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Protein

Bifunctional protein PyrR

Gene

pyrR

Organism
Bacillus caldolyticus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Regulates transcriptional attenuation of the pyrimidine nucleotide (pyr) operon by binding in a uridine-dependent manner to specific sites on pyr mRNA. This disrupts an antiterminator hairpin in the RNA and favors formation of a downstream transcription terminator, leading to a reduced expression of downstream genes (Probable).Curated
Also displays a weak uracil phosphoribosyltransferase activity which is not physiologically significant.

Catalytic activityi

UMP + diphosphate = uracil + 5-phospho-alpha-D-ribose 1-diphosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei137 – 1371SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Transcription, Transcription regulation, Transcription termination

Keywords - Ligandi

RNA-binding

Protein family/group databases

MoonProtiP41007.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein PyrR
Including the following 2 domains:
Pyrimidine operon regulatory protein
Uracil phosphoribosyltransferase (EC:2.4.2.9)
Short name:
UPRTase
Gene namesi
Name:pyrR
OrganismiBacillus caldolyticus
Taxonomic identifieri1394 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillusGeobacillus thermoleovorans group

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 179179Bifunctional protein PyrRPRO_0000183028Add
BLAST

Proteomic databases

PRIDEiP41007.

Interactioni

Subunit structurei

Homodimer and homohexamer; in equilibrium.By similarity

Structurei

Secondary structure

1
179
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi2 – 76Combined sources
Helixi9 – 2719Combined sources
Beta strandi33 – 386Combined sources
Helixi39 – 5719Combined sources
Beta strandi63 – 7210Combined sources
Beta strandi82 – 909Combined sources
Beta strandi98 – 11013Combined sources
Helixi111 – 12111Combined sources
Beta strandi127 – 13610Combined sources
Beta strandi141 – 1433Combined sources
Beta strandi147 – 1526Combined sources
Beta strandi159 – 1646Combined sources
Helixi166 – 1694Combined sources
Beta strandi173 – 1786Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NONX-ray2.40A/B/C/D1-179[»]
1XZ8X-ray2.80A/B1-179[»]
1XZNX-ray2.27A/B1-179[»]
2IGBX-ray1.68A/B1-179[»]
ProteinModelPortaliP41007.
SMRiP41007. Positions 1-179.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP41007.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni40 – 412Substrate-bindingBy similarity
Regioni104 – 1129Substrate bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi100 – 11213PRPP-bindingBy similarityAdd
BLAST

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01219. PyrR.
InterProiIPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR023050. PyrR.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.

Sequencei

Sequence statusi: Complete.

P41007-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQKAVVMDEQ AIRRALTRIA HEIIERNKGI DGCVLVGIKT RGIYLARRLA
60 70 80 90 100
ERIEQIEGAS VPVGELDITL YRDDLTVKTD DHEPLVKGTN VPFPVTERNV
110 120 130 140 150
ILVDDVLFTG RTVRAAMDAV MDLGRPARIQ LAVLVDRGHR ELPIRADFVG
160 170
KNVPTSRSEL IVVELSEVDG IDQVSIHEK
Length:179
Mass (Da):19,938
Last modified:February 1, 1995 - v1
Checksum:iDF889A00B239CFB5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76083 Genomic DNA. Translation: CAA53696.1.
PIRiS38892.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76083 Genomic DNA. Translation: CAA53696.1.
PIRiS38892.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NONX-ray2.40A/B/C/D1-179[»]
1XZ8X-ray2.80A/B1-179[»]
1XZNX-ray2.27A/B1-179[»]
2IGBX-ray1.68A/B1-179[»]
ProteinModelPortaliP41007.
SMRiP41007. Positions 1-179.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MoonProtiP41007.

Proteomic databases

PRIDEiP41007.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP41007.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01219. PyrR.
InterProiIPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
IPR023050. PyrR.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The pyrimidine biosynthesis operon of the thermophile Bacillus caldolyticus includes genes for uracil phosphoribosyltransferase and uracil permease."
    Ghim S.Y., Neuhard J.
    J. Bacteriol. 176:3698-3707(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DSM 405 / NBRC 15313 / YP-T.

Entry informationi

Entry nameiPYRR_BACCL
AccessioniPrimary (citable) accession number: P41007
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: October 14, 2015
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.