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Protein

Probable E3 ubiquitin-protein ligase HUL4

Gene

HUL4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Probable E3 ubiquitin-protein ligase, component of the TRAMP (TRF4) complex which has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism limiting inappropriate expression of genetic information. Polyadenylation is required for the degradative activity of the exosome on several of its nuclear RNA substrates.1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei860Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • ubiquitin protein ligase activity Source: GO_Central
  • ubiquitin-protein transferase activity Source: SGD

GO - Biological processi

  • protein ubiquitination Source: SGD

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-31673-MONOMER.
ReactomeiR-SCE-1169408. ISG15 antiviral mechanism.
R-SCE-3108214. SUMOylation of DNA damage response and repair proteins.
R-SCE-5693565. Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks.
R-SCE-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable E3 ubiquitin-protein ligase HUL4 (EC:2.3.2.26)
Alternative name(s):
HECT ubiquitin ligase 4
HECT-type E3 ubiquitin transferase HUL4
Gene namesi
Name:HUL4
Ordered Locus Names:YJR036C
ORF Names:J1608
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR036C.
SGDiS000003797. HUL4.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000840941 – 892Probable E3 ubiquitin-protein ligase HUL4Add BLAST892

Proteomic databases

PRIDEiP40985.

Interactioni

Subunit structurei

Component of the TRAMP complex (also called TRF4 complex) composed of at least HUL4, MTR4, PAP2/TRF4 and either AIR1 or AIR2.1 Publication

Protein-protein interaction databases

BioGridi33789. 51 interactors.
IntActiP40985. 1 interactor.
MINTiMINT-2782607.
STRINGi4932.YJR036C.

Structurei

3D structure databases

ProteinModelPortaliP40985.
SMRiP40985.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini792 – 892HECTPROSITE-ProRule annotationAdd BLAST101

Sequence similaritiesi

Belongs to the HUL4 family.Curated

Phylogenomic databases

GeneTreeiENSGT00900000140848.
HOGENOMiHOG000246763.
InParanoidiP40985.
KOiK12232.
OMAiKRCIGYL.
OrthoDBiEOG092C12Z2.

Family and domain databases

CDDicd00078. HECTc. 1 hit.
InterProiView protein in InterPro
IPR000569. HECT_dom.
IPR035983. Hect_E3_ubiquitin_ligase.
PfamiView protein in Pfam
PF00632. HECT. 1 hit.
SMARTiView protein in SMART
SM00119. HECTc. 1 hit.
SUPFAMiSSF56204. SSF56204. 1 hit.
PROSITEiView protein in PROSITE
PS50237. HECT. 1 hit.

Sequencei

Sequence statusi: Complete.

P40985-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSLFDKLNA KKDGRDGSVS KELLSHSVAH TKNRLPKSGR RTSERSLAAS
60 70 80 90 100
VKDGSCSNSK SNKRNSSASV SGEEDKSCLI SLNCLCCGVP LRFPASITKF
110 120 130 140 150
RCSACQVTVI VKEPEINSNL ESSTHISCTL EGLQMVVRRC HDDLQRLKKT
160 170 180 190 200
GILDKERKGL IFQPVITYLL DRFHDVSILN RSFLVHDGGK NIKMLNYEVL
210 220 230 240 250
QRFYSILSNL PTRKPYYSML CCCNDLLKRI TINKGENLQI LQYRWLLIIL
260 270 280 290 300
NIPTIRTCLI RDRKSKNVFE TQQIRAVSYE LAKRCIGYLS NLSTKTSQQL
310 320 330 340 350
IQSLRRTPTD NFSYQVEILN LYINFQFSRL LSNELSNRTA KNNVKPEDEM
360 370 380 390 400
RSRLRRHHTT GHEFLSTRPI SAQSNDKQGS GFTHPVNNKM KFKFFQYEED
410 420 430 440 450
WHIHSAAKLT FIYYVANTRR NGRGALSIQS FYNITLDFID YKQDFDHWRG
460 470 480 490 500
VAQKTKMNQL IEEWGNSTTK KCFSFCKYPF ILSLGIKISI MEYEIRRIME
510 520 530 540 550
HEAEQAFLIS LDKGKSVDVY FKIKVRRDVI SHDSLRCIKE HQGDLLKSLR
560 570 580 590 600
IEFVNEPGID AGGLRKEWFF LLTKSLFNPM NGLFIYIKES SRSWFAIDPP
610 620 630 640 650
NFDKSKGKNS QLELYYLFGV VMGLAIFNST ILDLQFPKAL YKKLCSEPLS
660 670 680 690 700
FEDYSELFPE TSRNLIKMLN YTEDNFEDVF SLTFETTYRN NNWILNDSKS
710 720 730 740 750
SKEYVTVELC ENGRNVPITQ SNKHEFVMKW VEFYLEKSIE PQYNKFVSGF
760 770 780 790 800
KRVFAECNSI KLFNSEELER LVCGDEEQTK FDFKSLRSVT KYVGGFSDDS
810 820 830 840 850
RAVCWFWEII ESWDYPLQKK LLQFVTASDR IPATGISTIP FKISLLGSHD
860 870 880 890
SDDLPLAHTC FNEICLWNYS SKKKLELKLL WAINESEGYG FR
Length:892
Mass (Da):103,457
Last modified:November 1, 1995 - v2
Checksum:i35EF9A8DDA92BD84
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti362H → Q in CAA57291 (PubMed:7957102).Curated1
Sequence conflicti410T → M in CAA57291 (PubMed:7957102).Curated1
Sequence conflicti418T → I in CAA57291 (PubMed:7957102).Curated1
Sequence conflicti514 – 519GKSVDV → RQIRRR in CAA57291 (PubMed:7957102).Curated6
Sequence conflicti589 – 607ESSRS…DKSKG → KKAHVHGLPLTLQILTNQR in CAA57291 (PubMed:7957102).CuratedAdd BLAST19
Sequence conflicti705V → A in CAA57291 (PubMed:7957102).Curated1
Sequence conflicti723 – 724KH → ND in CAA57291 (PubMed:7957102).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36344 Genomic DNA. Translation: AAA88738.1.
Z49536 Genomic DNA. Translation: CAA89563.1.
X81635 Genomic DNA. Translation: CAA57291.1.
BK006943 Genomic DNA. Translation: DAA08825.1.
PIRiS57055.
RefSeqiNP_012570.3. NM_001181694.3.

Genome annotation databases

EnsemblFungiiYJR036C; YJR036C; YJR036C.
GeneIDi853494.
KEGGisce:YJR036C.

Similar proteinsi

Entry informationi

Entry nameiHUL4_YEAST
AccessioniPrimary (citable) accession number: P40985
Secondary accession number(s): D6VWK9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 1, 1995
Last modified: November 22, 2017
This is version 141 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names