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P41121

- PNP_PHOLU

UniProt

P41121 - PNP_PHOLU

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Protein

Polyribonucleotide nucleotidyltransferase

Gene
pnp, pph
Organism
Photorhabdus luminescens (Xenorhabdus luminescens)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi486 – 4861Magnesium By similarity
Metal bindingi492 – 4921Magnesium By similarity

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. response to stress Source: UniProtKB-KW
  3. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Stress response

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferase (EC:2.7.7.8)
Alternative name(s):
Polynucleotide phosphorylase
Short name:
PNPase
Gene namesi
Name:pnp
Synonyms:pph
OrganismiPhotorhabdus luminescens (Xenorhabdus luminescens)
Taxonomic identifieri29488 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaePhotorhabdus

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 709709Polyribonucleotide nucleotidyltransferaseUniRule annotationPRO_0000197915Add
BLAST

Proteomic databases

PRIDEiP41121.

Expressioni

Inductioni

In response to low temperature.UniRule annotation

Interactioni

Subunit structurei

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation By similarity.

Structurei

3D structure databases

ProteinModelPortaliP41121.
SMRiP41121. Positions 617-692.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini553 – 61260KHAdd
BLAST
Domaini622 – 69069S1 motifAdd
BLAST

Sequence similaritiesi

Contains 1 KH domain.
Contains 1 S1 motif domain.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P41121-1 [UniParc]FASTAAdd to Basket

« Hide

MLNPIVRKFQ YGQHTVTIET GMMARQATAA VMVNMDDTAV FVTVVGQKKV    50
KAGQDFFPLT VNYQERTYAA GRIPGSFFRR EGRPGEGETL VARLIDRPLR 100
PLFPEGFLNE VRIVATVVSV NPQINPDIVA MIGASAALAL SGIPFNGPIG 150
AARVGYINDQ YVLNPTSDEL KNSRLDLVVS GTAGAVLMVE SEADLLTEEQ 200
MLGAVVFGHD QQQVVIDNIN ALAAEAGKEK WDWVPEPVNQ ALHDRVAELA 250
ESRLGDAYRI TEKQERYAQV DAIKDEVTAA LLEQDETLEE AEIHEILGSL 300
EKNVVRSRVL SGEPRIDGRE KDMVRALDVR TGVLPRTHGS ALFTRGETQA 350
LVTATLGTER DAQIIDELMG ERTDRFLLHY NFPPYSVGET GMMGSPKRRE 400
IGHGRLAKRG VLAVMPKANE FPYTVRVVSE ITESNGSSSM ASVCGASLAL 450
MDAGVPIKAA VAGIAMGLVK EGDNFVVLSD ILGDEDHLGD MDFKVAGSCE 500
GISALQMDIK IEGITREIMQ VALNQAKGAR LHILSVMEQA ITTPRDDISQ 550
FAPRIHTIKI NPDKIKDVIG KGGSVIRALT EETGTTIEIE DDGTVKIAAT 600
DGEKAKHAIS RIEEITAEIE VGRIYAGKVT RIVDFGAFVA IGGGKEGLVH 650
ISQIADKRVE KVADYLQVGQ ETSVKVLEID RQGRVRLSIK EATAGTAVEE 700
APPAPQSAE 709
Length:709
Mass (Da):76,745
Last modified:February 1, 1995 - v1
Checksum:iEBFCC559FC921E6B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X76069 Genomic DNA. Translation: CAA53671.1.
PIRiS38883.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X76069 Genomic DNA. Translation: CAA53671.1 .
PIRi S38883.

3D structure databases

ProteinModelPortali P41121.
SMRi P41121. Positions 617-692.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi P41121.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The gene coding for polynucleotide phosphorylase in Photorhabdus sp. strain K122 is induced at low temperatures."
    Clarke D.J., Dowds B.C.A.
    J. Bacteriol. 176:3775-3784(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-13.
    Strain: K122.

Entry informationi

Entry nameiPNP_PHOLU
AccessioniPrimary (citable) accession number: P41121
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: April 16, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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