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P41002 (CCNF_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 133. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cyclin-F
Alternative name(s):
F-box only protein 1
Gene names
Name:CCNF
Synonyms:FBX1, FBXO1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length786 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of CP110 during G2 phase, thereby acting as an inhibitor of centrosome reduplication. Ref.9

Subunit structure

Component of the SCF(CCNF) complex consisting of CUL1, RBX1, SKP1 and CCNF. Interacts with CCNB1; interaction is required for nuclear localization of CCNB1. Interacts with CCP110; this interaction leads to CCP110 ubiquitination and degradation via the proteasome pathway. Ref.6 Ref.7 Ref.8 Ref.9 Ref.10

Subcellular location

Nucleus. Cytoplasmcytoskeletonmicrotubule organizing centercentrosomecentriole. Note: Localization in the centrosome is rare in S phase cells and increases in G2 cells, Localizes on both the mother and daughter centrioles. Localization to centrosomes is not dependent on CP110. Also localizes to the nucleus. Ref.1 Ref.7 Ref.9

Tissue specificity

Widely expressed. Ref.1

Developmental stage

Appears in S phase, peaks in G2 phase and disappears as cells enter into mitosis (at protein level). Ref.1

Domain

The nuclear localization signals mediate the localization to the nucleus and are required for CCNB1 localization to the nucleus. Ref.7

Post-translational modification

Degraded when the spindle assembly checkpoint is activated during the G2-M transition. Degradation is not dependent on the proteasome or ubiquitin and depends on the C-terminal PEST sequence.

Phosphorylated just before cells enter into mitosis. Ref.1

Miscellaneous

Founding member of the F-box domain protein family, which obtained its name from cyclin-F (Ref.6).

Sequence similarities

Belongs to the cyclin family. Cyclin AB subfamily.

Contains 1 cyclin N-terminal domain.

Contains 1 F-box domain.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 786786Cyclin-F
PRO_0000080463

Regions

Domain29 – 7648F-box
Domain292 – 405114Cyclin N-terminal
Region582 – 766185PEST
Motif20 – 289Nuclear localization signal 1 Ref.7
Motif568 – 5747Nuclear localization signal 2 Ref.7

Experimental info

Mutagenesis35 – 362LP → AA: Impairs interaction with SKP1 and CUL1 and prevents degradation of CP110, leading to promote the formation of micronuclei.
Mutagenesis3091M → A: Abolishes ability to interact with CP110; when associated with A-352. Ref.9
Mutagenesis3521L → A: Abolishes ability to interact with CP110; when associated with A-309. Ref.9
Sequence conflict2521A → R in CAA85308. Ref.2
Sequence conflict3241K → N in CAA85308. Ref.2
Sequence conflict4341L → H in BAG36170. Ref.3
Sequence conflict6001L → V in CAA85308. Ref.2
Sequence conflict6621D → A in CAA85308. Ref.2
Sequence conflict7101P → S in AAB60342. Ref.1
Sequence conflict7311D → H in CAA85308. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P41002 [UniParc].

Last modified April 16, 2002. Version 2.
Checksum: ADB2FE41BC708898

FASTA78687,640
        10         20         30         40         50         60 
MGSGGVVHCR CAKCFCYPTK RRIRRRPRNL TILSLPEDVL FHILKWLSVE DILAVRAVHS 

        70         80         90        100        110        120 
QLKDLVDNHA SVWACASFQE LWPSPGNLKL FERAAEKGNF EAAVKLGIAY LYNEGLSVSD 

       130        140        150        160        170        180 
EARAEVNGLK ASRFFSLAER LNVGAAPFIW LFIRPPWSVS GSCCKAVVHE SLRAECQLQR 

       190        200        210        220        230        240 
THKASILHCL GRVLSLFEDE EKQQQAHDLF EEAAHQGCLT SSYLLWESDR RTDVSDPGRC 

       250        260        270        280        290        300 
LHSFRKLRDY AAKGCWEAQL SLAKACANAN QLGLEVRASS EIVCQLFQAS QAVSKQQVFS 

       310        320        330        340        350        360 
VQKGLNDTMR YILIDWLVEV ATMKDFTSLC LHLTVECVDR YLRRRLVPRY RLQLLGIACM 

       370        380        390        400        410        420 
VICTRFISKE ILTIREAVWL TDNTYKYEDL VRMMGEIVSA LEGKIRVPTV VDYKEVLLTL 

       430        440        450        460        470        480 
VPVELRTQHL CSFLCELSLL HTSLSAYAPA RLAAAALLLA RLTHGQTQPW TTQLWDLTGF 

       490        500        510        520        530        540 
SYEDLIPCVL SLHKKCFHDD APKDYRQVSL TAVKQRFEDK RYGEISQEEV LSYSQLCAAL 

       550        560        570        580        590        600 
GVTQDSPDPP TFLSTGEIHA FLSSPSGRRT KRKRENSLQE DRGSFVTTPT AELSSQEETL 

       610        620        630        640        650        660 
LGSFLDWSLD CCSGYEGDQE SEGEKEGDVT APSGILDVTV VYLNPEQHCC QESSDEEACP 

       670        680        690        700        710        720 
EDKGPQDPQA LALDTQIPAT PGPKPLVRTS REPGKDVTTS GYSSVSTASP TSSVDGGLGA 

       730        740        750        760        770        780 
LPQPTSVLSL DSDSHTQPCH HQARKSCLQC RPPSPPESSV PQQQVKRINL CIHSEEEDMN 


LGLVRL 

« Hide

References

« Hide 'large scale' references
[1]"Human cyclin F."
Bai C., Richman R., Elledge S.J.
EMBO J. 13:6087-6098(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION, PHOSPHORYLATION.
[2]"A novel cyclin gene (CCNF) in the region of the polycystic kidney disease gene (PKD1)."
Kraus B., Pohlschmidt M., Leung A.L.S., Germino G.G., Snarey A., Schneider M.C., Reeders S.T., Frischauf A.-M.
Genomics 24:27-33(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Thymus.
[4]"The sequence and analysis of duplication-rich human chromosome 16."
Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J. expand/collapse author list , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A.
Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Eye.
[6]"SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box."
Bai C., Sen P., Hofmann K., Ma L., Goebl M., Harper J.W., Elledge S.J.
Cell 86:263-274(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SKP1.
[7]"Cyclin F regulates the nuclear localization of cyclin B1 through a cyclin-cyclin interaction."
Kong M., Barnes E.A., Ollendorff V., Donoghue D.J.
EMBO J. 19:1378-1388(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, NUCLEAR LOCALIZATION SIGNAL, DOMAIN, INTERACTION WITH CCNB1.
[8]"Cyclin F is degraded during G2-M by mechanisms fundamentally different from other cyclins."
Fung T.K., Siu W.Y., Yam C.H., Lau A., Poon R.Y.
J. Biol. Chem. 277:35140-35149(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SKP1, DEGRADATION.
[9]"SCF(Cyclin F) controls centrosome homeostasis and mitotic fidelity through CP110 degradation."
D'Angiolella V., Donato V., Vijayakumar S., Saraf A., Florens L., Washburn M.P., Dynlacht B., Pagano M.
Nature 466:138-142(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, IDENTIFICATION IN THE SCF(CCNF) COMPLEX WITH SKP1 AND CUL1, INTERACTION WITH CP110, MUTAGENESIS OF 35-LEU-PRO-36; MET-309 AND LEU-352.
[10]"Neurl4, a novel daughter centriole protein, prevents formation of ectopic microtubule organizing centres."
Li J., Kim S., Kobayashi T., Liang F.X., Korzeniewski N., Duensing S., Dynlacht B.D.
EMBO Rep. 13:547-553(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CCP110.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U17105 mRNA. Translation: AAB60342.1.
Z36714 mRNA. Translation: CAA85308.1.
AK313371 mRNA. Translation: BAG36170.1.
AC106820 Genomic DNA. No translation available.
BC012349 mRNA. Translation: AAH12349.1.
PIRA55501.
RefSeqNP_001752.2. NM_001761.2.
UniGeneHs.1973.

3D structure databases

ProteinModelPortalP41002.
SMRP41002. Positions 88-140, 286-532.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid107339. 11 interactions.
DIPDIP-44939N.
IntActP41002. 6 interactions.
MINTMINT-1543357.
STRING9606.ENSP00000380256.

PTM databases

PhosphoSiteP41002.

Polymorphism databases

DMDM20178283.

Proteomic databases

PaxDbP41002.
PRIDEP41002.

Protocols and materials databases

DNASU899.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000397066; ENSP00000380256; ENSG00000162063.
GeneID899.
KEGGhsa:899.
UCSCuc002cqd.1. human.

Organism-specific databases

CTD899.
GeneCardsGC16P002479.
HGNCHGNC:1591. CCNF.
MIM600227. gene.
neXtProtNX_P41002.
PharmGKBPA26156.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG283043.
HOGENOMHOG000285971.
HOVERGENHBG050838.
InParanoidP41002.
KOK10289.
OMAKSCLQCR.
OrthoDBEOG7WDN1V.
PhylomeDBP41002.
TreeFamTF101006.

Enzyme and pathway databases

SignaLinkP41002.

Gene expression databases

ArrayExpressP41002.
BgeeP41002.
CleanExHS_CCNF.
GenevestigatorP41002.

Family and domain databases

Gene3D1.10.472.10. 2 hits.
1.25.40.10. 2 hits.
InterProIPR028857. CCNF_metazoan.
IPR013763. Cyclin-like.
IPR004367. Cyclin_C-dom.
IPR006671. Cyclin_N.
IPR001810. F-box_dom.
IPR011990. TPR-like_helical.
[Graphical view]
PANTHERPTHR10177:SF18. PTHR10177:SF18. 1 hit.
PfamPF02984. Cyclin_C. 1 hit.
PF00134. Cyclin_N. 1 hit.
PF00646. F-box. 1 hit.
[Graphical view]
SMARTSM00385. CYCLIN. 2 hits.
SM00256. FBOX. 1 hit.
[Graphical view]
SUPFAMSSF47954. SSF47954. 2 hits.
SSF81383. SSF81383. 1 hit.
PROSITEPS00292. CYCLINS. 1 hit.
PS50181. FBOX. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiCCNF.
GenomeRNAi899.
NextBio3714.
PROP41002.
SOURCESearch...

Entry information

Entry nameCCNF_HUMAN
AccessionPrimary (citable) accession number: P41002
Secondary accession number(s): B2R8H3, Q96EG9
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: April 16, 2002
Last modified: March 19, 2014
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 16

Human chromosome 16: entries, gene names and cross-references to MIM