P41133 (ID3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-binding protein inhibitor ID-3

Alternative name(s):
ID-like protein inhibitor HLH 462
Inhibitor of DNA binding 3

Gene names

Name:	Id3
Synonyms:	Hlh462, Id-3, Idb3

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	119 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. Involved in myogenesis by inhibiting skeletal muscle and cardiac myocyte differentiation and promoting muscle precursor cells proliferation. Inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer. May inhibit other transcription factors. Ref.5
Subunit structure	Homodimer, and heterodimer with other HLH proteins. Interacts with COPS5 and COPS7A. Interacts with IFI204. Interacts with GATA4 and NKX2-5. Ref.2 Ref.3 Ref.4
Subcellular location	Nucleus. Cytoplasm. Note: Nuclear in proliferating cells, translocates to cytosol during cell differentiation. Ref.4
Induction	By a variety of mitogenic agents in serum starved cells.
Post-translational modification	Polyubiquitinated; which is favored by Ifi204 and leads to proteasomal degradation.
Sequence similarities	Contains 1 bHLH (basic helix-loop-helix) domain.

Ontologies

Keywords
Biological process	Myogenesis
Cellular component	Cytoplasm Nucleus
Molecular function	Developmental protein
PTM	Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	central nervous system development Inferred from expression pattern PubMed 7655079. Source: UniProtKB epithelial cell differentiation Inferred from direct assay PubMed 16407553. Source: MGI heart development Inferred from genetic interaction PubMed 15472070. Source: MGI metanephros development Inferred from expression pattern PubMed 7655079. Source: UniProtKB negative regulation of osteoblast differentiation Inferred from genetic interaction PubMed 15161906. Source: MGI negative regulation of sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: Compara negative regulation of transcription from RNA polymerase II promoter Inferred from direct assay Ref.1. Source: MGI neuron differentiation Inferred from electronic annotation. Source: Compara notochord development Inferred from expression pattern PubMed 7655079. Source: UniProtKB odontogenesis Inferred from expression pattern PubMed 7655079. Source: UniProtKB positive regulation of apoptotic process Inferred from electronic annotation. Source: Compara regulation of DNA replication Inferred from electronic annotation. Source: Compara regulation of cell cycle Inferred from electronic annotation. Source: Compara response to wounding Inferred from electronic annotation. Source: Compara transcription, DNA-dependent Inferred from direct assay PubMed 17681138. Source: UniProtKB
Cellular_component	cytoplasm Inferred from direct assay PubMed 9418957. Source: MGI nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protein domain specific binding Inferred from direct assay Ref.1. Source: MGI sequence-specific DNA binding transcription factor activity Inferred from direct assay PubMed 17681138. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 119	119	DNA-binding protein inhibitor ID-3		PRO_0000127248
Regions
	Domain	28 – 80	53	bHLH
	Region	35 – 87	53	Interaction with IFI204 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P41133 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 49F3B841D2D2BF15	FASTA	119	13,089
	10 20 30 40 50 60 MKALSPVRGC YEAVCCLSER SLAIARGRGK SPSTEEPLSL LDDMNHCYSR LRELVPGVPR 70 80 90 100 110 GTQLSQVEIL QRVIDYILDL QVVLAEPAPG PPDGPHLPIQ TAELTPELVI SKDKRSFCH

References

[1]	"An Id-related helix-loop-helix protein encoded by a growth factor-inducible gene." Christy B.A., Sanders L.K., Lau L.F., Copeland N.G., Jenkins N.A., Nathans D. Proc. Natl. Acad. Sci. U.S.A. 88:1815-1819(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The MyoD-inducible p204 protein overcomes the inhibition of myoblast differentiation by Id proteins." Liu C.-J., Ding B., Wang H., Lengyel P. Mol. Cell. Biol. 22:2893-2905(2002) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH IFI204.
[3]	"Ubiquitin-dependent degradation of Id1 and Id3 is mediated by the COP9 signalosome." Berse M., Bounpheng M., Huang X., Christy B., Pollmann C., Dubiel W. J. Mol. Biol. 343:361-370(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH COPS5 AND COPS7A.
[4]	"p204 protein overcomes the inhibition of the differentiation of P19 murine embryonal carcinoma cells to beating cardiac myocytes by Id proteins." Ding B., Liu C.-J., Huang Y., Yu J., Kong W., Lengyel P. J. Biol. Chem. 281:14893-14906(2006) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH GATA4 AND NKX2-5, UBIQUITINATION, SUBCELLULAR LOCATION.
[5]	"Pax3/7BP is a Pax7- and Pax3-binding protein that regulates the proliferation of muscle precursor cells by an epigenetic mechanism." Diao Y., Guo X., Li Y., Sun K., Lu L., Jiang L., Fu X., Zhu H., Sun H., Wang H., Wu Z. Cell Stem Cell 11:231-241(2012) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M60523 mRNA. Translation: AAA37818.1.
IPI	IPI00111445.
PIR	A39114.
RefSeq	NP_032347.1. NM_008321.2.
UniGene	Mm.110.
3D structure databases
ProteinModelPortal	P41133.
SMR	P41133. Positions 32-83.
ModBase	Search...
Protein-protein interaction databases
IntAct	P41133. 3 interactions.
STRING	10090.ENSMUSP00000008016.
PTM databases
PhosphoSite	P41133.
Proteomic databases
PRIDE	P41133.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000008016; ENSMUSP00000008016; ENSMUSG00000007872.
GeneID	15903.
KEGG	mmu:15903.
Organism-specific databases
CTD	3399.
MGI	MGI:96398. Id3.
Phylogenomic databases
eggNOG	NOG83634.
HOGENOM	HOG000234788.
HOVERGEN	HBG009009.
InParanoid	P41133.
KO	K04680.
OMA	GPHLAIQ.
OrthoDB	EOG40P48F.
Gene expression databases
ArrayExpress	P41133.
Bgee	P41133.
CleanEx	MM_ID3.
Genevestigator	P41133.
GermOnline	ENSMUSG00000007872. Mus musculus.
Family and domain databases
Gene3D	4.10.280.10. 1 hit.
InterPro	IPR011598. bHLH_dom. IPR026052. DNA-bd_prot-inh. [Graphical view]
PANTHER	PTHR11723. PTHR11723. 1 hit.
Pfam	PF00010. HLH. 1 hit. [Graphical view]
SMART	SM00353. HLH. 1 hit. [Graphical view]
SUPFAM	SSF47459. HLH_basic. 1 hit.
PROSITE	PS50888. BHLH. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	288628.
SOURCE	Search...

Entry information

Entry name

ID3_MOUSE

Accession

Primary (citable) accession number: P41133

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	May 1, 2013
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program