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Protein

Low-affinity Fe(2+) transport protein

Gene

FET4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for Fe2+ ion low affinity uptake.

Miscellaneous

Present with 573 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • copper ion transmembrane transporter activity Source: SGD
  • iron ion transmembrane transporter activity Source: SGD

GO - Biological processi

  • copper ion import Source: SGD
  • intracellular copper ion transport Source: SGD
  • iron ion homeostasis Source: UniProtKB-KW
  • low-affinity iron ion transmembrane transport Source: SGD
  • zinc II ion transport Source: SGD

Keywordsi

Biological processIon transport, Iron transport, Transport
LigandIron

Enzyme and pathway databases

BioCyciYEAST:G3O-32983-MONOMER.

Protein family/group databases

TCDBi9.A.9.1.1. the low affinity fe(2+) transporter (fet) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Low-affinity Fe(2+) transport protein
Alternative name(s):
Low-affinity Fe(II) transport protein
Gene namesi
Name:FET4
Ordered Locus Names:YMR319C
ORF Names:YM9924.11C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR319C.
SGDiS000004938. FET4.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 97ExtracellularSequence analysisAdd BLAST97
Transmembranei98 – 118HelicalSequence analysisAdd BLAST21
Topological domaini119 – 225CytoplasmicSequence analysisAdd BLAST107
Transmembranei226 – 246HelicalSequence analysisAdd BLAST21
Topological domaini247 – 271ExtracellularSequence analysisAdd BLAST25
Transmembranei272 – 292HelicalSequence analysisAdd BLAST21
Topological domaini293 – 354CytoplasmicSequence analysisAdd BLAST62
Transmembranei355 – 375HelicalSequence analysisAdd BLAST21
Topological domaini376 – 383ExtracellularSequence analysis8
Transmembranei384 – 404HelicalSequence analysisAdd BLAST21
Topological domaini405 – 465CytoplasmicSequence analysisAdd BLAST61
Transmembranei466 – 486HelicalSequence analysisAdd BLAST21
Topological domaini487 – 493ExtracellularSequence analysis7
Transmembranei494 – 514HelicalSequence analysisAdd BLAST21
Topological domaini515 – 552CytoplasmicSequence analysisAdd BLAST38

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000872331 – 552Low-affinity Fe(2+) transport proteinAdd BLAST552

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki39Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei48PhosphoserineCombined sources1
Modified residuei50PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP40988.
PRIDEiP40988.

PTM databases

iPTMnetiP40988.

Expressioni

Inductioni

By iron deprivation.

Interactioni

Protein-protein interaction databases

BioGridi35499. 95 interactors.
DIPiDIP-5543N.
IntActiP40988. 16 interactors.
MINTiMINT-480025.
STRINGi4932.YMR319C.

Structurei

3D structure databases

ProteinModelPortaliP40988.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the FET4 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000111194.
InParanoidiP40988.
KOiK19792.
OMAiWDTLLMR.
OrthoDBiEOG092C1LTM.

Family and domain databases

InterProiView protein in InterPro
IPR007251. Iron_permease_Fet4.
PfamiView protein in Pfam
PF04120. Iron_permease. 2 hits.

Sequencei

Sequence statusi: Complete.

P40988-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKIAEFLGN PGARPDVHHR APTVDCKQYE EFGDSNDYKN DDVVRVVSHS
60 70 80 90 100
DESTDDELCN VNLTETGAIF TSKGFTGLSK GFTDKTLDFL VRVAGSQAVF
110 120 130 140 150
FIVWIILIIW VVIGIVYNAP FNWQVVMQDG QSIQSYVWDT LLMRQQLMST
160 170 180 190 200
HEQILICGRL KSRLASFKNY LTRSTPEEEK ADCTVEANEV SSVENHIDPS
210 220 230 240 250
AINGELPVEN WYDRLSNVAS RYMGSIAAMV IFWIGIFVWI GCGAIPKDAG
260 270 280 290 300
NTPPYTGETT GSNPRLKKFS DAWQMYINTA VAVSLLICTT FLQNIRARHD
310 320 330 340 350
YFTGRFLVDI FDMDEKIDYR IRKHFNDFET PHPVVTIESK KRSTGRKMID
360 370 380 390 400
WYADIIGTGI GVLIGVAVFA TWIGIGSPMK WDDNWWLIIG TYTGLIGFLD
410 420 430 440 450
GFVLREVYFR IVQHEEKNYS DVAKEDLELF QELGIECPEE FSGKAPEINT
460 470 480 490 500
IGYRTSQYIN RICSTPWSVL VSVIIIIGLI CIASGLRWST TGQLIANTPT
510 520 530 540 550
MIIEEFFLLV LLQAHNWADR QRRVEVTALY ARRRILLSYV EKRFPEVMML

EK
Length:552
Mass (Da):62,792
Last modified:October 1, 1996 - v2
Checksum:i9AB1AEA5DC6BA08E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti283V → I in AAA53129 (PubMed:7929320).Curated1
Sequence conflicti441F → L in AAA53129 (PubMed:7929320).Curated1
Sequence conflicti450T → I in AAA53129 (PubMed:7929320).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L34837 mRNA. Translation: AAA53129.1.
Z54141 Genomic DNA. Translation: CAA90837.1.
BK006946 Genomic DNA. Translation: DAA10221.1.
PIRiS50313.
RefSeqiNP_014052.1. NM_001182832.1.

Genome annotation databases

EnsemblFungiiYMR319C; YMR319C; YMR319C.
GeneIDi855369.
KEGGisce:YMR319C.

Similar proteinsi

Entry informationi

Entry nameiFET4_YEAST
AccessioniPrimary (citable) accession number: P40988
Secondary accession number(s): D6W0E7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: October 1, 1996
Last modified: November 22, 2017
This is version 137 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names