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Protein

Zinc finger protein on ecdysone puffs

Gene

Pep

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in the process of early and late gene activation, or possibly in RNA processing, for a defined set of developmentally regulated loci.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri216 – 240C2H2-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri288 – 310C2H2-type 2; atypicalPROSITE-ProRule annotationAdd BLAST23
Zinc fingeri319 – 343C2H2-type 3PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri489 – 513C2H2-type 4PROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

  • DNA binding Source: FlyBase
  • metal ion binding Source: UniProtKB-KW
  • single-stranded DNA binding Source: FlyBase
  • single-stranded RNA binding Source: FlyBase

GO - Biological processi

  • mRNA splicing, via spliceosome Source: FlyBase
  • regulation of mRNA splicing, via spliceosome Source: FlyBase
  • sensory perception of pain Source: FlyBase
Complete GO annotation...

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein on ecdysone puffs
Gene namesi
Name:Pep
ORF Names:CG6143
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0004401. Pep.

Subcellular locationi

  • Nucleus 1 Publication
  • Chromosome 1 Publication

  • Note: It is associated with the active ecdysone-regulated loci on polytene chromosomes, and on some heat shock-induced puffs. Its distribution pattern follows the changes of puffing patterns in the developmental program, or following heat shock.

GO - Cellular componenti

  • catalytic step 2 spliceosome Source: FlyBase
  • chromosome Source: UniProtKB-SubCell
  • microtubule associated complex Source: FlyBase
  • precatalytic spliceosome Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000470161 – 716Zinc finger protein on ecdysone puffsAdd BLAST716

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei201Phosphoserine1 Publication1
Modified residuei203Phosphothreonine1 Publication1
Modified residuei206Phosphoserine1 Publication1
Modified residuei673Phosphoserine1 Publication1
Modified residuei684Phosphoserine1 Publication1
Modified residuei686Phosphoserine1 Publication1
Modified residuei692Phosphothreonine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP41073.
PRIDEiP41073.

PTM databases

iPTMnetiP41073.

Expressioni

Developmental stagei

Expressed both maternally and zygotically, zygotic expression is at a low and constant level thereafter.1 Publication

Gene expression databases

BgeeiFBgn0004401.
ExpressionAtlasiP41073. baseline.
GenevisibleiP41073. DM.

Interactioni

Protein-protein interaction databases

BioGridi70020. 6 interactors.
DIPiDIP-20217N.
IntActiP41073. 32 interactors.
MINTiMINT-846839.
STRINGi7227.FBpp0074963.

Structurei

3D structure databases

ProteinModelPortaliP41073.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi379 – 383Nuclear localization signalSequence analysis5
Motifi544 – 548Nuclear localization signalSequence analysis5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi7 – 166Asn/Gly-richAdd BLAST160
Compositional biasi111 – 116Poly-Gly6
Compositional biasi163 – 166Poly-Gly4
Compositional biasi367 – 370Poly-Glu4
Compositional biasi388 – 466Asp/Glu-rich (acidic)Add BLAST79
Compositional biasi438 – 441Poly-Glu4
Compositional biasi535 – 538Poly-Ala4
Compositional biasi550 – 639Asp/Glu-rich (acidic)Add BLAST90
Compositional biasi641 – 709Ala-richAdd BLAST69
Compositional biasi675 – 678Poly-Ala4

Sequence similaritiesi

Contains 4 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri216 – 240C2H2-type 1PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri288 – 310C2H2-type 2; atypicalPROSITE-ProRule annotationAdd BLAST23
Zinc fingeri319 – 343C2H2-type 3PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri489 – 513C2H2-type 4PROSITE-ProRule annotationAdd BLAST25

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IZWE. Eukaryota.
ENOG4111SKG. LUCA.
InParanoidiP41073.
OMAiISKLECY.
OrthoDBiEOG091G0A8O.
PhylomeDBiP41073.

Family and domain databases

InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 3 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform B (identifier: P41073-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVSVKVNGNP QNRLVNNAKV NGNMAFRGNQ NRNRNFGGGN NNYGGPMGAN
60 70 80 90 100
RMGGMNMSPW ESQNPGGGQF GNNMRQGGGQ MNAQAINLAN NLLNNLFRNQ
110 120 130 140 150
NPPSLLDLPR GGGGMGNRNQ RGGPMVSRGG GAGNRLNNRR GQGGGFQNRG
160 170 180 190 200
ATGSGPKPPP KQGGGGIRKQ NAFDRAKKLL AKNANQNKKK EPTPGEKKIE
210 220 230 240 250
SPTKESPYAS VPNDMFYCHL CKKHMWDANS FENHIKGRTH LMMREGIEES
260 270 280 290 300
YRLKANMIRQ EAKIAEQLKS IEFDRLKRMG KSKQRQLDYC TMCDLNFHGH
310 320 330 340 350
ISTHRKSEGH LQLKKFLHPK CIECNKEFAT RIDYDTHLLS AEHLKKAAEN
360 370 380 390 400
NTKVGERKRQ TLPISTEEEE TRDLRLPQKR KKKPVKKEGE AADGEAKKEG
410 420 430 440 450
AGDGEGAEGD EAEGEEAKEG EEAADETKEG DELNESQEEE EVALPVDPED
460 470 480 490 500
CILDFNDGDE IPSEVDTRLP KYNWQRAVGP GLISKLECYE CSVCSKFFDT
510 520 530 540 550
EVTAEIHSRT ATHHRNFLKF INEKSSDTKI AQKRAAAALE ENERKKRKVE
560 570 580 590 600
EAEAPAAEGA AEETTEGAEG ELYDPSEATG DDEDVEMVDD NAEGEGEGEG
610 620 630 640 650
DEEAEAEVEE DGAGQDNGEE EMEAQEEEGQ EGEQEPEPEP APVQTPAPAE
660 670 680 690 700
PAPPAKTPAK TPTKAAAPAA VASPAAAATS ADASPSPAKK ATPARAAAGA
710
KATPQRQRAR GRYNRY
Length:716
Mass (Da):78,048
Last modified:February 1, 1995 - v1
Checksum:i256D7D765C5F3050
GO
Isoform A (identifier: P41073-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-23: Missing.
     125-132: MVSRGGGA → PYQGVSIR
     133-716: Missing.

Note: No experimental confirmation available.
Show »
Length:109
Mass (Da):11,606
Checksum:i9D8A14944E7032B9
GO
Isoform C (identifier: P41073-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-23: Missing.

Note: No experimental confirmation available.
Show »
Length:693
Mass (Da):75,599
Checksum:iCFC531F249069DDD
GO

Sequence cautioni

The sequence AAN71637 differs from that shown. Intron retention.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0096051 – 23Missing in isoform A and isoform C. 1 PublicationAdd BLAST23
Alternative sequenceiVSP_037472125 – 132MVSRGGGA → PYQGVSIR in isoform A. Curated8
Alternative sequenceiVSP_037473133 – 716Missing in isoform A. CuratedAdd BLAST584

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56689 mRNA. Translation: CAA40017.1.
AE014296 Genomic DNA. Translation: AAF49317.4.
AE014296 Genomic DNA. Translation: AAF49319.3.
AE014296 Genomic DNA. Translation: AAS64975.1.
AY118552 mRNA. Translation: AAM49921.1.
BT001867 mRNA. Translation: AAN71637.1. Sequence problems.
PIRiS26759.
RefSeqiNP_001246817.1. NM_001259888.3. [P41073-3]
NP_524858.1. NM_080119.6. [P41073-1]
NP_730290.3. NM_168742.4. [P41073-2]
NP_996118.1. NM_206396.4. [P41073-3]
UniGeneiDm.6853.

Genome annotation databases

EnsemblMetazoaiFBtr0075199; FBpp0074963; FBgn0004401. [P41073-1]
GeneIDi45961.
KEGGidme:Dmel_CG6143.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56689 mRNA. Translation: CAA40017.1.
AE014296 Genomic DNA. Translation: AAF49317.4.
AE014296 Genomic DNA. Translation: AAF49319.3.
AE014296 Genomic DNA. Translation: AAS64975.1.
AY118552 mRNA. Translation: AAM49921.1.
BT001867 mRNA. Translation: AAN71637.1. Sequence problems.
PIRiS26759.
RefSeqiNP_001246817.1. NM_001259888.3. [P41073-3]
NP_524858.1. NM_080119.6. [P41073-1]
NP_730290.3. NM_168742.4. [P41073-2]
NP_996118.1. NM_206396.4. [P41073-3]
UniGeneiDm.6853.

3D structure databases

ProteinModelPortaliP41073.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi70020. 6 interactors.
DIPiDIP-20217N.
IntActiP41073. 32 interactors.
MINTiMINT-846839.
STRINGi7227.FBpp0074963.

PTM databases

iPTMnetiP41073.

Proteomic databases

PaxDbiP41073.
PRIDEiP41073.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0075199; FBpp0074963; FBgn0004401. [P41073-1]
GeneIDi45961.
KEGGidme:Dmel_CG6143.

Organism-specific databases

CTDi45961.
FlyBaseiFBgn0004401. Pep.

Phylogenomic databases

eggNOGiENOG410IZWE. Eukaryota.
ENOG4111SKG. LUCA.
InParanoidiP41073.
OMAiISKLECY.
OrthoDBiEOG091G0A8O.
PhylomeDBiP41073.

Miscellaneous databases

GenomeRNAii45961.
PROiP41073.

Gene expression databases

BgeeiFBgn0004401.
ExpressionAtlasiP41073. baseline.
GenevisibleiP41073. DM.

Family and domain databases

InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 3 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 3 hits.
PS50157. ZINC_FINGER_C2H2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPEP_DROME
AccessioniPrimary (citable) accession number: P41073
Secondary accession number(s): Q8IGB1
, Q8MSV5, Q9VVJ2, Q9VVJ3, Q9VVJ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 30, 2016
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.