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Protein

Sialic acid transporter NanT

Gene

nanT

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the proton-dependent transport of sialic acid (PubMed:22167185, PubMed:23848303). Can transport the common sialic acid N-acetylneuraminic acid (Neu5Ac) and the related sialic acids N-glycolylneuraminic acid (Neu5Gc) and 3-keto-3-deoxy-D-glycero-D-galactonononic acid (KDN) (PubMed:23848303). Functions as a bidirectional transporter in vitro (PubMed:22167185).2 Publications

GO - Molecular functioni

  • carbohydrate:proton symporter activity Source: EcoCyc
  • sialic acid transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • sialic acid transport Source: EcoCyc

Keywordsi

Biological processSugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:NANT-MONOMER
MetaCyc:NANT-MONOMER

Protein family/group databases

TCDBi2.A.1.12.1 the major facilitator superfamily (mfs)

Names & Taxonomyi

Protein namesi
Recommended name:
Sialic acid transporter NanTUniRule annotationCurated
Alternative name(s):
Sialic acid permeaseUniRule annotationCurated
Sialic acid/H(+) symporterUniRule annotationCurated
Gene namesi
Name:nanT1 PublicationUniRule annotation
Ordered Locus Names:b3224, JW3193
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG20145 nanT

Subcellular locationi

  • Cell inner membrane UniRule annotation1 Publication; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 21Cytoplasmic1 PublicationAdd BLAST21
Transmembranei22 – 42HelicalUniRule annotationAdd BLAST21
Topological domaini43 – 57Periplasmic1 PublicationAdd BLAST15
Transmembranei58 – 78HelicalUniRule annotationAdd BLAST21
Topological domaini79 – 91Cytoplasmic1 PublicationAdd BLAST13
Transmembranei92 – 112HelicalUniRule annotationAdd BLAST21
Topological domaini113 – 147Periplasmic1 PublicationAdd BLAST35
Transmembranei148 – 168HelicalUniRule annotationAdd BLAST21
Topological domaini169Cytoplasmic1 Publication1
Transmembranei170 – 190HelicalUniRule annotationAdd BLAST21
Topological domaini191 – 223Periplasmic1 PublicationAdd BLAST33
Transmembranei224 – 244HelicalUniRule annotationAdd BLAST21
Topological domaini245 – 246Cytoplasmic1 Publication2
Transmembranei247 – 267HelicalUniRule annotationAdd BLAST21
Topological domaini268 – 277Periplasmic1 Publication10
Transmembranei278 – 298HelicalUniRule annotationAdd BLAST21
Topological domaini299 – 312Cytoplasmic1 PublicationAdd BLAST14
Transmembranei313 – 333HelicalUniRule annotationAdd BLAST21
Topological domaini334 – 354Periplasmic1 PublicationAdd BLAST21
Transmembranei355 – 375HelicalUniRule annotationAdd BLAST21
Topological domaini376 – 405Cytoplasmic1 PublicationAdd BLAST30
Transmembranei406 – 426HelicalUniRule annotationAdd BLAST21
Topological domaini427 – 430Periplasmic1 Publication4
Transmembranei431 – 451HelicalUniRule annotationAdd BLAST21
Topological domaini452 – 496Cytoplasmic1 PublicationAdd BLAST45

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Deletion mutant is unable to grow in the presence of Neu5Ac, Neu5Gc or KDN.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000503111 – 496Sialic acid transporter NanTAdd BLAST496

Proteomic databases

PaxDbiP41036
PRIDEiP41036

Expressioni

Inductioni

Negatively regulated by the transcriptional repressor NanR. Induced by N-acetylneuraminate, via inactivation of NanR.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4262439, 9 interactors
STRINGi316385.ECDH10B_3401

Structurei

3D structure databases

ProteinModelPortaliP41036
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the major facilitator superfamily. Sialate:H(+) symporter (SHS) (TC 2.A.1.12) family. [View classification]UniRule annotationCurated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105JHE Bacteria
ENOG410XSSD LUCA
HOGENOMiHOG000074791
InParanoidiP41036
KOiK03290
OMAiTPHGWRP
PhylomeDBiP41036

Family and domain databases

CDDicd06174 MFS, 1 hit
HAMAPiMF_01238 MFS_NanT, 1 hit
InterProiView protein in InterPro
IPR011701 MFS
IPR020846 MFS_dom
IPR036259 MFS_trans_sf
IPR004742 SA_transporter
PfamiView protein in Pfam
PF07690 MFS_1, 1 hit
SUPFAMiSSF103473 SSF103473, 2 hits
TIGRFAMsiTIGR00891 2A0112, 1 hit
PROSITEiView protein in PROSITE
PS50850 MFS, 1 hit

Sequencei

Sequence statusi: Complete.

P41036-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTTTQNIPW YRHLNRAQWR AFSAAWLGYL LDGFDFVLIA LVLTEVQGEF
60 70 80 90 100
GLTTVQAASL ISAAFISRWF GGLMLGAMGD RYGRRLAMVT SIVLFSAGTL
110 120 130 140 150
ACGFAPGYIT MFIARLVIGM GMAGEYGSSA TYVIESWPKH LRNKASGFLI
160 170 180 190 200
SGFSVGAVVA AQVYSLVVPV WGWRALFFIG ILPIIFALWL RKNIPEAEDW
210 220 230 240 250
KEKHAGKAPV RTMVDILYRG EHRIANIVMT LAAATALWFC FAGNLQNAAI
260 270 280 290 300
VAVLGLLCAA IFISFMVQSA GKRWPTGVML MVVVLFAFLY SWPIQALLPT
310 320 330 340 350
YLKTDLAYNP HTVANVLFFS GFGAAVGCCV GGFLGDWLGT RKAYVCSLLA
360 370 380 390 400
SQLLIIPVFA IGGANVWVLG LLLFFQQMLG QGIAGILPKL IGGYFDTDQR
410 420 430 440 450
AAGLGFTYNV GALGGALAPI IGALIAQRLD LGTALASLSF SLTFVVILLI
460 470 480 490
GLDMPSRVQR WLRPEALRTH DAIDGKPFSG AVPFGSAKND LVKTKS
Length:496
Mass (Da):53,551
Last modified:November 1, 1995 - v2
Checksum:iD81DB24F7AD6A4CA
GO

Sequence cautioni

The sequence AAA58026 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAA86827 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti221E → A in AAA86827 (PubMed:7592358).Curated1
Sequence conflicti441S → R in AAA86827 (PubMed:7592358).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19539 Genomic DNA Translation: AAA86827.1 Different initiation.
U18997 Genomic DNA Translation: AAA58026.1 Different initiation.
U00096 Genomic DNA Translation: AAC76256.2
AP009048 Genomic DNA Translation: BAE77267.1
D00067 Genomic DNA No translation available.
PIRiB65114
RefSeqiNP_417691.4, NC_000913.3
WP_000108454.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76256; AAC76256; b3224
BAE77267; BAE77267; BAE77267
GeneIDi947740
KEGGiecj:JW3193
eco:b3224
PATRICifig|1411691.4.peg.3504

Similar proteinsi

Entry informationi

Entry nameiNANT_ECOLI
AccessioniPrimary (citable) accession number: P41036
Secondary accession number(s): P76675, Q2M8Y9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 1, 1995
Last modified: April 25, 2018
This is version 140 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

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