Reviewed, UniProtKB/Swiss-Prot P41027 (LEP_BACCL)

Names and origin

Protein names

Recommended name:
    Signal peptidase I
      Short name=SPase I
    EC=3.4.21.89
Alternative name(s):
    Leader peptidase I

Gene names

Name:	lepB
Synonyms:	sipC

Organism

Bacillus caldolyticus

Taxonomic identifier

1394 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	182 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.
Subcellular location	Cell membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the peptidase S26 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Hydrolase Protease
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type peptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 182	182	Signal peptidase I		PRO_0000109495
Regions
	Topological domain	1 – 13	13	Cytoplasmic Potential
	Transmembrane	14 – 30	17	Potential
	Topological domain	31 – 182	152	Extracellular Potential
Sites
	Active site	38	1	By similarity
	Active site	79	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P41027-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 908E40A6B9A1E7F9	FASTA	182	21,263
	10 20 30 40 50 60 MTKQKEKRGR RWPWFVAVCV VATLRLFVFS NYVVEGKSMM PTLESGNLLI VNKLSYDIGP 70 80 90 100 110 120 IRRFDIIVFH ANKKEDYVKR VIGLPGDRIA YKNDILYVNG KKVDEPYLRP YKQKLLDGRL 130 140 150 160 170 180 TGDFTLEEVT GKTRVPPGCI FVLGDNRLSS WDSRHFGFVK INQIVGKVDF RYWPFKQFAF QF

References

[1]

"The endogenous Bacillus subtilis (natto) plasmids pTA1015 and pTA1040 contain signal peptidase-encoding genes: identification of a new structural module on cryptic plasmids." Meijer W.J.J., de Jong A., Bea G., Wisman A., Tjalsma H., Venema G., Bron S., van Dijl J.M. Mol. Microbiol. 17:621-631(1995) [PubMed: 8801417] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z27457 Genomic DNA. Translation: CAA81813.1. L26257 Genomic DNA. Translation: AAA22759.1.
PIR	I40175.
3D structure databases
HSSP	HSSP built from PDB template 1B12 based on UniProtKB P00803.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.4.21.89. 1670.
Family and domain databases
InterPro	IPR000223. Pept_S26A_signal_pept_1. IPR019758. Pept_S26A_signal_pept_1_CS. IPR019757. Pept_S26A_signal_pept_1_Lys-AS. IPR019756. Pept_S26A_signal_pept_1_Ser-AS. IPR019759. Peptidase_S24_S26_cons-reg. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 1 hit.
Pfam	PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00727. LEADERPTASE.
TIGRFAMs	TIGR02227. sigpep_I_bact. 1 hit.
PROSITE	PS00501. SPASE_I_1. 1 hit. PS00760. SPASE_I_2. 1 hit. PS00761. SPASE_I_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEP_BACCL

Accession

Primary (citable) accession number: P41027

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	June 16, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)