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Protein

Low-affinity Fe(2+) transport protein

Gene

FET4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for Fe2+ ion low affinity uptake.

GO - Molecular functioni

  • copper ion transmembrane transporter activity Source: SGD
  • iron ion transmembrane transporter activity Source: SGD

GO - Biological processi

  • copper ion import Source: SGD
  • copper ion transmembrane transport Source: GOC
  • intracellular copper ion transport Source: SGD
  • iron ion homeostasis Source: UniProtKB-KW
  • low-affinity iron ion transmembrane transport Source: SGD
  • zinc II ion transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Ion transport, Iron transport, Transport

Keywords - Ligandi

Iron

Enzyme and pathway databases

BioCyciYEAST:G3O-32983-MONOMER.

Protein family/group databases

TCDBi9.A.9.1.1. the low affinity fe(2+) transporter (fet) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Low-affinity Fe(2+) transport protein
Alternative name(s):
Low-affinity Fe(II) transport protein
Gene namesi
Name:FET4
Ordered Locus Names:YMR319C
ORF Names:YM9924.11C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR319C.
SGDiS000004938. FET4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 9797ExtracellularSequence analysisAdd
BLAST
Transmembranei98 – 11821HelicalSequence analysisAdd
BLAST
Topological domaini119 – 225107CytoplasmicSequence analysisAdd
BLAST
Transmembranei226 – 24621HelicalSequence analysisAdd
BLAST
Topological domaini247 – 27125ExtracellularSequence analysisAdd
BLAST
Transmembranei272 – 29221HelicalSequence analysisAdd
BLAST
Topological domaini293 – 35462CytoplasmicSequence analysisAdd
BLAST
Transmembranei355 – 37521HelicalSequence analysisAdd
BLAST
Topological domaini376 – 3838ExtracellularSequence analysis
Transmembranei384 – 40421HelicalSequence analysisAdd
BLAST
Topological domaini405 – 46561CytoplasmicSequence analysisAdd
BLAST
Transmembranei466 – 48621HelicalSequence analysisAdd
BLAST
Topological domaini487 – 4937ExtracellularSequence analysis
Transmembranei494 – 51421HelicalSequence analysisAdd
BLAST
Topological domaini515 – 55238CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: SGD
  • intracellular Source: GOC
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 552552Low-affinity Fe(2+) transport proteinPRO_0000087233Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei48 – 481PhosphoserineCombined sources
Modified residuei50 – 501PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP40988.
PeptideAtlasiP40988.

PTM databases

iPTMnetiP40988.

Expressioni

Inductioni

By iron deprivation.

Interactioni

Protein-protein interaction databases

BioGridi35499. 48 interactions.
DIPiDIP-5543N.
IntActiP40988. 1 interaction.
MINTiMINT-480025.

Structurei

3D structure databases

ProteinModelPortaliP40988.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the FET4 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000111194.
InParanoidiP40988.
KOiK19792.
OMAiNWWLIIG.
OrthoDBiEOG7S2290.

Family and domain databases

InterProiIPR007251. Iron_permease_Fet4.
[Graphical view]
PfamiPF04120. Iron_permease. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40988-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKIAEFLGN PGARPDVHHR APTVDCKQYE EFGDSNDYKN DDVVRVVSHS
60 70 80 90 100
DESTDDELCN VNLTETGAIF TSKGFTGLSK GFTDKTLDFL VRVAGSQAVF
110 120 130 140 150
FIVWIILIIW VVIGIVYNAP FNWQVVMQDG QSIQSYVWDT LLMRQQLMST
160 170 180 190 200
HEQILICGRL KSRLASFKNY LTRSTPEEEK ADCTVEANEV SSVENHIDPS
210 220 230 240 250
AINGELPVEN WYDRLSNVAS RYMGSIAAMV IFWIGIFVWI GCGAIPKDAG
260 270 280 290 300
NTPPYTGETT GSNPRLKKFS DAWQMYINTA VAVSLLICTT FLQNIRARHD
310 320 330 340 350
YFTGRFLVDI FDMDEKIDYR IRKHFNDFET PHPVVTIESK KRSTGRKMID
360 370 380 390 400
WYADIIGTGI GVLIGVAVFA TWIGIGSPMK WDDNWWLIIG TYTGLIGFLD
410 420 430 440 450
GFVLREVYFR IVQHEEKNYS DVAKEDLELF QELGIECPEE FSGKAPEINT
460 470 480 490 500
IGYRTSQYIN RICSTPWSVL VSVIIIIGLI CIASGLRWST TGQLIANTPT
510 520 530 540 550
MIIEEFFLLV LLQAHNWADR QRRVEVTALY ARRRILLSYV EKRFPEVMML

EK
Length:552
Mass (Da):62,792
Last modified:October 1, 1996 - v2
Checksum:i9AB1AEA5DC6BA08E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti283 – 2831V → I in AAA53129 (PubMed:7929320).Curated
Sequence conflicti441 – 4411F → L in AAA53129 (PubMed:7929320).Curated
Sequence conflicti450 – 4501T → I in AAA53129 (PubMed:7929320).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L34837 mRNA. Translation: AAA53129.1.
Z54141 Genomic DNA. Translation: CAA90837.1.
BK006946 Genomic DNA. Translation: DAA10221.1.
PIRiS50313.
RefSeqiNP_014052.1. NM_001182832.1.

Genome annotation databases

EnsemblFungiiYMR319C; YMR319C; YMR319C.
GeneIDi855369.
KEGGisce:YMR319C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L34837 mRNA. Translation: AAA53129.1.
Z54141 Genomic DNA. Translation: CAA90837.1.
BK006946 Genomic DNA. Translation: DAA10221.1.
PIRiS50313.
RefSeqiNP_014052.1. NM_001182832.1.

3D structure databases

ProteinModelPortaliP40988.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35499. 48 interactions.
DIPiDIP-5543N.
IntActiP40988. 1 interaction.
MINTiMINT-480025.

Protein family/group databases

TCDBi9.A.9.1.1. the low affinity fe(2+) transporter (fet) family.

PTM databases

iPTMnetiP40988.

Proteomic databases

MaxQBiP40988.
PeptideAtlasiP40988.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR319C; YMR319C; YMR319C.
GeneIDi855369.
KEGGisce:YMR319C.

Organism-specific databases

EuPathDBiFungiDB:YMR319C.
SGDiS000004938. FET4.

Phylogenomic databases

HOGENOMiHOG000111194.
InParanoidiP40988.
KOiK19792.
OMAiNWWLIIG.
OrthoDBiEOG7S2290.

Enzyme and pathway databases

BioCyciYEAST:G3O-32983-MONOMER.

Miscellaneous databases

NextBioi979151.
PROiP40988.

Family and domain databases

InterProiIPR007251. Iron_permease_Fet4.
[Graphical view]
PfamiPF04120. Iron_permease. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The FET4 gene encodes the low affinity Fe(II) transport protein of Saccharomyces cerevisiae."
    Dix D.R., Bridgham J.T., Broderius M.A., Byersdorfer C.A., Eide D.J.
    J. Biol. Chem. 269:26092-26099(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  6. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-48 AND SER-50, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiFET4_YEAST
AccessioniPrimary (citable) accession number: P40988
Secondary accession number(s): D6W0E7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: October 1, 1996
Last modified: May 11, 2016
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 573 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.