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P40985 (HUL4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable E3 ubiquitin-protein ligase HUL4

EC=6.3.2.-
Alternative name(s):
HECT ubiquitin ligase 4
Gene names
Name:HUL4
Ordered Locus Names:YJR036C
ORF Names:J1608
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length892 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probable E3 ubiquitin-protein ligase, component of the TRAMP (TRF4) complex which has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism limiting inappropriate expression of genetic information. Polyadenylation is required for the degradative activity of the exosome on several of its nuclear RNA substrates. Ref.6

Subunit structure

Component of the TRAMP complex (also called TRF4 complex) composed of at least HUL4, MTR4, PAP2/TRF4 and either AIR1 or AIR2. Ref.6

Subcellular location

Nucleus Probable.

Sequence similarities

Belongs to the HUL4 family.

Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   Cellular componentNucleus
   Molecular functionLigase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processprotein ubiquitination

Inferred from sequence or structural similarity Ref.5. Source: SGD

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionubiquitin-protein ligase activity

Inferred from sequence or structural similarity Ref.5. Source: SGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 892892Probable E3 ubiquitin-protein ligase HUL4
PRO_0000084094

Regions

Domain792 – 892101HECT

Sites

Active site8601Glycyl thioester intermediate By similarity

Experimental info

Sequence conflict3621H → Q in CAA57291. Ref.4
Sequence conflict4101T → M in CAA57291. Ref.4
Sequence conflict4181T → I in CAA57291. Ref.4
Sequence conflict514 – 5196GKSVDV → RQIRRR in CAA57291. Ref.4
Sequence conflict589 – 60719ESSRS…DKSKG → KKAHVHGLPLTLQILTNQR in CAA57291. Ref.4
Sequence conflict7051V → A in CAA57291. Ref.4
Sequence conflict723 – 7242KH → ND in CAA57291. Ref.4

Sequences

Sequence LengthMass (Da)Tools
P40985 [UniParc].

Last modified November 1, 1995. Version 2.
Checksum: 35EF9A8DDA92BD84

FASTA892103,457
        10         20         30         40         50         60 
MVSLFDKLNA KKDGRDGSVS KELLSHSVAH TKNRLPKSGR RTSERSLAAS VKDGSCSNSK 

        70         80         90        100        110        120 
SNKRNSSASV SGEEDKSCLI SLNCLCCGVP LRFPASITKF RCSACQVTVI VKEPEINSNL 

       130        140        150        160        170        180 
ESSTHISCTL EGLQMVVRRC HDDLQRLKKT GILDKERKGL IFQPVITYLL DRFHDVSILN 

       190        200        210        220        230        240 
RSFLVHDGGK NIKMLNYEVL QRFYSILSNL PTRKPYYSML CCCNDLLKRI TINKGENLQI 

       250        260        270        280        290        300 
LQYRWLLIIL NIPTIRTCLI RDRKSKNVFE TQQIRAVSYE LAKRCIGYLS NLSTKTSQQL 

       310        320        330        340        350        360 
IQSLRRTPTD NFSYQVEILN LYINFQFSRL LSNELSNRTA KNNVKPEDEM RSRLRRHHTT 

       370        380        390        400        410        420 
GHEFLSTRPI SAQSNDKQGS GFTHPVNNKM KFKFFQYEED WHIHSAAKLT FIYYVANTRR 

       430        440        450        460        470        480 
NGRGALSIQS FYNITLDFID YKQDFDHWRG VAQKTKMNQL IEEWGNSTTK KCFSFCKYPF 

       490        500        510        520        530        540 
ILSLGIKISI MEYEIRRIME HEAEQAFLIS LDKGKSVDVY FKIKVRRDVI SHDSLRCIKE 

       550        560        570        580        590        600 
HQGDLLKSLR IEFVNEPGID AGGLRKEWFF LLTKSLFNPM NGLFIYIKES SRSWFAIDPP 

       610        620        630        640        650        660 
NFDKSKGKNS QLELYYLFGV VMGLAIFNST ILDLQFPKAL YKKLCSEPLS FEDYSELFPE 

       670        680        690        700        710        720 
TSRNLIKMLN YTEDNFEDVF SLTFETTYRN NNWILNDSKS SKEYVTVELC ENGRNVPITQ 

       730        740        750        760        770        780 
SNKHEFVMKW VEFYLEKSIE PQYNKFVSGF KRVFAECNSI KLFNSEELER LVCGDEEQTK 

       790        800        810        820        830        840 
FDFKSLRSVT KYVGGFSDDS RAVCWFWEII ESWDYPLQKK LLQFVTASDR IPATGISTIP 

       850        860        870        880        890 
FKISLLGSHD SDDLPLAHTC FNEICLWNYS SKKKLELKLL WAINESEGYG FR 

« Hide

References

« Hide 'large scale' references
[1]"Analysis of a 42.5 kb DNA sequence of chromosome X reveals three tRNA genes and 14 new open reading frames including a gene most probably belonging to the family of ubiquitin-protein ligases."
Huang M.-E., Chuat J.-C., Galibert F.
Yeast 11:775-781(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"RAD26, the functional S. cerevisiae homolog of the Cockayne syndrome B gene ERCC6."
van Gool A.J., Verhage R., Swagemakers S.M.A., van de Putte P., Brouwer J., Troelstra C., Bootsma D., Hoeijmakers J.H.J.
EMBO J. 13:5361-5369(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 362-892.
[5]"Functional domains of the rsp5 ubiquitin-protein ligase."
Wang G., Yang J., Huibregtse J.M.
Mol. Cell. Biol. 19:342-352(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE NAME, GENE DISRUPTION.
[6]"A new yeast poly(A) polymerase complex involved in RNA quality control."
Vanacova S., Wolf J., Martin G., Blank D., Dettwiler S., Friedlein A., Langen H., Keith G., Keller W.
PLoS Biol. 3:986-997(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE TRF4 COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION OF THE TRF4 COMPLEX.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L36344 Genomic DNA. Translation: AAA88738.1.
Z49536 Genomic DNA. Translation: CAA89563.1.
X81635 Genomic DNA. Translation: CAA57291.1.
BK006943 Genomic DNA. Translation: DAA08825.1.
PIRS57055.
RefSeqNP_012570.3. NM_001181694.3.

3D structure databases

ProteinModelPortalP40985.
SMRP40985. Positions 477-885.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33789. 27 interactions.
IntActP40985. 1 interaction.
MINTMINT-2782607.
STRING4932.YJR036C.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJR036C; YJR036C; YJR036C.
GeneID853494.
KEGGsce:YJR036C.

Organism-specific databases

CYGDYJR036c.
SGDS000003797. HUL4.

Phylogenomic databases

eggNOGCOG5021.
GeneTreeENSGT00750000117423.
HOGENOMHOG000246763.
KOK12232.
OMAQYGNDWH.
OrthoDBEOG70GMQ0.

Enzyme and pathway databases

BioCycYEAST:G3O-31673-MONOMER.

Gene expression databases

GenevestigatorP40985.

Family and domain databases

InterProIPR000569. HECT.
[Graphical view]
PfamPF00632. HECT. 1 hit.
[Graphical view]
SMARTSM00119. HECTc. 1 hit.
[Graphical view]
SUPFAMSSF56204. SSF56204. 1 hit.
PROSITEPS50237. HECT. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio974128.

Entry information

Entry nameHUL4_YEAST
AccessionPrimary (citable) accession number: P40985
Secondary accession number(s): D6VWK9
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 1, 1995
Last modified: April 16, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families