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P40985

- HUL4_YEAST

UniProt

P40985 - HUL4_YEAST

Protein

Probable E3 ubiquitin-protein ligase HUL4

Gene

HUL4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 2 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Probable E3 ubiquitin-protein ligase, component of the TRAMP (TRF4) complex which has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism limiting inappropriate expression of genetic information. Polyadenylation is required for the degradative activity of the exosome on several of its nuclear RNA substrates.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei860 – 8601Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. ligase activity Source: UniProtKB-KW
    2. ubiquitin-protein transferase activity Source: SGD

    GO - Biological processi

    1. protein ubiquitination Source: SGD

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31673-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable E3 ubiquitin-protein ligase HUL4 (EC:6.3.2.-)
    Alternative name(s):
    HECT ubiquitin ligase 4
    Gene namesi
    Name:HUL4
    Ordered Locus Names:YJR036C
    ORF Names:J1608
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJR036c.
    SGDiS000003797. HUL4.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 892892Probable E3 ubiquitin-protein ligase HUL4PRO_0000084094Add
    BLAST

    Expressioni

    Gene expression databases

    GenevestigatoriP40985.

    Interactioni

    Subunit structurei

    Component of the TRAMP complex (also called TRF4 complex) composed of at least HUL4, MTR4, PAP2/TRF4 and either AIR1 or AIR2.1 Publication

    Protein-protein interaction databases

    BioGridi33789. 27 interactions.
    IntActiP40985. 1 interaction.
    MINTiMINT-2782607.
    STRINGi4932.YJR036C.

    Structurei

    3D structure databases

    ProteinModelPortaliP40985.
    SMRiP40985. Positions 520-883.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini792 – 892101HECTPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the HUL4 family.Curated
    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5021.
    GeneTreeiENSGT00750000117423.
    HOGENOMiHOG000246763.
    KOiK12232.
    OMAiQYGNDWH.
    OrthoDBiEOG70GMQ0.

    Family and domain databases

    InterProiIPR000569. HECT.
    [Graphical view]
    PfamiPF00632. HECT. 1 hit.
    [Graphical view]
    SMARTiSM00119. HECTc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56204. SSF56204. 1 hit.
    PROSITEiPS50237. HECT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P40985-1 [UniParc]FASTAAdd to Basket

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    MVSLFDKLNA KKDGRDGSVS KELLSHSVAH TKNRLPKSGR RTSERSLAAS    50
    VKDGSCSNSK SNKRNSSASV SGEEDKSCLI SLNCLCCGVP LRFPASITKF 100
    RCSACQVTVI VKEPEINSNL ESSTHISCTL EGLQMVVRRC HDDLQRLKKT 150
    GILDKERKGL IFQPVITYLL DRFHDVSILN RSFLVHDGGK NIKMLNYEVL 200
    QRFYSILSNL PTRKPYYSML CCCNDLLKRI TINKGENLQI LQYRWLLIIL 250
    NIPTIRTCLI RDRKSKNVFE TQQIRAVSYE LAKRCIGYLS NLSTKTSQQL 300
    IQSLRRTPTD NFSYQVEILN LYINFQFSRL LSNELSNRTA KNNVKPEDEM 350
    RSRLRRHHTT GHEFLSTRPI SAQSNDKQGS GFTHPVNNKM KFKFFQYEED 400
    WHIHSAAKLT FIYYVANTRR NGRGALSIQS FYNITLDFID YKQDFDHWRG 450
    VAQKTKMNQL IEEWGNSTTK KCFSFCKYPF ILSLGIKISI MEYEIRRIME 500
    HEAEQAFLIS LDKGKSVDVY FKIKVRRDVI SHDSLRCIKE HQGDLLKSLR 550
    IEFVNEPGID AGGLRKEWFF LLTKSLFNPM NGLFIYIKES SRSWFAIDPP 600
    NFDKSKGKNS QLELYYLFGV VMGLAIFNST ILDLQFPKAL YKKLCSEPLS 650
    FEDYSELFPE TSRNLIKMLN YTEDNFEDVF SLTFETTYRN NNWILNDSKS 700
    SKEYVTVELC ENGRNVPITQ SNKHEFVMKW VEFYLEKSIE PQYNKFVSGF 750
    KRVFAECNSI KLFNSEELER LVCGDEEQTK FDFKSLRSVT KYVGGFSDDS 800
    RAVCWFWEII ESWDYPLQKK LLQFVTASDR IPATGISTIP FKISLLGSHD 850
    SDDLPLAHTC FNEICLWNYS SKKKLELKLL WAINESEGYG FR 892
    Length:892
    Mass (Da):103,457
    Last modified:November 1, 1995 - v2
    Checksum:i35EF9A8DDA92BD84
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti362 – 3621H → Q in CAA57291. (PubMed:7957102)Curated
    Sequence conflicti410 – 4101T → M in CAA57291. (PubMed:7957102)Curated
    Sequence conflicti418 – 4181T → I in CAA57291. (PubMed:7957102)Curated
    Sequence conflicti514 – 5196GKSVDV → RQIRRR in CAA57291. (PubMed:7957102)Curated
    Sequence conflicti589 – 60719ESSRS…DKSKG → KKAHVHGLPLTLQILTNQR in CAA57291. (PubMed:7957102)CuratedAdd
    BLAST
    Sequence conflicti705 – 7051V → A in CAA57291. (PubMed:7957102)Curated
    Sequence conflicti723 – 7242KH → ND in CAA57291. (PubMed:7957102)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L36344 Genomic DNA. Translation: AAA88738.1.
    Z49536 Genomic DNA. Translation: CAA89563.1.
    X81635 Genomic DNA. Translation: CAA57291.1.
    BK006943 Genomic DNA. Translation: DAA08825.1.
    PIRiS57055.
    RefSeqiNP_012570.3. NM_001181694.3.

    Genome annotation databases

    EnsemblFungiiYJR036C; YJR036C; YJR036C.
    GeneIDi853494.
    KEGGisce:YJR036C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L36344 Genomic DNA. Translation: AAA88738.1 .
    Z49536 Genomic DNA. Translation: CAA89563.1 .
    X81635 Genomic DNA. Translation: CAA57291.1 .
    BK006943 Genomic DNA. Translation: DAA08825.1 .
    PIRi S57055.
    RefSeqi NP_012570.3. NM_001181694.3.

    3D structure databases

    ProteinModelPortali P40985.
    SMRi P40985. Positions 520-883.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33789. 27 interactions.
    IntActi P40985. 1 interaction.
    MINTi MINT-2782607.
    STRINGi 4932.YJR036C.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJR036C ; YJR036C ; YJR036C .
    GeneIDi 853494.
    KEGGi sce:YJR036C.

    Organism-specific databases

    CYGDi YJR036c.
    SGDi S000003797. HUL4.

    Phylogenomic databases

    eggNOGi COG5021.
    GeneTreei ENSGT00750000117423.
    HOGENOMi HOG000246763.
    KOi K12232.
    OMAi QYGNDWH.
    OrthoDBi EOG70GMQ0.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31673-MONOMER.

    Miscellaneous databases

    NextBioi 974128.

    Gene expression databases

    Genevestigatori P40985.

    Family and domain databases

    InterProi IPR000569. HECT.
    [Graphical view ]
    Pfami PF00632. HECT. 1 hit.
    [Graphical view ]
    SMARTi SM00119. HECTc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56204. SSF56204. 1 hit.
    PROSITEi PS50237. HECT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of a 42.5 kb DNA sequence of chromosome X reveals three tRNA genes and 14 new open reading frames including a gene most probably belonging to the family of ubiquitin-protein ligases."
      Huang M.-E., Chuat J.-C., Galibert F.
      Yeast 11:775-781(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "RAD26, the functional S. cerevisiae homolog of the Cockayne syndrome B gene ERCC6."
      van Gool A.J., Verhage R., Swagemakers S.M.A., van de Putte P., Brouwer J., Troelstra C., Bootsma D., Hoeijmakers J.H.J.
      EMBO J. 13:5361-5369(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 362-892.
    5. "Functional domains of the rsp5 ubiquitin-protein ligase."
      Wang G., Yang J., Huibregtse J.M.
      Mol. Cell. Biol. 19:342-352(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE NAME, GENE DISRUPTION.
    6. "A new yeast poly(A) polymerase complex involved in RNA quality control."
      Vanacova S., Wolf J., Martin G., Blank D., Dettwiler S., Friedlein A., Langen H., Keith G., Keller W.
      PLoS Biol. 3:986-997(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE TRF4 COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION OF THE TRF4 COMPLEX.

    Entry informationi

    Entry nameiHUL4_YEAST
    AccessioniPrimary (citable) accession number: P40985
    Secondary accession number(s): D6VWK9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 113 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3