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Protein

Membrane-bound lytic murein transglycosylase B

Gene

mltB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N-acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division.

Catalytic activityi

Exolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan, from either the reducing or the non-reducing ends of the peptidoglycan chains, with concomitant formation of a 1,6-anhydrobond in the MurNAc residue.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei162 – 1621

GO - Molecular functioni

  • carbon-oxygen lyase activity, acting on polysaccharides Source: UniProtKB-EC
  • lytic transglycosylase activity Source: EcoCyc

GO - Biological processi

  • cell wall organization Source: UniProtKB-KW
  • peptidoglycan biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciEcoCyc:G7410-MONOMER.
ECOL316407:JW2671-MONOMER.
MetaCyc:G7410-MONOMER.

Protein family/group databases

CAZyiGH103. Glycoside Hydrolase Family 103.

Names & Taxonomyi

Protein namesi
Recommended name:
Membrane-bound lytic murein transglycosylase B (EC:4.2.2.n1)
Alternative name(s):
35 kDa soluble lytic transglycosylase
Murein hydrolase B
Slt35
Gene namesi
Name:mltB
Ordered Locus Names:b2701, JW2671
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12699. mltB.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2046262.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818CuratedAdd
BLAST
Chaini19 – 361343Membrane-bound lytic murein transglycosylase BPRO_0000032784Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi19 – 191N-palmitoyl cysteineCurated
Lipidationi19 – 191S-diacylglycerol cysteineCurated

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

PaxDbiP41052.
PRIDEiP41052.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

BioGridi4262079. 357 interactions.
DIPiDIP-10221N.
IntActiP41052. 10 interactions.
MINTiMINT-1257117.
STRINGi511145.b2701.

Chemistry

BindingDBiP41052.

Structurei

Secondary structure

1
361
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni53 – 564Combined sources
Helixi58 – 7114Combined sources
Helixi75 – 828Combined sources
Helixi89 – 957Combined sources
Helixi113 – 1186Combined sources
Turni119 – 1213Combined sources
Helixi124 – 13613Combined sources
Helixi138 – 14811Combined sources
Helixi152 – 16312Combined sources
Turni164 – 1674Combined sources
Beta strandi171 – 1733Combined sources
Helixi174 – 18310Combined sources
Helixi186 – 1883Combined sources
Helixi189 – 20517Combined sources
Turni210 – 2123Combined sources
Turni222 – 2254Combined sources
Helixi228 – 2347Combined sources
Beta strandi239 – 2424Combined sources
Helixi249 – 26214Combined sources
Beta strandi271 – 2777Combined sources
Beta strandi280 – 2823Combined sources
Beta strandi285 – 2917Combined sources
Helixi292 – 2976Combined sources
Beta strandi301 – 3044Combined sources
Beta strandi311 – 3188Combined sources
Beta strandi320 – 3289Combined sources
Helixi330 – 3367Combined sources
Helixi342 – 36019Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1D0KX-ray2.02A40-361[»]
1D0LX-ray1.97A40-361[»]
1D0MX-ray2.47A40-361[»]
1LTMX-ray1.70A42-361[»]
1QDRX-ray2.10A40-361[»]
1QDTX-ray2.10A40-361[»]
1QUSX-ray1.70A40-361[»]
1QUTX-ray2.44A40-361[»]
ProteinModelPortaliP41052.
SMRiP41052. Positions 40-361.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP41052.

Family & Domainsi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105CVE. Bacteria.
COG2951. LUCA.
HOGENOMiHOG000257828.
InParanoidiP41052.
KOiK08305.
OMAiSSIRDYA.
PhylomeDBiP41052.

Family and domain databases

InterProiIPR023346. Lysozyme-like_dom.
IPR011757. Lytic_transglycosylase_MltB.
IPR031304. SLT_2.
[Graphical view]
PANTHERiPTHR30163. PTHR30163. 1 hit.
PfamiPF13406. SLT_2. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
TIGRFAMsiTIGR02282. MltB. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P41052-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKRRYVTLL PLFVLLAACS SKPKPTETDT TTGTPSGGFL LEPQHNVMQM
60 70 80 90 100
GGDFANNPNA QQFIDKMVNK HGFDRQQLQE ILSQAKRLDS VLRLMDNQAP
110 120 130 140 150
TTSVKPPSGP NGAWLRYRKK FITPDNVQNG VVFWNQYEDA LNRAWQVYGV
160 170 180 190 200
PPEIIVGIIG VETRWGRVMG KTRILDALAT LSFNYPRRAE YFSGELETFL
210 220 230 240 250
LMARDEQDDP LNLKGSFAGA MGYGQFMPSS YKQYAVDFSG DGHINLWDPV
260 270 280 290 300
DAIGSVANYF KAHGWVKGDQ VAVMANGQAP GLPNGFKTKY SISQLAAAGL
310 320 330 340 350
TPQQPLGNHQ QASLLRLDVG TGYQYWYGLP NFYTITRYNH STHYAMAVWQ
360
LGQAVALARV Q
Length:361
Mass (Da):40,256
Last modified:February 1, 1995 - v1
Checksum:i7E98F040504F75F9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18785 Genomic DNA. Translation: AAB60060.1.
U00096 Genomic DNA. Translation: AAC75743.1.
AP009048 Genomic DNA. Translation: BAA16563.1.
J02708 Genomic DNA. No translation available.
PIRiA65050.
RefSeqiNP_417181.1. NC_000913.3.
WP_001295177.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75743; AAC75743; b2701.
BAA16563; BAA16563; BAA16563.
GeneIDi947184.
KEGGiecj:JW2671.
eco:b2701.
PATRICi32120800. VBIEscCol129921_2792.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18785 Genomic DNA. Translation: AAB60060.1.
U00096 Genomic DNA. Translation: AAC75743.1.
AP009048 Genomic DNA. Translation: BAA16563.1.
J02708 Genomic DNA. No translation available.
PIRiA65050.
RefSeqiNP_417181.1. NC_000913.3.
WP_001295177.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1D0KX-ray2.02A40-361[»]
1D0LX-ray1.97A40-361[»]
1D0MX-ray2.47A40-361[»]
1LTMX-ray1.70A42-361[»]
1QDRX-ray2.10A40-361[»]
1QDTX-ray2.10A40-361[»]
1QUSX-ray1.70A40-361[»]
1QUTX-ray2.44A40-361[»]
ProteinModelPortaliP41052.
SMRiP41052. Positions 40-361.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262079. 357 interactions.
DIPiDIP-10221N.
IntActiP41052. 10 interactions.
MINTiMINT-1257117.
STRINGi511145.b2701.

Chemistry

BindingDBiP41052.
ChEMBLiCHEMBL2046262.

Protein family/group databases

CAZyiGH103. Glycoside Hydrolase Family 103.

Proteomic databases

PaxDbiP41052.
PRIDEiP41052.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75743; AAC75743; b2701.
BAA16563; BAA16563; BAA16563.
GeneIDi947184.
KEGGiecj:JW2671.
eco:b2701.
PATRICi32120800. VBIEscCol129921_2792.

Organism-specific databases

EchoBASEiEB2561.
EcoGeneiEG12699. mltB.

Phylogenomic databases

eggNOGiENOG4105CVE. Bacteria.
COG2951. LUCA.
HOGENOMiHOG000257828.
InParanoidiP41052.
KOiK08305.
OMAiSSIRDYA.
PhylomeDBiP41052.

Enzyme and pathway databases

BioCyciEcoCyc:G7410-MONOMER.
ECOL316407:JW2671-MONOMER.
MetaCyc:G7410-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP41052.
PROiP41052.

Family and domain databases

InterProiIPR023346. Lysozyme-like_dom.
IPR011757. Lytic_transglycosylase_MltB.
IPR031304. SLT_2.
[Graphical view]
PANTHERiPTHR30163. PTHR30163. 1 hit.
PfamiPF13406. SLT_2. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
TIGRFAMsiTIGR02282. MltB. 1 hit.
PROSITEiPS51257. PROKAR_LIPOPROTEIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMLTB_ECOLI
AccessioniPrimary (citable) accession number: P41052
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: September 7, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.