Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

tRNA (cytosine(38)-C(5))-methyltransferase

Gene

pmt1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Specifically methylates cytosine 38 in the anticodon loop of tRNA(Asp). Can also methylate cytosine 38 in tRNA(Glu), albeit to a lower level, but not tRNA(Lys). Pmt1-dependent tRNA methylation is induced by nitrogen limitation and depends on the nutrient-sensing protein kinase sck2. Does not have DNA-methylation activity.1 Publication

Caution

Was originally thought not to have cytosine-5 methyltransferase activity, and this was attributed to the insertion of a Ser residue between the Pro-Cys motif found at the active site of C5 MTases (PubMed:8636983). When this serine is deleted it becomes catalytically active and recognizes and methylates the sequence CC[AT]GG. This was in agreement with S.pombe lacking m5C DNA-methylation. However, it has later been shown that it has tRNA-methyltransferase activity despite this sequence variation (PubMed:23074192).2 Publications

Catalytic activityi

S-adenosyl-L-methionine + cytosine(38) in tRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(38) in tRNA.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei81PROSITE-ProRule annotation1

GO - Molecular functioni

  • tRNA (cytosine) methyltransferase activity Source: PomBase
  • tRNA (cytosine-5-)-methyltransferase activity Source: PomBase
  • tRNA binding Source: PomBase

GO - Biological processi

  • tRNA C5-cytosine methylation Source: PomBase

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (cytosine(38)-C(5))-methyltransferase (EC:2.1.1.204)
Alternative name(s):
DNA (cytosine-5)-methyltransferase-like protein 2
Short name:
Dnmt2
M.SpomI
SpIM.SpoI
Gene namesi
Name:pmt1
ORF Names:SPBC19C2.02
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC19C2.02
PomBaseiSPBC19C2.02 pmt1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi81C → A: Loss of methyltransferase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000880421 – 330tRNA (cytosine(38)-C(5))-methyltransferaseAdd BLAST330

Proteomic databases

MaxQBiP40999
PaxDbiP40999
PRIDEiP40999

Expressioni

Inductioni

Constitutively expressed at protein and RNA levels.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi277286, 16 interactors
STRINGi4896.SPBC19C2.02.1

Structurei

3D structure databases

ProteinModelPortaliP40999
SMRiP40999
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 330SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd BLAST324

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000265038
InParanoidiP40999
KOiK15336
OMAiLRYFTPK
OrthoDBiEOG092C4OMC
PhylomeDBiP40999

Family and domain databases

InterProiView protein in InterPro
IPR001525 C5_MeTfrase
IPR031303 C5_meth_CS
IPR029063 SAM-dependent_MTases
PfamiView protein in Pfam
PF00145 DNA_methylase, 1 hit
PRINTSiPR00105 C5METTRFRASE
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00675 dcm, 1 hit
PROSITEiView protein in PROSITE
PS00095 C5_MTASE_2, 1 hit
PS51679 SAM_MT_C5, 1 hit

Sequencei

Sequence statusi: Complete.

P40999-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLSTKRLRVL ELYSGIGGMH YALNLANIPA DIVCAIDINP QANEIYNLNH
60 70 80 90 100
GKLAKHMDIS TLTAKDFDAF DCKLWTMSPS CQPFTRIGNR KDILDPRSQA
110 120 130 140 150
FLNILNVLPH VNNLPEYILI ENVQGFEESK AAEECRKVLR NCGYNLIEGI
160 170 180 190 200
LSPNQFNIPN SRSRWYGLAR LNFKGEWSID DVFQFSEVAQ KEGEVKRIRD
210 220 230 240 250
YLEIERDWSS YMVLESVLNK WGHQFDIVKP DSSSCCCFTR GYTHLVQGAG
260 270 280 290 300
SILQMSDHEN THEQFERNRM ALQLRYFTAR EVARLMGFPE SLEWSKSNVT
310 320 330
EKCMYRLLGN SINVKVVSYL ISLLLEPLNF
Length:330
Mass (Da):37,976
Last modified:February 1, 1995 - v1
Checksum:i50A7121FA7CF58A1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82444 Genomic DNA Translation: CAA57824.1
CU329671 Genomic DNA Translation: CAB52029.1
PIRiS53990
RefSeqiNP_595687.1, NM_001021584.2

Genome annotation databases

EnsemblFungiiSPBC19C2.02.1; SPBC19C2.02.1:pep; SPBC19C2.02
GeneIDi2540766
KEGGispo:SPBC19C2.02

Similar proteinsi

Entry informationi

Entry nameiPMT1M_SCHPO
AccessioniPrimary (citable) accession number: P40999
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: February 1, 1995
Last modified: March 28, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health