Reviewed, UniProtKB/Swiss-Prot P41131 (AMYA_AERHY)

Names and origin

Protein names

Recommended name:
    Alpha-amylase
    EC=3.2.1.1
Alternative name(s):
    1,4-alpha-D-glucan glucanohydrolase

Gene names

Name:

amyA

Organism

Aeromonas hydrophila

Taxonomic identifier

644 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Aeromonadales › Aeromonadaceae › Aeromonas

Protein attributes

Sequence length	443 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in oligosaccharides and polysaccharides.
Subcellular location	Secreted.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alpha-amylase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 443	419	Alpha-amylase		PRO_0000001327
Sites
	Active site	198	1	Nucleophile By similarity
	Active site	288	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P41131-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 8F8D60B9341A92F9	FASTA	443	48,333
	10 20 30 40 50 60 MHNTLFRTAL LAAALGSFSH TASAEGVMVH LFEWKFNDIA NECETVLGPK GFGGVQVSPP 70 80 90 100 110 120 AEHKQGSQVW WTVYQPVSYK NFNSFGGCEA ELRSMIARCN AAGVKVYADA VFNHMASGSG 130 140 150 160 170 180 TATGGGSYNS GQYQYPQFGY NDFHHSGDIT NYGDSNNVWN GALYGLPDLN TGSSYVQDQI 190 200 210 220 230 240 ATYMKTLLGW GVAGFRIDAA KHMAPADVKA ILDKAGSPRA YLEVIGAGGE SPDIQPGRYT 250 260 270 280 290 300 YIDTVTEFKY GTDLAANFNG QIKNLKTLGE SWGLLPSNKA FVFVDNHDPE RAHGGGGMLT 310 320 330 340 350 360 FMQGARYDLA NTFMLAWPYG WKQVMSAYRF ENMGTYETDK GAPGSTPCTD SQWNCEQRRP 370 380 390 400 410 420 TIMNMVLFRN RTEGQPVSNW WDNGNNQIAF SRGTRASSPS TTRAARWWPR CRRSRPASTA 430 440 TSWGAMTTAA AVMSPSTAAA RPA

References

[1]	"Cloning and nucleotide sequence of an extracellular alpha-amylase gene from Aeromonas hydrophila MCC-1." Chang M.C., Chang J.C., Chen J.P. J. Gen. Microbiol. 139:3215-3223(1993) [PubMed: 8126440] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: MCC-1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L19299 Genomic DNA. Translation: AAA21016.1.
PIR	I39538.
3D structure databases
HSSP	HSSP built from PDB template 1B0I based on UniProtKB P29957.
ModBase	Search...
Protein family/group databases
CAZy	GH13. Glycoside Hydrolase Family 13.
Enzyme and pathway databases
BRENDA	3.2.1.1. 3060.
Family and domain databases
InterPro	IPR006048. A-amylase_b_C. IPR006046. Glyco_hydro_13. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat. IPR006589. Glyco_hydro_13_sub_cat. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. [Graphical view]
PRINTS	PR00110. ALPHAAMYLASE.
SMART	SM00642. Aamy. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMYA_AERHY

Accession

Primary (citable) accession number: P41131

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	June 16, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)