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Protein

Membrane-bound lytic murein transglycosylase B

Gene

mltB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N-acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division.

Catalytic activityi

Exolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan, from either the reducing or the non-reducing ends of the peptidoglycan chains, with concomitant formation of a 1,6-anhydrobond in the MurNAc residue.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1621

GO - Molecular functioni

  • exo-oligoalginate lyase activity Source: UniProtKB-EC
  • gellan lyase activity Source: UniProtKB-EC
  • lytic transglycosylase activity Source: EcoCyc

GO - Biological processi

  • cell wall organization Source: UniProtKB-KW
  • peptidoglycan biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciEcoCyc:G7410-MONOMER.
ECOL316407:JW2671-MONOMER.
MetaCyc:G7410-MONOMER.

Protein family/group databases

CAZyiGH103. Glycoside Hydrolase Family 103.

Names & Taxonomyi

Protein namesi
Recommended name:
Membrane-bound lytic murein transglycosylase B (EC:4.2.2.n1)
Alternative name(s):
35 kDa soluble lytic transglycosylase
Murein hydrolase B
Slt35
Gene namesi
Name:mltB
Ordered Locus Names:b2701, JW2671
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12699. mltB.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2046262.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18CuratedAdd BLAST18
ChainiPRO_000003278419 – 361Membrane-bound lytic murein transglycosylase BAdd BLAST343

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi19N-palmitoyl cysteineCurated1
Lipidationi19S-diacylglycerol cysteineCurated1

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

PaxDbiP41052.
PRIDEiP41052.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi4262079. 357 interactors.
DIPiDIP-10221N.
IntActiP41052. 10 interactors.
MINTiMINT-1257117.
STRINGi511145.b2701.

Chemistry databases

BindingDBiP41052.

Structurei

Secondary structure

1361
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni53 – 56Combined sources4
Helixi58 – 71Combined sources14
Helixi75 – 82Combined sources8
Helixi89 – 95Combined sources7
Helixi113 – 118Combined sources6
Turni119 – 121Combined sources3
Helixi124 – 136Combined sources13
Helixi138 – 148Combined sources11
Helixi152 – 163Combined sources12
Turni164 – 167Combined sources4
Beta strandi171 – 173Combined sources3
Helixi174 – 183Combined sources10
Helixi186 – 188Combined sources3
Helixi189 – 205Combined sources17
Turni210 – 212Combined sources3
Turni222 – 225Combined sources4
Helixi228 – 234Combined sources7
Beta strandi239 – 242Combined sources4
Helixi249 – 262Combined sources14
Beta strandi271 – 277Combined sources7
Beta strandi280 – 282Combined sources3
Beta strandi285 – 291Combined sources7
Helixi292 – 297Combined sources6
Beta strandi301 – 304Combined sources4
Beta strandi311 – 318Combined sources8
Beta strandi320 – 328Combined sources9
Helixi330 – 336Combined sources7
Helixi342 – 360Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1D0KX-ray2.02A40-361[»]
1D0LX-ray1.97A40-361[»]
1D0MX-ray2.47A40-361[»]
1LTMX-ray1.70A42-361[»]
1QDRX-ray2.10A40-361[»]
1QDTX-ray2.10A40-361[»]
1QUSX-ray1.70A40-361[»]
1QUTX-ray2.44A40-361[»]
ProteinModelPortaliP41052.
SMRiP41052.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP41052.

Family & Domainsi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105CVE. Bacteria.
COG2951. LUCA.
HOGENOMiHOG000257828.
InParanoidiP41052.
KOiK08305.
OMAiSSIRDYA.
PhylomeDBiP41052.

Family and domain databases

InterProiIPR023346. Lysozyme-like_dom.
IPR011757. Lytic_transglycosylase_MltB.
IPR031304. SLT_2.
[Graphical view]
PANTHERiPTHR30163. PTHR30163. 1 hit.
PfamiPF13406. SLT_2. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
TIGRFAMsiTIGR02282. MltB. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P41052-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKRRYVTLL PLFVLLAACS SKPKPTETDT TTGTPSGGFL LEPQHNVMQM
60 70 80 90 100
GGDFANNPNA QQFIDKMVNK HGFDRQQLQE ILSQAKRLDS VLRLMDNQAP
110 120 130 140 150
TTSVKPPSGP NGAWLRYRKK FITPDNVQNG VVFWNQYEDA LNRAWQVYGV
160 170 180 190 200
PPEIIVGIIG VETRWGRVMG KTRILDALAT LSFNYPRRAE YFSGELETFL
210 220 230 240 250
LMARDEQDDP LNLKGSFAGA MGYGQFMPSS YKQYAVDFSG DGHINLWDPV
260 270 280 290 300
DAIGSVANYF KAHGWVKGDQ VAVMANGQAP GLPNGFKTKY SISQLAAAGL
310 320 330 340 350
TPQQPLGNHQ QASLLRLDVG TGYQYWYGLP NFYTITRYNH STHYAMAVWQ
360
LGQAVALARV Q
Length:361
Mass (Da):40,256
Last modified:February 1, 1995 - v1
Checksum:i7E98F040504F75F9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18785 Genomic DNA. Translation: AAB60060.1.
U00096 Genomic DNA. Translation: AAC75743.1.
AP009048 Genomic DNA. Translation: BAA16563.1.
J02708 Genomic DNA. No translation available.
PIRiA65050.
RefSeqiNP_417181.1. NC_000913.3.
WP_001295177.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75743; AAC75743; b2701.
BAA16563; BAA16563; BAA16563.
GeneIDi947184.
KEGGiecj:JW2671.
eco:b2701.
PATRICi32120800. VBIEscCol129921_2792.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18785 Genomic DNA. Translation: AAB60060.1.
U00096 Genomic DNA. Translation: AAC75743.1.
AP009048 Genomic DNA. Translation: BAA16563.1.
J02708 Genomic DNA. No translation available.
PIRiA65050.
RefSeqiNP_417181.1. NC_000913.3.
WP_001295177.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1D0KX-ray2.02A40-361[»]
1D0LX-ray1.97A40-361[»]
1D0MX-ray2.47A40-361[»]
1LTMX-ray1.70A42-361[»]
1QDRX-ray2.10A40-361[»]
1QDTX-ray2.10A40-361[»]
1QUSX-ray1.70A40-361[»]
1QUTX-ray2.44A40-361[»]
ProteinModelPortaliP41052.
SMRiP41052.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262079. 357 interactors.
DIPiDIP-10221N.
IntActiP41052. 10 interactors.
MINTiMINT-1257117.
STRINGi511145.b2701.

Chemistry databases

BindingDBiP41052.
ChEMBLiCHEMBL2046262.

Protein family/group databases

CAZyiGH103. Glycoside Hydrolase Family 103.

Proteomic databases

PaxDbiP41052.
PRIDEiP41052.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75743; AAC75743; b2701.
BAA16563; BAA16563; BAA16563.
GeneIDi947184.
KEGGiecj:JW2671.
eco:b2701.
PATRICi32120800. VBIEscCol129921_2792.

Organism-specific databases

EchoBASEiEB2561.
EcoGeneiEG12699. mltB.

Phylogenomic databases

eggNOGiENOG4105CVE. Bacteria.
COG2951. LUCA.
HOGENOMiHOG000257828.
InParanoidiP41052.
KOiK08305.
OMAiSSIRDYA.
PhylomeDBiP41052.

Enzyme and pathway databases

BioCyciEcoCyc:G7410-MONOMER.
ECOL316407:JW2671-MONOMER.
MetaCyc:G7410-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP41052.
PROiP41052.

Family and domain databases

InterProiIPR023346. Lysozyme-like_dom.
IPR011757. Lytic_transglycosylase_MltB.
IPR031304. SLT_2.
[Graphical view]
PANTHERiPTHR30163. PTHR30163. 1 hit.
PfamiPF13406. SLT_2. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
TIGRFAMsiTIGR02282. MltB. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMLTB_ECOLI
AccessioniPrimary (citable) accession number: P41052
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: November 30, 2016
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.