P41008 (PYRB_BACCL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aspartate carbamoyltransferase
EC=2.1.3.2

Alternative name(s):
Aspartate transcarbamylase
Short name=ATCase

Gene names

Name:

pyrB

Organism

Bacillus caldolyticus

Taxonomic identifier

1394 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate. HAMAP MF_00001
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3. HAMAP MF_00001
Subunit structure	Homotrimer.
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Molecular function	Transferase
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro aspartate carbamoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	Aspartate carbamoyltransferase HAMAP MF_00001		PRO_0000113091

Sequences

	Sequence		Length	Mass (Da)
	P41008 [UniParc]. Last modified November 1, 1995. Version 2. Checksum: FAAA34EA1620291A	FASTA	308	34,871
	10 20 30 40 50 60 MTHLFALSEL PLDEIHRLLD EAERFRSGRI WRPAAPMYVA NLFFEPSTRT KCSFEMAERK 70 80 90 100 110 120 LGLHVIPFDP ERSSVQKGET LYDTVRTLEA IGVDAVVIRH HEDAYFEALR HAVGIPIINA 130 140 150 160 170 180 GDGCGHHPTQ SLLDLLTIRQ EFGAFTGLTV AIIGDIRHSR VARSNAEVLT RLGANVLFSG 190 200 210 220 230 240 PEEWKDETNP YGTYVEVDEA IARADVVMLL RIQHERHAET MGLTKEEYHA RYGLTLERAR 250 260 270 280 290 300 RMKSGAIILH PAPVNRGVEI ASELVEAKAS RIFKQMENGV YVRMAVLKRA MEGRMEHGRM AEKWHVVQ

References

[1]	"Molecular characterization of pyrimidine biosynthesis genes from the thermophile Bacillus caldolyticus." Ghim S.Y., Nielsen P., Neuhard J. Microbiology 140:479-491(1994) [PubMed: 7516791] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 405 / NBRC 15313 / YP-T.
[2]	"The pyrimidine biosynthesis operon of the thermophile Bacillus caldolyticus includes genes for uracil phosphoribosyltransferase and uracil permease." Ghim S.Y., Neuhard J. J. Bacteriol. 176:3698-3707(1994) [PubMed: 8206848] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-61. Strain: DSM 405 / NBRC 15313 / YP-T.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X73308 Genomic DNA. Translation: CAA51736.1. X76083 Genomic DNA. Translation: CAA53698.1.
PIR	I40166.
3D structure databases
ProteinModelPortal	P41008.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_00001. Asp_carb_tr. [Tree]
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P-bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn-bd. IPR002082. Asp_carbamoyltransf_euk. [Graphical view]
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00101. ATCASE.
SUPFAM	SSF53671. Asp/Orn_carbamoyltranf. 1 hit.
TIGRFAMs	TIGR00670. Asp_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRB_BACCL

Accession

Primary (citable) accession number: P41008

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	November 1, 1995
Last modified:	September 21, 2011
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program