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P40985

- HUL4_YEAST

UniProt

P40985 - HUL4_YEAST

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Protein

Probable E3 ubiquitin-protein ligase HUL4

Gene
HUL4, YJR036C, J1608
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Probable E3 ubiquitin-protein ligase, component of the TRAMP (TRF4) complex which has a poly(A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism limiting inappropriate expression of genetic information. Polyadenylation is required for the degradative activity of the exosome on several of its nuclear RNA substrates.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei860 – 8601Glycyl thioester intermediate By similarity

GO - Molecular functioni

  1. ligase activity Source: UniProtKB-KW
  2. ubiquitin-protein transferase activity Source: SGD

GO - Biological processi

  1. protein ubiquitination Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-31673-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable E3 ubiquitin-protein ligase HUL4 (EC:6.3.2.-)
Alternative name(s):
HECT ubiquitin ligase 4
Gene namesi
Name:HUL4
Ordered Locus Names:YJR036C
ORF Names:J1608
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJR036c.
SGDiS000003797. HUL4.

Subcellular locationi

Nucleus Inferred

GO - Cellular componenti

  1. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 892892Probable E3 ubiquitin-protein ligase HUL4PRO_0000084094Add
BLAST

Expressioni

Gene expression databases

GenevestigatoriP40985.

Interactioni

Subunit structurei

Component of the TRAMP complex (also called TRF4 complex) composed of at least HUL4, MTR4, PAP2/TRF4 and either AIR1 or AIR2.1 Publication

Protein-protein interaction databases

BioGridi33789. 27 interactions.
IntActiP40985. 1 interaction.
MINTiMINT-2782607.
STRINGi4932.YJR036C.

Structurei

3D structure databases

ProteinModelPortaliP40985.
SMRiP40985. Positions 520-883.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini792 – 892101HECTAdd
BLAST

Sequence similaritiesi

Belongs to the HUL4 family.

Phylogenomic databases

eggNOGiCOG5021.
GeneTreeiENSGT00750000117423.
HOGENOMiHOG000246763.
KOiK12232.
OMAiQYGNDWH.
OrthoDBiEOG70GMQ0.

Family and domain databases

InterProiIPR000569. HECT.
[Graphical view]
PfamiPF00632. HECT. 1 hit.
[Graphical view]
SMARTiSM00119. HECTc. 1 hit.
[Graphical view]
SUPFAMiSSF56204. SSF56204. 1 hit.
PROSITEiPS50237. HECT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P40985-1 [UniParc]FASTAAdd to Basket

« Hide

MVSLFDKLNA KKDGRDGSVS KELLSHSVAH TKNRLPKSGR RTSERSLAAS    50
VKDGSCSNSK SNKRNSSASV SGEEDKSCLI SLNCLCCGVP LRFPASITKF 100
RCSACQVTVI VKEPEINSNL ESSTHISCTL EGLQMVVRRC HDDLQRLKKT 150
GILDKERKGL IFQPVITYLL DRFHDVSILN RSFLVHDGGK NIKMLNYEVL 200
QRFYSILSNL PTRKPYYSML CCCNDLLKRI TINKGENLQI LQYRWLLIIL 250
NIPTIRTCLI RDRKSKNVFE TQQIRAVSYE LAKRCIGYLS NLSTKTSQQL 300
IQSLRRTPTD NFSYQVEILN LYINFQFSRL LSNELSNRTA KNNVKPEDEM 350
RSRLRRHHTT GHEFLSTRPI SAQSNDKQGS GFTHPVNNKM KFKFFQYEED 400
WHIHSAAKLT FIYYVANTRR NGRGALSIQS FYNITLDFID YKQDFDHWRG 450
VAQKTKMNQL IEEWGNSTTK KCFSFCKYPF ILSLGIKISI MEYEIRRIME 500
HEAEQAFLIS LDKGKSVDVY FKIKVRRDVI SHDSLRCIKE HQGDLLKSLR 550
IEFVNEPGID AGGLRKEWFF LLTKSLFNPM NGLFIYIKES SRSWFAIDPP 600
NFDKSKGKNS QLELYYLFGV VMGLAIFNST ILDLQFPKAL YKKLCSEPLS 650
FEDYSELFPE TSRNLIKMLN YTEDNFEDVF SLTFETTYRN NNWILNDSKS 700
SKEYVTVELC ENGRNVPITQ SNKHEFVMKW VEFYLEKSIE PQYNKFVSGF 750
KRVFAECNSI KLFNSEELER LVCGDEEQTK FDFKSLRSVT KYVGGFSDDS 800
RAVCWFWEII ESWDYPLQKK LLQFVTASDR IPATGISTIP FKISLLGSHD 850
SDDLPLAHTC FNEICLWNYS SKKKLELKLL WAINESEGYG FR 892
Length:892
Mass (Da):103,457
Last modified:November 1, 1995 - v2
Checksum:i35EF9A8DDA92BD84
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti362 – 3621H → Q in CAA57291. 1 Publication
Sequence conflicti410 – 4101T → M in CAA57291. 1 Publication
Sequence conflicti418 – 4181T → I in CAA57291. 1 Publication
Sequence conflicti514 – 5196GKSVDV → RQIRRR in CAA57291. 1 Publication
Sequence conflicti589 – 60719ESSRS…DKSKG → KKAHVHGLPLTLQILTNQR in CAA57291. 1 PublicationAdd
BLAST
Sequence conflicti705 – 7051V → A in CAA57291. 1 Publication
Sequence conflicti723 – 7242KH → ND in CAA57291. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L36344 Genomic DNA. Translation: AAA88738.1.
Z49536 Genomic DNA. Translation: CAA89563.1.
X81635 Genomic DNA. Translation: CAA57291.1.
BK006943 Genomic DNA. Translation: DAA08825.1.
PIRiS57055.
RefSeqiNP_012570.3. NM_001181694.3.

Genome annotation databases

EnsemblFungiiYJR036C; YJR036C; YJR036C.
GeneIDi853494.
KEGGisce:YJR036C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L36344 Genomic DNA. Translation: AAA88738.1 .
Z49536 Genomic DNA. Translation: CAA89563.1 .
X81635 Genomic DNA. Translation: CAA57291.1 .
BK006943 Genomic DNA. Translation: DAA08825.1 .
PIRi S57055.
RefSeqi NP_012570.3. NM_001181694.3.

3D structure databases

ProteinModelPortali P40985.
SMRi P40985. Positions 520-883.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33789. 27 interactions.
IntActi P40985. 1 interaction.
MINTi MINT-2782607.
STRINGi 4932.YJR036C.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJR036C ; YJR036C ; YJR036C .
GeneIDi 853494.
KEGGi sce:YJR036C.

Organism-specific databases

CYGDi YJR036c.
SGDi S000003797. HUL4.

Phylogenomic databases

eggNOGi COG5021.
GeneTreei ENSGT00750000117423.
HOGENOMi HOG000246763.
KOi K12232.
OMAi QYGNDWH.
OrthoDBi EOG70GMQ0.

Enzyme and pathway databases

BioCyci YEAST:G3O-31673-MONOMER.

Miscellaneous databases

NextBioi 974128.

Gene expression databases

Genevestigatori P40985.

Family and domain databases

InterProi IPR000569. HECT.
[Graphical view ]
Pfami PF00632. HECT. 1 hit.
[Graphical view ]
SMARTi SM00119. HECTc. 1 hit.
[Graphical view ]
SUPFAMi SSF56204. SSF56204. 1 hit.
PROSITEi PS50237. HECT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of a 42.5 kb DNA sequence of chromosome X reveals three tRNA genes and 14 new open reading frames including a gene most probably belonging to the family of ubiquitin-protein ligases."
    Huang M.-E., Chuat J.-C., Galibert F.
    Yeast 11:775-781(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "RAD26, the functional S. cerevisiae homolog of the Cockayne syndrome B gene ERCC6."
    van Gool A.J., Verhage R., Swagemakers S.M.A., van de Putte P., Brouwer J., Troelstra C., Bootsma D., Hoeijmakers J.H.J.
    EMBO J. 13:5361-5369(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 362-892.
  5. "Functional domains of the rsp5 ubiquitin-protein ligase."
    Wang G., Yang J., Huibregtse J.M.
    Mol. Cell. Biol. 19:342-352(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE NAME, GENE DISRUPTION.
  6. "A new yeast poly(A) polymerase complex involved in RNA quality control."
    Vanacova S., Wolf J., Martin G., Blank D., Dettwiler S., Friedlein A., Langen H., Keith G., Keller W.
    PLoS Biol. 3:986-997(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE TRF4 COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION OF THE TRF4 COMPLEX.

Entry informationi

Entry nameiHUL4_YEAST
AccessioniPrimary (citable) accession number: P40985
Secondary accession number(s): D6VWK9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1995
Last sequence update: November 1, 1995
Last modified: September 3, 2014
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

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