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Protein

Putative sialic acid transporter

Gene

nanT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May be a sugar-cation symporter involved in sialic acid transport as it is required for sialic acid uptake.

GO - Molecular functioni

  • sialic acid:proton symporter activity Source: UniProtKB-HAMAP
  • sialic acid transmembrane transporter activity Source: EcoCyc
  • sugar:proton symporter activity Source: EcoCyc

GO - Biological processi

  • carboxylic acid transmembrane transport Source: GOC
  • proton transport Source: GOC
  • sialic acid transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:NANT-MONOMER.
ECOL316407:JW3193-MONOMER.
MetaCyc:NANT-MONOMER.

Protein family/group databases

TCDBi2.A.1.12.1. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Putative sialic acid transporter
Alternative name(s):
Sialic acid permease
Gene namesi
Name:nanT
Ordered Locus Names:b3224, JW3193
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG20145. nanT.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2121CytoplasmicSequence analysisAdd
BLAST
Transmembranei22 – 4221HelicalSequence analysisAdd
BLAST
Topological domaini43 – 5715PeriplasmicSequence analysisAdd
BLAST
Transmembranei58 – 7821HelicalSequence analysisAdd
BLAST
Topological domaini79 – 9113CytoplasmicSequence analysisAdd
BLAST
Transmembranei92 – 11221HelicalSequence analysisAdd
BLAST
Topological domaini113 – 14735PeriplasmicSequence analysisAdd
BLAST
Transmembranei148 – 16821HelicalSequence analysisAdd
BLAST
Topological domaini169 – 1691CytoplasmicSequence analysis
Transmembranei170 – 19021HelicalSequence analysisAdd
BLAST
Topological domaini191 – 22333PeriplasmicSequence analysisAdd
BLAST
Transmembranei224 – 24421HelicalSequence analysisAdd
BLAST
Topological domaini245 – 2462CytoplasmicSequence analysis
Transmembranei247 – 26721HelicalSequence analysisAdd
BLAST
Topological domaini268 – 27710PeriplasmicSequence analysis
Transmembranei278 – 29821HelicalSequence analysisAdd
BLAST
Topological domaini299 – 31214CytoplasmicSequence analysisAdd
BLAST
Transmembranei313 – 33321HelicalSequence analysisAdd
BLAST
Topological domaini334 – 35421PeriplasmicSequence analysisAdd
BLAST
Transmembranei355 – 37521HelicalSequence analysisAdd
BLAST
Topological domaini376 – 40530CytoplasmicSequence analysisAdd
BLAST
Transmembranei406 – 42621HelicalSequence analysisAdd
BLAST
Topological domaini427 – 4304PeriplasmicSequence analysis
Transmembranei431 – 45121HelicalSequence analysisAdd
BLAST
Topological domaini452 – 49645CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 496496Putative sialic acid transporterPRO_0000050311Add
BLAST

Proteomic databases

PaxDbiP41036.

Expressioni

Inductioni

By N-acetylneuraminate.

Interactioni

Protein-protein interaction databases

BioGridi4262439. 9 interactions.
STRINGi511145.b3224.

Structurei

3D structure databases

ProteinModelPortaliP41036.
SMRiP41036. Positions 38-218.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105JHE. Bacteria.
ENOG410XSSD. LUCA.
HOGENOMiHOG000074791.
InParanoidiP41036.
KOiK03290.
OMAiWKEKHEG.
OrthoDBiEOG6FZ4C6.
PhylomeDBiP41036.

Family and domain databases

HAMAPiMF_01238. MFS_NanT.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR004742. SA_transporter.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
TIGRFAMsiTIGR00891. 2A0112. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P41036-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTTTQNIPW YRHLNRAQWR AFSAAWLGYL LDGFDFVLIA LVLTEVQGEF
60 70 80 90 100
GLTTVQAASL ISAAFISRWF GGLMLGAMGD RYGRRLAMVT SIVLFSAGTL
110 120 130 140 150
ACGFAPGYIT MFIARLVIGM GMAGEYGSSA TYVIESWPKH LRNKASGFLI
160 170 180 190 200
SGFSVGAVVA AQVYSLVVPV WGWRALFFIG ILPIIFALWL RKNIPEAEDW
210 220 230 240 250
KEKHAGKAPV RTMVDILYRG EHRIANIVMT LAAATALWFC FAGNLQNAAI
260 270 280 290 300
VAVLGLLCAA IFISFMVQSA GKRWPTGVML MVVVLFAFLY SWPIQALLPT
310 320 330 340 350
YLKTDLAYNP HTVANVLFFS GFGAAVGCCV GGFLGDWLGT RKAYVCSLLA
360 370 380 390 400
SQLLIIPVFA IGGANVWVLG LLLFFQQMLG QGIAGILPKL IGGYFDTDQR
410 420 430 440 450
AAGLGFTYNV GALGGALAPI IGALIAQRLD LGTALASLSF SLTFVVILLI
460 470 480 490
GLDMPSRVQR WLRPEALRTH DAIDGKPFSG AVPFGSAKND LVKTKS
Length:496
Mass (Da):53,551
Last modified:November 1, 1995 - v2
Checksum:iD81DB24F7AD6A4CA
GO

Sequence cautioni

The sequence AAA58026.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAA86827.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti221 – 2211E → A in AAA86827 (PubMed:7592358).Curated
Sequence conflicti441 – 4411S → R in AAA86827 (PubMed:7592358).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19539 Genomic DNA. Translation: AAA86827.1. Different initiation.
U18997 Genomic DNA. Translation: AAA58026.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76256.2.
AP009048 Genomic DNA. Translation: BAE77267.1.
D00067 Genomic DNA. No translation available.
PIRiB65114.
RefSeqiNP_417691.4. NC_000913.3.
WP_000108454.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76256; AAC76256; b3224.
BAE77267; BAE77267; BAE77267.
GeneIDi947740.
KEGGiecj:JW3193.
eco:b3224.
PATRICi32121872. VBIEscCol129921_3320.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19539 Genomic DNA. Translation: AAA86827.1. Different initiation.
U18997 Genomic DNA. Translation: AAA58026.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76256.2.
AP009048 Genomic DNA. Translation: BAE77267.1.
D00067 Genomic DNA. No translation available.
PIRiB65114.
RefSeqiNP_417691.4. NC_000913.3.
WP_000108454.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP41036.
SMRiP41036. Positions 38-218.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262439. 9 interactions.
STRINGi511145.b3224.

Protein family/group databases

TCDBi2.A.1.12.1. the major facilitator superfamily (mfs).

Proteomic databases

PaxDbiP41036.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76256; AAC76256; b3224.
BAE77267; BAE77267; BAE77267.
GeneIDi947740.
KEGGiecj:JW3193.
eco:b3224.
PATRICi32121872. VBIEscCol129921_3320.

Organism-specific databases

EchoBASEiEB4145.
EcoGeneiEG20145. nanT.

Phylogenomic databases

eggNOGiENOG4105JHE. Bacteria.
ENOG410XSSD. LUCA.
HOGENOMiHOG000074791.
InParanoidiP41036.
KOiK03290.
OMAiWKEKHEG.
OrthoDBiEOG6FZ4C6.
PhylomeDBiP41036.

Enzyme and pathway databases

BioCyciEcoCyc:NANT-MONOMER.
ECOL316407:JW3193-MONOMER.
MetaCyc:NANT-MONOMER.

Miscellaneous databases

PROiP41036.

Family and domain databases

HAMAPiMF_01238. MFS_NanT.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR004742. SA_transporter.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
TIGRFAMsiTIGR00891. 2A0112. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Derived structure of the putative sialic acid transporter from Escherichia coli predicts a novel sugar permease domain."
    Martinez J., Steenbergen S., Vimr E.
    J. Bacteriol. 177:6005-6010(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Nucleotide sequence of the N-acetylneuraminate lyase gene of Escherichia coli."
    Kawakami B., Kudo T., Narahashi Y., Horikoshi K.
    Agric. Biol. Chem. 50:2155-2158(1986)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-50.
    Strain: JE1011.
  5. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiNANT_ECOLI
AccessioniPrimary (citable) accession number: P41036
Secondary accession number(s): P76675, Q2M8Y9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 1, 1995
Last modified: May 11, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.