Reviewed, UniProtKB/Swiss-Prot P41019 (LEU3_BACME)

Names and origin

Protein names

Recommended name:
    3-isopropylmalate dehydrogenase
    EC=1.1.1.85
Alternative name(s):
    Beta-IPM dehydrogenase
      Short name=IMDH
    3-IPM-DH

Gene names

Name:	leuB
Synonyms:	leuC

Organism

Bacillus megaterium

Taxonomic identifier

1404 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.
Catalytic activity	(2R,3S)-3-isopropylmalate + NAD(+) = 4-methyl-2-oxopentanoate + CO(2) + NADH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.
Subunit structure	Homodimer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Leucine biosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium Manganese Metal-binding NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	leucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	3-isopropylmalate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 367	367	3-isopropylmalate dehydrogenase		PRO_0000083641
Regions
	Nucleotide binding	74 – 87	14	NAD By similarity
	Nucleotide binding	278 – 290	13	NAD By similarity
Sites
	Metal binding	220	1	Magnesium or manganese By similarity
	Metal binding	244	1	Magnesium or manganese By similarity
	Metal binding	248	1	Magnesium or manganese By similarity
	Binding site	94	1	Substrate By similarity
	Binding site	104	1	Substrate By similarity
	Binding site	132	1	Substrate By similarity
	Binding site	220	1	Substrate By similarity
	Site	139	1	Important for catalysis By similarity
	Site	188	1	Important for catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	P41019-1 [UniParc]. Last modified February 1, 1995. Version 1. Checksum: DC04D48E0EEAB0DD	FASTA	367	39,942
	10 20 30 40 50 60 MTKKVAVLPG DGGQSNQRRC CRLKAIGERL RHQFEFTYGL IGGAAIDEAG SSSSRSTIET 70 80 90 100 110 120 CNEADAVLLG SVGGPKWDRF PSHLRPEKGL LAIRKELDLY ANLRPVTFYD SLADASPLKK 130 140 150 160 170 180 EYIEGVDFII VRELTGGLYF GKPSDRRTEG NKETVVDTLF YKRTEIERII ASIDTAVNRR 190 200 210 220 230 240 KKVTSVDKAN VLESSRVWRE VAEEVAKDYP DVELEHMLVD SAAMQLIRNP KYFDVVVTEN 250 260 270 280 290 300 MFGDILSDEA SMLTGSLGML PSASLTADGP SLYEPVHGSA PDIAGQNKAN PIAAAILSAA 310 320 330 340 350 360 MLLRHSFGLE KEAAVIEQAV ESVLHAGHRT ADLADGNHYL GTDKMVEVIT AVIANDSAIS SIMTASR

References

[1]	"Cloning and sequencing of the leu C and npr M genes and a putative spo IV gene from Bacillus megaterium DSM319." Meinhardt F., Busskamp M., Wittchen K.D. Appl. Microbiol. Biotechnol. 41:344-351(1994) [PubMed: 7764969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 319.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X65184 Genomic DNA. Translation: CAA46295.1.
PIR	I40226.
3D structure databases
HSSP	HSSP built from PDB template 2AYQ based on UniProtKB P12010.
ModBase	Search...
Family and domain databases
HAMAP	MF_01033. [Tree]
InterPro	IPR004429. 3-isopropylmalate_DHase. IPR001804. IsoCit_IM_DHase. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11835. IDH_IMDH_dimeric. 1 hit. PTHR11835:SF13. IPMDH. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR00169. leuB. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEU3_BACME

Accession

Primary (citable) accession number: P41019

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	February 1, 1995
Last modified:	November 4, 2008
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)