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P41133 (ID3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA-binding protein inhibitor ID-3
Alternative name(s):
ID-like protein inhibitor HLH 462
Inhibitor of DNA binding 3
Gene names
Name:Id3
Synonyms:Hlh462, Id-3, Idb3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length119 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. Involved in myogenesis by inhibiting skeletal muscle and cardiac myocyte differentiation and promoting muscle precursor cells proliferation. Inhibits the binding of E2A-containing protein complexes to muscle creatine kinase E-box enhancer. May inhibit other transcription factors. Ref.5 Ref.6

Subunit structure

Homodimer, and heterodimer with other HLH proteins. Interacts with COPS5 and COPS7A. Interacts with IFI204. Interacts with GATA4 and NKX2-5. Interacts with ANKRD2; both proteins cooperate in myoblast differentiation. Ref.2 Ref.3 Ref.4 Ref.6

Subcellular location

Nucleus. Cytoplasm. Note: Nuclear in proliferating cells, translocates to cytosol during cell differentiation. Ref.4

Tissue specificity

Expressed by myoblasts (at protein level). Ref.6

Induction

By a variety of mitogenic agents in serum starved cells.

Post-translational modification

Polyubiquitinated; which is favored by Ifi204 and leads to proteasomal degradation.

Sequence similarities

Contains 1 bHLH (basic helix-loop-helix) domain.

Ontologies

Keywords
   Biological processMyogenesis
   Cellular componentCytoplasm
Nucleus
   Molecular functionDevelopmental protein
   PTMUbl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcentral nervous system development

Inferred from expression pattern PubMed 7655079. Source: UniProtKB

epithelial cell differentiation

Inferred from direct assay PubMed 16407553. Source: MGI

heart development

Inferred from genetic interaction PubMed 15472070. Source: MGI

metanephros development

Inferred from expression pattern PubMed 7655079. Source: UniProtKB

muscle organ development

Inferred from electronic annotation. Source: UniProtKB-KW

negative regulation of myoblast differentiation

Inferred from mutant phenotype Ref.6. Source: UniProtKB

negative regulation of osteoblast differentiation

Inferred from genetic interaction PubMed 15161906. Source: MGI

negative regulation of sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: Ensembl

negative regulation of transcription from RNA polymerase II promoter

Inferred from direct assay Ref.1. Source: MGI

neuron differentiation

Inferred from electronic annotation. Source: Ensembl

notochord development

Inferred from expression pattern PubMed 7655079. Source: UniProtKB

odontogenesis

Inferred from expression pattern PubMed 7655079. Source: UniProtKB

positive regulation of apoptotic process

Inferred from electronic annotation. Source: Ensembl

regulation of DNA replication

Inferred from electronic annotation. Source: Ensembl

regulation of cell cycle

Inferred from electronic annotation. Source: Ensembl

response to wounding

Inferred from electronic annotation. Source: Ensembl

transcription, DNA-templated

Inferred from direct assay PubMed 17681138. Source: UniProtKB

   Cellular_componentcytoplasm

Inferred from direct assay PubMed 9418957. Source: MGI

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionprotein binding

Inferred from physical interaction PubMed 17681138PubMed 8759016. Source: UniProtKB

protein domain specific binding

Inferred from direct assay Ref.1. Source: MGI

sequence-specific DNA binding transcription factor activity

Inferred from direct assay PubMed 17681138. Source: UniProtKB

transcription factor binding

Inferred from physical interaction PubMed 8759016. Source: UniProtKB

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Ankrd2Q9WV064EBI-309448,EBI-8854438

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 119119DNA-binding protein inhibitor ID-3
PRO_0000127248

Regions

Domain28 – 8053bHLH
Region35 – 8753Interaction with IFI204 By similarity

Sequences

Sequence LengthMass (Da)Tools
P41133 [UniParc].

Last modified February 1, 1995. Version 1.
Checksum: 49F3B841D2D2BF15

FASTA11913,089
        10         20         30         40         50         60 
MKALSPVRGC YEAVCCLSER SLAIARGRGK SPSTEEPLSL LDDMNHCYSR LRELVPGVPR 

        70         80         90        100        110 
GTQLSQVEIL QRVIDYILDL QVVLAEPAPG PPDGPHLPIQ TAELTPELVI SKDKRSFCH 

« Hide

References

[1]"An Id-related helix-loop-helix protein encoded by a growth factor-inducible gene."
Christy B.A., Sanders L.K., Lau L.F., Copeland N.G., Jenkins N.A., Nathans D.
Proc. Natl. Acad. Sci. U.S.A. 88:1815-1819(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The MyoD-inducible p204 protein overcomes the inhibition of myoblast differentiation by Id proteins."
Liu C.-J., Ding B., Wang H., Lengyel P.
Mol. Cell. Biol. 22:2893-2905(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH IFI204.
[3]"Ubiquitin-dependent degradation of Id1 and Id3 is mediated by the COP9 signalosome."
Berse M., Bounpheng M., Huang X., Christy B., Pollmann C., Dubiel W.
J. Mol. Biol. 343:361-370(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH COPS5 AND COPS7A.
[4]"p204 protein overcomes the inhibition of the differentiation of P19 murine embryonal carcinoma cells to beating cardiac myocytes by Id proteins."
Ding B., Liu C.-J., Huang Y., Yu J., Kong W., Lengyel P.
J. Biol. Chem. 281:14893-14906(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH GATA4 AND NKX2-5, UBIQUITINATION, SUBCELLULAR LOCATION.
[5]"Pax3/7BP is a Pax7- and Pax3-binding protein that regulates the proliferation of muscle precursor cells by an epigenetic mechanism."
Diao Y., Guo X., Li Y., Sun K., Lu L., Jiang L., Fu X., Zhu H., Sun H., Wang H., Wu Z.
Cell Stem Cell 11:231-241(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Ankyrin repeat domain protein 2 and inhibitor of DNA binding 3 cooperatively inhibit myoblast differentiation by physical interaction."
Mohamed J.S., Lopez M.A., Cox G.A., Boriek A.M.
J. Biol. Chem. 288:24560-24568(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN MYOBLAST DIFFERENTIATION, INTERACTION WITH ANKRD2, TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M60523 mRNA. Translation: AAA37818.1.
CCDSCCDS18800.1.
PIRA39114.
RefSeqNP_032347.1. NM_008321.2.
UniGeneMm.110.

3D structure databases

ProteinModelPortalP41133.
SMRP41133. Positions 32-83.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid200507. 10 interactions.
IntActP41133. 4 interactions.
STRING10090.ENSMUSP00000008016.

PTM databases

PhosphoSiteP41133.

Proteomic databases

PRIDEP41133.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000008016; ENSMUSP00000008016; ENSMUSG00000007872.
GeneID15903.
KEGGmmu:15903.
UCSCuc012dnf.1. mouse.

Organism-specific databases

CTD3399.
MGIMGI:96398. Id3.

Phylogenomic databases

eggNOGNOG83634.
HOGENOMHOG000234788.
HOVERGENHBG009009.
InParanoidP41133.
KOK17694.
OMAVISKDKR.
OrthoDBEOG7X9G8G.
PhylomeDBP41133.
TreeFamTF326217.

Gene expression databases

ArrayExpressP41133.
BgeeP41133.
CleanExMM_ID3.
GenevestigatorP41133.

Family and domain databases

Gene3D4.10.280.10. 1 hit.
InterProIPR011598. bHLH_dom.
IPR026052. DNA-bd_prot-inh.
[Graphical view]
PANTHERPTHR11723. PTHR11723. 1 hit.
PfamPF00010. HLH. 1 hit.
[Graphical view]
SMARTSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMSSF47459. SSF47459. 1 hit.
PROSITEPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio288628.
PROP41133.
SOURCESearch...

Entry information

Entry nameID3_MOUSE
AccessionPrimary (citable) accession number: P41133
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: July 9, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot