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Protein

Thiamine thiazole synthase

Gene

thi2

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Involved in biosynthesis of the thiamine precursor thiazole. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid (ADT), an adenylated thiazole intermediate. The reaction includes an iron-dependent sulfide transfer from a conserved cysteine residue of the protein to a thiazole intermediate. The enzyme can only undergo a single turnover, which suggests it is a suicide enzyme. May have additional roles in adaptation to various stress conditions and in DNA damage tolerance.UniRule annotation

Cofactori

Fe cationUniRule annotationNote: Binds 1 Fe cation per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei87Substrate; via amide nitrogenUniRule annotation1
Binding sitei116Substrate; via amide nitrogenUniRule annotation1
Binding sitei181Substrate; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei217SubstrateUniRule annotation1
Binding sitei232SubstrateUniRule annotation1
Binding sitei284Substrate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

  • response to stress Source: InterPro
  • thiamine biosynthetic process Source: PomBase
  • thiazole biosynthetic process Source: PomBase

Keywordsi

Biological processThiamine biosynthesis
LigandIron, Metal-binding, NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine thiazole synthaseUniRule annotation
Alternative name(s):
Thiazole biosynthetic enzymeUniRule annotation
Gene namesi
Name:thi2
Synonyms:nmt2
ORF Names:SPBC26H8.01
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC26H8.01
PomBaseiSPBC26H8.01 thi2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000340561 – 328Thiamine thiazole synthaseAdd BLAST328

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2152,3-didehydroalanine (Cys)UniRule annotation1

Post-translational modificationi

During the catalytic reaction, a sulfide is transferred from Cys-215 to a reaction intermediate, generating a dehydroalanine residue.UniRule annotation

Proteomic databases

PaxDbiP40998
PRIDEiP40998

PTM databases

iPTMnetiP40998

Expressioni

Inductioni

Repressed by thiamine.1 Publication

Interactioni

Subunit structurei

Homooctamer.UniRule annotation

Protein-protein interaction databases

BioGridi277054, 7 interactors
STRINGi4896.SPBC26H8.01.1

Structurei

3D structure databases

ProteinModelPortaliP40998
SMRiP40998
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni108 – 109Substrate bindingUniRule annotation2
Regioni294 – 296Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the THI4 family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000106048
InParanoidiP40998
KOiK03146
OMAiMWGGGMM
OrthoDBiEOG092C3CAV
PhylomeDBiP40998

Family and domain databases

Gene3Di3.50.50.60, 1 hit
HAMAPiMF_03158 THI4, 1 hit
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR027495 Sti35
IPR002922 Thi4_fam
SUPFAMiSSF51905 SSF51905, 1 hit
TIGRFAMsiTIGR00292 TIGR00292, 1 hit

Sequencei

Sequence statusi: Complete.

P40998-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPATAVVTP QTAFKTDLPV EKTAHNTVVK SEMGALSKAY PTYSLDESFS
60 70 80 90 100
FAPIRESTVS RAMTRRYFSD LDKYAESDIV IVGAGSAGLT AAYYIGTRRP
110 120 130 140 150
DLKIAIIEAS VAPGGGAWLG GQLFSAMVVR KPADLFLNEI GVPYEDEGDY
160 170 180 190 200
VVVKHAALFT STVMARTLAL PNVKLFNATA VEDLIVKEGK DGKQRIAGVV
210 220 230 240 250
TNWTLVSLNH GLQSCMDPNT INAHLVVSAT GHDGPFGAFC VKRLASAQLV
260 270 280 290 300
SNLHDMRPLD MNRAEDLIVK GTREVFPGMI VGGMELSEFD GANRMGPTFG
310 320
GMMFSGIKAA QEALAIFDER KAVNEKYL
Length:328
Mass (Da):35,274
Last modified:January 11, 2001 - v2
Checksum:iBF18CCB367BEC421
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti299 – 328FGGMM…NEKYL → SVV in CAA57779 (PubMed:7992507).CuratedAdd BLAST30

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X82363 Genomic DNA Translation: CAA57779.1
CU329671 Genomic DNA Translation: CAA21093.1
PIRiT40013
RefSeqiNP_596642.1, NM_001022564.2

Genome annotation databases

EnsemblFungiiSPBC26H8.01.1; SPBC26H8.01.1:pep; SPBC26H8.01
GeneIDi2540526
KEGGispo:SPBC26H8.01

Similar proteinsi

Entry informationi

Entry nameiTHI4_SCHPO
AccessioniPrimary (citable) accession number: P40998
Secondary accession number(s): O74785
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: January 11, 2001
Last modified: March 28, 2018
This is version 126 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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