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P41021

- URE2_SPOPA

UniProt

P41021 - URE2_SPOPA

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Protein

Urease subunit beta

Gene

ureB

Organism
Sporosarcina pasteurii (Bacillus pasteurii)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Urea + H2O = CO2 + 2 NH3.UniRule annotation

Pathwayi

GO - Molecular functioni

  1. urease activity Source: UniProtKB-EC

GO - Biological processi

  1. urea catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

UniPathwayiUPA00258; UER00370.

Names & Taxonomyi

Protein namesi
Recommended name:
Urease subunit betaUniRule annotation (EC:3.5.1.5UniRule annotation)
Alternative name(s):
Urea amidohydrolase subunit betaUniRule annotation
Gene namesi
Name:ureBUniRule annotation
OrganismiSporosarcina pasteurii (Bacillus pasteurii)
Taxonomic identifieri1474 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesPlanococcaceaeSporosarcina

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 126126Urease subunit betaPRO_0000067569Add
BLAST

Interactioni

Subunit structurei

Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme.5 PublicationsUniRule annotation

Structurei

Secondary structure

1
126
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi16 – 205
Turni21 – 244
Beta strandi27 – 348
Beta strandi36 – 383
Beta strandi40 – 434
Helixi48 – 503
Beta strandi55 – 573
Helixi59 – 624
Beta strandi65 – 673
Beta strandi74 – 774
Beta strandi82 – 898
Beta strandi104 – 1063
Helixi110 – 12011

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1IE7X-ray1.85B1-126[»]
1S3TX-ray2.10B1-126[»]
1UBPX-ray1.65B5-126[»]
2UBPX-ray2.00B5-126[»]
3UBPX-ray2.00B1-126[»]
4AC7X-ray1.50B1-126[»]
4CEUX-ray1.58B1-126[»]
4CEXX-ray1.59B1-126[»]
4UBPX-ray1.55B1-126[»]
ProteinModelPortaliP41021.
SMRiP41021. Positions 5-126.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP41021.

Family & Domainsi

Sequence similaritiesi

Belongs to the urease beta subunit family.UniRule annotation

Family and domain databases

Gene3Di2.10.150.10. 1 hit.
HAMAPiMF_01954. Urease_beta.
InterProiIPR002019. Urease_beta.
[Graphical view]
PfamiPF00699. Urease_beta. 1 hit.
[Graphical view]
SUPFAMiSSF51278. SSF51278. 1 hit.
TIGRFAMsiTIGR00192. urease_beta. 1 hit.

Sequencei

Sequence statusi: Complete.

P41021-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSNNNYIVPG EYRVAEGEIE INAGREKTTI RVSNTGDRPI QVGSHIHFVE
60 70 80 90 100
VNKELLFDRA EGIGRRLNIP SGTAARFEPG EEMEVELTEL GGNREVFGIS
110 120
DLTNGSVDNK ELILQRAKEL GYKGVE
Length:126
Mass (Da):13,959
Last modified:February 1, 1995 - v1
Checksum:iE1BE8E5209586B02
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X78411 Genomic DNA. Translation: CAA55174.1.
PIRiS47103.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X78411 Genomic DNA. Translation: CAA55174.1 .
PIRi S47103.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1IE7 X-ray 1.85 B 1-126 [» ]
1S3T X-ray 2.10 B 1-126 [» ]
1UBP X-ray 1.65 B 5-126 [» ]
2UBP X-ray 2.00 B 5-126 [» ]
3UBP X-ray 2.00 B 1-126 [» ]
4AC7 X-ray 1.50 B 1-126 [» ]
4CEU X-ray 1.58 B 1-126 [» ]
4CEX X-ray 1.59 B 1-126 [» ]
4UBP X-ray 1.55 B 1-126 [» ]
ProteinModelPortali P41021.
SMRi P41021. Positions 5-126.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi P41021.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00258 ; UER00370 .

Miscellaneous databases

EvolutionaryTracei P41021.

Family and domain databases

Gene3Di 2.10.150.10. 1 hit.
HAMAPi MF_01954. Urease_beta.
InterProi IPR002019. Urease_beta.
[Graphical view ]
Pfami PF00699. Urease_beta. 1 hit.
[Graphical view ]
SUPFAMi SSF51278. SSF51278. 1 hit.
TIGRFAMsi TIGR00192. urease_beta. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of three genes on a urease encoding DNA-fragment from Bacillus pasteurii."
    Moersdorf G., Weinmann P., Kaltwasser H.
    Submitted (JUN-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 11859 / DSM 33 / NCIB 8841 / NCTC 4822.
  2. "X-ray absorption spectroscopy study of native and phenylphosphorodiamidate-inhibited Bacillus pasteurii urease."
    Benini S., Ciurli S., Nolting H.F., Mangani S.
    Eur. J. Biochem. 239:61-66(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) OF 5-126.
  3. "Crystallization and preliminary high-resolution X-ray diffraction analysis of native and beta-mercaptoethanol-inhibited urease from Bacillus pasteurii."
    Benini S., Ciurli S., Rypniewski W.R., Wilson K.S., Mangani S.
    Acta Crystallogr. D 54:409-412(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS) IN COMPLEX WITH UREA; UREC AND BETA-MERCAPTOETHANOL.
  4. "A new proposal for urease mechanism based on the crystal structures of the native and inhibited enzyme from Bacillus pasteurii: why urea hydrolysis costs two nickels."
    Benini S., Rypniewski W.R., Wilson K.S., Miletti S., Ciurli S., Mangani S.
    Structure 7:205-216(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) IN COMPLEX WITH UREA; UREC AND DIAMIDOPHOSPHATE.
  5. "The complex of Bacillus pasteurii urease with acetohydroxamate anion from X-ray data at 1.55 A resolution."
    Benini S., Rypniewski W.R., Wilson K.S., Miletti S., Ciurli S., Mangani S.
    J. Biol. Inorg. Chem. 5:110-118(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.55 ANGSTROMS) IN COMPLEX WITH UREA; UREC AND ACETOHYDROXAMIC ACID.
  6. "Structure-based rationalization of urease inhibition by phosphate: novel insights into the enzyme mechanism."
    Benini S., Rypniewski W.R., Wilson K.S., Ciurli S., Mangani S.
    J. Biol. Inorg. Chem. 6:778-790(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) IN COMPLEX WITH UREA; UREC AND PHOSPHATE.
  7. "Molecular details of urease inhibition by boric acid: insights into the catalytic mechanism."
    Benini S., Rypniewski W.R., Wilson K.S., Mangani S., Ciurli S.
    J. Am. Chem. Soc. 126:3714-3715(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) IN COMPLEX WITH UREA; UREC AND BORATE IONS.

Entry informationi

Entry nameiURE2_SPOPA
AccessioniPrimary (citable) accession number: P41021
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: October 29, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3