Reviewed, UniProtKB/Swiss-Prot P41091 (IF2G_HUMAN)

Names and origin

Protein names

Recommended name:
    Eukaryotic translation initiation factor 2 subunit 3
Alternative name(s):
    Eukaryotic translation initiation factor 2 subunit gamma
      Short name=eIF-2-gamma

Gene names

Name:	EIF2S3
Synonyms:	EIF2G

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	472 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.
Subunit structure	Heterotrimer composed of an alpha, a beta and a gamma chain.
Sequence similarities	Belongs to the GTP-binding elongation factor family. EIF2G subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Coding sequence diversity	Polymorphism
Ligand	GTP-binding Nucleotide-binding
Molecular function	Initiation factor
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	translational initiation Inferred from direct assay. Source: UniProtKB
Cellular component	cytosol Inferred from Experiment. Source: Reactome
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Ref.1 Traceable author statement. Source: ProtInc protein binding Inferred from physical interaction. Source: IntAct translation initiation factor activity Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CASP4	P49662	1	EBI-1054228,EBI-1057327
EIF1B	O60739	1	EBI-1054228,EBI-1043343
EIF2S1	P05198	1	EBI-1054228,EBI-1056162
EIF2S2	P20042	1	EBI-1054228,EBI-711977
NDRG1	Q92597	1	EBI-1054228,EBI-716486

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.3 Ref.4
	Chain	2 – 472	471	Eukaryotic translation initiation factor 2 subunit 3		PRO_0000137438
Regions
	Nucleotide binding	48 – 55	8	GTP By similarity
	Nucleotide binding	134 – 138	5	GTP By similarity
	Nucleotide binding	190 – 193	4	GTP By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine; partial Ref.4
	Modified residue	22	1	Phosphothreonine By similarity
Natural variations
	Natural variant	125	1	K → R: dbSNP rs16997659.		VAR_002352

Sequences

	Sequence		Length	Mass (Da)
	P41091-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 7A292A6AAF6DF983	FASTA	472	51,109
	10 20 30 40 50 60 MAGGEAGVTL GQPHLSRQDL TTLDVTKLTP LSHEVISRQA TINIGTIGHV AHGKSTVVKA 70 80 90 100 110 120 ISGVHTVRFK NELERNITIK LGYANAKIYK LDDPSCPRPE CYRSCGSSTP DEFPTDIPGT 130 140 150 160 170 180 KGNFKLVRHV SFVDCPGHDI LMATMLNGAA VMDAALLLIA GNESCPQPQT SEHLAAIEIM 190 200 210 220 230 240 KLKHILILQN KIDLVKESQA KEQYEQILAF VQGTVAEGAP IIPISAQLKY NIEVVCEYIV 250 260 270 280 290 300 KKIPVPPRDF TSEPRLIVIR SFDVNKPGCE VDDLKGGVAG GSILKGVLKV GQEIEVRPGI 310 320 330 340 350 360 VSKDSEGKLM CKPIFSKIVS LFAEHNDLQY AAPGGLIGVG TKIDPTLCRA DRMVGQVLGA 370 380 390 400 410 420 VGALPEIFTE LEISYFLLRR LLGVRTEGDK KAAKVQKLSK NEVLMVNIGS LSTGGRVSAV 430 440 450 460 470 KADLGKIVLT NPVCTEVGEK IALSRRVEKH WRLIGWGQIR RGVTIKPTVD DD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Translation initiation factor eIF-2. Cloning and expression of the human cDNA encoding the gamma-subunit." Gaspar N.J., Kinzy T.G., Scherer B.J., Huembelin M., Hershey J.W.B., Merrick W.C. J. Biol. Chem. 269:3415-3422(1994) [PubMed: 8106381] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Leukemia.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lymph.
[3]	"Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides." Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J. Nat. Biotechnol. 21:566-569(2003) [PubMed: 12665801] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-17. Tissue: Platelet.
[4]	Bienvenut W.V., Boldt K., von Kriegsheim A., Kolch W., Heiserich L., Gottlieb E., Vousden K.H., Lukashchuk N., Lilla S., Lempens A. Submitted (DEC-2008) to UniProtKB Cited for: PROTEIN SEQUENCE OF 2-54; 60-75; 182-191; 242-255; 276-285; 290-303; 318-342; 401-416; 453-460 AND 462-472, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, MASS SPECTROMETRY. Tissue: Colon carcinoma, Hepatoma, Lung carcinoma and Ovarian carcinoma.
[5]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L19161 mRNA. Translation: AAA19696.1. BC019906 mRNA. Translation: AAH19906.1.
IPI	IPI00297982.
PIR	A53048.
RefSeq	NP_001406.1.
UniGene	Hs.539684
3D structure databases
HSSP	HSSP built from PDB template 1KK1 based on UniProtKB Q9V1G0.
ModBase	Search...
Protein-protein interaction databases
IntAct	P41091. 11 interactions.
PTM databases
PhosphoSite	P41091.
Proteomic databases
PeptideAtlas	P41091.
PRIDE	P41091.
Genome annotation databases
Ensembl	ENSG00000130741. Homo sapiens. [Contig view]
GeneID	1968.
KEGG	hsa:1968.
NMPDR	fig|9606.3.peg.32560.
UCSC	uc004dbc.1. human.
Organism-specific databases
GeneCards	GC0XP023982.
H-InvDB	HIX0016706.
HGNC	HGNC:3267. EIF2S3.
MIM	300161. gene.
PharmGKB	PA27697.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	P41091.
HOVERGEN	P41091.
OMA	P41091. ENAPIIP.
Enzyme and pathway databases
Reactome	REACT_17015. Metabolism of proteins. REACT_1762. 3' -UTR-mediated translational regulation. REACT_71. Gene Expression.
Gene expression databases
ArrayExpress	P41091.
Bgee	P41091.
CleanEx	HS_EIF2S3.
GermOnline	ENSG00000130741. Homo sapiens.
Family and domain databases
InterPro	IPR015256. eIF2_C. IPR000795. ProtSyn_GTP_bd. IPR004161. Transl_elong_EFTu/EF1A_2. [Graphical view]
Pfam	PF09173. eIF2_C. 1 hit. PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
ProtoNet	Search...
Other Resources
NextBio	7979.
SOURCE	Search...

Entry information

Entry name

IF2G_HUMAN

Accession

Primary (citable) accession number: P41091

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	January 23, 2007
Last modified:	July 7, 2009
This is version 86 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)