muscle. Alternative splicing of novel Id1 transcript permits homodimerization."
    Springhorn J.P., Singh K., Kelly R.A., Smith T.W.
    J. Biol. Chem. 269:5132-5136(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Heart.
  2. "Transcriptional regulation in cardiac muscle. Coordinate expression of Id with a neonatal phenotype during development and following a hypertrophic stimulus in adult rat ventricular myocytes in vitro."
    Springhorn J.P., Ellingsen O., Berger H.J., Kelly R.A., Smith T.W.
    J. Biol. Chem. 267:14360-14365(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Heart.
  3. "Activation of helix-loop-helix proteins Id1, Id2 and Id3 during neural differentiation."
    Nagata Y., Todokoro K.
    Biochem. Biophys. Res. Commun. 199:1355-1362(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "Phosphorylation of helix-loop-helix proteins ID1, ID2 and ID3."
    Nagata Y., Shoji W., Obinata M., Todokoro K.
    Biochem. Biophys. Res. Commun. 207:916-926(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION.

Entry informationi

Entry nameiID1_RAT
AccessioniPrimary (citable) accession number: P41135
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: June 8, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.