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Protein

Glutathione S-transferase S1

Gene

GstS1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles (PubMed:22082028). May be involved in the detoxification of metabolites produced during cellular division and morphogenesis (PubMed:1445191, PubMed:12547198).3 Publications

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.2 Publications

Kineticsi

  1. KM=0.52 mM for glutathione1 Publication
  2. KM=1.24 mM for 1-chloro-2,4-dinitrobenzene1 Publication
  1. Vmax=4.53 µmol/min/mg enzyme with 1-chloro-2,4-dinitrobenzene as substrate1 Publication
  2. Vmax=2.81 µmol/min/mg enzyme with 4-hydroxy-2-nonenal as substrate1 Publication
  3. Vmax=84.3 nmol/min/mg enzyme with adrenochrome as substrate1 Publication
  4. Vmax=0.112 µmol/min/mg enzyme with phenethyl isothiocyanate as substrate1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei54Glutathione1 Publication1
Binding sitei85Glutathione1 Publication1
Binding sitei89GlutathioneBy similarity1

GO - Molecular functioni

  • glutathione peroxidase activity Source: FlyBase
  • glutathione transferase activity Source: UniProtKB

GO - Biological processi

  • glutathione metabolic process Source: FlyBase
  • metabolic process Source: UniProtKB
  • response to oxidative stress Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Detoxification

Enzyme and pathway databases

ReactomeiR-DME-156590. Glutathione conjugation.
R-DME-2162123. Synthesis of Prostaglandins (PG) and Thromboxanes (TX).

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase S12 Publications (EC:2.5.1.182 Publications)
Alternative name(s):
GST class-sigma 1
Glutathione S-transferase 2
Gene namesi
Name:GstS1Imported
Synonyms:GST2, GSTS1-1Imported
ORF Names:CG8938Imported
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0010226. GstS1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001859171 – 249Glutathione S-transferase S1Add BLAST249

Proteomic databases

PaxDbiP41043.
PRIDEiP41043.

Expressioni

Developmental stagei

Expressed throughout development with highest levels being observed in nonfeeding stages, i.e. during embryonic and pupal development.1 Publication

Gene expression databases

BgeeiFBgn0010226.
ExpressionAtlasiP41043. baseline.
GenevisibleiP41043. DM.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi62625. 29 interactors.
DIPiDIP-21396N.
IntActiP41043. 1 interactor.
MINTiMINT-1625589.
STRINGi7227.FBpp0305731.

Structurei

Secondary structure

1249
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi50 – 58Combined sources9
Helixi59 – 61Combined sources3
Helixi62 – 71Combined sources10
Beta strandi76 – 80Combined sources5
Turni82 – 84Combined sources3
Helixi85 – 88Combined sources4
Helixi89 – 91Combined sources3
Helixi93 – 95Combined sources3
Beta strandi99 – 102Combined sources4
Beta strandi105 – 108Combined sources4
Helixi110 – 121Combined sources12
Helixi128 – 152Combined sources25
Helixi157 – 169Combined sources13
Helixi171 – 185Combined sources15
Beta strandi188 – 191Combined sources4
Helixi197 – 213Combined sources17
Turni217 – 220Combined sources4
Helixi222 – 232Combined sources11
Helixi235 – 243Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1M0UX-ray1.75A/B1-249[»]
ProteinModelPortaliP41043.
SMRiP41043.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP41043.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini48 – 125GST N-terminalAdd BLAST78
Domaini127 – 249GST C-terminalAdd BLAST123

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni96 – 97Glutathione binding2
Regioni109 – 110Glutathione binding2

Sequence similaritiesi

Belongs to the GST superfamily. Sigma family.1 Publication
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Phylogenomic databases

eggNOGiENOG410IN5I. Eukaryota.
ENOG4111I69. LUCA.
GeneTreeiENSGT00670000097856.
InParanoidiP41043.
KOiK04097.
OrthoDBiEOG091G0MBB.
PhylomeDBiP41043.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF14497. GST_C_3. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P41043-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADEAQAPPA EGAPPAEGEA PPPAEGAEGA VEGGEAAPPA EPAEPIKHSY
60 70 80 90 100
TLFYFNVKAL AEPLRYLFAY GNQEYEDVRV TRDEWPALKP TMPMGQMPVL
110 120 130 140 150
EVDGKRVHQS ISMARFLAKT VGLCGATPWE DLQIDIVVDT INDFRLKIAV
160 170 180 190 200
VSYEPEDEIK EKKLVTLNAE VIPFYLEKLE QTVKDNDGHL ALGKLTWADV
210 220 230 240
YFAGITDYMN YMVKRDLLEP YPALRGVVDA VNALEPIKAW IEKRPVTEV
Length:249
Mass (Da):27,614
Last modified:January 27, 2003 - v2
Checksum:i11AB417F550859BD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti61 – 70AEPLRYLFAY → PSPCATCSD in AAA28596 (PubMed:1445191).Curated10
Sequence conflicti79 – 85RVTRDEW → AHPRRV in AAA28596 (PubMed:1445191).Curated7
Sequence conflicti224L → V in AAA28596 (PubMed:1445191).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95198 mRNA. Translation: AAA28596.1.
AE013599 Genomic DNA. Translation: AAF57901.1.
AY118328 mRNA. Translation: AAM48357.1.
PIRiA48982.
RefSeqiNP_523767.2. NM_079043.3.
NP_725653.1. NM_166216.2.
NP_725654.1. NM_166217.3.
UniGeneiDm.19945.

Genome annotation databases

EnsemblMetazoaiFBtr0087005; FBpp0086156; FBgn0010226.
FBtr0087006; FBpp0086157; FBgn0010226.
FBtr0100258; FBpp0099646; FBgn0010226.
GeneIDi36927.
KEGGidme:Dmel_CG8938.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95198 mRNA. Translation: AAA28596.1.
AE013599 Genomic DNA. Translation: AAF57901.1.
AY118328 mRNA. Translation: AAM48357.1.
PIRiA48982.
RefSeqiNP_523767.2. NM_079043.3.
NP_725653.1. NM_166216.2.
NP_725654.1. NM_166217.3.
UniGeneiDm.19945.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1M0UX-ray1.75A/B1-249[»]
ProteinModelPortaliP41043.
SMRiP41043.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi62625. 29 interactors.
DIPiDIP-21396N.
IntActiP41043. 1 interactor.
MINTiMINT-1625589.
STRINGi7227.FBpp0305731.

Proteomic databases

PaxDbiP41043.
PRIDEiP41043.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0087005; FBpp0086156; FBgn0010226.
FBtr0087006; FBpp0086157; FBgn0010226.
FBtr0100258; FBpp0099646; FBgn0010226.
GeneIDi36927.
KEGGidme:Dmel_CG8938.

Organism-specific databases

CTDi36927.
FlyBaseiFBgn0010226. GstS1.

Phylogenomic databases

eggNOGiENOG410IN5I. Eukaryota.
ENOG4111I69. LUCA.
GeneTreeiENSGT00670000097856.
InParanoidiP41043.
KOiK04097.
OrthoDBiEOG091G0MBB.
PhylomeDBiP41043.

Enzyme and pathway databases

ReactomeiR-DME-156590. Glutathione conjugation.
R-DME-2162123. Synthesis of Prostaglandins (PG) and Thromboxanes (TX).

Miscellaneous databases

ChiTaRSiGstS1. fly.
EvolutionaryTraceiP41043.
GenomeRNAii36927.
PROiP41043.

Gene expression databases

BgeeiFBgn0010226.
ExpressionAtlasiP41043. baseline.
GenevisibleiP41043. DM.

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF14497. GST_C_3. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGSTS1_DROME
AccessioniPrimary (citable) accession number: P41043
Secondary accession number(s): Q0E945, Q9V7Y4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 27, 2003
Last modified: November 30, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.