P41080 (GYRA_RICPR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA gyrase subunit A
EC=5.99.1.3

Gene names

Name:	gyrA
Ordered Locus Names:	RP206

Organism

Rickettsia prowazekii (strain Madrid E)

Taxonomic identifier

272947 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Rickettsiaceae › Rickettsieae › Rickettsia › typhus group

Protein attributes

Sequence length	905 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings. HAMAP MF_01897
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP MF_01897
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Subcellular location	Cytoplasm Potential HAMAP MF_01897.
Sequence similarities	Belongs to the topoisomerase GyrA/ParC subunit family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 905	905	DNA gyrase subunit A HAMAP MF_01897		PRO_0000145247
Sites
	Active site	123	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P41080 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: A715C49A5A2328B6	FASTA	905	101,081
	10 20 30 40 50 60 MIDKYSSNLV PVNIEDEMKV SYLDYAMSVI VSRAIPDVRD GLKPVHRRII YSMYEAGNHA 70 80 90 100 110 120 SKPYRKSARI VGDVMGKYHP HGDSAIYDSL VRMAQDFSLR LPLVDGQGNF GSMDGDAAAA 130 140 150 160 170 180 MRYTESRMAK VAHKLVEDID KGTVSFNINY DGSEEEPSVL PAMFPNLLVN GSGGIAVGMA 190 200 210 220 230 240 TNIPPHNLGE IIDACCLYID NHDIEILDLL EVVKGPDFPT GSMILGISGI RSAYLTGRGS 250 260 270 280 290 300 IIMRGKAEIE NVGNSRQAII ITEIPYMVNK ARLVEKIAEM VKEKRIEGIS DLRDESNKNG 310 320 330 340 350 360 IRIFIELKKD VVAEVVLNQI YACTQLQTNF GVIMLALKDG LPKVMNLKEV IAAFVSFREV 370 380 390 400 410 420 VITNRTIYLL NKARDRAHIL LGLTIAISNI DEIIYIIKAS NDTNLAKQEL MARQWEVLDI 430 440 450 460 470 480 LPLIKLVDDK VILNERGTLS FTEVQAKAIL EMKLQRLTAM EKEKLEQDLK HLATDIAEYL 490 500 510 520 530 540 NILASRTRLL EILKEELIKV KEEFASPRLT SIEFGEFDQD IEDLIQREEM VVTVTLGGYI 550 560 570 580 590 600 KRVPLSSYRS QKRGGKGRSG LSMRDEDITT QVFVGSTHTP MLFFSNIGKV YSLKLYKLPL 610 620 630 640 650 660 SNPQGKGRPM VNILSLQENE HITNIMPLPE NQDEWDHLNI MFATAKGNIR RSDLLDFKKI 670 680 690 700 710 720 QSNGKIAIRL DEDDKLIDVK PCKEDEHILL ATKAGKALRF PVESLRIIKS RISDGVRGMK 730 740 750 760 770 780 LAKEDSVISM TVLKGINSTK EDRDAYLTVP WEKRLEIAKG EEFNLEELGV NLNADSILEM 790 800 810 820 830 840 ANSEEFILTV TENGFGKRSS AYGYRITDRG GSGIINMDIN DKTGLVVGVM PVKMDDELML 850 860 870 880 890 900 ITNSGKLIRC KLESVRITGR NTSGVILFKL DDDEKVVSVS LIAETSESEE ASELAEEGLE NDVKV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of the Rickettsia prowazekii gyrA gene." Wood D.O., Waite R.T. Gene 151:191-196(1994) [PubMed: 7828873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Madrid E.
[2]	"The genome sequence of Rickettsia prowazekii and the origin of mitochondria." Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T., Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H., Kurland C.G. Nature 396:133-140(1998) [PubMed: 9823893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Madrid E.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U02931 Genomic DNA. Translation: AAA68146.1. AJ235270 Genomic DNA. Translation: CAA14671.1.
PIR	H71731.
RefSeq	NP_220594.1. NC_000963.1.
3D structure databases
ProteinModelPortal	P41080.
SMR	P41080. Positions 31-512.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	883928.
GenomeReviews	Gene locus RP206 in contig AJ235269_GR.
KEGG	rpr:RP206.
PATRIC	17901170. VBIRicPro72556_0215.
Phylogenomic databases
HOGENOM	HBG635183.
OMA	TGRGRIY.
ProtClustDB	PRK05560.
Enzyme and pathway databases
BioCyc	RPRO272947:RP206-MONOMER.
Family and domain databases
HAMAP	MF_01897. GyrA. [Tree]
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. IPR013760. Topo_IIA_cen. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit. G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
KO	K02469.
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
SUPFAM	SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01063. GyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GYRA_RICPR

Accession

Primary (citable) accession number: P41080

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	January 25, 2012
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program