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Protein

40S ribosomal protein S29-A

Gene

RPS29A

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel.1 Publication

Miscellaneous

Present with 6490 molecules/cell in log phase SD medium.1 Publication
There are 2 genes for uS14 in yeast.Curated

Cofactori

Zn2+CuratedNote: Binds 1 zinc ion per subunit.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi21ZincSequence analysis1
Metal bindingi24ZincSequence analysis1
Metal bindingi39ZincSequence analysis1
Metal bindingi42ZincSequence analysis1

GO - Molecular functioni

  • structural constituent of ribosome Source: SGD
  • zinc ion binding Source: GO_Central

GO - Biological processi

  • cytoplasmic translation Source: SGD

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein
LigandMetal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-32454-MONOMER
ReactomeiR-SCE-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-SCE-1799339 SRP-dependent cotranslational protein targeting to membrane
R-SCE-72689 Formation of a pool of free 40S subunits
R-SCE-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-SCE-72702 Ribosomal scanning and start codon recognition
R-SCE-72706 GTP hydrolysis and joining of the 60S ribosomal subunit
R-SCE-975956 Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)
R-SCE-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Names & Taxonomyi

Protein namesi
Recommended name:
40S ribosomal protein S29-A1 Publication
Alternative name(s):
S36
Small ribosomal subunit protein uS14-A1 Publication
YS29
Gene namesi
Name:RPS29A1 Publication
Synonyms:RPS36A, YS29A
Ordered Locus Names:YLR388W
ORF Names:L8084.11
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR388W
SGDiS000004380 RPS29A

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00001310322 – 5640S ribosomal protein S29-AAdd BLAST55

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei25PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP41057
PaxDbiP41057
PRIDEiP41057

PTM databases

iPTMnetiP41057

Interactioni

Subunit structurei

Component of the small ribosomal subunit (SSU). Mature yeast ribosomes consist of a small (40S) and a large (60S) subunit. The 40S small subunit contains 1 molecule of ribosomal RNA (18S rRNA) and 33 different proteins (encoded by 57 genes). The large 60S subunit contains 3 rRNA molecules (25S, 5.8S and 5S rRNA) and 46 different proteins (encoded by 81 genes) (PubMed:9559554, PubMed:22096102).1 Publication1 Publication

Protein-protein interaction databases

BioGridi31647, 197 interactors
DIPiDIP-4643N
IntActiP41057, 5 interactors
STRINGi4932.YLR388W

Structurei

Secondary structure

156
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 8Combined sources3
Beta strandi13 – 15Combined sources3
Helixi16 – 18Combined sources3
Turni22 – 24Combined sources3
Beta strandi28 – 31Combined sources4
Helixi33 – 35Combined sources3
Helixi40 – 50Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3J6Xelectron microscopy6.10291-56[»]
3J6Yelectron microscopy6.10291-56[»]
3J77electron microscopy6.20291-56[»]
3J78electron microscopy6.30291-56[»]
4U3MX-ray3.00D9/d92-56[»]
4U3NX-ray3.20D9/d92-56[»]
4U3UX-ray2.90D9/d92-56[»]
4U4NX-ray3.10D9/d92-56[»]
4U4OX-ray3.60D9/d92-56[»]
4U4QX-ray3.00D9/d92-56[»]
4U4RX-ray2.80D9/d92-56[»]
4U4UX-ray3.00D9/d92-56[»]
4U4YX-ray3.20D9/d92-56[»]
4U4ZX-ray3.10D9/d92-56[»]
4U50X-ray3.20D9/d92-56[»]
4U51X-ray3.20D9/d92-56[»]
4U52X-ray3.00D9/d92-56[»]
4U53X-ray3.30D9/d92-56[»]
4U55X-ray3.20D9/d92-56[»]
4U56X-ray3.45D9/d92-56[»]
4U6FX-ray3.10D9/d92-56[»]
4V6Ielectron microscopy8.80AN1-56[»]
4V7RX-ray4.00AS/CS1-56[»]
4V88X-ray3.00Ad/Cd1-56[»]
4V8Yelectron microscopy4.30A31-56[»]
4V8Zelectron microscopy6.60A31-56[»]
5DATX-ray3.15D9/d92-56[»]
5DC3X-ray3.25D9/d92-56[»]
5DGEX-ray3.45D9/d92-56[»]
5DGFX-ray3.30D9/d92-56[»]
5DGVX-ray3.10D9/d92-56[»]
5FCIX-ray3.40D9/d92-56[»]
5FCJX-ray3.10D9/d92-56[»]
5I4LX-ray3.10D9/d94-56[»]
5JUOelectron microscopy4.00AC1-56[»]
5JUPelectron microscopy3.50AC1-56[»]
5JUSelectron microscopy4.20AC1-56[»]
5JUTelectron microscopy4.00AC1-56[»]
5JUUelectron microscopy4.00AC1-56[»]
5LYBX-ray3.25D9/d94-56[»]
5M1Jelectron microscopy3.30d24-56[»]
5MC6electron microscopy3.80M1-56[»]
5MEIX-ray3.50d9/e4-56[»]
5NDGX-ray3.70D9/d94-56[»]
5NDVX-ray3.30D9/d94-56[»]
5NDWX-ray3.70D9/d94-56[»]
5OBMX-ray3.40D9/d94-56[»]
5ON6X-ray3.10d9/e4-56[»]
5TBWX-ray3.00d9/e4-56[»]
5TGAX-ray3.30D9/d94-56[»]
5TGMX-ray3.50D9/d94-56[»]
6FAIelectron microscopy3.40d1-56[»]
ProteinModelPortaliP41057
SMRiP41057
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000005814
HOGENOMiHOG000231300
InParanoidiP41057
KOiK02980
OMAiHCFREIA
OrthoDBiEOG092C5WBL

Family and domain databases

InterProiView protein in InterPro
IPR001209 Ribosomal_S14
IPR018271 Ribosomal_S14_CS
PfamiView protein in Pfam
PF00253 Ribosomal_S14, 1 hit
PROSITEiView protein in PROSITE
PS00527 RIBOSOMAL_S14, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P41057-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAHENVWFSH PRRYGKGSRQ CRVCSSHTGL IRKYGLNICR QCFREKANDI

GFNKFR
Length:56
Mass (Da):6,661
Last modified:January 23, 2007 - v3
Checksum:iF1A6900EE27748C2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti7W → G AA sequence (PubMed:18782943).Curated1
Sequence conflicti14Y → F in ABM97475 (PubMed:17244705).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D14676 Genomic DNA Translation: BAA03507.1
U19729 Genomic DNA Translation: AAB82350.1
EF123131 mRNA Translation: ABM97475.1
BK006945 Genomic DNA Translation: DAA09689.1
PIRiS48503
RefSeqiNP_013492.3, NM_001182277.3

Genome annotation databases

EnsemblFungiiYLR388W; YLR388W; YLR388W
GeneIDi851104
KEGGisce:YLR388W

Similar proteinsi

Entry informationi

Entry nameiRS29A_YEAST
AccessioniPrimary (citable) accession number: P41057
Secondary accession number(s): A2TBM8, D6VZ23, P05761
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 144 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health