Reviewed, UniProtKB/Swiss-Prot P41090 (FL3H_VITVI)

Names and origin

Protein names

Recommended name:
    Naringenin,2-oxoglutarate 3-dioxygenase
    EC=1.14.11.9
Alternative name(s):
    Flavonone-3-hydroxylase
      Short name=F3H
    FHT

Gene names

Name:

F3H

Organism

Vitis vinifera (Grape)

Taxonomic identifier

29760 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Vitales › Vitaceae › Vitis

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the 3-beta-hydroxylation of 2S-flavonones to 2R,3R-dihydroflavonols which are intermediates in the biosynthesis of flavonols, anthocyanidins, catechins and proanthocyanidins in plants.
Catalytic activity	A flavanone + 2-oxoglutarate + O(2) = a dihydroflavonol + succinate + CO(2).
Cofactor	Iron. Ascorbate.
Pathway	Secondary metabolite biosynthesis; flavonoid biosynthesis.
Sequence similarities	Belongs to the iron/ascorbate-dependent oxidoreductase family.

Ontologies

Keywords
Biological process	Flavonoid biosynthesis
Ligand	Iron Metal-binding Vitamin C
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	flavonoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-ascorbic acid binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW naringenin 3-dioxygenase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 364	364	Naringenin,2-oxoglutarate 3-dioxygenase		PRO_0000067290
Sites
	Metal binding	76	1	Iron Potential
	Metal binding	218	1	Iron Potential
	Metal binding	220	1	Iron Potential
	Metal binding	276	1	Iron Potential

Sequences

	Sequence		Length	Mass (Da)
	P41090-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 2C1FBEEF1EADD674	FASTA	364	40,875
	10 20 30 40 50 60 MAPTTLTALA GEKTLQSSFV RDEDERPKVA YNDFSNEIPV ISLTKESMKL AAVVDEICRK 70 80 90 100 110 120 IVEACEDWGI FQVVNHGVDS NLISEMTRLA REFFALPPEE NVRFDMSGGK KGGFIVSSHL 130 140 150 160 170 180 QGEAVQDWRE IVTYFSYPLR TRDYSRWPDK PEGWRSVTQE YSEKLMGLAC KLLEVLSEAM 190 200 210 220 230 240 DLDKDALTNA CVDMDQKVVV NFYPQCPQPD LTLGLKRHTD PGTITLLLQD QVGGLQATRD 250 260 270 280 290 300 GGKTWITVQP VEGAFVVNLG DHGHYLSNGR FKNADHQAVV NSNHSRLSIA TFQNPAPEAT 310 320 330 340 350 360 VYPLKIREGE KAVLEGPITF AEMYRRKMSK DLELARLKKL AKEQQLQDVE KAKLESKPID QILA

References

[1]

"Cloning and molecular analysis of structural genes involved in flavonoid and stilbene biosynthesis in grape (Vitis vinifera L.)." Sparvoli F., Martin C., Scienza A., Gavazzi G., Tonelli C. Plant Mol. Biol. 24:743-755(1994) [PubMed: 8193299] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Lambrusco Foglia Frastagliata.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X75965 mRNA. Translation: CAA53579.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR005123. 2OG-FeII_Oase. [Graphical view]
Pfam	PF03171. 2OG-FeII_Oxy. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FL3H_VITVI

Accession

Primary (citable) accession number: P41090

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 4, 2008
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)