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Protein

Probable E3 ubiquitin-protein ligase HUL4

Gene

HUL4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Probable E3 ubiquitin-protein ligase, component of the TRAMP (TRF4) complex which has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism limiting inappropriate expression of genetic information. Polyadenylation is required for the degradative activity of the exosome on several of its nuclear RNA substrates.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei860 – 8601Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. ligase activity Source: UniProtKB-KW
  2. ubiquitin-protein transferase activity Source: SGD

GO - Biological processi

  1. protein ubiquitination Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-31673-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable E3 ubiquitin-protein ligase HUL4 (EC:6.3.2.-)
Alternative name(s):
HECT ubiquitin ligase 4
Gene namesi
Name:HUL4
Ordered Locus Names:YJR036C
ORF Names:J1608
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome X

Organism-specific databases

CYGDiYJR036c.
SGDiS000003797. HUL4.

Subcellular locationi

Nucleus Curated

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 892892Probable E3 ubiquitin-protein ligase HUL4PRO_0000084094Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriP40985.

Interactioni

Subunit structurei

Component of the TRAMP complex (also called TRF4 complex) composed of at least HUL4, MTR4, PAP2/TRF4 and either AIR1 or AIR2.1 Publication

Protein-protein interaction databases

BioGridi33789. 27 interactions.
IntActiP40985. 1 interaction.
MINTiMINT-2782607.
STRINGi4932.YJR036C.

Structurei

3D structure databases

ProteinModelPortaliP40985.
SMRiP40985. Positions 520-883.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini792 – 892101HECTPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the HUL4 family.Curated
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5021.
GeneTreeiENSGT00780000121841.
HOGENOMiHOG000246763.
InParanoidiP40985.
KOiK12232.
OMAiQYGNDWH.
OrthoDBiEOG70GMQ0.

Family and domain databases

InterProiIPR000569. HECT.
[Graphical view]
PfamiPF00632. HECT. 1 hit.
[Graphical view]
SMARTiSM00119. HECTc. 1 hit.
[Graphical view]
SUPFAMiSSF56204. SSF56204. 1 hit.
PROSITEiPS50237. HECT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40985-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVSLFDKLNA KKDGRDGSVS KELLSHSVAH TKNRLPKSGR RTSERSLAAS
60 70 80 90 100
VKDGSCSNSK SNKRNSSASV SGEEDKSCLI SLNCLCCGVP LRFPASITKF
110 120 130 140 150
RCSACQVTVI VKEPEINSNL ESSTHISCTL EGLQMVVRRC HDDLQRLKKT
160 170 180 190 200
GILDKERKGL IFQPVITYLL DRFHDVSILN RSFLVHDGGK NIKMLNYEVL
210 220 230 240 250
QRFYSILSNL PTRKPYYSML CCCNDLLKRI TINKGENLQI LQYRWLLIIL
260 270 280 290 300
NIPTIRTCLI RDRKSKNVFE TQQIRAVSYE LAKRCIGYLS NLSTKTSQQL
310 320 330 340 350
IQSLRRTPTD NFSYQVEILN LYINFQFSRL LSNELSNRTA KNNVKPEDEM
360 370 380 390 400
RSRLRRHHTT GHEFLSTRPI SAQSNDKQGS GFTHPVNNKM KFKFFQYEED
410 420 430 440 450
WHIHSAAKLT FIYYVANTRR NGRGALSIQS FYNITLDFID YKQDFDHWRG
460 470 480 490 500
VAQKTKMNQL IEEWGNSTTK KCFSFCKYPF ILSLGIKISI MEYEIRRIME
510 520 530 540 550
HEAEQAFLIS LDKGKSVDVY FKIKVRRDVI SHDSLRCIKE HQGDLLKSLR
560 570 580 590 600
IEFVNEPGID AGGLRKEWFF LLTKSLFNPM NGLFIYIKES SRSWFAIDPP
610 620 630 640 650
NFDKSKGKNS QLELYYLFGV VMGLAIFNST ILDLQFPKAL YKKLCSEPLS
660 670 680 690 700
FEDYSELFPE TSRNLIKMLN YTEDNFEDVF SLTFETTYRN NNWILNDSKS
710 720 730 740 750
SKEYVTVELC ENGRNVPITQ SNKHEFVMKW VEFYLEKSIE PQYNKFVSGF
760 770 780 790 800
KRVFAECNSI KLFNSEELER LVCGDEEQTK FDFKSLRSVT KYVGGFSDDS
810 820 830 840 850
RAVCWFWEII ESWDYPLQKK LLQFVTASDR IPATGISTIP FKISLLGSHD
860 870 880 890
SDDLPLAHTC FNEICLWNYS SKKKLELKLL WAINESEGYG FR
Length:892
Mass (Da):103,457
Last modified:November 1, 1995 - v2
Checksum:i35EF9A8DDA92BD84
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti362 – 3621H → Q in CAA57291 (PubMed:7957102).Curated
Sequence conflicti410 – 4101T → M in CAA57291 (PubMed:7957102).Curated
Sequence conflicti418 – 4181T → I in CAA57291 (PubMed:7957102).Curated
Sequence conflicti514 – 5196GKSVDV → RQIRRR in CAA57291 (PubMed:7957102).Curated
Sequence conflicti589 – 60719ESSRS…DKSKG → KKAHVHGLPLTLQILTNQR in CAA57291 (PubMed:7957102).CuratedAdd
BLAST
Sequence conflicti705 – 7051V → A in CAA57291 (PubMed:7957102).Curated
Sequence conflicti723 – 7242KH → ND in CAA57291 (PubMed:7957102).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36344 Genomic DNA. Translation: AAA88738.1.
Z49536 Genomic DNA. Translation: CAA89563.1.
X81635 Genomic DNA. Translation: CAA57291.1.
BK006943 Genomic DNA. Translation: DAA08825.1.
PIRiS57055.
RefSeqiNP_012570.3. NM_001181694.3.

Genome annotation databases

EnsemblFungiiYJR036C; YJR036C; YJR036C.
GeneIDi853494.
KEGGisce:YJR036C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36344 Genomic DNA. Translation: AAA88738.1.
Z49536 Genomic DNA. Translation: CAA89563.1.
X81635 Genomic DNA. Translation: CAA57291.1.
BK006943 Genomic DNA. Translation: DAA08825.1.
PIRiS57055.
RefSeqiNP_012570.3. NM_001181694.3.

3D structure databases

ProteinModelPortaliP40985.
SMRiP40985. Positions 520-883.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33789. 27 interactions.
IntActiP40985. 1 interaction.
MINTiMINT-2782607.
STRINGi4932.YJR036C.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJR036C; YJR036C; YJR036C.
GeneIDi853494.
KEGGisce:YJR036C.

Organism-specific databases

CYGDiYJR036c.
SGDiS000003797. HUL4.

Phylogenomic databases

eggNOGiCOG5021.
GeneTreeiENSGT00780000121841.
HOGENOMiHOG000246763.
InParanoidiP40985.
KOiK12232.
OMAiQYGNDWH.
OrthoDBiEOG70GMQ0.

Enzyme and pathway databases

BioCyciYEAST:G3O-31673-MONOMER.

Miscellaneous databases

NextBioi974128.

Gene expression databases

GenevestigatoriP40985.

Family and domain databases

InterProiIPR000569. HECT.
[Graphical view]
PfamiPF00632. HECT. 1 hit.
[Graphical view]
SMARTiSM00119. HECTc. 1 hit.
[Graphical view]
SUPFAMiSSF56204. SSF56204. 1 hit.
PROSITEiPS50237. HECT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of a 42.5 kb DNA sequence of chromosome X reveals three tRNA genes and 14 new open reading frames including a gene most probably belonging to the family of ubiquitin-protein ligases."
    Huang M.-E., Chuat J.-C., Galibert F.
    Yeast 11:775-781(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "RAD26, the functional S. cerevisiae homolog of the Cockayne syndrome B gene ERCC6."
    van Gool A.J., Verhage R., Swagemakers S.M.A., van de Putte P., Brouwer J., Troelstra C., Bootsma D., Hoeijmakers J.H.J.
    EMBO J. 13:5361-5369(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 362-892.
  5. "Functional domains of the rsp5 ubiquitin-protein ligase."
    Wang G., Yang J., Huibregtse J.M.
    Mol. Cell. Biol. 19:342-352(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE NAME, GENE DISRUPTION.
  6. "A new yeast poly(A) polymerase complex involved in RNA quality control."
    Vanacova S., Wolf J., Martin G., Blank D., Dettwiler S., Friedlein A., Langen H., Keith G., Keller W.
    PLoS Biol. 3:986-997(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE TRF4 COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION OF THE TRF4 COMPLEX.

Entry informationi

Entry nameiHUL4_YEAST
AccessioniPrimary (citable) accession number: P40985
Secondary accession number(s): D6VWK9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 1, 1995
Last modified: February 4, 2015
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.