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Protein

Zinc finger protein on ecdysone puffs

Gene

Pep

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in the process of early and late gene activation, or possibly in RNA processing, for a defined set of developmentally regulated loci.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri216 – 24025C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri288 – 31023C2H2-type 2; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri319 – 34325C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri489 – 51325C2H2-type 4PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • DNA binding Source: FlyBase
  • metal ion binding Source: UniProtKB-KW
  • single-stranded DNA binding Source: FlyBase
  • single-stranded RNA binding Source: FlyBase

GO - Biological processi

  • mRNA splicing, via spliceosome Source: FlyBase
  • regulation of mRNA splicing, via spliceosome Source: FlyBase
  • sensory perception of pain Source: FlyBase
Complete GO annotation...

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein on ecdysone puffs
Gene namesi
Name:Pep
ORF Names:CG6143
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0004401. Pep.

Subcellular locationi

  • Nucleus 1 Publication
  • Chromosome 1 Publication

  • Note: It is associated with the active ecdysone-regulated loci on polytene chromosomes, and on some heat shock-induced puffs. Its distribution pattern follows the changes of puffing patterns in the developmental program, or following heat shock.

GO - Cellular componenti

  • catalytic step 2 spliceosome Source: FlyBase
  • chromosome Source: UniProtKB-SubCell
  • microtubule associated complex Source: FlyBase
  • nucleus Source: FlyBase
  • precatalytic spliceosome Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 716716Zinc finger protein on ecdysone puffsPRO_0000047016Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei201 – 2011Phosphoserine1 Publication
Modified residuei203 – 2031Phosphothreonine1 Publication
Modified residuei206 – 2061Phosphoserine1 Publication
Modified residuei673 – 6731Phosphoserine1 Publication
Modified residuei684 – 6841Phosphoserine1 Publication
Modified residuei686 – 6861Phosphoserine1 Publication
Modified residuei692 – 6921Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP41073.
PRIDEiP41073.

PTM databases

iPTMnetiP41073.

Expressioni

Developmental stagei

Expressed both maternally and zygotically, zygotic expression is at a low and constant level thereafter.1 Publication

Gene expression databases

BgeeiP41073.
ExpressionAtlasiP41073. differential.
GenevisibleiP41073. DM.

Interactioni

Protein-protein interaction databases

BioGridi70020. 6 interactions.
DIPiDIP-20217N.
IntActiP41073. 32 interactions.
MINTiMINT-846839.
STRINGi7227.FBpp0074963.

Structurei

3D structure databases

ProteinModelPortaliP41073.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi379 – 3835Nuclear localization signalSequence analysis
Motifi544 – 5485Nuclear localization signalSequence analysis

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi7 – 166160Asn/Gly-richAdd
BLAST
Compositional biasi111 – 1166Poly-Gly
Compositional biasi163 – 1664Poly-Gly
Compositional biasi367 – 3704Poly-Glu
Compositional biasi388 – 46679Asp/Glu-rich (acidic)Add
BLAST
Compositional biasi438 – 4414Poly-Glu
Compositional biasi535 – 5384Poly-Ala
Compositional biasi550 – 63990Asp/Glu-rich (acidic)Add
BLAST
Compositional biasi641 – 70969Ala-richAdd
BLAST
Compositional biasi675 – 6784Poly-Ala

Sequence similaritiesi

Contains 4 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri216 – 24025C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri288 – 31023C2H2-type 2; atypicalPROSITE-ProRule annotationAdd
BLAST
Zinc fingeri319 – 34325C2H2-type 3PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri489 – 51325C2H2-type 4PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IZWE. Eukaryota.
ENOG4111SKG. LUCA.
InParanoidiP41073.
OMAiISKLECY.
OrthoDBiEOG7FV3PW.
PhylomeDBiP41073.

Family and domain databases

InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 3 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform B (identifier: P41073-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVSVKVNGNP QNRLVNNAKV NGNMAFRGNQ NRNRNFGGGN NNYGGPMGAN
60 70 80 90 100
RMGGMNMSPW ESQNPGGGQF GNNMRQGGGQ MNAQAINLAN NLLNNLFRNQ
110 120 130 140 150
NPPSLLDLPR GGGGMGNRNQ RGGPMVSRGG GAGNRLNNRR GQGGGFQNRG
160 170 180 190 200
ATGSGPKPPP KQGGGGIRKQ NAFDRAKKLL AKNANQNKKK EPTPGEKKIE
210 220 230 240 250
SPTKESPYAS VPNDMFYCHL CKKHMWDANS FENHIKGRTH LMMREGIEES
260 270 280 290 300
YRLKANMIRQ EAKIAEQLKS IEFDRLKRMG KSKQRQLDYC TMCDLNFHGH
310 320 330 340 350
ISTHRKSEGH LQLKKFLHPK CIECNKEFAT RIDYDTHLLS AEHLKKAAEN
360 370 380 390 400
NTKVGERKRQ TLPISTEEEE TRDLRLPQKR KKKPVKKEGE AADGEAKKEG
410 420 430 440 450
AGDGEGAEGD EAEGEEAKEG EEAADETKEG DELNESQEEE EVALPVDPED
460 470 480 490 500
CILDFNDGDE IPSEVDTRLP KYNWQRAVGP GLISKLECYE CSVCSKFFDT
510 520 530 540 550
EVTAEIHSRT ATHHRNFLKF INEKSSDTKI AQKRAAAALE ENERKKRKVE
560 570 580 590 600
EAEAPAAEGA AEETTEGAEG ELYDPSEATG DDEDVEMVDD NAEGEGEGEG
610 620 630 640 650
DEEAEAEVEE DGAGQDNGEE EMEAQEEEGQ EGEQEPEPEP APVQTPAPAE
660 670 680 690 700
PAPPAKTPAK TPTKAAAPAA VASPAAAATS ADASPSPAKK ATPARAAAGA
710
KATPQRQRAR GRYNRY
Length:716
Mass (Da):78,048
Last modified:February 1, 1995 - v1
Checksum:i256D7D765C5F3050
GO
Isoform A (identifier: P41073-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-23: Missing.
     125-132: MVSRGGGA → PYQGVSIR
     133-716: Missing.

Note: No experimental confirmation available.
Show »
Length:109
Mass (Da):11,606
Checksum:i9D8A14944E7032B9
GO
Isoform C (identifier: P41073-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-23: Missing.

Note: No experimental confirmation available.
Show »
Length:693
Mass (Da):75,599
Checksum:iCFC531F249069DDD
GO

Sequence cautioni

The sequence AAN71637.1 differs from that shown.Intron retention.Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2323Missing in isoform A and isoform C. 1 PublicationVSP_009605Add
BLAST
Alternative sequencei125 – 1328MVSRGGGA → PYQGVSIR in isoform A. CuratedVSP_037472
Alternative sequencei133 – 716584Missing in isoform A. CuratedVSP_037473Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56689 mRNA. Translation: CAA40017.1.
AE014296 Genomic DNA. Translation: AAF49317.4.
AE014296 Genomic DNA. Translation: AAF49319.3.
AE014296 Genomic DNA. Translation: AAS64975.1.
AY118552 mRNA. Translation: AAM49921.1.
BT001867 mRNA. Translation: AAN71637.1. Sequence problems.
PIRiS26759.
RefSeqiNP_001246817.1. NM_001259888.3. [P41073-3]
NP_524858.1. NM_080119.6. [P41073-1]
NP_730290.3. NM_168742.4. [P41073-2]
NP_996118.1. NM_206396.4. [P41073-3]
UniGeneiDm.6853.

Genome annotation databases

EnsemblMetazoaiFBtr0075199; FBpp0074963; FBgn0004401. [P41073-1]
GeneIDi45961.
KEGGidme:Dmel_CG6143.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56689 mRNA. Translation: CAA40017.1.
AE014296 Genomic DNA. Translation: AAF49317.4.
AE014296 Genomic DNA. Translation: AAF49319.3.
AE014296 Genomic DNA. Translation: AAS64975.1.
AY118552 mRNA. Translation: AAM49921.1.
BT001867 mRNA. Translation: AAN71637.1. Sequence problems.
PIRiS26759.
RefSeqiNP_001246817.1. NM_001259888.3. [P41073-3]
NP_524858.1. NM_080119.6. [P41073-1]
NP_730290.3. NM_168742.4. [P41073-2]
NP_996118.1. NM_206396.4. [P41073-3]
UniGeneiDm.6853.

3D structure databases

ProteinModelPortaliP41073.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi70020. 6 interactions.
DIPiDIP-20217N.
IntActiP41073. 32 interactions.
MINTiMINT-846839.
STRINGi7227.FBpp0074963.

PTM databases

iPTMnetiP41073.

Proteomic databases

PaxDbiP41073.
PRIDEiP41073.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0075199; FBpp0074963; FBgn0004401. [P41073-1]
GeneIDi45961.
KEGGidme:Dmel_CG6143.

Organism-specific databases

CTDi45961.
FlyBaseiFBgn0004401. Pep.

Phylogenomic databases

eggNOGiENOG410IZWE. Eukaryota.
ENOG4111SKG. LUCA.
InParanoidiP41073.
OMAiISKLECY.
OrthoDBiEOG7FV3PW.
PhylomeDBiP41073.

Miscellaneous databases

GenomeRNAii45961.
PROiP41073.

Gene expression databases

BgeeiP41073.
ExpressionAtlasiP41073. differential.
GenevisibleiP41073. DM.

Family and domain databases

InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 3 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A unique zinc finger protein is associated preferentially with active ecdysone-responsive loci in Drosophila."
    Amero S.A., Elgin S.C.R., Beyer A.L.
    Genes Dev. 5:188-200(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B), FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
    Strain: Oregon-R.
    Tissue: Embryo.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM C), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 202-716 (ISOFORMS B/C).
    Strain: Berkeley.
    Tissue: Embryo.
  5. "Phosphoproteome analysis of Drosophila melanogaster embryos."
    Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
    J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-201; THR-203; SER-206; SER-673; SER-684; SER-686 AND THR-692, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Embryo.

Entry informationi

Entry nameiPEP_DROME
AccessioniPrimary (citable) accession number: P41073
Secondary accession number(s): Q8IGB1
, Q8MSV5, Q9VVJ2, Q9VVJ3, Q9VVJ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: July 6, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.