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Protein

Low-affinity Fe(2+) transport protein

Gene

FET4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for Fe2+ ion low affinity uptake.

GO - Molecular functioni

  • copper ion transmembrane transporter activity Source: SGD
  • iron ion transmembrane transporter activity Source: SGD

GO - Biological processi

  • copper ion import Source: SGD
  • intracellular copper ion transport Source: SGD
  • iron ion homeostasis Source: UniProtKB-KW
  • low-affinity iron ion transmembrane transport Source: SGD
  • zinc II ion transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Ion transport, Iron transport, Transport

Keywords - Ligandi

Iron

Enzyme and pathway databases

BioCyciYEAST:G3O-32983-MONOMER.

Protein family/group databases

TCDBi9.A.9.1.1. the low affinity fe(2+) transporter (fet) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Low-affinity Fe(2+) transport protein
Alternative name(s):
Low-affinity Fe(II) transport protein
Gene namesi
Name:FET4
Ordered Locus Names:YMR319C
ORF Names:YM9924.11C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR319C.
SGDiS000004938. FET4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 97ExtracellularSequence analysisAdd BLAST97
Transmembranei98 – 118HelicalSequence analysisAdd BLAST21
Topological domaini119 – 225CytoplasmicSequence analysisAdd BLAST107
Transmembranei226 – 246HelicalSequence analysisAdd BLAST21
Topological domaini247 – 271ExtracellularSequence analysisAdd BLAST25
Transmembranei272 – 292HelicalSequence analysisAdd BLAST21
Topological domaini293 – 354CytoplasmicSequence analysisAdd BLAST62
Transmembranei355 – 375HelicalSequence analysisAdd BLAST21
Topological domaini376 – 383ExtracellularSequence analysis8
Transmembranei384 – 404HelicalSequence analysisAdd BLAST21
Topological domaini405 – 465CytoplasmicSequence analysisAdd BLAST61
Transmembranei466 – 486HelicalSequence analysisAdd BLAST21
Topological domaini487 – 493ExtracellularSequence analysis7
Transmembranei494 – 514HelicalSequence analysisAdd BLAST21
Topological domaini515 – 552CytoplasmicSequence analysisAdd BLAST38

GO - Cellular componenti

  • integral component of plasma membrane Source: SGD
  • intracellular Source: GOC
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000872331 – 552Low-affinity Fe(2+) transport proteinAdd BLAST552

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki39Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei48PhosphoserineCombined sources1
Modified residuei50PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP40988.
PRIDEiP40988.

PTM databases

iPTMnetiP40988.

Expressioni

Inductioni

By iron deprivation.

Interactioni

Protein-protein interaction databases

BioGridi35499. 48 interactors.
DIPiDIP-5543N.
IntActiP40988. 1 interactor.
MINTiMINT-480025.

Structurei

3D structure databases

ProteinModelPortaliP40988.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the FET4 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000111194.
InParanoidiP40988.
KOiK19792.
OMAiWDTLLMR.
OrthoDBiEOG092C1LTM.

Family and domain databases

InterProiIPR007251. Iron_permease_Fet4.
[Graphical view]
PfamiPF04120. Iron_permease. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40988-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKIAEFLGN PGARPDVHHR APTVDCKQYE EFGDSNDYKN DDVVRVVSHS
60 70 80 90 100
DESTDDELCN VNLTETGAIF TSKGFTGLSK GFTDKTLDFL VRVAGSQAVF
110 120 130 140 150
FIVWIILIIW VVIGIVYNAP FNWQVVMQDG QSIQSYVWDT LLMRQQLMST
160 170 180 190 200
HEQILICGRL KSRLASFKNY LTRSTPEEEK ADCTVEANEV SSVENHIDPS
210 220 230 240 250
AINGELPVEN WYDRLSNVAS RYMGSIAAMV IFWIGIFVWI GCGAIPKDAG
260 270 280 290 300
NTPPYTGETT GSNPRLKKFS DAWQMYINTA VAVSLLICTT FLQNIRARHD
310 320 330 340 350
YFTGRFLVDI FDMDEKIDYR IRKHFNDFET PHPVVTIESK KRSTGRKMID
360 370 380 390 400
WYADIIGTGI GVLIGVAVFA TWIGIGSPMK WDDNWWLIIG TYTGLIGFLD
410 420 430 440 450
GFVLREVYFR IVQHEEKNYS DVAKEDLELF QELGIECPEE FSGKAPEINT
460 470 480 490 500
IGYRTSQYIN RICSTPWSVL VSVIIIIGLI CIASGLRWST TGQLIANTPT
510 520 530 540 550
MIIEEFFLLV LLQAHNWADR QRRVEVTALY ARRRILLSYV EKRFPEVMML

EK
Length:552
Mass (Da):62,792
Last modified:October 1, 1996 - v2
Checksum:i9AB1AEA5DC6BA08E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti283V → I in AAA53129 (PubMed:7929320).Curated1
Sequence conflicti441F → L in AAA53129 (PubMed:7929320).Curated1
Sequence conflicti450T → I in AAA53129 (PubMed:7929320).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L34837 mRNA. Translation: AAA53129.1.
Z54141 Genomic DNA. Translation: CAA90837.1.
BK006946 Genomic DNA. Translation: DAA10221.1.
PIRiS50313.
RefSeqiNP_014052.1. NM_001182832.1.

Genome annotation databases

EnsemblFungiiYMR319C; YMR319C; YMR319C.
GeneIDi855369.
KEGGisce:YMR319C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L34837 mRNA. Translation: AAA53129.1.
Z54141 Genomic DNA. Translation: CAA90837.1.
BK006946 Genomic DNA. Translation: DAA10221.1.
PIRiS50313.
RefSeqiNP_014052.1. NM_001182832.1.

3D structure databases

ProteinModelPortaliP40988.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35499. 48 interactors.
DIPiDIP-5543N.
IntActiP40988. 1 interactor.
MINTiMINT-480025.

Protein family/group databases

TCDBi9.A.9.1.1. the low affinity fe(2+) transporter (fet) family.

PTM databases

iPTMnetiP40988.

Proteomic databases

MaxQBiP40988.
PRIDEiP40988.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR319C; YMR319C; YMR319C.
GeneIDi855369.
KEGGisce:YMR319C.

Organism-specific databases

EuPathDBiFungiDB:YMR319C.
SGDiS000004938. FET4.

Phylogenomic databases

HOGENOMiHOG000111194.
InParanoidiP40988.
KOiK19792.
OMAiWDTLLMR.
OrthoDBiEOG092C1LTM.

Enzyme and pathway databases

BioCyciYEAST:G3O-32983-MONOMER.

Miscellaneous databases

PROiP40988.

Family and domain databases

InterProiIPR007251. Iron_permease_Fet4.
[Graphical view]
PfamiPF04120. Iron_permease. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFET4_YEAST
AccessioniPrimary (citable) accession number: P40988
Secondary accession number(s): D6W0E7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 573 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.