P41025 (LEP2_BACAM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Signal peptidase I
Short name=SPase I
EC=3.4.21.89

Alternative name(s):
Leader peptidase I

Gene names

Name:	sipS2
Synonyms:	sip

Organism

Bacillus amyloliquefaciens (Bacillus velezensis)

Taxonomic identifier

1390 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	193 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.
Subcellular location	Cell membrane; Single-pass type II membrane protein Potential.
Sequence similarities	Belongs to the peptidase S26 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Hydrolase Protease
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type peptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 193	193	Signal peptidase I		PRO_0000109494
Regions
	Topological domain	1 – 25	25	Cytoplasmic Potential
	Transmembrane	26 – 42	17	Helical; Potential
	Topological domain	43 – 193	151	Extracellular Potential
Sites
	Active site	51	1	By similarity
	Active site	93	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P41025 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: 399D7EB879A0C7C4	FASTA	193	21,860
	10 20 30 40 50 60 MTEEQKPTSE KSVKRKSNTY WEWGKAIIIA VALALLIRHF LFEPYLVEGS SMYPTLHDGE 70 80 90 100 110 120 RLFVNKSVNY IGEIERGDIV IINGDTSKVH YVKRLIGKPG ETVEMKNDTL YINGKKIAEP 130 140 150 160 170 180 YLASNKKEAK KLGVNLTGDF GPVKVPKGKY FVMGDNRLNS MDSRNGLGLI AENRIVGTSK 190 FVFFPFHDMR QTK

References

[1]	"Bacillus amyloliquefaciens possesses a second type I signal peptidase with extensive sequence similarity to other Bacillus SPases." Hoang V., Hofemeister J. Biochim. Biophys. Acta 1269:64-68(1995) [PubMed: 7578273] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 23844 / P.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z33640 Genomic DNA. Translation: CAA83921.1.
PIR	S45022. S59966.
3D structure databases
ProteinModelPortal	P41025.
ModBase	Search...
Protein family/group databases
MEROPS	S26.004.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000223. Pept_S26A_signal_pept_1. IPR019758. Pept_S26A_signal_pept_1_CS. IPR019757. Pept_S26A_signal_pept_1_Lys-AS. IPR019756. Pept_S26A_signal_pept_1_Ser-AS. IPR019759. Peptidase_S24_S26. IPR015927. Peptidase_S24_S26A/B/C. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 2 hits.
Pfam	PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00727. LEADERPTASE.
SUPFAM	SSF51306. Pept_S24_S26_C. 1 hit.
TIGRFAMs	TIGR02227. Sigpep_I_bact. 1 hit.
PROSITE	PS00501. SPASE_I_1. 1 hit. PS00760. SPASE_I_2. 1 hit. PS00761. SPASE_I_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEP2_BACAM

Accession

Primary (citable) accession number: P41025

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 16, 2011
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program