P40985 (HUL4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable E3 ubiquitin-protein ligase HUL4
EC=6.3.2.-

Alternative name(s):
HECT ubiquitin ligase 4

Gene names

Name:	HUL4
Ordered Locus Names:	YJR036C
ORF Names:	J1608

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	892 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Probable E3 ubiquitin-protein ligase, component of the TRAMP (TRF4) complex which has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism limiting inappropriate expression of genetic information. Polyadenylation is required for the degradative activity of the exosome on several of its nuclear RNA substrates. Ref.6
Subunit structure	Component of the TRAMP complex (also called TRF4 complex) composed of at least HUL4, MTR4, PAP2/TRF4 and either AIR1 or AIR2. Ref.6
Subcellular location	Nucleus Probable.
Sequence similarities	Belongs to the HUL4 family. Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Nucleus
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein ubiquitination involved in ubiquitin-dependent protein catabolic process Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ubiquitin-protein ligase activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 892	892	Probable E3 ubiquitin-protein ligase HUL4		PRO_0000084094
Regions
	Domain	792 – 892	101	HECT
Sites
	Active site	860	1	Glycyl thioester intermediate By similarity
Experimental info
	Sequence conflict	362	1	H → Q in CAA57291. Ref.4
	Sequence conflict	410	1	T → M in CAA57291. Ref.4
	Sequence conflict	418	1	T → I in CAA57291. Ref.4
	Sequence conflict	514 – 519	6	GKSVDV → RQIRRR in CAA57291. Ref.4
	Sequence conflict	589 – 607	19	ESSRS…DKSKG → KKAHVHGLPLTLQILTNQR in CAA57291. Ref.4
	Sequence conflict	705	1	V → A in CAA57291. Ref.4
	Sequence conflict	723 – 724	2	KH → ND in CAA57291. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P40985 [UniParc]. Last modified November 1, 1995. Version 2. Checksum: 35EF9A8DDA92BD84	FASTA	892	103,457
	10 20 30 40 50 60 MVSLFDKLNA KKDGRDGSVS KELLSHSVAH TKNRLPKSGR RTSERSLAAS VKDGSCSNSK 70 80 90 100 110 120 SNKRNSSASV SGEEDKSCLI SLNCLCCGVP LRFPASITKF RCSACQVTVI VKEPEINSNL 130 140 150 160 170 180 ESSTHISCTL EGLQMVVRRC HDDLQRLKKT GILDKERKGL IFQPVITYLL DRFHDVSILN 190 200 210 220 230 240 RSFLVHDGGK NIKMLNYEVL QRFYSILSNL PTRKPYYSML CCCNDLLKRI TINKGENLQI 250 260 270 280 290 300 LQYRWLLIIL NIPTIRTCLI RDRKSKNVFE TQQIRAVSYE LAKRCIGYLS NLSTKTSQQL 310 320 330 340 350 360 IQSLRRTPTD NFSYQVEILN LYINFQFSRL LSNELSNRTA KNNVKPEDEM RSRLRRHHTT 370 380 390 400 410 420 GHEFLSTRPI SAQSNDKQGS GFTHPVNNKM KFKFFQYEED WHIHSAAKLT FIYYVANTRR 430 440 450 460 470 480 NGRGALSIQS FYNITLDFID YKQDFDHWRG VAQKTKMNQL IEEWGNSTTK KCFSFCKYPF 490 500 510 520 530 540 ILSLGIKISI MEYEIRRIME HEAEQAFLIS LDKGKSVDVY FKIKVRRDVI SHDSLRCIKE 550 560 570 580 590 600 HQGDLLKSLR IEFVNEPGID AGGLRKEWFF LLTKSLFNPM NGLFIYIKES SRSWFAIDPP 610 620 630 640 650 660 NFDKSKGKNS QLELYYLFGV VMGLAIFNST ILDLQFPKAL YKKLCSEPLS FEDYSELFPE 670 680 690 700 710 720 TSRNLIKMLN YTEDNFEDVF SLTFETTYRN NNWILNDSKS SKEYVTVELC ENGRNVPITQ 730 740 750 760 770 780 SNKHEFVMKW VEFYLEKSIE PQYNKFVSGF KRVFAECNSI KLFNSEELER LVCGDEEQTK 790 800 810 820 830 840 FDFKSLRSVT KYVGGFSDDS RAVCWFWEII ESWDYPLQKK LLQFVTASDR IPATGISTIP 850 860 870 880 890 FKISLLGSHD SDDLPLAHTC FNEICLWNYS SKKKLELKLL WAINESEGYG FR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of a 42.5 kb DNA sequence of chromosome X reveals three tRNA genes and 14 new open reading frames including a gene most probably belonging to the family of ubiquitin-protein ligases." Huang M.-E., Chuat J.-C., Galibert F. Yeast 11:775-781(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"RAD26, the functional S. cerevisiae homolog of the Cockayne syndrome B gene ERCC6." van Gool A.J., Verhage R., Swagemakers S.M.A., van de Putte P., Brouwer J., Troelstra C., Bootsma D., Hoeijmakers J.H.J. EMBO J. 13:5361-5369(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 362-892.
[5]	"Functional domains of the rsp5 ubiquitin-protein ligase." Wang G., Yang J., Huibregtse J.M. Mol. Cell. Biol. 19:342-352(1999) [PubMed] [Europe PMC] [Abstract] Cited for: GENE NAME, GENE DISRUPTION.
[6]	"A new yeast poly(A) polymerase complex involved in RNA quality control." Vanacova S., Wolf J., Martin G., Blank D., Dettwiler S., Friedlein A., Langen H., Keith G., Keller W. PLoS Biol. 3:986-997(2005) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE TRF4 COMPLEX, MASS SPECTROMETRY, FUNCTION OF THE TRF4 COMPLEX.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L36344 Genomic DNA. Translation: AAA88738.1. Z49536 Genomic DNA. Translation: CAA89563.1. X81635 Genomic DNA. Translation: CAA57291.1. BK006943 Genomic DNA. Translation: DAA08825.1.
PIR	S57055.
RefSeq	NP_012570.3. NM_001181694.3.
3D structure databases
ProteinModelPortal	P40985.
SMR	P40985. Positions 520-883.
ModBase	Search...
Protein-protein interaction databases
IntAct	P40985. 1 interaction.
MINT	MINT-2782607.
STRING	4932.YJR036C.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJR036C; YJR036C; YJR036C.
GeneID	853494.
KEGG	sce:YJR036C. sce:YJR041C.
Organism-specific databases
CYGD	YJR036c.
SGD	S000003797. HUL4.
Phylogenomic databases
eggNOG	COG5021.
GeneTree	ENSGT00660000095216.
HOGENOM	HOG000246763.
KO	K12232. K14862.
OMA	NEPGIDA.
OrthoDB	EOG42Z7Z7.
Gene expression databases
Genevestigator	P40985.
GermOnline	YJR036C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000569. HECT. [Graphical view]
Pfam	PF00632. HECT. 1 hit. [Graphical view]
SMART	SM00119. HECTc. 1 hit. [Graphical view]
SUPFAM	SSF56204. HECT. 1 hit.
PROSITE	PS50237. HECT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974128.

Entry information

Entry name

HUL4_YEAST

Accession

Primary (citable) accession number: P40985
Secondary accession number(s): D6VWK9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 101 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program