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P41133

- ID3_MOUSE

UniProt

P41133 - ID3_MOUSE

Protein

DNA-binding protein inhibitor ID-3

Gene

Id3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Involved in myogenesis by inhibiting skeletal muscle and cardiac myocyte differentiation and promoting muscle precursor cells proliferation. Inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer.3 Publications

    GO - Molecular functioni

    1. protein binding Source: UniProtKB
    2. protein domain specific binding Source: MGI
    3. sequence-specific DNA binding transcription factor activity Source: UniProtKB
    4. transcription factor binding Source: UniProtKB

    GO - Biological processi

    1. central nervous system development Source: UniProtKB
    2. circadian rhythm Source: UniProtKB
    3. epithelial cell differentiation Source: MGI
    4. heart development Source: MGI
    5. metanephros development Source: UniProtKB
    6. muscle organ development Source: UniProtKB-KW
    7. negative regulation of myoblast differentiation Source: UniProtKB
    8. negative regulation of osteoblast differentiation Source: MGI
    9. negative regulation of sequence-specific DNA binding transcription factor activity Source: Ensembl
    10. negative regulation of transcription, DNA-templated Source: UniProtKB
    11. negative regulation of transcription from RNA polymerase II promoter Source: MGI
    12. neuron differentiation Source: Ensembl
    13. notochord development Source: UniProtKB
    14. odontogenesis Source: UniProtKB
    15. positive regulation of apoptotic process Source: Ensembl
    16. regulation of cell cycle Source: Ensembl
    17. regulation of DNA replication Source: Ensembl
    18. response to wounding Source: Ensembl
    19. transcription, DNA-templated Source: UniProtKB

    Keywords - Molecular functioni

    Developmental protein, Repressor

    Keywords - Biological processi

    Biological rhythms, Myogenesis, Transcription, Transcription regulation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA-binding protein inhibitor ID-3
    Alternative name(s):
    ID-like protein inhibitor HLH 462
    Inhibitor of DNA binding 3
    Inhibitor of differentiation 3
    Gene namesi
    Name:Id3
    Synonyms:Hlh462, Id-3, Idb3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:96398. Id3.

    Subcellular locationi

    Nucleus 1 PublicationPROSITE-ProRule annotation. Cytoplasm 1 Publication
    Note: Nuclear in proliferating cells, translocates to cytosol during cell differentiation.

    GO - Cellular componenti

    1. cytoplasm Source: MGI
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 119119DNA-binding protein inhibitor ID-3PRO_0000127248Add
    BLAST

    Post-translational modificationi

    Polyubiquitinated; which is favored by Ifi204 and leads to proteasomal degradation.1 Publication

    Keywords - PTMi

    Ubl conjugation

    Proteomic databases

    PRIDEiP41133.

    PTM databases

    PhosphoSiteiP41133.

    Expressioni

    Tissue specificityi

    Expressed by myoblasts (at protein level).1 Publication

    Inductioni

    By a variety of mitogenic agents in serum starved cells. Expressed in a circadian manner in the suprachiasmatic nucleus (SCN) of the brain and heart with peak levels between CT16 and CT20 in SCN and between CT8 and CT12 in the heart.1 Publication

    Gene expression databases

    ArrayExpressiP41133.
    BgeeiP41133.
    CleanExiMM_ID3.
    GenevestigatoriP41133.

    Interactioni

    Subunit structurei

    Homodimer, and heterodimer with other HLH proteins. Interacts with CLOCK and ARNTL/BMAL1 By similarity. Interacts with COPS5 and COPS7A. Interacts with IFI204. Interacts with GATA4 and NKX2-5. Interacts with ANKRD2; both proteins cooperate in myoblast differentiation.By similarity4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Ankrd2Q9WV064EBI-309448,EBI-8854438

    Protein-protein interaction databases

    BioGridi200507. 10 interactions.
    IntActiP41133. 4 interactions.
    STRINGi10090.ENSMUSP00000008016.

    Structurei

    3D structure databases

    ProteinModelPortaliP41133.
    SMRiP41133. Positions 32-83.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini28 – 8053bHLHPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni35 – 8753Interaction with IFI204By similarityAdd
    BLAST

    Sequence similaritiesi

    Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG83634.
    HOGENOMiHOG000234788.
    HOVERGENiHBG009009.
    InParanoidiP41133.
    KOiK17694.
    OMAiVISKDKR.
    OrthoDBiEOG7X9G8G.
    PhylomeDBiP41133.
    TreeFamiTF326217.

    Family and domain databases

    Gene3Di4.10.280.10. 1 hit.
    InterProiIPR011598. bHLH_dom.
    IPR026052. DNA-bd_prot-inh.
    [Graphical view]
    PANTHERiPTHR11723. PTHR11723. 1 hit.
    PfamiPF00010. HLH. 1 hit.
    [Graphical view]
    SMARTiSM00353. HLH. 1 hit.
    [Graphical view]
    SUPFAMiSSF47459. SSF47459. 1 hit.
    PROSITEiPS50888. BHLH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P41133-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKALSPVRGC YEAVCCLSER SLAIARGRGK SPSTEEPLSL LDDMNHCYSR    50
    LRELVPGVPR GTQLSQVEIL QRVIDYILDL QVVLAEPAPG PPDGPHLPIQ 100
    TAELTPELVI SKDKRSFCH 119
    Length:119
    Mass (Da):13,089
    Last modified:February 1, 1995 - v1
    Checksum:i49F3B841D2D2BF15
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M60523 mRNA. Translation: AAA37818.1.
    CCDSiCCDS18800.1.
    PIRiA39114.
    RefSeqiNP_032347.1. NM_008321.2.
    UniGeneiMm.110.

    Genome annotation databases

    EnsembliENSMUST00000008016; ENSMUSP00000008016; ENSMUSG00000007872.
    GeneIDi15903.
    KEGGimmu:15903.
    UCSCiuc012dnf.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M60523 mRNA. Translation: AAA37818.1 .
    CCDSi CCDS18800.1.
    PIRi A39114.
    RefSeqi NP_032347.1. NM_008321.2.
    UniGenei Mm.110.

    3D structure databases

    ProteinModelPortali P41133.
    SMRi P41133. Positions 32-83.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 200507. 10 interactions.
    IntActi P41133. 4 interactions.
    STRINGi 10090.ENSMUSP00000008016.

    PTM databases

    PhosphoSitei P41133.

    Proteomic databases

    PRIDEi P41133.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000008016 ; ENSMUSP00000008016 ; ENSMUSG00000007872 .
    GeneIDi 15903.
    KEGGi mmu:15903.
    UCSCi uc012dnf.1. mouse.

    Organism-specific databases

    CTDi 3399.
    MGIi MGI:96398. Id3.

    Phylogenomic databases

    eggNOGi NOG83634.
    HOGENOMi HOG000234788.
    HOVERGENi HBG009009.
    InParanoidi P41133.
    KOi K17694.
    OMAi VISKDKR.
    OrthoDBi EOG7X9G8G.
    PhylomeDBi P41133.
    TreeFami TF326217.

    Miscellaneous databases

    NextBioi 288628.
    PROi P41133.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P41133.
    Bgeei P41133.
    CleanExi MM_ID3.
    Genevestigatori P41133.

    Family and domain databases

    Gene3Di 4.10.280.10. 1 hit.
    InterProi IPR011598. bHLH_dom.
    IPR026052. DNA-bd_prot-inh.
    [Graphical view ]
    PANTHERi PTHR11723. PTHR11723. 1 hit.
    Pfami PF00010. HLH. 1 hit.
    [Graphical view ]
    SMARTi SM00353. HLH. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47459. SSF47459. 1 hit.
    PROSITEi PS50888. BHLH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "An Id-related helix-loop-helix protein encoded by a growth factor-inducible gene."
      Christy B.A., Sanders L.K., Lau L.F., Copeland N.G., Jenkins N.A., Nathans D.
      Proc. Natl. Acad. Sci. U.S.A. 88:1815-1819(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The MyoD-inducible p204 protein overcomes the inhibition of myoblast differentiation by Id proteins."
      Liu C.-J., Ding B., Wang H., Lengyel P.
      Mol. Cell. Biol. 22:2893-2905(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH IFI204.
    3. "Ubiquitin-dependent degradation of Id1 and Id3 is mediated by the COP9 signalosome."
      Berse M., Bounpheng M., Huang X., Christy B., Pollmann C., Dubiel W.
      J. Mol. Biol. 343:361-370(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH COPS5 AND COPS7A.
    4. "p204 protein overcomes the inhibition of the differentiation of P19 murine embryonal carcinoma cells to beating cardiac myocytes by Id proteins."
      Ding B., Liu C.-J., Huang Y., Yu J., Kong W., Lengyel P.
      J. Biol. Chem. 281:14893-14906(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH GATA4 AND NKX2-5, UBIQUITINATION, SUBCELLULAR LOCATION.
    5. "A role for Id2 in regulating photic entrainment of the mammalian circadian system."
      Duffield G.E., Watson N.P., Mantani A., Peirson S.N., Robles-Murguia M., Loros J.J., Israel M.A., Dunlap J.C.
      Curr. Biol. 19:297-304(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION.
    6. "Pax3/7BP is a Pax7- and Pax3-binding protein that regulates the proliferation of muscle precursor cells by an epigenetic mechanism."
      Diao Y., Guo X., Li Y., Sun K., Lu L., Jiang L., Fu X., Zhu H., Sun H., Wang H., Wu Z.
      Cell Stem Cell 11:231-241(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Ankyrin repeat domain protein 2 and inhibitor of DNA binding 3 cooperatively inhibit myoblast differentiation by physical interaction."
      Mohamed J.S., Lopez M.A., Cox G.A., Boriek A.M.
      J. Biol. Chem. 288:24560-24568(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN MYOBLAST DIFFERENTIATION, INTERACTION WITH ANKRD2, TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiID3_MOUSE
    AccessioniPrimary (citable) accession number: P41133
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: February 1, 1995
    Last modified: October 1, 2014
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3