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Protein

1,3-beta-glucan synthase component GSC2

Gene

GSC2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Alternate catalytic subunit of the 1,3-beta-glucan synthase (GS). Synthesizes 1,3-beta-glucan, a major structural component of the yeast cell wall. Required for spore wall assembly. Negative regulation of activity by SMK1 is important for spore wall deposition. Activity is positively regulated by RHO1.2 Publications

Catalytic activityi

UDP-glucose + ((1->3)-beta-D-glucosyl)(n) = UDP + ((1->3)-beta-D-glucosyl)(n+1).2 Publications

GO - Molecular functioni

  • 1,3-beta-D-glucan synthase activity Source: SGD

GO - Biological processi

  • (1->3)-beta-D-glucan biosynthetic process Source: SGD
  • ascospore wall assembly Source: SGD
  • regulation of cell shape Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Cell shape, Cell wall biogenesis/degradation, Sporulation

Enzyme and pathway databases

BioCyciYEAST:YGR032W-MONOMER.

Protein family/group databases

CAZyiGT48. Glycosyltransferase Family 48.

Names & Taxonomyi

Protein namesi
Recommended name:
1,3-beta-glucan synthase component GSC2 (EC:2.4.1.34)
Alternative name(s):
1,3-beta-D-glucan-UDP glucosyltransferase
FK506 sensitivity protein 2
Glucan synthase of cerevisiae protein 2
Gene namesi
Name:GSC2
Synonyms:FKS2, GLS2
Ordered Locus Names:YGR032W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR032W.
SGDiS000003264. GSC2.

Subcellular locationi

  • Membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 473473ExtracellularSequence analysisAdd
BLAST
Transmembranei474 – 49421HelicalSequence analysisAdd
BLAST
Topological domaini495 – 51117CytoplasmicSequence analysisAdd
BLAST
Transmembranei512 – 53221HelicalSequence analysisAdd
BLAST
Topological domaini533 – 55018ExtracellularSequence analysisAdd
BLAST
Transmembranei551 – 57121HelicalSequence analysisAdd
BLAST
Topological domaini572 – 58211CytoplasmicSequence analysisAdd
BLAST
Transmembranei583 – 60321HelicalSequence analysisAdd
BLAST
Topological domaini604 – 1579976ExtracellularSequence analysisAdd
BLAST
Transmembranei1580 – 160021HelicalSequence analysisAdd
BLAST
Topological domaini1601 – 162020CytoplasmicSequence analysisAdd
BLAST
Transmembranei1621 – 164121HelicalSequence analysisAdd
BLAST
Topological domaini1642 – 1758117ExtracellularSequence analysisAdd
BLAST
Transmembranei1759 – 177921HelicalSequence analysisAdd
BLAST
Topological domaini1780 – 182142CytoplasmicSequence analysisAdd
BLAST
Transmembranei1822 – 184221HelicalSequence analysisAdd
BLAST
Topological domaini1843 – 189553ExtracellularSequence analysisAdd
BLAST

GO - Cellular componenti

  • 1,3-beta-D-glucan synthase complex Source: SGD
  • integral component of membrane Source: UniProtKB-KW
  • prospore membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL3134.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 189518951,3-beta-glucan synthase component GSC2PRO_0000121726Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki278 – 278Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei288 – 2881PhosphothreonineBy similarity
Modified residuei291 – 2911PhosphothreonineBy similarity
Cross-linki405 – 405Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki806 – 806Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki929 – 929Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki934 – 934Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki1558 – 1558Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki1566 – 1566Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP40989.
TopDownProteomicsiP40989.

PTM databases

iPTMnetiP40989.

Expressioni

Inductioni

Under starvation and during sporulation. Also by pheromones and calcium in a calcineurin-dependent manner.1 Publication

Interactioni

Subunit structurei

Component of the 1,3-beta-glucan synthase (GS), composed of two alternate catalytic subunits FKS1 or GSC2, and a regulatory subunit RHO1. Interacts with SMK1.1 Publication

Protein-protein interaction databases

BioGridi33277. 42 interactions.
DIPiDIP-2511N.
IntActiP40989. 6 interactions.
MINTiMINT-1326074.

Chemistry

BindingDBiP40989.

Structurei

3D structure databases

ProteinModelPortaliP40989.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 48 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000004828.
HOGENOMiHOG000216604.
InParanoidiP40989.
KOiK00706.
OMAiWKKCFFK.
OrthoDBiEOG092C025F.

Family and domain databases

InterProiIPR026899. FKS1-like_dom1.
IPR003440. Glyco_trans_48.
[Graphical view]
PfamiPF14288. FKS1_dom1. 1 hit.
PF02364. Glucan_synthase. 1 hit.
[Graphical view]
SMARTiSM01205. FKS1_dom1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40989-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYNDPNLNG QYYSNGDGTG DGNYPTYQVT QDQSAYDEYG QPIYTQNQLD
60 70 80 90 100
DGYYDPNEQY VDGTQFPQGQ DPSQDQGPYN NDASYYNQPP NMMNPSSQDG
110 120 130 140 150
ENFSDFSSYG PPSGTYPNDQ YTPSQMSYPD QDGSSGASTP YGNGVVNGNG
160 170 180 190 200
QYYDPNAIEM ALPNDPYPAW TADPQSPLPI EQIEDIFIDL TNKFGFQRDS
210 220 230 240 250
MRNMFDHFMT LLDSRSSRMS PEQALLSLHA DYIGGDTANY KKWYFAAQLD
260 270 280 290 300
MDDEIGFRNM KLGKLSRKAR KAKKKNKKAM QEASPEDTEE TLNQIEGDNS
310 320 330 340 350
LEAADFRWKS KMNQLSPFEM VRQIALFLLC WGEANQVRFT PECLCFIYKC
360 370 380 390 400
ASDYLDSAQC QQRPDPLPEG DFLNRVITPL YRFIRSQVYE IVDGRYVKSE
410 420 430 440 450
KDHNKVIGYD DVNQLFWYPE GIAKIVMEDG TRLIDLPAEE RYLKLGEIPW
460 470 480 490 500
DDVFFKTYKE TRSWLHLVTN FNRIWIMHIS VYWMYCAYNA PTFYTHNYQQ
510 520 530 540 550
LVDNQPLAAY KWATAALGGT VASLIQVAAT LCEWSFVPRK WAGAQHLSRR
560 570 580 590 600
FWFLCVIMGI NLGPVIFVFA YDKDTVYSTA AHVVGAVMFF VAVATLVFFS
610 620 630 640 650
VMPLGGLFTS YMKKSTRSYV ASQTFTASFA PLHGLDRWMS YLVWVTVFAA
660 670 680 690 700
KYAESYFFLI LSLRDPIRIL STTSMRCTGE YWWGNKICKV QPKIVLGLMI
710 720 730 740 750
ATDFILFFLD TYLWYIVVNT VFSVGKSFYL GISILTPWRN IFTRLPKRIY
760 770 780 790 800
SKILATTDME IKYKPKVLIS QIWNAIIISM YREHLLAIDH VQKLLYHQVP
810 820 830 840 850
SEIEGKRTLR APTFFVSQDD NNFETEFFPR DSEAERRISF FAQSLSTPIP
860 870 880 890 900
EPLPVDNMPT FTVLTPHYAE RILLSLREII REDDQFSRVT LLEYLKQLHP
910 920 930 940 950
VEWDCFVKDT KILAEETAAY ENNEDEPEKE DALKSQIDDL PFYCIGFKSA
960 970 980 990 1000
APEYTLRTRI WASLRSQTLY RTISGFMNYS RAIKLLYRVE NPEIVQMFGG
1010 1020 1030 1040 1050
NADGLERELE KMARRKFKFL VSMQRLAKFK PHELENAEFL LRAYPDLQIA
1060 1070 1080 1090 1100
YLDEEPPLNE GEEPRIYSAL IDGHCEILEN GRRRPKFRVQ LSGNPILGDG
1110 1120 1130 1140 1150
KSDNQNHALI FYRGEYIQLI DANQDNYLEE CLKIRSVLAE FEELGIEQIH
1160 1170 1180 1190 1200
PYTPGLKYED QSTNHPVAIV GAREYIFSEN SGVLGDVAAG KEQTFGTLFA
1210 1220 1230 1240 1250
RTLAQIGGKL HYGHPDFINA TFMTTRGGVS KAQKGLHLNE DIYAGMNAVL
1260 1270 1280 1290 1300
RGGRIKHCEY YQCGKGRDLG FGTILNFTTK IGAGMGEQML SREYYYLGTQ
1310 1320 1330 1340 1350
LPIDRFLTFY YAHPGFHLNN LFIQLSLQMF MLTLVNLHAL AHESILCVYD
1360 1370 1380 1390 1400
RDKPITDVLY PIGCYNFHPA IDWVRRYTLS IFIVFWIAFV PIVVQELIER
1410 1420 1430 1440 1450
GLWKATQRFF RHILSLSPMF EVFAGQIYSS ALLSDIAVGG ARYISTGRGF
1460 1470 1480 1490 1500
ATSRIPFSIL YSRFAGSAIY MGSRSMLMLL FGTVAHWQAP LLWFWASLSA
1510 1520 1530 1540 1550
LIFAPFIFNP HQFAWEDFFL DYRDYIRWLS RGNNKYHRNS WIGYVRMSRS
1560 1570 1580 1590 1600
RVTGFKRKLV GDESEKSAGD ASRAHRTNLI MAEIIPCAIY AAGCFIAFTF
1610 1620 1630 1640 1650
INAQTGVKTT DEDRVNSTLR IIICTLAPIV IDIGVLFFCM GLSCCSGPLL
1660 1670 1680 1690 1700
GMCCKKTGSV MAGIAHGIAV VVHIVFFIVM WVLEGFSFVR MLIGVVTCIQ
1710 1720 1730 1740 1750
CQRLIFHCMT VLLLTREFKN DHANTAFWTG KWYSTGLGYM AWTQPTRELT
1760 1770 1780 1790 1800
AKVIELSEFA ADFVLGHVIL IFQLPVICIP KIDKFHSIML FWLKPSRQIR
1810 1820 1830 1840 1850
PPIYSLKQAR LRKRMVRRYC SLYFLVLIIF AGCIVGPAVA SAHVPKDLGS
1860 1870 1880 1890
GLTGTFHNLV QPRNVSNNDT GSQMSTYKSH YYTHTPSLKT WSTIK
Length:1,895
Mass (Da):216,990
Last modified:October 1, 1996 - v2
Checksum:i9DDE4D8C19BC24DA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti137 – 1371A → T in BAA07707 (PubMed:7649185).Curated
Sequence conflicti261 – 2611K → E in BAA07707 (PubMed:7649185).Curated
Sequence conflicti275 – 2751K → R in BAA07707 (PubMed:7649185).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti1059 – 10591N → S in strain: SK1. 1 Publication
Natural varianti1853 – 18542TG → KD in strain: SK1.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D42127 Genomic DNA. Translation: BAA07707.1.
U16783 Genomic DNA. Translation: AAA85676.1.
DQ115390 Genomic DNA. Translation: AAZ22447.1.
Z72817 Genomic DNA. Translation: CAA97020.1.
BK006941 Genomic DNA. Translation: DAA08129.1.
PIRiS50240.
RefSeqiNP_011546.3. NM_001181161.3.

Genome annotation databases

EnsemblFungiiYGR032W; YGR032W; YGR032W.
GeneIDi852920.
KEGGisce:YGR032W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D42127 Genomic DNA. Translation: BAA07707.1.
U16783 Genomic DNA. Translation: AAA85676.1.
DQ115390 Genomic DNA. Translation: AAZ22447.1.
Z72817 Genomic DNA. Translation: CAA97020.1.
BK006941 Genomic DNA. Translation: DAA08129.1.
PIRiS50240.
RefSeqiNP_011546.3. NM_001181161.3.

3D structure databases

ProteinModelPortaliP40989.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33277. 42 interactions.
DIPiDIP-2511N.
IntActiP40989. 6 interactions.
MINTiMINT-1326074.

Chemistry

BindingDBiP40989.
ChEMBLiCHEMBL3134.

Protein family/group databases

CAZyiGT48. Glycosyltransferase Family 48.

PTM databases

iPTMnetiP40989.

Proteomic databases

MaxQBiP40989.
TopDownProteomicsiP40989.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR032W; YGR032W; YGR032W.
GeneIDi852920.
KEGGisce:YGR032W.

Organism-specific databases

EuPathDBiFungiDB:YGR032W.
SGDiS000003264. GSC2.

Phylogenomic databases

GeneTreeiENSGT00390000004828.
HOGENOMiHOG000216604.
InParanoidiP40989.
KOiK00706.
OMAiWKKCFFK.
OrthoDBiEOG092C025F.

Enzyme and pathway databases

BioCyciYEAST:YGR032W-MONOMER.

Miscellaneous databases

PROiP40989.

Family and domain databases

InterProiIPR026899. FKS1-like_dom1.
IPR003440. Glyco_trans_48.
[Graphical view]
PfamiPF14288. FKS1_dom1. 1 hit.
PF02364. Glucan_synthase. 1 hit.
[Graphical view]
SMARTiSM01205. FKS1_dom1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFKS2_YEAST
AccessioniPrimary (citable) accession number: P40989
Secondary accession number(s): D6VUG8, Q45U52
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: October 1, 1996
Last modified: September 7, 2016
This is version 141 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Deletion leads to caspofungin resistance.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.